Q92CL7 (COBB_LISIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase

Gene names

Name:	cobB
Synonyms:	cbiA
Ordered Locus Names:	lin1154

Organism

Listeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]

Taxonomic identifier

272626 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria innocua

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. Contains 1 GATase cobBQ-type domain.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Domain	Glutamine amidotransferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027		PRO_0000141259
Regions
	Domain	248 – 441	194	GATase cobBQ-type
Sites
	Active site	330	1	Nucleophile By similarity
	Active site	433	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92CL7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: B0F7F07F2D7FDDD1	FASTA	452	49,762
	10 20 30 40 50 60 MNKILIAAAS SGAGKTTVTL GIMQALKKRG FAVQPFKVGP DYIDTNYHQA ITGVASINLD 70 80 90 100 110 120 SFLIDDDEML ASLFQKHGES ADISVIEGVM GLFDGLGTDR DNASTSFIAK CTKTPVILVV 130 140 150 160 170 180 DGKAISTSAA AIVDGFNRFD PELKIAGVII NRVASENHFS LIKGAIERYT DVPVLGYLPK 190 200 210 220 230 240 NAAVALPERH LGLVPQEEMT ELEAKWELLS DLITTHVDLD KLLEISKSSE NLSTSKAQMK 250 260 270 280 290 300 VADFSGLRVA YALDAAFHFY YQDNLDLIRL TGAELIPFSP LEDNEVPEAD FIYIGGGFPE 310 320 330 340 350 360 IFAKQLNDNR SMRKSILAAH EKGIPIYAEC GGLMYLGSSL EMEGKQYEMV GVFNGISKMT 370 380 390 400 410 420 TRLRKFGYCI AEPLEETLIG KKGMSIRGHE FHHSVFETTE TACMKLSKKR DGEIVKEWRG 430 440 450 GYQKGNTFAS YLHIHFYQNP AILMQMFGAG KR

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL596167 Genomic DNA. Translation: CAC96385.1.
PIR	AI1576.
RefSeq	NP_470491.1. NC_003212.1.
3D structure databases
ProteinModelPortal	Q92CL7.
ModBase	Search...
Protein-protein interaction databases
STRING	272626.lin1154.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC96385; CAC96385; CAC96385.
GeneID	1129704.
KEGG	lin:lin1154.
PATRIC	20299029. VBILisInn102668_1182.
Organism-specific databases
GenoList	LIN1154.
CMR	Search...
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289958.
KO	K02224.
OMA	CWLPGGY.
ProtClustDB	PRK01077.
Enzyme and pathway databases
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COBB_LISIN

Accession

Primary (citable) accession number: Q92CL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program