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Protein

Beta sliding clamp

Gene

dnaN

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:RC0583
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054571 – 379Beta sliding clampAdd BLAST379

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi8 – 21Combined sources14
Helixi29 – 31Combined sources3
Beta strandi33 – 39Combined sources7
Beta strandi42 – 48Combined sources7
Beta strandi50 – 59Combined sources10
Beta strandi61 – 64Combined sources4
Beta strandi66 – 71Combined sources6
Helixi72 – 79Combined sources8
Beta strandi85 – 91Combined sources7
Beta strandi97 – 101Combined sources5
Beta strandi104 – 108Combined sources5
Helixi113 – 115Combined sources3
Beta strandi125 – 131Combined sources7
Helixi132 – 140Combined sources9
Helixi143 – 145Combined sources3
Helixi153 – 155Combined sources3
Beta strandi156 – 163Combined sources8
Beta strandi166 – 172Combined sources7
Beta strandi174 – 183Combined sources10
Beta strandi191 – 196Combined sources6
Helixi197 – 207Combined sources11
Turni210 – 214Combined sources5
Beta strandi215 – 221Combined sources7
Beta strandi223 – 230Combined sources8
Turni231 – 233Combined sources3
Beta strandi234 – 239Combined sources6
Helixi249 – 251Combined sources3
Beta strandi257 – 263Combined sources7
Helixi264 – 275Combined sources12
Beta strandi285 – 289Combined sources5
Beta strandi291 – 300Combined sources10
Turni301 – 303Combined sources3
Beta strandi304 – 311Combined sources8
Helixi316 – 318Combined sources3
Beta strandi320 – 322Combined sources3
Beta strandi328 – 332Combined sources5
Helixi334 – 343Combined sources10
Beta strandi347 – 353Combined sources7
Beta strandi360 – 364Combined sources5
Beta strandi367 – 374Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W7ZX-ray1.70A/B1-379[»]
ProteinModelPortaliQ92I37.
SMRiQ92I37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

HOGENOMiHOG000071791.
KOiK02338.
OMAiNYEAVIP.
OrthoDBiPOG091H02B3.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478. PTHR30478. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92I37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLIVETKT LVQSLGFASS VVEKRNVIPE YANIKLSAKD GNLELSSTNM
60 70 80 90 100
DLYLSQKIAV QVVSEGECTV STKTLNDIVR KLPDSELTLT DLGTTGLEIK
110 120 130 140 150
GKNCKFNLFT LPVSSFPAMD SINPEASFKI SCTDFAKIIE STKFSISLDE
160 170 180 190 200
TRYNLNGVYL HIKDKEFCSA STDGHRLSIS WVTLEKQIKN FGVILPQKSA
210 220 230 240 250
EEILKIVKDP KNINEDIEIL LSSNKIKFIC NENTSMLSKL IDGTFPDYST
260 270 280 290 300
FIPESSSSKL VINRKMFADS IERIAIITVE KFRAVKLSLS RETLEISAVG
310 320 330 340 350
EARGNAKEVI NSSQDKESFY EYNSDESLAI GFNPQYLEDV LKAVKSDVVE
360 370
LYFSDVSAPV LIKFPENPKD IFVVMPVKV
Length:379
Mass (Da):42,237
Last modified:December 1, 2001 - v1
Checksum:i1FBE1A62CE25639C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03121.1.
PIRiG97772.
RefSeqiWP_010977217.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03121; AAL03121; RC0583.
GeneIDi927682.
KEGGirco:RC0583.
PATRICifig|272944.4.peg.665.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_RICCN
AccessioniPrimary (citable) accession number: Q92I37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: December 20, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families