Reviewed, UniProtKB/Swiss-Prot Q92995 (UBP13_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 13
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 13
    Ubiquitin-specific-processing protease 13
    Deubiquitinating enzyme 13
    Isopeptidase T-3
      Short name=ISOT-3

Gene names

Name:	USP13
Synonyms:	ISOT3

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.
Tissue specificity	Highly expressed in ovary and testes.
Sequence similarities	Belongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Traceable author statement. Source: ProtInc ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	Ubiquitin carboxyl-terminal hydrolase 13		PRO_0000080635
Regions
	Domain	652 – 693	42	UBA 1
	Domain	727 – 767	41	UBA 2
	Zinc finger	209 – 281	73	UBP-type
Sites
	Active site	345	1	By similarity
	Active site	814	1	By similarity
	Active site	823	1	By similarity
Amino acid modifications
	Modified residue	114	1	Phosphoserine Ref.6
	Modified residue	122	1	Phosphothreonine Ref.6 Ref.4
Experimental info
	Sequence conflict	96	1	V → A in AAC63405. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q92995-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 988713ADF8C0B938	FASTA	863	97,327
	10 20 30 40 50 60 MQRRGALFGM PGGSGGRKMA AGDIGELLVP HMPTIRVPRS GDRVYKNECA FSYDSPNSEG 70 80 90 100 110 120 GLYVCMNTFL AFGREHVERH FRKTGQSVYM HLKRHVREKV RGASGGALPK RRNSKIFLDL 130 140 150 160 170 180 DTDDDLNSDD YEYEDEAKLV IFPDHYEIAL PNIEELPALV TIACDAVLSS KSPYRKQDPD 190 200 210 220 230 240 TWENELPVSK YANNLTQLDN GVRIPPSGWK CARCDLRENL WLNLTDGSVL CGKWFFDSSG 250 260 270 280 290 300 GNGHALEHYR DMGYPLAVKL GTITPDGADV YSFQEEEPVL DPHLAKHLAH FGIDMLHMHG 310 320 330 340 350 360 TENGLQDNDI KLRVSEWEVI QESGTKLKPM YGPGYTGLKN LGNSCYLSSV MQAIFSIPEF 370 380 390 400 410 420 QRAYVGNLPR IFDYSPLDPT QDFNTQMTKL GHGLLSGQYS KPPVKSELIE QVMKEEHKPQ 430 440 450 460 470 480 QNGISPRMFK AFVSKSHPEF SSNRQQDAQE FFLHLVNLVE RNRIGSENPS DVFRFLVEER 490 500 510 520 530 540 IQCCQTRKVR YTERVDYLMQ LPVAMEAATN KDELIAYELT RREAEANRRP LPELVRAKIP 550 560 570 580 590 600 FSACLQAFSE PENVDDFWSS ALQAKSAGVK TSRFASFPEY LVVQIKKFTF GLDWVPKKFD 610 620 630 640 650 660 VSIDMPDLLD INHLRARGLQ PGEEELPDIS PPIVIPDDSK DRLMNQLIDP SDIDESSVMQ 670 680 690 700 710 720 LAEMGFPLEA CRKAVYFTGN MGAEVAFNWI IVHMEEPDFA EPLTMPGYGG AASAGASVFG 730 740 750 760 770 780 ASGLDNQPPE EIVAIITSMG FQRNQAIQAL RATNNNLERA LDWIFSHPEF EEDSDFVIEM 790 800 810 820 830 840 ENNANANIIS EAKPEGPRVK DGSGTYELFA FISHMGTSTM SGHYICHIKK EGRWVIYNDH 850 860 KVCASERPPK DLGYMYFYRR IPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genomic organization of Isopeptidase T-3 (ISOT-3), a new member of the ubiquitin specific protease family (UBP)." Timms K.M., Ansari-Lari M.A., Morris W., Brown S.N., Gibbs R.A. Gene 217:101-106(1998) [PubMed: 9841226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, MASS SPECTROMETRY.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND THR-122, MASS SPECTROMETRY. Tissue: T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U75362 mRNA. Translation: AAC63405.1. CH471052 Genomic DNA. Translation: EAW78383.1. BC016146 mRNA. Translation: AAH16146.1.
IPI	IPI00024401.
RefSeq	NP_003931.2.
UniGene	Hs.175322
3D structure databases
SMR	Q92995. Positions 188-301, 331-595, 616-709, 686-769.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92995. 7 interactions.
STRING	Q92995.
Protein family/group databases
MEROPS	C19.012.
PTM databases
PhosphoSite	Q92995.
Proteomic databases
PRIDE	Q92995.
Genome annotation databases
Ensembl	ENST00000263966; ENSP00000263966; ENSG00000058056; Homo sapiens. [Genome view]
GeneID	8975.
KEGG	hsa:8975.
UCSC	uc003fkg.1. human.
Organism-specific databases
CTD	8975.
GeneCards	GC03P180853.
H-InvDB	HIX0003883.
HGNC	HGNC:12611. USP13.
HPA	HPA004827.
MIM	603591. gene.
PharmGKB	PA134939640.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17331.
HOGENOM	HBG746888.
HOVERGEN	Q92995.
InParanoid	Q92995.
OMA	IDESSVM.
OrthoDB	EOG9MD0JK.
PhylomeDB	Q92995.
Enzyme and pathway databases
BRENDA	3.1.2.15. 247.
Gene expression databases
ArrayExpress	Q92995.
Bgee	Q92995.
CleanEx	HS_USP13.
Genevestigator	Q92995.
GermOnline	ENSG00000058056. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. IPR016652. Ubiquitinyl_hydrolase. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00627. UBA. 2 hits. PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PIRSF	PIRSF016308. UBP. 1 hit.
SMART	SM00165. UBA. 2 hits. SM00290. ZnF_UBP. 1 hit. [Graphical view]
PROSITE	PS50030. UBA. 2 hits. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	33685.
SOURCE	Search...

Entry information

Entry name

UBP13_HUMAN

Accession

Primary (citable) accession number: Q92995
Secondary accession number(s): Q96B25

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 14, 2008
Last modified:	January 19, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.