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Q92GZ0 (RPOA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit alpha

Short name=RNAP subunit alpha
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha
Gene names
Name:rpoA
Ordered Locus Names:RC0982
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00059

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059

Subunit structure

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Domain

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP-Rule MF_00059

Sequence similarities

Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340DNA-directed RNA polymerase subunit alpha HAMAP-Rule MF_00059
PRO_0000175368

Regions

Region1 – 236236Alpha N-terminal domain (alpha-NTD) By similarity
Region251 – 34090Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92GZ0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 65ABCAF249450DEC

FASTA34038,306
        10         20         30         40         50         60 
MLSLSKNWNT LIKPNKVAYE NFPETNNKAK IVVEPLERGF GLTLGNAMRR VLLSSLQGAA 

        70         80         90        100        110        120 
ITSIKIPAIE HEFSSIPGVK EDVSEVILNI KGIEVKMHVA EKRIMKLKAT GPCVVTAGMI 

       130        140        150        160        170        180 
ETGHDVEILN PDHVICDLAK DKQLEMELTC KVGKGYVLST NSYEDNLPIG EIAIDALFNP 

       190        200        210        220        230        240 
VKSVTYKVEN TRVGQVTDYD KLIMFVETNG DVLPEMAVGL AARILQEQLQ LFISFEEQEE 

       250        260        270        280        290        300 
DKQVKTDTLP FSPYLLKRVD ELELSVRSAN CLKNDNIIYI GDLVKRTESD MLRTPNFGRK 

       310        320        330        340 
SLNEIKEILA KFNLRFGMDV PDWPPENIQE LSKRYEDSYN 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL03520.1.
PIRF97822.
RefSeqNP_360619.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92GZ0.
SMRQ92GZ0. Positions 253-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC0982.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03520; AAL03520; RC0982.
GeneID928124.
KEGGrco:RC0982.
PATRIC17889370. VBIRicCon45613_1122.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0202.
HOGENOMHOG000218481.
KOK03040.
OMAKAESIHY.
OrthoDBEOG68WR84.

Family and domain databases

Gene3D2.170.120.12. 1 hit.
HAMAPMF_00059. RNApol_bact_RpoA.
InterProIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsTIGR02027. rpoA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRPOA_RICCN
AccessionPrimary (citable) accession number: Q92GZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names