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Q92C77 (Q92C77_LISIN) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL CAC96545.1
Ordered Locus Names:lin1314
OrganismListeria innocua [Complete proteome] [HAMAP]
Taxonomic identifier1642 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q92C77 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5549B1CF45D397F1

FASTA69279,378
        10         20         30         40         50         60 
MADYLVIVES PAKAKTIEKY LGKKFKVKAS MGHVRDLPKS QMGVDTEHDY EPRYITIRGK 

        70         80         90        100        110        120 
GPVLKDLKQA AKKAKKVYLA ADPDREGEAI AWHLANSLDL DQSDKLRVVF NEITKEAVKE 

       130        140        150        160        170        180 
SFKTPRKIDM DLVNAQQARR ILDRLVGYNI SPILWKKVKK GLSAGRVQSI ALRIIIDREK 

       190        200        210        220        230        240 
EINNFKPEEY WTIDGNFLKG KKKFQANFYG VNGKKRKLST ADDVKEVMSA IKGKTFDVTD 

       250        260        270        280        290        300 
VTKKERLRNP AAPFTTSSLQ QEAARKLNYR TRKTMMLAQQ LYEGITLGKQ GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRIADSA ILEASNYIKE TYGAEFSRNH KRSDKNAKGA QDAHEAIRPT SAMRSPQEVK 

       370        380        390        400        410        420 
EYLTRDQLRL YRLIWERFIA SQMTPAVLDT MRVDLDNNGV NFRANGSKIK FHGFMKVYVE 

       430        440        450        460        470        480 
SNDDNTEEKE NILPDLKKGD KVQSESLEQR QHFTQPPPRY TEARLVKTLE EIGIGRPSTY 

       490        500        510        520        530        540 
SPTLDTIQRR NYVSLTNKRF VPTELGEIVN EMIEEYFPEI LDVKFTANME SELDEVEHGE 

       550        560        570        580        590        600 
VEWVKVIDEF YKQFEPNVIK ADAEMEKIEI KDEPAGIDCD LCGAPMVYKM GKYGKFLACS 

       610        620        630        640        650        660 
RFPDCRNTKA IVKEIGVTCP KCEKGHVIER KSKKKRIFYG CDRYPDCDYV SWDKPVERAC 

       670        680        690 
PKCNKRALVE KKLKKGVQVQ CTNCDYKEST QQ

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL596168 Genomic DNA. Translation: CAC96545.1.
PIRAI1596.
RefSeqNP_470650.1. NC_003212.1.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ92C77.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1129902.
GenomeReviewsGene locus lin1314 in contig AL592022_GR.
KEGGlin:lin1314.
NMPDRfig|272626.1.peg.1303.
PATRIC20299359. VBILisInn102668_1346.

Organism-specific databases

GenoListLIN1314.
CMRSearch...

Phylogenomic databases

HOGENOMHBG512935.
OMAIERNIRH.
ProtClustDBPRK05582.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ92C77_LISIN
AccessionPrimary (citable) accession number: Q92C77
Entry history
Integrated into UniProtKB/TrEMBL: December 1, 2001
Last sequence update: December 1, 2001
Last modified: December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info