Reviewed, UniProtKB/Swiss-Prot Q92G90 (ACON_RICCN)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:	acnA
Ordered Locus Names:	RC1233

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Aconitate hydratase		PRO_0000076664
Sites
	Metal binding	426	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	492	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	495	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92G90-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 1065AB1CF4E1822D	FASTA	878	96,900
	10 20 30 40 50 60 MSKVHNSEYI QELSVDNTSY KIYDINKAAS DIDLPLKKLP YSLRVLFENV LRSNGSKQSL 70 80 90 100 110 120 LVFKEWLKTK KSDAEIDFMP ARVLMQDFTG VPAIVDLAAM RDAMKKIGGD PLKINPLIPV 130 140 150 160 170 180 DLVIDHSVSV DSYAAKDSFN KNVQMEMKRN IERYAFLKWG QQAFNNFKVV PPGTGICHQV 190 200 210 220 230 240 NLEYLAKVVW HKDGTLYPDS LVGTDSHTTM VNGLSVLGWG VGGIEAEAAM LGQPLTMILP 250 260 270 280 290 300 EVIGVKLTGK LTGIATATDL VLTVTEMLRK KKVVGKFVEF FGEGLKNLMI ADRATISNMS 310 320 330 340 350 360 PEYGATCGFF PIDQETIKYL ELTGREKTQI RLVEQYATEQ NLWYDFEHAV EYTEVLELDL 370 380 390 400 410 420 SMVHGSLAGP KRPQDRVNLN DVASNFKYEL PNFALDNKDI DKKYAVANQN YEIGNGDVVI 430 440 450 460 470 480 AAITSCTNTS NPSVMIGAAL LAKKALEHGL KVKPWVKTSL APGSKVVTEY LKLSGLDKYL 490 500 510 520 530 540 DELGFNLVGY GCTTCIGNSG PLNPEIEETI NKNGLVVASV LSGNRNFEGR INPLTKASYL 550 560 570 580 590 600 GSPILVVAYA LSGTLNIDLT NMPIGENIYL KDLWPSKEEI DEVIANSINS SMFIEKYSDI 610 620 630 640 650 660 FSGTKEWKDL QVTNSSTYNW NKNSTYINNP PYFKDIGSKN NIQDIKSAKI LAILGDSITT 670 680 690 700 710 720 DHISPAGSIS KTSPAAKYLT DHHIEPLDFN SYGSRRGNHE VMMRGTFANI RIKNEMCKGV 730 740 750 760 770 780 EGGFTINQLN GTQQTIYDAA MDYKAHDVSV VIFAGKEYGS GSSRDWAAKG PGLLGVKAVI 790 800 810 820 830 840 AESFERIHRS NLVGMGILPL TFTGNNTRLD LKLDGSETID ITGLSENISS YHPVKCVIKK 850 860 870 QTGAIRTIDL ILQIFTDNEI NYIKHGSIMH FVVESLKG

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL03771.1.
PIR	A97854.
RefSeq	NP_360870.1.
3D structure databases
SMR	Q92G90. Positions 9-876.
ModBase	Search...
Genome annotation databases
GeneID	928381.
GenomeReviews	Gene locus RC1233 in contig AE006914_GR.
KEGG	rco:RC1233.
NMPDR	fig|272944.1.peg.1233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MLYPDSV.
Enzyme and pathway databases
BioCyc	RCON272944:RC1233-MONOMER.
BRENDA	4.2.1.3. 267518.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_RICCN

Accession

Primary (citable) accession number: Q92G90

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)