Reviewed, UniProtKB/Swiss-Prot Q92BI0 (LDH2_LISIN)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	lin1569

Organism

Listeria innocua [Complete proteome] [HAMAP]

Taxonomic identifier

1642 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	L-lactate dehydrogenase 2		PRO_0000168362
Regions
	Nucleotide binding	12 – 40	29	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	122	1	NAD or substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92BI0-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F953C292AEA87E10	FASTA	311	33,854
	10 20 30 40 50 60 MKPRKVMIIG AGNVGSAAAH AFVNQKFVEE LILVDLNKER VEGNRKDLAD AAAFMSGKMD 70 80 90 100 110 120 ISVREASDCA DVDIAVITVT AGPLKEGQTR LDELRSTSRI VASIVPEMMK GGFKGIFLIA 130 140 150 160 170 180 TNPCDIITYQ VWKLSGLPRE QVLGTGVWLD TTRLRRLLAE KLDIAAQSID AFILGEHGDS 190 200 210 220 230 240 QFPVWSHSSI YGKPVNEYSM EKLGESLDLK LIGETARDTG FEIYHQKGCT EYGIAGTIVE 250 260 270 280 290 300 ICRHIFSGSQ RALTVSCVLD GEYGQTGLAI GVPAVLSQNG VKEIISLKLN EQEQQAFDHS 310 AAVIKENIKS I

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262 / Serovar 6a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL596169 Genomic DNA. Translation: CAC96800.1.
PIR	AH1628.
RefSeq	NP_470905.1.
3D structure databases
HSSP	HSSP built from PDB template 1LDB based on UniProtKB P00344.
ModBase	Search...
Genome annotation databases
GeneID	1130186.
GenomeReviews	Gene locus lin1569 in contig AL592022_GR.
KEGG	lin:lin1569.
NMPDR	fig|272626.1.peg.1558.
Organism-specific databases
ListiList	LIN01569.
CMR	Search...
Phylogenomic databases
HOGENOM	Q92BI0.
Enzyme and pathway databases
BioCyc	LINN272626:LIN1569-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LISIN

Accession

Primary (citable) accession number: Q92BI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)