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Q92F90 (SYM_LISIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Synonyms:metS
Ordered Locus Names:lin0216
OrganismListeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]
Taxonomic identifier272626 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 664664Methionine--tRNA ligase HAMAP-Rule MF_01228
PRO_0000139225

Regions

Domain563 – 664102tRNA-binding
Motif15 – 2511"HIGH" region HAMAP-Rule MF_01228
Motif310 – 3145"KMSKS" region HAMAP-Rule MF_01228

Sites

Binding site3131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92F90 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 64A526B817BB8195

FASTA66475,756
        10         20         30         40         50         60 
MPEEKNTFYI TTPIYYPSGK AHIGHAYTTV AGDAMARYKR LKGYDVFYLT GTDEHGQKIQ 

        70         80         90        100        110        120 
AKAKERGISE QEYVDEIAEG FQELWKKLEI SNTDFIRTTQ DRHKTSVEKI FEQLLEQGDI 

       130        140        150        160        170        180 
YLGEYEGWYS VSDEEYFTET QLEEVYKDEN GKVIGGKAPS GNEVELVKEE SYFFRMSKYA 

       190        200        210        220        230        240 
DRLVEYYNSH PEFILPESRK NEMINNFIKP GLEDLAVSRT TFDWGIKVPG NPKHVVYVWI 

       250        260        270        280        290        300 
DALSNYITAL GYNTDNDTKF QKYWPADVQI VGKEIVRFHT IYWPIMLMAL DLPLPKMVFG 

       310        320        330        340        350        360 
HGWILMKDGK MSKSKGNVVD PYMLIDRYGL DALRYYLLRE VPFGSDGLFT PEDFVDRVNY 

       370        380        390        400        410        420 
DLANDLGNLL NRTVAMINKY FDGEIPAYQG NVTEFDQTLV DFKNNVVKEY EGSMDHMQFS 

       430        440        450        460        470        480 
VALNQLWSLI SRTNKYIDET APWALAKEED KRTELASVMT HLAENLRIIA VLLQPFLTRT 

       490        500        510        520        530        540 
PGEIFLQLGL QEENLKKWDS IYGYGEIPEG TTVVKKGTPI FPRLDAEVEV TYIQDEMKGS 

       550        560        570        580        590        600 
APAPAEETAE VEALETPQIG IEDFDKIDLR VAEVKQVDKV KKADKLLCFQ LDLGEGKLRQ 

       610        620        630        640        650        660 
VLSGIAEFYQ PEELIGKKVI VVSNLKPVKL RGLMSEGMIL SGEKDGKLSV IEANSALPNG 


AKVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL596163 Genomic DNA. Translation: CAC95449.1.
PIRAI1459.
RefSeqNP_469561.1. NC_003212.1.

3D structure databases

ProteinModelPortalQ92F90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272626.lin0216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC95449; CAC95449; CAC95449.
GeneID1128620.
KEGGlin:lin0216.
PATRIC20297076. VBILisInn102668_0227.

Organism-specific databases

GenoListLIN0216.
CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200401.
KOK01874.
OMAANEWGNL.
OrthoDBEOG6CVV9B.
ProtClustDBPRK12267.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_LISIN
AccessionPrimary (citable) accession number: Q92F90
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries