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Protein

Bifunctional methyltransferase

Gene

prmC/trmB

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.By similarity
Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

Catalytic activityi

S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine.
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei169 – 1691S-adenosyl-L-methionineBy similarity
Binding sitei198 – 1981S-adenosyl-L-methionineBy similarity
Binding sitei213 – 2131S-adenosyl-L-methionineBy similarity
Binding sitei353 – 3531S-adenosyl-L-methionineBy similarity
Binding sitei378 – 3781S-adenosyl-L-methionineBy similarity
Binding sitei405 – 4051S-adenosyl-L-methionineBy similarity
Active sitei427 – 4271By similarity
Binding sitei427 – 4271S-adenosyl-L-methionineBy similarity
Binding sitei431 – 4311SubstrateBy similarity
Binding sitei463 – 4631SubstrateBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional methyltransferase
Including the following 2 domains:
Release factor glutamine methyltransferase (EC:2.1.1.297)
Short name:
RF MTase
Alternative name(s):
M.RcoHemKP
N5-glutamine methyltransferase PrmC
Protein-(glutamine-N5) MTase PrmC
Protein-glutamine N-methyltransferase PrmC
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:prmC/trmB
Synonyms:hemK
Ordered Locus Names:RC1314
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Bifunctional methyltransferasePRO_0000157173Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ92G13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 308308RF MTaseAdd
BLAST
Regioni1 – 306306HemKAdd
BLAST
Regioni146 – 1505S-adenosyl-L-methionine bindingBy similarity
Regioni213 – 2164Substrate bindingBy similarity
Regioni307 – 524218tRNA (guanine-N(7)-)-methyltransferaseAdd
BLAST
Regioni311 – 524214tRNA MTaseAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.Curated
In the N-terminal section; belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.Curated

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiKSNAIKH.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92G13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCSIKQILS DATDKLNKIG ISSPQLEARI LLQHVINKPI EYLLINLDEQ
60 70 80 90 100
LNEAKIEAFE KLLARRLKHE PIVYITGVKE FYSREFIVNK HVLIPRSDTE
110 120 130 140 150
VLVDVVFQCH SRESGNPEKK QPNPCFRGND ISENCNDKFL NILELGTGSG
160 170 180 190 200
CIAISLLCEL PNANVIATDI SLDAIDIIKS NAAKYEVTDR IQIIHSNWFE
210 220 230 240 250
NIETQKFDFI VSNPPYIAHS EKSEMAIETI NYEPSIALFA EKDGLQAYFL
260 270 280 290 300
IAENAKQFLK PNGKIILEIG FKQEEAVTQI FLDHGYNIES VYKDLQGHSR
310 320 330 340 350
VILFSPINLN RSYARRIGKS LSGVQKNLLD NKLPKYLFSK EKLVNEKRKV
360 370 380 390 400
FLEIGFGMGE HFINQAKMNP NALFIGIEVY LNGVANVLKL AGEQNITNFL
410 420 430 440 450
LFPNNLDLIL NEIPSNSLDG IYILFPDPWI KNKQKKKRIF NKERLKLLQD
460 470 480 490 500
KLKDNGNLVF ASDIENYFYE AIELIQQNGN FEIINKNDYL KPHDNYVITK
510 520
YHQKAIKANR TPKFMILRHV LGDH
Length:524
Mass (Da):60,079
Last modified:December 1, 2001 - v1
Checksum:i3E574D6CF7DFDC28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03852.1.
PIRiB97864.
RefSeqiWP_010977871.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03852; AAL03852; RC1314.
GeneIDi928460.
KEGGirco:RC1314.
PATRICi17890159. VBIRicCon45613_1508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL03852.1.
PIRiB97864.
RefSeqiWP_010977871.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92G13.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL03852; AAL03852; RC1314.
GeneIDi928460.
KEGGirco:RC1314.
PATRICi17890159. VBIRicCon45613_1508.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000010896.
KOiK02493.
OMAiKSNAIKH.
OrthoDBiEOG68Q0SZ.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
MF_02126. RF_methyltr_PrmC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR004556. Modification_methylase_HemK.
IPR019874. Release_fac_Glu-N5_MeTfrase.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
PF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00536. hemK_fam. 1 hit.
TIGR03534. RF_mod_PrmC. 1 hit.
TIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiRFTRM_RICCN
AccessioniPrimary (citable) accession number: Q92G13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: December 1, 2001
Last modified: July 22, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.