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Q92HP1 (ISCS_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine desulfurase IscS

EC=2.8.1.7
Gene names
Name:iscS
Synonyms:spl1
Ordered Locus Names:RC0731
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins By similarity. HAMAP-Rule MF_00331

Catalytic activity

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor. HAMAP-Rule MF_00331

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00331

Subunit structure

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00331.

Sequence similarities

Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine desulfurase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Cysteine desulfurase IscS HAMAP-Rule MF_00331
PRO_0000150275

Sites

Active site3341Cysteine persulfide intermediate By similarity

Amino acid modifications

Modified residue2111N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92HP1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 930AB52556C2F9BE

FASTA41045,475
        10         20         30         40         50         60 
MNPQLNNLTL PIYMDYQATT PIDPRVMEAM LPYFTTKFGN PHSRSHSFGW EAENAVEEAR 

        70         80         90        100        110        120 
SMVAKLIGAD TKEIIFTSGA TESNNLAIKG IAKFYSNKKN HIITVVSEHK CVLDACRHLE 

       130        140        150        160        170        180 
QEGIKITYLP IKPNGIIDLE TLKNAITDQT MLVSVMVVNN EIGVVQPLKE IGKICREKGV 

       190        200        210        220        230        240 
FFHSDIAQGF GKIPIDVNAF NIDLASISGH KIYGPKGIGA LYVRKKPRVR VTPLINGGGQ 

       250        260        270        280        290        300 
ERGMRSGTLP TPLIVGLGMA AEIAYSEMEK DTKHVNYLFD RFLNNIHKRI SEVYLNGDKN 

       310        320        330        340        350        360 
QRYKGNLNLS FAGVEGESMI LAIKDLAVSS GSACTSASLE PSYVLRSMGI GEELAHTAIR 

       370        380        390        400        410 
FGIGRFTTEQ EVDYAVNLIC SKIDKLRELS PLWEMMQEGI DLKKIKWAVH 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL03268.1.
PIRB97791.
RefSeqNP_360367.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92HP1.
SMRQ92HP1. Positions 9-398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC0730.

Proteomic databases

PRIDEQ92HP1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03268; AAL03268; RC0730.
GeneID928569.
KEGGrco:RC0730.
PATRIC17888774. VBIRicCon45613_0832.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1104.
HOGENOMHOG000017510.
KOK04487.
OMAYLPVQKN.
OrthoDBEOG62RSBK.
ProtClustDBPRK14012.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00331. Cys_desulf_aminotr_5.
InterProIPR000192. Aminotrans_V/Cys_dSase.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase.
IPR016454. Cysteine_dSase_NifS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFPIRSF005572. NifS. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR02006. IscS. 1 hit.
PROSITEPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISCS_RICCN
AccessionPrimary (citable) accession number: Q92HP1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names