Q92994 (TF3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor IIIB 90 kDa subunit
Short name=TFIIIB90
Short name=hTFIIIB90

Alternative name(s):
B-related factor 1
Short name=BRF-1
Short name=hBRF
TAF3B2
TATA box-binding protein-associated factor, RNA polymerase III, subunit 2

Gene names

Name:	BRF1
Synonyms:	BRF, GTF3B, TAF3B2, TAF3C

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Subunit structure	TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Ref.8
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.
Sequence caution	The sequence AAC50170.1 differs from that shown. Reason: Frameshift at positions 344 and 405.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro rRNA transcription Traceable author statement Ref.1. Source: ProtInc regulation of mRNA stability Inferred from electronic annotation. Source: Compara tRNA transcription Traceable author statement Ref.2. Source: ProtInc transcription initiation from RNA polymerase III promoter Traceable author statement Ref.1. Source: ProtInc
Cellular_component	transcription factor TFIIIB complex Non-traceable author statement Ref.2. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 9 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92994-1)

Also known as: hBRF1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92994-2)

Also known as: hBRF2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.
     293-338: SYTAGQRKLR...EIELENSRPK → IEEGGQTEAR...CGRGGFTLCP
     339-677: Missing.

Isoform 3 (identifier: Q92994-3)

Also known as: hBRF3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Isoform 4 (identifier: Q92994-4)

Also known as: hBRF4;

The sequence of this isoform differs from the canonical sequence as follows:
     159-242: NVYVLGKTFL...LLVAARMHDF → DSLRPASFPT...HLPMLGTVGC
     243-677: Missing.

Isoform 5 (identifier: Q92994-5)

The sequence of this isoform differs from the canonical sequence as follows:
     63-89: Missing.

Isoform 6 (identifier: Q92994-6)

The sequence of this isoform differs from the canonical sequence as follows:
     159-208: NVYVLGKTFL...KNHEVSMTAL → DSLRPASFPT...CPVRKFWSVG
     209-677: Missing.

Isoform 7 (identifier: Q92994-7)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Isoform 8 (identifier: Q92994-8)

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.
     459-459: R → RRDLSMPRCAKAKSQPHFPVLAQ

Note: No experimental confirmation available.

Isoform 9 (identifier: Q92994-9)

The sequence of this isoform differs from the canonical sequence as follows:
     1-238: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	Transcription factor IIIB 90 kDa subunit		PRO_0000119347
Regions
	Repeat	91 – 172	82	1
	Repeat	185 – 269	85	2
	Zinc finger	2 – 33	32	TFIIB-type
Sites
	Metal binding	6	1	Zinc By similarity
	Metal binding	9	1	Zinc By similarity
	Metal binding	25	1	Zinc By similarity
	Metal binding	28	1	Zinc By similarity
Amino acid modifications
	Modified residue	365	1	Phosphothreonine Ref.10 Ref.11
	Modified residue	433	1	Phosphoserine By similarity
	Modified residue	450	1	Phosphoserine Ref.10
	Modified residue	553	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	1 – 238	238	Missing in isoform 9.		VSP_045045
	Alternative sequence	1 – 204	204	Missing in isoform 2 and isoform 3.		VSP_006396
	Alternative sequence	1 – 115	115	Missing in isoform 7 and isoform 8.		VSP_044244
	Alternative sequence	63 – 89	27	Missing in isoform 5.		VSP_014697
	Alternative sequence	159 – 242	84	NVYVL…RMHDF → DSLRPASFPTWGCDLGVVTR VVTGVYPRCASRISVAGLCC LPSQEVLVCRMRGLHDMGVT VRDLWECGSPWQEGHLPMLG TVGC in isoform 4.		VSP_006399
	Alternative sequence	159 – 208	50	NVYVL…SMTAL → DSLRPASFPTWGCDLGVVTR VVTGVYPRCLHASQWPVCAA CPVRKFWSVG in isoform 6.		VSP_043835
	Alternative sequence	209 – 677	469	Missing in isoform 6.		VSP_043836
	Alternative sequence	243 – 677	435	Missing in isoform 4.		VSP_006400
	Alternative sequence	293 – 338	46	SYTAG…NSRPK → IEEGGQTEAREPPQASSWEG PSTTRRRSQLWHGCPGCGRG GFTLCP in isoform 2.		VSP_006397
	Alternative sequence	339 – 677	339	Missing in isoform 2.		VSP_006398
	Alternative sequence	459	1	R → RRDLSMPRCAKAKSQPHFPV LAQ in isoform 8.		VSP_045046
	Natural variant	542	1	V → M in a colorectal cancer sample; somatic mutation. Ref.12		VAR_035723
Experimental info
	Sequence conflict	88	1	N → D in AAC50170. Ref.1
	Sequence conflict	151	1	D → V in AAC50170. Ref.1
	Sequence conflict	224	1	R → G in AAC50170. Ref.1
	Sequence conflict	231	1	A → G in AAC50170. Ref.1
	Sequence conflict	339 – 340	2	AK → R in AAC50170. Ref.1
	Sequence conflict	437	1	D → N in BAH11919. Ref.4
	Sequence conflict	446	1	E → G in BAG53298. Ref.4
	Sequence conflict	594	1	P → T in AAC50170. Ref.1
	Sequence conflict	618	1	G → E in AAC50170. Ref.1
	Sequence conflict	640	1	E → G in BAG53298. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (hBRF1) [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 5866304B879EC1DE	FASTA	677	73,840
	10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 130 140 150 160 170 180 HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA 190 200 210 220 230 240 IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH 250 260 270 280 290 300 DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK 310 320 330 340 350 360 LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC 370 380 390 400 410 420 GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS 430 440 450 460 470 480 LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE 490 500 510 520 530 540 YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY 550 560 570 580 590 600 SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP 610 620 630 640 650 660 AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ 670 MMGSNDYGCD GDEDDGY
	Isoform 2 (hBRF2) [UniParc]. Checksum: 0F56B878F16C484B	FASTA	134	15,159
