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Q92I25

- DAPA_RICCN

UniProt

Q92I25 - DAPA_RICCN

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Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene
dapA, RC0595
Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 461Part of a proton relay during catalysis By similarity
Binding sitei47 – 471Pyruvate By similarity
Sitei109 – 1091Part of a proton relay during catalysis By similarity
Active sitei135 – 1351Proton donor/acceptor By similarity
Active sitei163 – 1631Schiff-base intermediate with substrate By similarity
Binding sitei205 – 2051Pyruvate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase (EC:4.3.3.7)
Short name:
HTPA synthase
Gene namesi
Name:dapA
Ordered Locus Names:RC0595
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2942944-hydroxy-tetrahydrodipicolinate synthaseUniRule annotationPRO_0000103143Add
BLAST

Interactioni

Subunit structurei

Homotetramer; dimer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272944.RC0595.

Structurei

3D structure databases

ProteinModelPortaliQ92I25.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiMLYSGDD.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92I25-1 [UniParc]FASTAAdd to Basket

« Hide

MHNIFKGLIT ALITPFKDNK LDLYALERIV KHQIKHEVDA VLIAGSTGES    50
SSLSFEEYKL LLQTSVEIVN KCIPIISGCS SNNTTYARAL AAESTKIGVD 100
GFMASPPSYV KPTQHGIYKH FEALHEACNL PIMLYSAPTR SGVDFSDETI 150
LRLSKLPRIL ALKDCGVDLE RPLRIRATVK KDFNILTGND EVVLAFNAQG 200
GVGWTSVASN IVPNICKELL EKWNKNDTKG ALEIHQKLLP LYTALFVESN 250
PIPIKYAAHY LGLCENEIRP PLTEASDSAK KQIENIITSL SIKI 294
Length:294
Mass (Da):32,476
Last modified:December 1, 2001 - v1
Checksum:iF813B480E49BD0C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03133.1.
PIRiC97774.
RefSeqiNP_360232.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03133; AAL03133; RC0595.
GeneIDi927692.
KEGGirco:RC0595.
PATRICi17888458. VBIRicCon45613_0680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03133.1 .
PIRi C97774.
RefSeqi NP_360232.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92I25.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272944.RC0595.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL03133 ; AAL03133 ; RC0595 .
GeneIDi 927692.
KEGGi rco:RC0595.
PATRICi 17888458. VBIRicCon45613_0680.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi MLYSGDD.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiDAPA_RICCN
AccessioniPrimary (citable) accession number: Q92I25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi