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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei616 – 6161ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RC0953
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10921092Isoleucine--tRNA ligasePRO_0000098558Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ92H19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi613 – 6175"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92H19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFAGLE REILKFWQDN NIFQKSIDDR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTIK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR LAIANFGIEK FNAHCRASVM KYANDWEEYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFVLS HPVTTTTGSF KEYRILAWTT TPWTLPSNLA
260 270 280 290 300
LAVGSDIDYA LVPKNDVCYI IAAYSVSKYA KELGLSGEEN FEIIKGSALQ
310 320 330 340 350
GLNYKSLFDY FENHPNSFKI FAGDFVVEGD GTGVVHMAPG FGEDDQILCE
360 370 380 390 400
SKGIELVCPV DNSGKFTKEI PDLEGLQVFD ANDKIIIKLK EQGNWLKTEQ
410 420 430 440 450
YIHNYPHCWR TDTPLIYKAV PSWYVKVTQF KDRMVELNQQ INWIPFHVKD
460 470 480 490 500
NLFGKWLENA RDWSISRNRF WGTPLPVWKS DDPKYPRIDV YGSIEELEKD
510 520 530 540 550
FGVKVTDLHR PFIDELTRPN PDDPTGKSTM RRIEDVFDCW FESGSMPYGQ
560 570 580 590 600
AHYPFENKEW FEDHFPADFI VEYSAQTRGW FYTLMVLSTA LFDRPPFLNC
610 620 630 640 650
ICHGVILDST GQKLSKRLNN YADPLELFDK YGSDALRVTM LSSNVVKGQE
660 670 680 690 700
LLIDKDGKMV FDTLRLFIKP IWNAYHFFTM YANADSLKGK LNFSSKNVLD
710 720 730 740 750
VYILSKLKIA VQKIEESLDN FDTQTAYHAV SAFFEVLNNW YIRRSRARFW
760 770 780 790 800
KSAKDTDKQN AYNTLYSCLD TMAIAMSALV PMISEAIYKG LRHCEERNDT
810 820 830 840 850
ALSGKSNIIA RKDTSLDKAI SGVSHKIATA LSVPRNDAIS VHLCNYPTLS
860 870 880 890 900
DFEINHELVA TMDNVLDICS NSLFIRSTEN IRVRQPLASI AIISKHNNNL
910 920 930 940 950
KDFEDLIKDE INVKAVIYRD DLENYASKKL SINFPMLGKR LPHKMKEIIA
960 970 980 990 1000
ASKKGEWEAI TGGLAICGET LNSDEYKLVL EPYSHIKGAA SFENNSSLLI
1010 1020 1030 1040 1050
LDLELTPELI EEGYARDIVR FIQQARKDAD FSITDRILIE IISEFNLSKI
1060 1070 1080 1090
IDNYGDFIKE QTLGEFAKNF TPDYVSKVAL ENHQIQLKVK KS
Length:1,092
Mass (Da):125,342
Last modified:December 20, 2005 - v2
Checksum:i97E4CE3E51AB91DD
GO

Sequence cautioni

The sequence AAL03491.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03491.1. Different initiation.
PIRiA97819.
RefSeqiWP_010977549.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03491; AAL03491; RC0953.
GeneIDi927861.
KEGGirco:RC0953.
PATRICi17889296. VBIRicCon45613_1086.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03491.1. Different initiation.
PIRiA97819.
RefSeqiWP_010977549.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92H19.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL03491; AAL03491; RC0953.
GeneIDi927861.
KEGGirco:RC0953.
PATRICi17889296. VBIRicCon45613_1086.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiSYI_RICCN
AccessioniPrimary (citable) accession number: Q92H19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: July 22, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.