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Q92GE8 (MNME_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:thdF, trmE
Ordered Locus Names:RC1175
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP-Rule MF_00379

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00379

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00379.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: HAMAP

GTPase activity

Inferred from electronic annotation. Source: HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445tRNA modification GTPase MnmE HAMAP-Rule MF_00379
PRO_0000188909

Regions

Domain227 – 330104G
Nucleotide binding225 – 2306GTP By similarity
Nucleotide binding244 – 2507GTP By similarity
Nucleotide binding269 – 2724GTP By similarity

Sites

Metal binding2251Potassium By similarity
Metal binding2291Magnesium By similarity
Metal binding2441Potassium; via carbonyl oxygen By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2491Potassium By similarity
Metal binding2501Magnesium By similarity
Binding site201Formyltetrahydrofolate By similarity
Binding site791Formyltetrahydrofolate By similarity
Binding site1191Formyltetrahydrofolate By similarity
Binding site4451Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92GE8 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 58F4BDE2D1CDAE64

FASTA44549,400
        10         20         30         40         50         60 
METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK SRLMYYQQIT VPETKELIDN 

        70         80         90        100        110        120 
VMVVYFKSPG SFTGEDVVEI HTHGSKAISI MLTNALLNIA GIRLAEAGEF TKRAFLNNKF 

       130        140        150        160        170        180 
DLTAAEGIAD LINAETIMQH KQAIRQASGK LEALYNNWRS QLLKMLSLLE AYIDFPDEDI 

       190        200        210        220        230        240 
PDTVLNEVTN THTILVNTIS EYLNDNRKGE LLRSGLKLAI IGPPNVGKSS LLNFLMQRDI 

       250        260        270        280        290        300 
AIVSNIAGTT RDIIEGHLDI GGYPIILQDT AGIREESSDI IEQEGIKRAI NSAKTADIKI 

       310        320        330        340        350        360 
IMFDAEKLDS SINEDIINLI DENTITIINK IDLIEASKIF SIENKYKCLR VSVKNNIALS 

       370        380        390        400        410        420 
SILKNIENIA ENMAGFTETP YITNQRHRNY LQQALSHLTA FSLDNDLVLA TEDIRMTARC 

       430        440 
IGAITGVINV EEILGEIFKN FCIGK

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006914 Genomic DNA. Translation: AAL03713.1.
PIRG97846.
RefSeqNP_360812.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92GE8.
ModBaseSearch...

Protein-protein interaction databases

STRING272944.RC1175.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03713; AAL03713; RC1175.
GeneID928327.
KEGGrco:RC1175.
PATRIC17889838. VBIRicCon45613_1351.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0486.
HOGENOMHOG000200714.
KOK03650.
OMAPGEFSEQ.
ProtClustDBPRK05291.

Family and domain databases

Gene3D1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
HAMAPMF_00379. GTPase_MnmE.
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR025867. GTPase_MnmE_C_dom.
IPR027368. MnmE_dom2.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamPF12631. GTPase_Cys_C. 1 hit.
PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_RICCN
AccessionPrimary (citable) accession number: Q92GE8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: December 1, 2001
Last modified: May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia conorii

(strain Malish 7): entries and gene names

SIMILARITY comments

Index of protein domains and families

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