Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q92H58 (MFD_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription-repair-coupling factor
Short name=TRCF
EC=3.6.4.-
Alternative name(s):
ATP-dependent helicase mfd
Gene names
Name:mfd
Ordered Locus Names:RC0913
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length1122 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Necessary for strand-specific repair. A lesion in the template strand blocks the RNA polymerase complex (RNAP). The RNAP-DNA-RNA complex is specifically recognized by TRCF which releases RNAP and the truncated transcript; the TCRF may replace RNAP at the lesion site and then recruit the UvrA/B/C repair system By similarity.

Sequence similarities

In the N-terminal section; belongs to the UvrB family.

In the C-terminal section; belongs to the helicase family. RecG subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

damaged DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11221122Transcription-repair-coupling factor
PRO_0000281072

Regions

Domain593 – 758166Helicase ATP-binding
Domain779 – 935157Helicase C-terminal
Nucleotide binding606 – 6138ATP Potential
Motif711 – 7144DEEQ box

Sequences

Sequence LengthMass (Da)Tools
Q92H58 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: AA303708D4815E79

FASTA1,122127,560
        10         20         30         40         50         60 
MLQQKFPAAA KSFFAIDNFT KNLKQDFILS ASNEEEALQL YKQALFFSSN ENIYYFPSYN 

        70         80         90        100        110        120 
TIPYDHTSPN ANILSRRAET LIKLTTNNSN SNKLLITHTA NLLNKLPPKD FFSKYFLKLS 

       130        140        150        160        170        180 
PKMKFTTDEL AMFLVENSFT RNASSIDVGE FAVRGEIIDI ILSGPKAYRI HFSWGYIESI 

       190        200        210        220        230        240 
KEFDIDTQIS TKSCRELIIS PANEIVLNSE TIGNFKNNYL RNFGVNHTDN ALYEAVISGR 

       250        260        270        280        290        300 
KFTGYEQLLP LFYDSCSNLI DYLNDPIFIF DNLSKKAILE FEHSYNDFYS ARSEANKLKF 

       310        320        330        340        350        360 
NSFYPTLSPT SLYFTASEIT ELLEQKNNIL LTFENSEQAS LIKNIAATSF IEKKTVFDKL 

       370        380        390        400        410        420 
FEVIKANSHK KIIIGSSVLS SFERIKSIIQ NYEYKYNEIN KLDEAKASII NVAIIPLNQS 

       430        440        450        460        470        480 
FYTKEYLFIT ASELLEEKPS STNTNKKLKN ILLELDNLAE GEFVVHKDHG IGQFLKLEAL 

       490        500        510        520        530        540 
EIKGKPHDFL KILYAGNDKL YIPVESIEVI KKYGNDNAEL DKLGSVSWQR SKAKLKKRIK 

       550        560        570        580        590        600 
EIALHLIQIA AKRKLNSSAS VEFDLEEYDK FCANFPFSET EDQLIAINDI KEDLRNGMLM 

       610        620        630        640        650        660 
DRLICGDVGF GKTEVAMRAV FMVAKSLNEH LPQVAVVVPT TILCSQHFSR FIERFKGFGL 

       670        680        690        700        710        720 
NIKQLSSVIS SKEAKIIRSE LESGKINIII GTHSLLHKNI KFFNLKLLII DEEQHFGVGQ 

       730        740        750        760        770        780 
KEFLKSLKSS SHVLAMSATP IPRTLQMSMT GLKELSIIAT PPLNRLEVHT SVMPYDPVII 

       790        800        810        820        830        840 
RDALLREHFR GGRSFYVVPR IKDIEDIAKQ LKQIVPELSY KIAYGKMTPS KIDEVMSEFY 

       850        860        870        880        890        900 
AGKFDILVST TIIESGIDIA EANTMIIHNA DMLGLSQLYQ LRGRIGRGKM RGYAYLTVAS 

       910        920        930        940        950        960 
HKKMTSHSLR RLEIIQNSCA LGSGFTIASR DMDLRGFGNL IGEEQSGQIK EVGTELYQEM 

       970        980        990       1000       1010       1020 
LEEQIAIFKD ESIVSEQPFI PTINLGLSVF IPDNYVADAA LKLGLYRRIG NLSNEIEVET 

      1030       1040       1050       1060       1070       1080 
FKDEMIDRFG LLPIEFNNLL DIVKIKLLCF KLNIENLDSG DNGFVIKFYK NADMTDKILK 

      1090       1100       1110       1120 
FVTTYSNQAK IKPDNKLVYI KKLVDKNIIV EANQLLWNLS EV

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006914 Genomic DNA. Translation: AAL03451.1.
PIRA97814.
RefSeqNP_360550.1. NC_003103.1.

3D structure databases

HSSPHSSP built from PDB template 2EYQ based on UniProtKB P30958.
ProteinModelPortalQ92H58.
ModBaseSearch...

Protein-protein interaction databases

STRING272944.RC0913.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03451; AAL03451; RC0913.
GeneID928843.
KEGGrco:RC0913.
PATRIC17889198. VBIRicCon45613_1038.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1197.
HOGENOMHOG000216592.
KOK03723.
OMAVIIRDAL.
ProtClustDBCLSK871007.

Family and domain databases

InterProIPR003711. CarD-like/TRCF_domain.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004576. Mfd.
IPR005118. Tscpt_repair-coupling_fac_dom.
[Graphical view]
PfamPF02559. CarD_CdnL_TRCF. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF03461. TRCF. 1 hit.
[Graphical view]
SMARTSM01058. CarD_TRCF. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00982. TRCF. 1 hit.
[Graphical view]
SUPFAMSSF141259. SSF141259. 1 hit.
TIGRFAMsTIGR00580. mfd. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMFD_RICCN
AccessionPrimary (citable) accession number: Q92H58
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: December 1, 2001
Last modified: May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia conorii

(strain Malish 7): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents