Reviewed, UniProtKB/Swiss-Prot Q929Z2 (NADB_LISIN)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	lin2131

Organism

Listeria innocua [Complete proteome] [HAMAP]

Taxonomic identifier

1642 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	L-aspartate oxidase		PRO_0000184386
Regions
	Nucleotide binding	7 – 21	15	FAD Potential
Sites
	Active site	224	1	By similarity
	Active site	243	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q929Z2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 548B9BA10EBA4261	FASTA	484	53,411
	10 20 30 40 50 60 MTKERVIIIG SGIAGCTAAL RLMQDYDVTI ITKGHKEQSN SMLAQGGVAA AISKSDTPKK 70 80 90 100 110 120 HFSDTFQAGC FHNKVRAVNQ LVTCGPIVIQ KLINEGMTFD EKDGELSLGL EGAHQLPRIL 130 140 150 160 170 180 HSGGDQTGKF LTTFLQEKLT KVNWQEQKMA IEIIKHDGRA IGVHCLDKEN QLHTYYGEHI 190 200 210 220 230 240 ILASGGLGQL FPVTTNAATI SGDGLALAYR ADATLSDMEF IQFHPTLLFL NGRCHGLISE 250 260 270 280 290 300 AVRGEGATLI RADGSAIMET VHPLADLAPR DVVAATLFEE IERGNQVFLD ITKVAHFEKR 310 320 330 340 350 360 FPAITTSLDE HQVPFRETKR IPVHPGAHFL MGGVRTDLNG KTNIPHLYAI GEVANTGVHG 370 380 390 400 410 420 ANRLASNSLL ETLVFGEKVA EYIHTQKNNR IIAPENPPTY QTTMPHLPEK HILQEKIWET 430 440 450 460 470 480 LGITRKPEKI TEFLHWLETF DYANHTRETA EISHMIITSK LIAESALKRT ESLGAHRILK GVTK

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262 / Serovar 6a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL596171 Genomic DNA. Translation: CAC97361.1.
PIR	AI1698.
RefSeq	NP_471465.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1130858.
GenomeReviews	Gene locus lin2131 in contig AL592022_GR.
KEGG	lin:lin2131.
NMPDR	fig|272626.1.peg.2118.
Organism-specific databases
ListiList	LIN02131.
CMR	Search...
Phylogenomic databases
HOGENOM	Q929Z2.
OMA	GAYIWNR.
Enzyme and pathway databases
BioCyc	LINN272626:LIN2131-MON.
BRENDA	1.4.3.16. 270396.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_LISIN

Accession

Primary (citable) accession number: Q929Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)