Reviewed, UniProtKB/Swiss-Prot Q929E8 (FUMC_LISIN)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Synonyms:	citG
Ordered Locus Names:	lin2328

Organism

Listeria innocua [Complete proteome] [HAMAP]

Taxonomic identifier

1642 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161284
Regions
	Region	122 – 125	4	B site By similarity
	Region	132 – 134	3	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q929E8-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 800F577E7E5BEB38	FASTA	455	49,737
	10 20 30 40 50 60 MERIERDTLG EISVDATKYW GAQTERSRRN FAIGDNYMPK EIIYAFAQLK KAAAKVNALE 70 80 90 100 110 120 GKLSEAKSVA IGNVCDQIIQ GELDEHFPLV VWQTGSGTQS NMNVNEVIAH VANLTLGEEK 130 140 150 160 170 180 VHPNDDVNMS QSSNDTFPTA MHIAAYQALV TKLLSEITKM EEVLTTKKNQ YMQLVKIGRT 190 200 210 220 230 240 HLQDATPLTL GQEISGWEAC LTNNKNYLES SMKAILPLAI GGTAVGTGLN ASKDFGDKVA 250 260 270 280 290 300 EELRKQTGYP FTSDSNKYFA LTSHSPINFV HGAIRSLASD LMKIANDIRL LASGPRSGIG 310 320 330 340 350 360 ELTLPANEPG SSIMPGKVNP TQCEAITMVA AQVMGNDTTI NIAASQGNFE LNVYKPVIIF 370 380 390 400 410 420 NFLESITLLS DSMRSFRIHC LEGLTANESV IEAKVNDSLM LVTALNPHIG YEKAAKIAKL 430 440 450 AFDENTTLKE AAIKTGFVTE KEFDLWIDPL KMTNL

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262 / Serovar 6a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL596171 Genomic DNA. Translation: CAC97556.1.
PIR	AD1723.
RefSeq	NP_471660.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
ModBase	Search...
Genome annotation databases
GeneID	1131082.
GenomeReviews	Gene locus lin2328 in contig AL592022_GR.
KEGG	lin:lin2328.
NMPDR	fig|272626.1.peg.2313.
Organism-specific databases
ListiList	LIN02328.
CMR	Search...
Phylogenomic databases
HOGENOM	Q929E8.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	LINN272626:LIN2328-MON.
BRENDA	4.2.1.2. 270396.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_LISIN

Accession

Primary (citable) accession number: Q929E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)