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Q92G13

- RFTRM_RICCN

UniProt

Q92G13 - RFTRM_RICCN

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Protein
Bifunctional methyltransferase
Gene
prmC/trmB, hemK, RC1314
Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity.UniRule annotation
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691S-adenosyl-L-methionine By similarity
Binding sitei198 – 1981S-adenosyl-L-methionine By similarity
Binding sitei213 – 2131S-adenosyl-L-methionine By similarity
Binding sitei353 – 3531S-adenosyl-L-methionine By similarity
Binding sitei378 – 3781S-adenosyl-L-methionine By similarity
Binding sitei405 – 4051S-adenosyl-L-methionine By similarity
Active sitei427 – 4271 By similarity
Binding sitei427 – 4271S-adenosyl-L-methionine By similarity
Binding sitei431 – 4311Substrate By similarity
Binding sitei463 – 4631Substrate By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional methyltransferase
    Including the following 2 domains:
    Release factor glutamine methyltransferase (EC:2.1.1.297)
    Short name:
    RF MTase
    Alternative name(s):
    M.RcoHemKP
    N5-glutamine methyltransferase PrmC
    Protein-(glutamine-N5) MTase PrmC
    Protein-glutamine N-methyltransferase PrmC
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:prmC/trmB
    Synonyms:hemK
    Ordered Locus Names:RC1314
    OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
    Taxonomic identifieri272944 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000000816: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524Bifunctional methyltransferaseUniRule annotation
    PRO_0000157173Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272944.RC1314.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92G13.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 308308RF MTaseUniRule annotation
    Add
    BLAST
    Regioni1 – 306306HemKUniRule annotation
    Add
    BLAST
    Regioni146 – 1505S-adenosyl-L-methionine binding By similarity
    Regioni213 – 2164Substrate binding By similarity
    Regioni307 – 524218tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    Add
    BLAST
    Regioni311 – 524214tRNA MTaseUniRule annotation
    Add
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
    In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000010896.
    KOiK02493.
    OMAiQQNLLDN.
    OrthoDBiEOG68Q0SZ.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92G13-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQCSIKQILS DATDKLNKIG ISSPQLEARI LLQHVINKPI EYLLINLDEQ    50
    LNEAKIEAFE KLLARRLKHE PIVYITGVKE FYSREFIVNK HVLIPRSDTE 100
    VLVDVVFQCH SRESGNPEKK QPNPCFRGND ISENCNDKFL NILELGTGSG 150
    CIAISLLCEL PNANVIATDI SLDAIDIIKS NAAKYEVTDR IQIIHSNWFE 200
    NIETQKFDFI VSNPPYIAHS EKSEMAIETI NYEPSIALFA EKDGLQAYFL 250
    IAENAKQFLK PNGKIILEIG FKQEEAVTQI FLDHGYNIES VYKDLQGHSR 300
    VILFSPINLN RSYARRIGKS LSGVQKNLLD NKLPKYLFSK EKLVNEKRKV 350
    FLEIGFGMGE HFINQAKMNP NALFIGIEVY LNGVANVLKL AGEQNITNFL 400
    LFPNNLDLIL NEIPSNSLDG IYILFPDPWI KNKQKKKRIF NKERLKLLQD 450
    KLKDNGNLVF ASDIENYFYE AIELIQQNGN FEIINKNDYL KPHDNYVITK 500
    YHQKAIKANR TPKFMILRHV LGDH 524
    Length:524
    Mass (Da):60,079
    Last modified:December 1, 2001 - v1
    Checksum:i3E574D6CF7DFDC28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL03852.1.
    PIRiB97864.
    RefSeqiNP_360951.1. NC_003103.1.

    Genome annotation databases

    EnsemblBacteriaiAAL03852; AAL03852; RC1314.
    GeneIDi928460.
    KEGGirco:RC1314.
    PATRICi17890159. VBIRicCon45613_1508.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL03852.1 .
    PIRi B97864.
    RefSeqi NP_360951.1. NC_003103.1.

    3D structure databases

    ProteinModelPortali Q92G13.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 272944.RC1314.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL03852 ; AAL03852 ; RC1314 .
    GeneIDi 928460.
    KEGGi rco:RC1314.
    PATRICi 17890159. VBIRicCon45613_1508.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000010896.
    KOi K02493.
    OMAi QQNLLDN.
    OrthoDBi EOG68Q0SZ.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    MF_02126. RF_methyltr_PrmC.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR004556. Modification_methylase_HemK.
    IPR019874. Release_fac_Glu-N5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    IPR007848. Small_mtfrase_dom.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    PF05175. MTS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
    TIGR03534. RF_mod_PrmC. 1 hit.
    TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-613 / Malish 7.

    Entry informationi

    Entry nameiRFTRM_RICCN
    AccessioniPrimary (citable) accession number: Q92G13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: December 1, 2001
    Last modified: June 11, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia conorii
      (strain Malish 7): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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