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Q92G13

- RFTRM_RICCN

UniProt

Q92G13 - RFTRM_RICCN

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Protein

Bifunctional methyltransferase

Gene
prmC/trmB, hemK, RC1314
Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif By similarity.UniRule annotation
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.UniRule annotation
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691S-adenosyl-L-methionine By similarity
Binding sitei198 – 1981S-adenosyl-L-methionine By similarity
Binding sitei213 – 2131S-adenosyl-L-methionine By similarity
Binding sitei353 – 3531S-adenosyl-L-methionine By similarity
Binding sitei378 – 3781S-adenosyl-L-methionine By similarity
Binding sitei405 – 4051S-adenosyl-L-methionine By similarity
Active sitei427 – 4271 By similarity
Binding sitei427 – 4271S-adenosyl-L-methionine By similarity
Binding sitei431 – 4311Substrate By similarity
Binding sitei463 – 4631Substrate By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. protein methyltransferase activity Source: InterPro
  3. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
M.RcoHemKP
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RC1314
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Bifunctional methyltransferaseUniRule annotationPRO_0000157173Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272944.RC1314.

Structurei

3D structure databases

ProteinModelPortaliQ92G13.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 308308RF MTaseUniRule annotationAdd
BLAST
Regioni1 – 306306HemKUniRule annotationAdd
BLAST
Regioni146 – 1505S-adenosyl-L-methionine binding By similarity
Regioni213 – 2164Substrate binding By similarity
Regioni307 – 524218tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationAdd
BLAST
Regioni311 – 524214tRNA MTaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiQQNLLDN.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92G13-1 [UniParc]FASTAAdd to Basket

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MQCSIKQILS DATDKLNKIG ISSPQLEARI LLQHVINKPI EYLLINLDEQ    50
LNEAKIEAFE KLLARRLKHE PIVYITGVKE FYSREFIVNK HVLIPRSDTE 100
VLVDVVFQCH SRESGNPEKK QPNPCFRGND ISENCNDKFL NILELGTGSG 150
CIAISLLCEL PNANVIATDI SLDAIDIIKS NAAKYEVTDR IQIIHSNWFE 200
NIETQKFDFI VSNPPYIAHS EKSEMAIETI NYEPSIALFA EKDGLQAYFL 250
IAENAKQFLK PNGKIILEIG FKQEEAVTQI FLDHGYNIES VYKDLQGHSR 300
VILFSPINLN RSYARRIGKS LSGVQKNLLD NKLPKYLFSK EKLVNEKRKV 350
FLEIGFGMGE HFINQAKMNP NALFIGIEVY LNGVANVLKL AGEQNITNFL 400
LFPNNLDLIL NEIPSNSLDG IYILFPDPWI KNKQKKKRIF NKERLKLLQD 450
KLKDNGNLVF ASDIENYFYE AIELIQQNGN FEIINKNDYL KPHDNYVITK 500
YHQKAIKANR TPKFMILRHV LGDH 524
Length:524
Mass (Da):60,079
Last modified:December 1, 2001 - v1
Checksum:i3E574D6CF7DFDC28
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03852.1.
PIRiB97864.
RefSeqiNP_360951.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03852; AAL03852; RC1314.
GeneIDi928460.
KEGGirco:RC1314.
PATRICi17890159. VBIRicCon45613_1508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03852.1 .
PIRi B97864.
RefSeqi NP_360951.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92G13.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272944.RC1314.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL03852 ; AAL03852 ; RC1314 .
GeneIDi 928460.
KEGGi rco:RC1314.
PATRICi 17890159. VBIRicCon45613_1508.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000010896.
KOi K02493.
OMAi QQNLLDN.
OrthoDBi EOG68Q0SZ.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiRFTRM_RICCN
AccessioniPrimary (citable) accession number: Q92G13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi