Q92997 (DVL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Segment polarity protein dishevelled homolog DVL-3
Short name=Dishevelled-3

Alternative name(s):
DSH homolog 3

Gene names

Name:	DVL3
Synonyms:	KIAA0208

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the signal transduction pathway mediated by multiple Wnt genes.
Subunit structure	Interacts (via the PDZ domain) with the C-terminal regions of VANGL1 and VANGL2 By similarity. Interacts (via the region containing both the PDZ and DEP domains) with LRRFIP2; the DIX domain may inhibit this interaction. Interacts with CYLD. Ref.7 Ref.9
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Ref.9 Phosphorylated by CSNK1D. Ref.8 Ref.10
Sequence similarities	Belongs to the DSH family. Contains 1 DEP domain. Contains 1 DIX domain. Contains 1 PDZ (DHR) domain.
Sequence caution	The sequence BAA13199.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Developmental protein
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	canonical Wnt receptor signaling pathway Inferred from direct assay. Source: BHF-UCL cochlea morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome convergent extension Inferred from Biological aspect of Ancestor. Source: RefGenome intracellular signal transduction Inferred from electronic annotation. Source: InterPro outflow tract septum morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome planar cell polarity pathway involved in neural tube closure Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of JUN kinase activity Inferred from mutant phenotype. Source: BHF-UCL positive regulation of protein phosphorylation Inferred from mutant phenotype. Source: BHF-UCL positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: BHF-UCL
Cellular component	cell cortex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	beta-catenin binding Inferred from direct assay. Source: BHF-UCL frizzled binding Inferred from physical interaction. Source: UniProtKB protease binding Inferred from physical interaction Ref.9. Source: UniProtKB protein heterodimerization activity Inferred from direct assay. Source: BHF-UCL signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LRRFIP2	Q9Y608	2	EBI-739789,EBI-1023718
PRPF3	O43395	2	EBI-739789,EBI-744322

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Segment polarity protein dishevelled homolog DVL-3		PRO_0000145749
Regions
	Domain	1 – 82	82	DIX
	Domain	249 – 321	73	PDZ
	Domain	422 – 496	75	DEP
Amino acid modifications
	Modified residue	346	1	Phosphothreonine Ref.8
Natural variations
	Natural variant	216	1	R → T in a breast cancer sample; somatic mutation. Ref.11		VAR_036116
	Natural variant	433	1	W → L. Ref.6 Corresponds to variant rs17853048 [ dbSNP | Ensembl ].		VAR_025519
Experimental info
	Sequence conflict	2	1	G → D in AAB47447. Ref.1
	Sequence conflict	76	1	S → Y in AAB84228. Ref.2
	Sequence conflict	102	1	P → S in AAB47447. Ref.1
	Sequence conflict	151	1	G → W in AAB47447. Ref.1
	Sequence conflict	182	1	S → R in AAB47447. Ref.1
	Sequence conflict	218	1	K → N in AAB47447. Ref.1
	Sequence conflict	222	1	R → W in AAB47447. Ref.1
	Sequence conflict	230	1	E → D in AAB47447. Ref.1
	Sequence conflict	233	1	S → C in AAB47447. Ref.1
	Sequence conflict	236	1	S → T in AAB47447. Ref.1
	Sequence conflict	239	1	T → S in AAB47447. Ref.1
	Sequence conflict	242	1	T → A in AAB47447. Ref.1
	Sequence conflict	303	1	N → K in AAB47447. Ref.1
	Sequence conflict	431	1	R → C in AAB47447. Ref.1
	Sequence conflict	450	1	W → C in AAB47447. Ref.1
	Sequence conflict	465	1	R → P in AAB47447. Ref.1
	Sequence conflict	472	1	L → V in AAB47447. Ref.1
	Sequence conflict	543	1	P → R in AAB65244. Ref.3
	Sequence conflict	553 – 554	2	FP → LG in AAB65244. Ref.3
	Sequence conflict	629	1	A → T in AAB47447. Ref.1
	Sequence conflict	633	1	S → I in AAB47447. Ref.1
	Sequence conflict	682 – 716	35	PPGRD…FVDVM → LRAATWPQCPRN in BAA13199. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q92997 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: B1A55EBF9507D06E	FASTA	716	78,055
	10 20 30 40 50 60 MGETKIIYHL DGQETPYLVK LPLPAERVTL ADFKGVLQRP SYKFFFKSMD DDFGVVKEEI 70 80 90 100 110 120 SDDNAKLPCF NGRVVSWLVS AEGSHPDPAP FCADNPSELP PPMERTGGIG DSRPPSFHPH 130 140 150 160 170 180 AGGGSQENLD NDTETDSLVS AQRERPRRRD GPEHATRLNG TAKGERRREP GGYDSSSTLM 190 200 210 220 230 240 SSELETTSFF DSDEDDSTSR FSSSTEQSSA SRLMRRHKRR RRKQKVSRIE RSSSFSSITD 250 260 270 280 290 300 STMSLNIITV TLNMEKYNFL GISIVGQSNE RGDGGIYIGS IMKGGAVAAD GRIEPGDMLL 310 320 330 340 350 360 QVNEINFENM SNDDAVRVLR EIVHKPGPIT LTVAKCWDPS PRGCFTLPRS EPIRPIDPAA 370 380 390 400 410 420 WVSHTAAMTG TFPAYGMSPS LSTITSTSSS ITSSIPDTER LDDFHLSIHS DMAAIVKAMA 430 440 450 460 470 480 SPESGLEVRD RMWLKITIPN AFIGSDVVDW LYHNVEGFTD RREARKYASN LLKAGFIRHT 490 500 510 520 530 540 VNKITFSEQC YYIFGDLCGN MANLSLHDHD GSSGASDQDT LAPLPHPGAA PWPMAFPYQY 550 560 570 580 590 600 PPPPHPYNPH PGFPELGYSY GGGSASSQHS EGSRSSGSNR SGSDRRKEKD PKAGDSKSGG 610 620 630 640 650 660 SGSESDHTTR SSLRGPRERA PSERSGPAAS EHSHRSHHSL ASSLRSHHTH PSYGPPGVPP 670 680 690 700 710 LYGPPMLMMP PPPAAMGPPG APPGRDLASV PPELTASRQS FRMAMGNPSE FFVDVM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA characterization and chromosomal mapping of two human homologues of the Drosophila dishevelled polarity gene." Pizzuti A., Amati F., Calabrese G., Mari A., Colosimo A., Silani V., Giardino L., Ratti A., Penso D., Calza L., Palka G., Scarlato G., Novelli G., Dallapiccola B. Hum. Mol. Genet. 5:953-958(1996) [PubMed: 8817329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"cDNA cloning of a human dishevelled DVL-3 gene, mapping to 3q27, and expression in human breast and colon carcinomas." Bui T.D., Beier D.R., Jonssen M., Smith K., Dorrington S.M., Kaklamanis L., Kearney L., Regan R., Sussman D.J., Harris A.L. Biochem. Biophys. Res. Commun. 239:510-516(1997) [PubMed: 9344861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Human dishevelled genes constitute a DHR-containing multigene family." Semenov M.V., Snyder M. Genomics 42:302-310(1997) [PubMed: 9192851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed: 9039502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-433. Tissue: Brain.
[7]	"Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis." Liu J., Bang A.G., Kintner C., Orth A.P., Chanda S.K., Ding S., Schultz P.G. Proc. Natl. Acad. Sci. U.S.A. 102:1927-1932(2005) [PubMed: 15677333] [Abstract] Cited for: INTERACTION WITH LRRFIP2.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-346, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Loss of the tumor suppressor CYLD enhances Wnt/beta-catenin signaling through K63-linked ubiquitination of Dvl." Tauriello D.V., Haegebarth A., Kuper I., Edelmann M.J., Henraat M., Canninga-van Dijk M.R., Kessler B.M., Clevers H., Maurice M.M. Mol. Cell 37:607-619(2010) [PubMed: 20227366] [Abstract] Cited for: UBIQUITINATION, DEUBIQUITINATION, INTERACTION WITH CYLD.
[10]	"Casein kinase 1 delta functions at the centrosome to mediate Wnt-3a-dependent neurite outgrowth." Greer Y.E., Rubin J.S. J. Cell Biol. 192:993-1004(2011) [PubMed: 21422228] [Abstract] Cited for: PHOSPHORYLATION BY CSNK1D/CK1.
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-216.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49262 mRNA. Translation: AAB47447.1. U75651 mRNA. Translation: AAB84228.1. AF006013 mRNA. Translation: AAB65244.1. D86963 mRNA. Translation: BAA13199.2. Different initiation. CH471052 Genomic DNA. Translation: EAW78295.1. CH471052 Genomic DNA. Translation: EAW78296.1. BC032459 mRNA. Translation: AAH32459.1.
IPI	IPI00643141.
PIR	JC5763.
RefSeq	NP_004414.3. NM_004423.3.
UniGene	Hs.388116.
3D structure databases
ProteinModelPortal	Q92997.
SMR	Q92997. Positions 3-79, 247-335, 411-499.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92997. 30 interactions.
MINT	MINT-1186858.
STRING	Q92997.
PTM databases
PhosphoSite	Q92997.
Polymorphism databases
DMDM	6919875.
Proteomic databases
PRIDE	Q92997.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000313143; ENSP00000316054; ENSG00000161202.
GeneID	1857.
KEGG	hsa:1857.
UCSC	uc003fms.1. human.
Organism-specific databases
CTD	1857.
GeneCards	GC03P183873.
H-InvDB	HIX0003920.
HGNC	HGNC:3087. DVL3.
MIM	601368. gene.
neXtProt	NX_Q92997.
PharmGKB	PA27543.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13158.
GeneTree	ENSGT00390000013552.
HOGENOM	HBG717800.
HOVERGEN	HBG005542.
InParanoid	Q92997.
OMA	IHSDMAT.
OrthoDB	EOG4TQM8S.
PhylomeDB	Q92997.
Gene expression databases
ArrayExpress	Q92997.
Bgee	Q92997.
CleanEx	HS_DVL3.
Genevestigator	Q92997.
GermOnline	ENSG00000161202. Homo sapiens.
Family and domain databases
InterPro	IPR000591. DEP_dom. IPR008339. Dishevelled. IPR008342. Dishevelled_3. IPR024580. Dishevelled_C-dom. IPR003351. Dishevelled_protein_dom. IPR001158. DIX. IPR015506. Dsh. IPR001478. PDZ/DHR/GLGF. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K02353.
PANTHER	PTHR10878. Dsh. 1 hit.
Pfam	PF00610. DEP. 1 hit. PF02377. Dishevelled. 1 hit. PF00778. DIX. 1 hit. PF12316. Dsh_C. 1 hit. PF00595. PDZ. 1 hit. [Graphical view]
PRINTS	PR01760. DISHEVELLED. PR01763. DISHEVELLED3.
SMART	SM00021. DAX. 1 hit. SM00049. DEP. 1 hit. SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50186. DEP. 1 hit. PS50841. DIX. 1 hit. PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	7609.
SOURCE	Search...

Entry information

Entry name

DVL3_HUMAN

Accession

Primary (citable) accession number: Q92997
Secondary accession number(s): D3DNT0 , O14642, Q13531, Q8N5E9, Q92607

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.