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Q92GM6

- TGT_RICCN

UniProt

Q92GM6 - TGT_RICCN

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Protein
Queuine tRNA-ribosyltransferase
Gene
tgt, RC1097
Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921Nucleophile By similarity
Binding sitei93 – 931Substrate By similarity
Metal bindingi304 – 3041Zinc By similarity
Metal bindingi306 – 3061Zinc By similarity
Metal bindingi309 – 3091Zinc By similarity
Metal bindingi335 – 3351Zinc By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:tgt
Ordered Locus Names:RC1097
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Queuine tRNA-ribosyltransferaseUniRule annotation
PRO_0000135513Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272944.RC1097.

Structurei

3D structure databases

ProteinModelPortaliQ92GM6.
SMRiQ92GM6. Positions 3-355.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0343.
HOGENOMiHOG000223473.
KOiK00773.
OMAiFGSDIIM.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92GM6-1 [UniParc]FASTAAdd to Basket

« Hide

MSKFSFNIHH QHKKARSGII VTAHGEMRTP AFMPVGTRGT VKAMLPESVA    50
ETGADILLGN TYHLMLQPTA ERIVQLGGLH KFMNWDKPIL TDSGGFQVMS 100
LSKLCKITEE GVSFSSHING DKYMLTPERS TEIQYLLGST ITMAFDECTP 150
YPATFEEAKT SMQLTTRWAN RSRNAFVKRE GYAQFGIIQG SVYEELREQS 200
AKDLVELDFE GYAIGGLAVG EGQELMFKVL DYAPEFLPQN KPRYLMGVGK 250
PVDIIGAVSR GIDMFDCVIP TRSGRNGQAF TKYGTVNIRN SKYADDNKPL 300
EHDCLCPACR NYSKAYLHHL VRIGEILGSM LMTWHNLTYF QNLMSRIRAY 350
IKLGKDFDFD S 361
Length:361
Mass (Da):40,590
Last modified:December 1, 2001 - v1
Checksum:i1BEC5C2C39341A84
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03635.1.
PIRiA97837.
RefSeqiNP_360734.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03635; AAL03635; RC1097.
GeneIDi928246.
KEGGirco:RC1097.
PATRICi17889654. VBIRicCon45613_1260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03635.1 .
PIRi A97837.
RefSeqi NP_360734.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92GM6.
SMRi Q92GM6. Positions 3-355.
ModBasei Search...

Protein-protein interaction databases

STRINGi 272944.RC1097.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL03635 ; AAL03635 ; RC1097 .
GeneIDi 928246.
KEGGi rco:RC1097.
PATRICi 17889654. VBIRicCon45613_1260.

Phylogenomic databases

eggNOGi COG0343.
HOGENOMi HOG000223473.
KOi K00773.
OMAi FGSDIIM.
OrthoDBi EOG6SNDVG.

Enzyme and pathway databases

UniPathwayi UPA00392 .

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiTGT_RICCN
AccessioniPrimary (citable) accession number: Q92GM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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