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Protein

Cysteine desulfurase IscS

Gene

iscS

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.UniRule annotation

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei334 – 3341Cysteine persulfide intermediateUniRule annotation

GO - Molecular functioni

  1. cysteine desulfurase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cysteine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase IscSUniRule annotation (EC:2.8.1.7UniRule annotation)
Gene namesi
Name:iscSUniRule annotation
Synonyms:spl1
Ordered Locus Names:RC0731
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Cysteine desulfurase IscSPRO_0000150275Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111N6-(pyridoxal phosphate)lysineUniRule annotation

Proteomic databases

PRIDEiQ92HP1.

Interactioni

Subunit structurei

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.UniRule annotation

Protein-protein interaction databases

STRINGi272944.RC0730.

Structurei

3D structure databases

ProteinModelPortaliQ92HP1.
SMRiQ92HP1. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1104.
HOGENOMiHOG000017510.
KOiK04487.
OMAiAEKMMQC.
OrthoDBiEOG62RSBK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92HP1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPQLNNLTL PIYMDYQATT PIDPRVMEAM LPYFTTKFGN PHSRSHSFGW
60 70 80 90 100
EAENAVEEAR SMVAKLIGAD TKEIIFTSGA TESNNLAIKG IAKFYSNKKN
110 120 130 140 150
HIITVVSEHK CVLDACRHLE QEGIKITYLP IKPNGIIDLE TLKNAITDQT
160 170 180 190 200
MLVSVMVVNN EIGVVQPLKE IGKICREKGV FFHSDIAQGF GKIPIDVNAF
210 220 230 240 250
NIDLASISGH KIYGPKGIGA LYVRKKPRVR VTPLINGGGQ ERGMRSGTLP
260 270 280 290 300
TPLIVGLGMA AEIAYSEMEK DTKHVNYLFD RFLNNIHKRI SEVYLNGDKN
310 320 330 340 350
QRYKGNLNLS FAGVEGESMI LAIKDLAVSS GSACTSASLE PSYVLRSMGI
360 370 380 390 400
GEELAHTAIR FGIGRFTTEQ EVDYAVNLIC SKIDKLRELS PLWEMMQEGI
410
DLKKIKWAVH
Length:410
Mass (Da):45,475
Last modified:December 1, 2001 - v1
Checksum:i930AB52556C2F9BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03268.1.
PIRiB97791.
RefSeqiNP_360367.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03268; AAL03268; RC0730.
GeneIDi928569.
KEGGirco:RC0730.
PATRICi17888774. VBIRicCon45613_0832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03268.1.
PIRiB97791.
RefSeqiNP_360367.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92HP1.
SMRiQ92HP1. Positions 9-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272944.RC0730.

Proteomic databases

PRIDEiQ92HP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL03268; AAL03268; RC0730.
GeneIDi928569.
KEGGirco:RC0730.
PATRICi17888774. VBIRicCon45613_0832.

Phylogenomic databases

eggNOGiCOG1104.
HOGENOMiHOG000017510.
KOiK04487.
OMAiAEKMMQC.
OrthoDBiEOG62RSBK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiISCS_RICCN
AccessioniPrimary (citable) accession number: Q92HP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.