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Q92G00

- SYA_RICCN

UniProt

Q92G00 - SYA_RICCN

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Protein

Alanine--tRNA ligase

Gene
alaS, RC1327
Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671Zinc Reviewed prediction
Metal bindingi571 – 5711Zinc Reviewed prediction
Metal bindingi669 – 6691Zinc Reviewed prediction
Metal bindingi673 – 6731Zinc Reviewed prediction

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:RC1327
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Alanine--tRNA ligaseUniRule annotationPRO_0000075190Add
BLAST

Proteomic databases

PRIDEiQ92G00.

Interactioni

Protein-protein interaction databases

STRINGi272944.RC1327.

Structurei

3D structure databases

ProteinModelPortaliQ92G00.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiYDIDIFQ.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92G00-1 [UniParc]FASTAAdd to Basket

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MTKFTTEEVR SKFITYFKAN NHTHVPASSL IPHNDPSLMF VNSGMVQFKN    50
VFTGQGKRPY NKAVTSQKSL RAGGKHNDLE NVGYTARHHT FFEMLGNFSF 100
GDYFKEQAIY YAWNLLTKEF ELPKDKLYAT IYHTDDEAAA YWKKIAGFGD 150
DRIIKIKTND NFWSMGDTGP CGPCSEIFYD HGEQIYGGLP GTKDEDGDRF 200
IEIWNMVFMQ YEQIDKDTSI ELSQKSIDTG MGLERMTAVL QHVNNNYDID 250
LFQEIINFTE NIVKVKVEGE AKFSYRVIAD HLRASSFLIA DGVIPSNEGR 300
GYVLRRIMRR SMRHAHMLGS KEPLMYKLLP KLVDLMGNVY PELKRAESFI 350
SSILEQEEIR FKATLERGLK LLTEETETLT KGNELSGEVA FKLYDTYGFP 400
LDLTEDILKN RDIAVDHKGF EEQMLMQKAR ARKSWLGSGE SKTDQLWFDI 450
KEQHGSTEFL GYTLNEAKCK IIALIKNNNL VNDIKEIDTQ FLLISNQTPF 500
YGESGGQIGD IGTIFAKDSE VEVIDTLKYL GSIIIHKCIL KKGQINVGEN 550
ANFSIDIRYR QNLRIHHSAT HILHAVLHEV LGKHVTQKGS LVAPTYLRFD 600
ISHSKAVTNE EITLIEDKVN EIIRDNHEVT TTLMATEDAI KQGAMALFGE 650
KYDSEVRVVK MGETSLELCG GTHVRRTGDI GCFKITSESA IAAGVRRIEA 700
VCGEFVITLM REKDSLLKSI ESSFKTNKNE LITKVNNILE RNKEVEKELE 750
KTLLASLDLS IEQIEKQSAQ ITGIKLLYKK VGNIDNKILR QAAENLTKKV 800
EDLIMVYIAE GIGKLSITVA VSKAITDKYN ADIIAKKLSL FLGGSGGGGQ 850
ASLAQAGGND IGKLTNIHEK LYSLLTVS 878
Length:878
Mass (Da):98,838
Last modified:December 1, 2001 - v1
Checksum:iF4E6A0A13379E65A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03865.1.
PIRiG97865.
RefSeqiNP_360964.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL03865; AAL03865; RC1327.
GeneIDi928521.
KEGGirco:RC1327.
PATRICi17890185. VBIRicCon45613_1521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL03865.1 .
PIRi G97865.
RefSeqi NP_360964.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92G00.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272944.RC1327.

Proteomic databases

PRIDEi Q92G00.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL03865 ; AAL03865 ; RC1327 .
GeneIDi 928521.
KEGGi rco:RC1327.
PATRICi 17890185. VBIRicCon45613_1521.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi YDIDIFQ.
OrthoDBi EOG6Q2SQ2.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiSYA_RICCN
AccessioniPrimary (citable) accession number: Q92G00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi