Q92GZ0 (RPOA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit alpha
Short name=RNAP subunit alpha
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha

Gene names

Name:	rpoA
Ordered Locus Names:	RC0982

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia conorii

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059
Subunit structure	Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Domain	The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP-Rule MF_00059
Sequence similarities	Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	DNA-directed RNA polymerase subunit alpha HAMAP-Rule MF_00059		PRO_0000175368
Regions
	Region	1 – 236	236	Alpha N-terminal domain (alpha-NTD) By similarity
	Region	251 – 340	90	Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92GZ0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 65ABCAF249450DEC	FASTA	340	38,306
	10 20 30 40 50 60 MLSLSKNWNT LIKPNKVAYE NFPETNNKAK IVVEPLERGF GLTLGNAMRR VLLSSLQGAA 70 80 90 100 110 120 ITSIKIPAIE HEFSSIPGVK EDVSEVILNI KGIEVKMHVA EKRIMKLKAT GPCVVTAGMI 130 140 150 160 170 180 ETGHDVEILN PDHVICDLAK DKQLEMELTC KVGKGYVLST NSYEDNLPIG EIAIDALFNP 190 200 210 220 230 240 VKSVTYKVEN TRVGQVTDYD KLIMFVETNG DVLPEMAVGL AARILQEQLQ LFISFEEQEE 250 260 270 280 290 300 DKQVKTDTLP FSPYLLKRVD ELELSVRSAN CLKNDNIIYI GDLVKRTESD MLRTPNFGRK 310 320 330 340 SLNEIKEILA KFNLRFGMDV PDWPPENIQE LSKRYEDSYN

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL03520.1.
PIR	F97822.
RefSeq	NP_360619.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92GZ0.
SMR	Q92GZ0. Positions 253-323.
ModBase	Search...
Protein-protein interaction databases
STRING	272944.RC0982.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL03520; AAL03520; RC0982.
GeneID	928124.
KEGG	rco:RC0982.
PATRIC	17889370. VBIRicCon45613_1122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0202.
HOGENOM	HOG000218481.
KO	K03040.
OMA	GKKSYDE.
ProtClustDB	PRK05182.
Family and domain databases
Gene3D	2.170.120.12. 1 hit.
HAMAP	MF_00059. RNApol_bact_RpoA.
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011773. DNA-dir_RpoA. IPR011260. RNAP_asu_C. [Graphical view]
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
ProDom	PD001179. RNAP_asu_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
SUPFAM	SSF47789. RNAP_alpha_C. 1 hit. SSF56553. RNAP_insert. 1 hit. SSF55257. RNAP_RBP11-like. 1 hit.
TIGRFAMs	TIGR02027. rpoA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOA_RICCN

Accession

Primary (citable) accession number: Q92GZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program