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Q92F56

- TILS_LISIN

UniProt

Q92F56 - TILS_LISIN

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Protein
Bifunctional protein TilS/HprT
Gene
tilS/hprT, lin0251
Organism
Listeria innocua serovar 6a (strain CLIP 11262)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.UniRule annotation

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Cofactori

Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi627 – 6271Magnesium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. purine ribonucleoside salvage Source: InterPro
  2. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Ligase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:tilS/hprT
Ordered Locus Names:lin0251
OrganismiListeria innocua serovar 6a (strain CLIP 11262)
Taxonomic identifieri272626 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002513: Chromosome

Organism-specific databases

GenoListiLIN0251.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Bifunctional protein TilS/HprTUniRule annotation
PRO_0000181716Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272626.lin0251.

Structurei

3D structure databases

ProteinModelPortaliQ92F56.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000217053.
KOiK15780.
OMAiNDISYPL.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92F56-1 [UniParc]FASTAAdd to Basket

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MDDTKKRIHK YIEKHDLIRS DDKLLVAVSG GPDSFALLHF LWSANLVPKE    50
AIAVAHLNHH LRENAEKEQL AVQTFCEERD IPFFIEEVDV KKRAEKLQKG 100
IEETARIVRY DFFEKVMAEN NINKLVLAHH ADDQIETILM RLVRGSSSIG 150
WSGIQPKREV RGGYAIRPFL PITKAEIIEY ATKHALPYEI DESNTSQEYT 200
RNRYRTQLLP FLSKENPAVY EHFKRFSEET SEDFQFLEEL ASNLLKKNLI 250
QNGKQSTLLL SDFKNEANPL QRRAIHLLLM YLYNDDGRVI TVNHIYQIIQ 300
MIQSENPSSS IDLPKKLTVI RSYNELHFQF GERHAPPEFY HQLELNDRIE 350
LDNKASIRLK LKSSVVQTNG LNGMLLDAED ITLPLIVRNR VNGDRMTMKG 400
QAGSKKLKDI FIDAKIPRQE RDNLPVITDY TGKILWVPGV KKSAYDREFS 450
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTAEYE 500
GRNPLVVGVL KGATPFMTDL LKRIDTYLEM DFMDVSSYGN GTVSSGEVKI 550
IKDLNASVEG RDVLIIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP 600
AGRNVAIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE 648
Length:648
Mass (Da):74,527
Last modified:December 1, 2001 - v1
Checksum:i796860D8E403E0CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL596164 Genomic DNA. Translation: CAC95484.1.
PIRiAD1464.
RefSeqiNP_469596.1. NC_003212.1.
WP_010990297.1. NC_003212.1.

Genome annotation databases

EnsemblBacteriaiCAC95484; CAC95484; CAC95484.
GeneIDi1128659.
KEGGilin:lin0251.
PATRICi20297148. VBILisInn102668_0263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL596164 Genomic DNA. Translation: CAC95484.1 .
PIRi AD1464.
RefSeqi NP_469596.1. NC_003212.1.
WP_010990297.1. NC_003212.1.

3D structure databases

ProteinModelPortali Q92F56.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272626.lin0251.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC95484 ; CAC95484 ; CAC95484 .
GeneIDi 1128659.
KEGGi lin:lin0251.
PATRICi 20297148. VBILisInn102668_0263.

Organism-specific databases

GenoListi LIN0251.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000217053.
KOi K15780.
OMAi NDISYPL.
OrthoDBi EOG693GNP.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_01161. tRNA_Ile_lys_synt.
InterProi IPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view ]
PANTHERi PTHR11807:SF2. PTHR11807:SF2. 1 hit.
Pfami PF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view ]
SMARTi SM00977. TilS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIP 11262.

Entry informationi

Entry nameiTILS_LISIN
AccessioniPrimary (citable) accession number: Q92F56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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