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Q92GE8

- MNME_RICCN

UniProt

Q92GE8 - MNME_RICCN

Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

    Cofactori

    Binds 1 potassium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201FormyltetrahydrofolateUniRule annotation
    Binding sitei79 – 791FormyltetrahydrofolateUniRule annotation
    Binding sitei119 – 1191FormyltetrahydrofolateUniRule annotation
    Metal bindingi225 – 2251PotassiumUniRule annotation
    Metal bindingi229 – 2291MagnesiumUniRule annotation
    Metal bindingi244 – 2441Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi246 – 2461Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi249 – 2491PotassiumUniRule annotation
    Metal bindingi250 – 2501MagnesiumUniRule annotation
    Binding sitei445 – 4451FormyltetrahydrofolateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi225 – 2306GTPUniRule annotation
    Nucleotide bindingi244 – 2507GTPUniRule annotation
    Nucleotide bindingi269 – 2724GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
    Gene namesi
    Name:mnmEUniRule annotation
    Synonyms:thdFUniRule annotation, trmEUniRule annotation
    Ordered Locus Names:RC1175
    OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
    Taxonomic identifieri272944 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000000816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445tRNA modification GTPase MnmEPRO_0000188909Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi272944.RC1175.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92GE8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini215 – 371157TrmE-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0486.
    HOGENOMiHOG000200714.
    KOiK03650.
    OMAiNIFSSFC.
    OrthoDBiEOG6DC6K1.

    Family and domain databases

    Gene3Di1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00379. GTPase_MnmE.
    InterProiIPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view]
    PfamiPF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS51709. G_TRME. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92GE8-1 [UniParc]FASTAAdd to Basket

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    METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK SRLMYYQQIT    50
    VPETKELIDN VMVVYFKSPG SFTGEDVVEI HTHGSKAISI MLTNALLNIA 100
    GIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH KQAIRQASGK 150
    LEALYNNWRS QLLKMLSLLE AYIDFPDEDI PDTVLNEVTN THTILVNTIS 200
    EYLNDNRKGE LLRSGLKLAI IGPPNVGKSS LLNFLMQRDI AIVSNIAGTT 250
    RDIIEGHLDI GGYPIILQDT AGIREESSDI IEQEGIKRAI NSAKTADIKI 300
    IMFDAEKLDS SINEDIINLI DENTITIINK IDLIEASKIF SIENKYKCLR 350
    VSVKNNIALS SILKNIENIA ENMAGFTETP YITNQRHRNY LQQALSHLTA 400
    FSLDNDLVLA TEDIRMTARC IGAITGVINV EEILGEIFKN FCIGK 445
    Length:445
    Mass (Da):49,400
    Last modified:December 1, 2001 - v1
    Checksum:i58F4BDE2D1CDAE64
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL03713.1.
    PIRiG97846.
    RefSeqiNP_360812.1. NC_003103.1.
    WP_010977743.1. NC_003103.1.

    Genome annotation databases

    EnsemblBacteriaiAAL03713; AAL03713; RC1175.
    GeneIDi928327.
    KEGGirco:RC1175.
    PATRICi17889838. VBIRicCon45613_1351.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL03713.1 .
    PIRi G97846.
    RefSeqi NP_360812.1. NC_003103.1.
    WP_010977743.1. NC_003103.1.

    3D structure databases

    ProteinModelPortali Q92GE8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272944.RC1175.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL03713 ; AAL03713 ; RC1175 .
    GeneIDi 928327.
    KEGGi rco:RC1175.
    PATRICi 17889838. VBIRicCon45613_1351.

    Phylogenomic databases

    eggNOGi COG0486.
    HOGENOMi HOG000200714.
    KOi K03650.
    OMAi NIFSSFC.
    OrthoDBi EOG6DC6K1.

    Family and domain databases

    Gene3Di 1.20.120.430. 3 hits.
    3.30.1360.120. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00379. GTPase_MnmE.
    InterProi IPR018948. GTP-bd_TrmE_N.
    IPR006073. GTP_binding_domain.
    IPR004520. GTPase_MnmE.
    IPR025867. GTPase_MnmE_C_dom.
    IPR027368. MnmE_dom2.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR027266. TrmE/GcvT_dom1.
    [Graphical view ]
    Pfami PF12631. GTPase_Cys_C. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    PF10396. TrmE_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR00450. mnmE_trmE_thdF. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51709. G_TRME. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-613 / Malish 7.

    Entry informationi

    Entry nameiMNME_RICCN
    AccessioniPrimary (citable) accession number: Q92GE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 6, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia conorii
      (strain Malish 7): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3