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Q92F56

- TILS_LISIN

UniProt

Q92F56 - TILS_LISIN

Protein

Bifunctional protein TilS/HprT

Gene

tilS/hprT

Organism
Listeria innocua serovar 6a (strain CLIP 11262)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.
    (tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.

    Cofactori

    Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi627 – 6271Magnesium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 346ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. purine ribonucleoside salvage Source: InterPro
    2. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Ligase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein TilS/HprT
    Including the following 2 domains:
    tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
    Alternative name(s):
    tRNA(Ile)-2-lysyl-cytidine synthase
    tRNA(Ile)-lysidine synthetase
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:tilS/hprT
    Ordered Locus Names:lin0251
    OrganismiListeria innocua serovar 6a (strain CLIP 11262)
    Taxonomic identifieri272626 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000002513: Chromosome

    Organism-specific databases

    GenoListiLIN0251.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648Bifunctional protein TilS/HprTPRO_0000181716Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272626.lin0251.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92F56.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

    Sequence similaritiesi

    In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.Curated
    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000217053.
    KOiK15780.
    OMAiNDISYPL.
    OrthoDBiEOG693GNP.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_01161. tRNA_Ile_lys_synt.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR012094. Lysidine-tRNA-synth.
    IPR012796. Lysidine-tRNA-synth_C.
    IPR012795. Lysidine-tRNA-synth_N.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view]
    PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
    PfamiPF01171. ATP_bind_3. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    PF11734. TilS_C. 1 hit.
    [Graphical view]
    SMARTiSM00977. TilS_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    TIGR02433. lysidine_TilS_C. 1 hit.
    TIGR02432. lysidine_TilS_N. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92F56-1 [UniParc]FASTAAdd to Basket

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    MDDTKKRIHK YIEKHDLIRS DDKLLVAVSG GPDSFALLHF LWSANLVPKE    50
    AIAVAHLNHH LRENAEKEQL AVQTFCEERD IPFFIEEVDV KKRAEKLQKG 100
    IEETARIVRY DFFEKVMAEN NINKLVLAHH ADDQIETILM RLVRGSSSIG 150
    WSGIQPKREV RGGYAIRPFL PITKAEIIEY ATKHALPYEI DESNTSQEYT 200
    RNRYRTQLLP FLSKENPAVY EHFKRFSEET SEDFQFLEEL ASNLLKKNLI 250
    QNGKQSTLLL SDFKNEANPL QRRAIHLLLM YLYNDDGRVI TVNHIYQIIQ 300
    MIQSENPSSS IDLPKKLTVI RSYNELHFQF GERHAPPEFY HQLELNDRIE 350
    LDNKASIRLK LKSSVVQTNG LNGMLLDAED ITLPLIVRNR VNGDRMTMKG 400
    QAGSKKLKDI FIDAKIPRQE RDNLPVITDY TGKILWVPGV KKSAYDREFS 450
    RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTAEYE 500
    GRNPLVVGVL KGATPFMTDL LKRIDTYLEM DFMDVSSYGN GTVSSGEVKI 550
    IKDLNASVEG RDVLIIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP 600
    AGRNVAIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE 648
    Length:648
    Mass (Da):74,527
    Last modified:December 1, 2001 - v1
    Checksum:i796860D8E403E0CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL596164 Genomic DNA. Translation: CAC95484.1.
    PIRiAD1464.
    RefSeqiNP_469596.1. NC_003212.1.
    WP_010990297.1. NC_003212.1.

    Genome annotation databases

    EnsemblBacteriaiCAC95484; CAC95484; CAC95484.
    GeneIDi1128659.
    KEGGilin:lin0251.
    PATRICi20297148. VBILisInn102668_0263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL596164 Genomic DNA. Translation: CAC95484.1 .
    PIRi AD1464.
    RefSeqi NP_469596.1. NC_003212.1.
    WP_010990297.1. NC_003212.1.

    3D structure databases

    ProteinModelPortali Q92F56.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272626.lin0251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC95484 ; CAC95484 ; CAC95484 .
    GeneIDi 1128659.
    KEGGi lin:lin0251.
    PATRICi 20297148. VBILisInn102668_0263.

    Organism-specific databases

    GenoListi LIN0251.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000217053.
    KOi K15780.
    OMAi NDISYPL.
    OrthoDBi EOG693GNP.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_01161. tRNA_Ile_lys_synt.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR012094. Lysidine-tRNA-synth.
    IPR012796. Lysidine-tRNA-synth_C.
    IPR012795. Lysidine-tRNA-synth_N.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view ]
    PANTHERi PTHR11807:SF2. PTHR11807:SF2. 1 hit.
    Pfami PF01171. ATP_bind_3. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    PF11734. TilS_C. 1 hit.
    [Graphical view ]
    SMARTi SM00977. TilS_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    TIGR02433. lysidine_TilS_C. 1 hit.
    TIGR02432. lysidine_TilS_N. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CLIP 11262.

    Entry informationi

    Entry nameiTILS_LISIN
    AccessioniPrimary (citable) accession number: Q92F56
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3