ID   PYRC_LISIN              Reviewed;         426 AA.
AC   Q92AH1;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Dihydroorotase;
DE            Short=DHOase;
DE            EC=3.5.2.3;
GN   Name=pyrC; OrderedLocusNames=lin1951;
OS   Listeria innocua.
OC   Bacteria; Firmicutes; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=1642;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIP 11262 / Serovar 6a;
RX   MEDLINE=21537279; PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A.,
RA   Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T.,
RA   Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P.,
RA   Domann E., Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O.,
RA   Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P.,
RA   Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D.,
RA   Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G.,
RA   Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H.,
RA   Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R.,
RA   Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A.,
RA   Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 3/6.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
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DR   EMBL; AL596170; CAC97181.1; -; Genomic_DNA.
DR   PIR; AE1676; AE1676.
DR   RefSeq; NP_471285.1; -.
DR   HSSP; P81006; 1GKR.
DR   MEROPS; M38.972; -.
DR   GeneID; 1130647; -.
DR   GenomeReviews; AL592022_GR; lin1951.
DR   KEGG; lin:lin1951; -.
DR   NMPDR; fig|272626.1.peg.1938; -.
DR   ListiList; LIN01951; -.
DR   HOGENOM; Q92AH1; -.
DR   OMA; Q92AH1; GIFAEKE.
DR   BioCyc; LINN272626:LIN1951-MON; -.
DR   BRENDA; 3.5.2.3; 270396.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00220; -; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOmult.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   ProDom; PD000518; DHOase; 1.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    426       Dihydroorotase.
FT                                /FTId=PRO_0000147239.
FT   METAL        58     58       Zinc 1 (By similarity).
FT   METAL        60     60       Zinc 1 (By similarity).
FT   METAL       140    140       Zinc 1; via carbamate group (By
FT                                similarity).
FT   METAL       140    140       Zinc 2; via carbamate group (By
FT                                similarity).
FT   METAL       177    177       Zinc 2 (By similarity).
FT   METAL       230    230       Zinc 2 (By similarity).
FT   METAL       303    303       Zinc 1 (By similarity).
FT   MOD_RES     140    140       N6-carboxylysine (By similarity).
SQ   SEQUENCE   426 AA;  46201 MW;  D8591D40D111FED3 CRC64;
     MYVLKNGQVL NESGELENKD VLIQNGKVNL IADSIEVTSG EEFDATGKLI APGFIDVHVH
     LREPGGEHKE TILTGTKAAA RGGYTTICSM PNTKPVPDSK EVMNSLQAKI KETAEVRVLP
     YASITTSLGT DELVDFEALK EAGAFAFTDD GVGVQLAGTM YEAMKRAAAL DMAIVAHCED
     NSLIYGGVVH DGIFAEKEGL KGIPNIAESV QIARDVLLAE AAGCHYHVCH ISTKESVRVV
     RDAKRAGIRV TAEVSPHHLI LDEEDIPGND GNWKMNPPLR SKEDRAALLE GLLDGTIDFI
     ATDHAPHAAE EKNVPMEQAA FGIVGLETAF PLLYTHFVKT NEWTLKQLID WMTVKPAECF
     KLPYGKLEEG AVADIVVLDL EKEATIDPAT FYSKGKNTPF VGETCIGWPV ATFAEGELVY
     NEGENK
//