Q92CI7 (SYFA_LISIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	lin1184

Organism

Listeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]

Taxonomic identifier

272626 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria innocua

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_0000126723
Sites
	Metal binding	257	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92CI7 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: E22F859D73CED543	FASTA	350	39,200
	10 20 30 40 50 60 MLEQLQTLKS EAETQINEAA DLKTLNDLRV KYLGKKGPMT EIMKQMGKLS AEERPKMGSL 70 80 90 100 110 120 ANEVRTALTE AISSKQEILE TAAINEKLKS ETIDVTLPGT APSIGTKHLL TQVIEEMEDM 130 140 150 160 170 180 FIGMGYEIAE GPEVELDYYN FEALNLPKDH PARDMQDSFY ITENTLLRTQ TSPVQARTME 190 200 210 220 230 240 KHDFSKGPIK VICPGKVYRR DNDDATHSHQ FTQIEGLVVG ENITFADLKG TLTVLAKTMF 250 260 270 280 290 300 GEEREIRLRP SFFPFTEPSV EMDISCFKCG GKGCRVCKGT GWIEILGSGM VHPNVLEMSG 310 320 330 340 350 IDSTRYSGFA FGLGPERVAM LKYAVDDIRH LYTNDLRFTK QFQSTETGEI

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL596167 Genomic DNA. Translation: CAC96415.1.
PIR	AG1580.
RefSeq	NP_470521.1. NC_003212.1.
3D structure databases
ProteinModelPortal	Q92CI7.
SMR	Q92CI7. Positions 84-346.
ModBase	Search...
Protein-protein interaction databases
STRING	272626.lin1184.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC96415; CAC96415; CAC96415.
GeneID	1129741.
KEGG	lin:lin1184.
PATRIC	20299089. VBILisInn102668_1212.
Organism-specific databases
GenoList	LIN1184.
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HOG000242675.
KO	K01889.
OMA	LTHTPMF.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_LISIN

Accession

Primary (citable) accession number: Q92CI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program