Q92AP4 (PUR2_LISIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	lin1876

Organism

Listeria innocua [Complete proteome] [HAMAP]

Taxonomic identifier

1642 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151460
Regions
	Domain	108 – 314	207	ATP-grasp
	Nucleotide binding	134 – 195	62	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92AP4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DACFD3493C1D3C5A	FASTA	420	45,672
	10 20 30 40 50 60 MNLLVVGSGG REHAISKKLL ESNNVEKVYC APGNDGMRLD DIQLVAISET DKVGLIDFAK 70 80 90 100 110 120 KADVSFVIVG PEVPLLEGVV DAFEEAGIKA FGPKANAALI EGSKDFAKQF MEKYAIPTAA 130 140 150 160 170 180 SRTFTDYAEA KAYLDERGVP IVIKADGLAA GKGVTVALEM EEAVLALKDM MLEEKFGDAS 190 200 210 220 230 240 LKVVIEDFLA GEEFSLMAFV NGEEVYPMAI AQDHKRAYEG DKGPNTGGMG AYSPVPHISE 250 260 270 280 290 300 DVVNEAVEKI LRPAAKGMVQ EDRYFRGILY AGLILTTEGP KVIEFNARFG DPETQVVLPR 310 320 330 340 350 360 LESDFAALIA ALLNNEKPEV RFKKEGITLG VVLASAGYPE HYEKGNKLTG LNDIREDVAI 370 380 390 400 410 420 YHAGTKQNEN GEFVSSGGRV LLLAKEAETM SDARTLLYPE MQKLDNPNFF YRMDIGTKAE

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262 / Serovar 6a.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL596170 Genomic DNA. Translation: CAC97106.1.
PIR	AB1667.
RefSeq	NP_471210.1. NC_003212.1.
3D structure databases
ProteinModelPortal	Q92AP4.
SMR	Q92AP4. Positions 1-419.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1130556.
GenomeReviews	Gene locus lin1876 in contig AL592022_GR.
KEGG	lin:lin1876.
NMPDR	fig|272626.1.peg.1863.
PATRIC	20300557. VBILisInn102668_1920.
Organism-specific databases
GenoList	LIN1876.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404060.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	LINN272626:LIN1876-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LISIN

Accession

Primary (citable) accession number: Q92AP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program