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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201FormyltetrahydrofolateUniRule annotation
Binding sitei79 – 791FormyltetrahydrofolateUniRule annotation
Binding sitei119 – 1191FormyltetrahydrofolateUniRule annotation
Metal bindingi225 – 2251PotassiumUniRule annotation
Metal bindingi229 – 2291MagnesiumUniRule annotation
Metal bindingi244 – 2441Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi246 – 2461Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi249 – 2491PotassiumUniRule annotation
Metal bindingi250 – 2501MagnesiumUniRule annotation
Binding sitei445 – 4451FormyltetrahydrofolateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi225 – 2306GTPUniRule annotation
Nucleotide bindingi244 – 2507GTPUniRule annotation
Nucleotide bindingi269 – 2724GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.-.-UniRule annotation)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:thdFUniRule annotation, trmEUniRule annotation
Ordered Locus Names:RC1175
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445tRNA modification GTPase MnmEPRO_0000188909Add
BLAST

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi272944.RC1175.

Structurei

3D structure databases

ProteinModelPortaliQ92GE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini215 – 371157TrmE-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0486.
HOGENOMiHOG000200714.
KOiK03650.
OMAiCSGKRLQ.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92GE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK SRLMYYQQIT
60 70 80 90 100
VPETKELIDN VMVVYFKSPG SFTGEDVVEI HTHGSKAISI MLTNALLNIA
110 120 130 140 150
GIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH KQAIRQASGK
160 170 180 190 200
LEALYNNWRS QLLKMLSLLE AYIDFPDEDI PDTVLNEVTN THTILVNTIS
210 220 230 240 250
EYLNDNRKGE LLRSGLKLAI IGPPNVGKSS LLNFLMQRDI AIVSNIAGTT
260 270 280 290 300
RDIIEGHLDI GGYPIILQDT AGIREESSDI IEQEGIKRAI NSAKTADIKI
310 320 330 340 350
IMFDAEKLDS SINEDIINLI DENTITIINK IDLIEASKIF SIENKYKCLR
360 370 380 390 400
VSVKNNIALS SILKNIENIA ENMAGFTETP YITNQRHRNY LQQALSHLTA
410 420 430 440
FSLDNDLVLA TEDIRMTARC IGAITGVINV EEILGEIFKN FCIGK
Length:445
Mass (Da):49,400
Last modified:December 1, 2001 - v1
Checksum:i58F4BDE2D1CDAE64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03713.1.
PIRiG97846.
RefSeqiNP_360812.1. NC_003103.1.
WP_010977743.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03713; AAL03713; RC1175.
GeneIDi928327.
KEGGirco:RC1175.
PATRICi17889838. VBIRicCon45613_1351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03713.1.
PIRiG97846.
RefSeqiNP_360812.1. NC_003103.1.
WP_010977743.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92GE8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272944.RC1175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL03713; AAL03713; RC1175.
GeneIDi928327.
KEGGirco:RC1175.
PATRICi17889838. VBIRicCon45613_1351.

Phylogenomic databases

eggNOGiCOG0486.
HOGENOMiHOG000200714.
KOiK03650.
OMAiCSGKRLQ.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiMNME_RICCN
AccessioniPrimary (citable) accession number: Q92GE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.