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Q92H06 (SYE2_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 2

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2
Gene names
Name:gltX2
Ordered Locus Names:RC0966
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 RPE1 insert domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022
PRO_0000119639

Regions

Domain335 – 38349RPE1 insert
Motif11 – 2111"HIGH" region HAMAP-Rule MF_00022
Motif240 – 2445"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2431ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92H06 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5465C5274751C5A1

FASTA51358,453
        10         20         30         40         50         60 
MNNNVITRFA PSPTGFLHIG SARTALFNYL FARHHNGKFL LRIEDTDKER STKEAVEAIF 

        70         80         90        100        110        120 
SGLKWLGLDW NGEVIFQSKR NNLYKEAALK LLQNGKAYYC FTRQEEIERQ RQQALENKQH 

       130        140        150        160        170        180 
FIFNSEWRDK DPSIYPTDIK PVIRLKTPRE GSITIHDTLQ GEVVIENSHI DDMVLLRADG 

       190        200        210        220        230        240 
TATYMLAVVV DDHDMGITHI IRGDDHLTNT ARQLAIYQAF GYAVPSMTHI PLIHGADGAK 

       250        260        270        280        290        300 
LSKRHGALGI EAYKDMGYLP ESLCNYLLRL GWSHGDDEII SMTQAIDWFN LDSLGKSPSK 

       310        320        330        340        350        360 
LDFAKMNSLN AHYLRMLDND SLTSKTVEIL EQNYNTLLRH LPYREEFGGN TERSTAAYID 

       370        380        390        400        410        420 
IREDASTGLT YKLPLAVELP KKFKISEQEI GYIKQAMPSL LVRSETLLEL TRLAQIYLVD 

       430        440        450        460        470        480 
SPIIYSQDSK EIIENCDKNL IKQIIENLSE LEQFDKESVQ NKFKEIAAAN DLKLNDIMKP 

       490        500        510 
VRALITGMTA SPSIFEIAEI LGKENILKRL KII 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL03504.1.
PIRF97820.
RefSeqNP_360603.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92H06.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC0966.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03504; AAL03504; RC0966.
GeneID927885.
KEGGrco:RC0966.
PATRIC17889322. VBIRicCon45613_1099.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252722.
KOK01885.
OMAIQECIND.
OrthoDBEOG6DRPF7.
ProtClustDBPRK14895.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR005728. Rickett_RPE.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 2 hits.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
TIGR01045. RPE1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE2_RICCN
AccessionPrimary (citable) accession number: Q92H06
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries