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Q92G13 (RFTRM_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional methyltransferase

Including the following 2 domains:

  1. Release factor glutamine methyltransferase
    Short name=RF MTase
    EC=2.1.1.297
    Alternative name(s):
    M.RcoHemKP
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
  2. tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
Gene names
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RC1314
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity. HAMAP-Rule MF_01057

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine. HAMAP-Rule MF_01057

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Sequence similarities

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Bifunctional methyltransferase HAMAP-Rule MF_01057
PRO_0000157173

Regions

Region1 – 308308RF MTase HAMAP-Rule MF_01057
Region1 – 306306HemK HAMAP-Rule MF_01057
Region146 – 1505S-adenosyl-L-methionine binding By similarity
Region213 – 2164Substrate binding By similarity
Region307 – 524218tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
Region311 – 524214tRNA MTase HAMAP-Rule MF_01057

Sites

Active site4271 By similarity
Binding site1691S-adenosyl-L-methionine By similarity
Binding site1981S-adenosyl-L-methionine By similarity
Binding site2131S-adenosyl-L-methionine By similarity
Binding site3531S-adenosyl-L-methionine By similarity
Binding site3781S-adenosyl-L-methionine By similarity
Binding site4051S-adenosyl-L-methionine By similarity
Binding site4271S-adenosyl-L-methionine By similarity
Binding site4311Substrate By similarity
Binding site4631Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92G13 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 3E574D6CF7DFDC28

FASTA52460,079
        10         20         30         40         50         60 
MQCSIKQILS DATDKLNKIG ISSPQLEARI LLQHVINKPI EYLLINLDEQ LNEAKIEAFE 

        70         80         90        100        110        120 
KLLARRLKHE PIVYITGVKE FYSREFIVNK HVLIPRSDTE VLVDVVFQCH SRESGNPEKK 

       130        140        150        160        170        180 
QPNPCFRGND ISENCNDKFL NILELGTGSG CIAISLLCEL PNANVIATDI SLDAIDIIKS 

       190        200        210        220        230        240 
NAAKYEVTDR IQIIHSNWFE NIETQKFDFI VSNPPYIAHS EKSEMAIETI NYEPSIALFA 

       250        260        270        280        290        300 
EKDGLQAYFL IAENAKQFLK PNGKIILEIG FKQEEAVTQI FLDHGYNIES VYKDLQGHSR 

       310        320        330        340        350        360 
VILFSPINLN RSYARRIGKS LSGVQKNLLD NKLPKYLFSK EKLVNEKRKV FLEIGFGMGE 

       370        380        390        400        410        420 
HFINQAKMNP NALFIGIEVY LNGVANVLKL AGEQNITNFL LFPNNLDLIL NEIPSNSLDG 

       430        440        450        460        470        480 
IYILFPDPWI KNKQKKKRIF NKERLKLLQD KLKDNGNLVF ASDIENYFYE AIELIQQNGN 

       490        500        510        520 
FEIINKNDYL KPHDNYVITK YHQKAIKANR TPKFMILRHV LGDH 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL03852.1.
PIRB97864.
RefSeqNP_360951.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92G13.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC1314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL03852; AAL03852; RC1314.
GeneID928460.
KEGGrco:RC1314.
PATRIC17890159. VBIRicCon45613_1508.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000010896.
KOK02493.
OMAQQNLLDN.
OrthoDBEOG68Q0SZ.
ProtClustDBPRK01544.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFTRM_RICCN
AccessionPrimary (citable) accession number: Q92G13
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names