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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Proton acceptorUniRule annotation1
Binding sitei146SubstrateUniRule annotation1
Binding sitei189SubstrateUniRule annotation1
Binding sitei216Substrate; via amide nitrogenUniRule annotation1
Active sitei266NucleophileUniRule annotation1
Metal bindingi304ZincUniRule annotation1
Metal bindingi306ZincUniRule annotation1
Metal bindingi309ZincUniRule annotation1
Metal bindingi335Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:RC1097
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001355131 – 361Queuine tRNA-ribosyltransferaseAdd BLAST361

Interactioni

Subunit structurei

Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ92GM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 96Substrate bindingUniRule annotation5
Regioni247 – 253RNA bindingUniRule annotation7
Regioni271 – 275RNA binding; important for wobble base 34 recognitionUniRule annotation5

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92GM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFSFNIHH QHKKARSGII VTAHGEMRTP AFMPVGTRGT VKAMLPESVA
60 70 80 90 100
ETGADILLGN TYHLMLQPTA ERIVQLGGLH KFMNWDKPIL TDSGGFQVMS
110 120 130 140 150
LSKLCKITEE GVSFSSHING DKYMLTPERS TEIQYLLGST ITMAFDECTP
160 170 180 190 200
YPATFEEAKT SMQLTTRWAN RSRNAFVKRE GYAQFGIIQG SVYEELREQS
210 220 230 240 250
AKDLVELDFE GYAIGGLAVG EGQELMFKVL DYAPEFLPQN KPRYLMGVGK
260 270 280 290 300
PVDIIGAVSR GIDMFDCVIP TRSGRNGQAF TKYGTVNIRN SKYADDNKPL
310 320 330 340 350
EHDCLCPACR NYSKAYLHHL VRIGEILGSM LMTWHNLTYF QNLMSRIRAY
360
IKLGKDFDFD S
Length:361
Mass (Da):40,590
Last modified:December 1, 2001 - v1
Checksum:i1BEC5C2C39341A84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03635.1.
PIRiA97837.
RefSeqiWP_004997587.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03635; AAL03635; RC1097.
GeneIDi928246.
KEGGirco:RC1097.
PATRICi17889654. VBIRicCon45613_1260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03635.1.
PIRiA97837.
RefSeqiWP_004997587.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92GM6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL03635; AAL03635; RC1097.
GeneIDi928246.
KEGGirco:RC1097.
PATRICi17889654. VBIRicCon45613_1260.

Phylogenomic databases

HOGENOMiHOG000223473.
KOiK00773.
OMAiGIDLFDC.
OrthoDBiPOG091H00XO.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGT_RICCN
AccessioniPrimary (citable) accession number: Q92GM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: December 1, 2001
Last modified: October 5, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.