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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei169S-adenosyl-L-methionineBy similarity1
Binding sitei198S-adenosyl-L-methionineBy similarity1
Binding sitei213S-adenosyl-L-methionineBy similarity1
Binding sitei353S-adenosyl-L-methionineBy similarity1
Binding sitei378S-adenosyl-L-methionineBy similarity1
Binding sitei405S-adenosyl-L-methionineBy similarity1
Active sitei427By similarity1
Binding sitei427S-adenosyl-L-methionineBy similarity1
Binding sitei431SubstrateBy similarity1
Binding sitei463SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
M.RcoHemKP
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RC1314
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001571731 – 524Bifunctional methyltransferaseAdd BLAST524

Proteomic databases

PRIDEiQ92G13

Structurei

3D structure databases

ProteinModelPortaliQ92G13
SMRiQ92G13
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 308RF MTaseAdd BLAST308
Regioni1 – 306HemKAdd BLAST306
Regioni146 – 150S-adenosyl-L-methionine bindingBy similarity5
Regioni213 – 216Substrate bindingBy similarity4
Regioni307 – 524tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST218
Regioni311 – 524tRNA MTaseAdd BLAST214

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000010896
KOiK02493
OMAiDDISENC
OrthoDBiPOG091H02CJ

Family and domain databases

HAMAPiMF_02126 RF_methyltr_PrmC, 1 hit
MF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR004556 Modification_methylase_HemK
IPR019874 Release_fac_Glu-N5_MeTfrase
IPR029063 SAM-dependent_MTases
IPR007848 Small_mtfrase_dom
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PfamiView protein in Pfam
PF05175 MTS, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
TIGRFAMsiTIGR00536 hemK_fam, 1 hit
TIGR03534 RF_mod_PrmC, 1 hit
TIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q92G13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCSIKQILS DATDKLNKIG ISSPQLEARI LLQHVINKPI EYLLINLDEQ
60 70 80 90 100
LNEAKIEAFE KLLARRLKHE PIVYITGVKE FYSREFIVNK HVLIPRSDTE
110 120 130 140 150
VLVDVVFQCH SRESGNPEKK QPNPCFRGND ISENCNDKFL NILELGTGSG
160 170 180 190 200
CIAISLLCEL PNANVIATDI SLDAIDIIKS NAAKYEVTDR IQIIHSNWFE
210 220 230 240 250
NIETQKFDFI VSNPPYIAHS EKSEMAIETI NYEPSIALFA EKDGLQAYFL
260 270 280 290 300
IAENAKQFLK PNGKIILEIG FKQEEAVTQI FLDHGYNIES VYKDLQGHSR
310 320 330 340 350
VILFSPINLN RSYARRIGKS LSGVQKNLLD NKLPKYLFSK EKLVNEKRKV
360 370 380 390 400
FLEIGFGMGE HFINQAKMNP NALFIGIEVY LNGVANVLKL AGEQNITNFL
410 420 430 440 450
LFPNNLDLIL NEIPSNSLDG IYILFPDPWI KNKQKKKRIF NKERLKLLQD
460 470 480 490 500
KLKDNGNLVF ASDIENYFYE AIELIQQNGN FEIINKNDYL KPHDNYVITK
510 520
YHQKAIKANR TPKFMILRHV LGDH
Length:524
Mass (Da):60,079
Last modified:December 1, 2001 - v1
Checksum:i3E574D6CF7DFDC28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA Translation: AAL03852.1
PIRiB97864
RefSeqiWP_010977871.1, NC_003103.1

Genome annotation databases

EnsemblBacteriaiAAL03852; AAL03852; RC1314
GeneIDi928460
KEGGirco:RC1314

Entry informationi

Entry nameiRFTRM_RICCN
AccessioniPrimary (citable) accession number: Q92G13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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