	10 20 30 40 50 60 MTALRLLQRM KRDWMHTGRR PSGLCGAALL VAARMHDFRR TVKEVISVVK VCESTLRKRL 70 80 90 100 110 120 TEFEDTPTSQ LTIDEFMKID LEEECDPPIE EGGQTEAREP PQASSWEGPS TTRRRSQLWH 130 GCPGCGRGGF TLCP
	Isoform 3 (hBRF3) [UniParc]. Checksum: EF95D8EDB737C5C4	FASTA	473	51,798
	10 20 30 40 50 60 MTALRLLQRM KRDWMHTGRR PSGLCGAALL VAARMHDFRR TVKEVISVVK VCESTLRKRL 70 80 90 100 110 120 TEFEDTPTSQ LTIDEFMKID LEEECDPPSY TAGQRKLRMK QLEQVLSKKL EEVEGEISSY 130 140 150 160 170 180 QDAIEIELEN SRPKAKGGLA SLAKDGSTED TASSLCGEED TEDEELEAAA SHLNKDLYRE 190 200 210 220 230 240 LLGGAPGSSE AAGSPEWGGR PPALGSLLDP LPTAASLGIS DSIRECISSQ SSDPKDASGD 250 260 270 280 290 300 GELDLSGIDD LEIDRYILNE SEARVKAELW MRENAEYLRE QREKEARIAK EKELGIYKEH 310 320 330 340 350 360 KPKKSCKRRE PIQASTAREA IEKMLEQKKI SSKINYSVLR GLSSAGGGSP HREDAQPEHS 370 380 390 400 410 420 ASARKLSRRR TPASRSGADP VTSVGKRLRP LVSTQPAKKV ATGEALLPSS PTLGAEPARP 430 440 450 460 470 QAVLVESGPV SYHADEEADE EEPDEEDGEP CVSALQMMGS NDYGCDGDED DGY
	Isoform 4 (hBRF4) [UniParc]. Checksum: B6FC691408BA8E26	FASTA	242	25,922
	10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 130 140 150 160 170 180 HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVDS LRPASFPTWG CDLGVVTRVV 190 200 210 220 230 240 TGVYPRCASR ISVAGLCCLP SQEVLVCRMR GLHDMGVTVR DLWECGSPWQ EGHLPMLGTV GC
	Isoform 5 [UniParc]. Checksum: EC6958C644660237	FASTA	650	71,119
	10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGRRHIHHLG NQLQLNQHCL DTAFNFFKMA VSRHLTRGRK MAHVIAACLY LVCRTEGTPH 130 140 150 160 170 180 MLLDLSDLLQ VNVYVLGKTF LLLARELCIN APAIDPCLYI PRFAHLLEFG EKNHEVSMTA 190 200 210 220 230 240 LRLLQRMKRD WMHTGRRPSG LCGAALLVAA RMHDFRRTVK EVISVVKVCE STLRKRLTEF 250 260 270 280 290 300 EDTPTSQLTI DEFMKIDLEE ECDPPSYTAG QRKLRMKQLE QVLSKKLEEV EGEISSYQDA 310 320 330 340 350 360 IEIELENSRP KAKGGLASLA KDGSTEDTAS SLCGEEDTED EELEAAASHL NKDLYRELLG 370 380 390 400 410 420 GAPGSSEAAG SPEWGGRPPA LGSLLDPLPT AASLGISDSI RECISSQSSD PKDASGDGEL 430 440 450 460 470 480 DLSGIDDLEI DRYILNESEA RVKAELWMRE NAEYLREQRE KEARIAKEKE LGIYKEHKPK 490 500 510 520 530 540 KSCKRREPIQ ASTAREAIEK MLEQKKISSK INYSVLRGLS SAGGGSPHRE DAQPEHSASA 550 560 570 580 590 600 RKLSRRRTPA SRSGADPVTS VGKRLRPLVS TQPAKKVATG EALLPSSPTL GAEPARPQAV 610 620 630 640 650 LVESGPVSYH ADEEADEEEP DEEDGEPCVS ALQMMGSNDY GCDGDEDDGY
	Isoform 6 [UniParc]. Checksum: 8761AE32A65FFE89	FASTA	208	22,309
	10 20 30 40 50 60 MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 70 80 90 100 110 120 DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 130 140 150 160 170 180 HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVDS LRPASFPTWG CDLGVVTRVV 190 200 TGVYPRCLHA SQWPVCAACP VRKFWSVG
	Isoform 7 [UniParc]. Checksum: 9C25CDA5DFE0F90E	FASTA	562	61,812
	10 20 30 40 50 60 MAVSRHLTRG RKMAHVIAAC LYLVCRTEGT PHMLLDLSDL LQVNVYVLGK TFLLLARELC 70 80 90 100 110 120 INAPAIDPCL YIPRFAHLLE FGEKNHEVSM TALRLLQRMK RDWMHTGRRP SGLCGAALLV 130 140 150 160 170 180 AARMHDFRRT VKEVISVVKV CESTLRKRLT EFEDTPTSQL TIDEFMKIDL EEECDPPSYT 190 200 210 220 230 240 AGQRKLRMKQ LEQVLSKKLE EVEGEISSYQ DAIEIELENS RPKAKGGLAS LAKDGSTEDT 250 260 270 280 290 300 ASSLCGEEDT EDEELEAAAS HLNKDLYREL LGGAPGSSEA AGSPEWGGRP PALGSLLDPL 310 320 330 340 350 360 PTAASLGISD SIRECISSQS SDPKDASGDG ELDLSGIDDL EIDRYILNES EARVKAELWM 370 380 390 400 410 420 RENAEYLREQ REKEARIAKE KELGIYKEHK PKKSCKRREP IQASTAREAI EKMLEQKKIS 430 440 450 460 470 480 SKINYSVLRG LSSAGGGSPH REDAQPEHSA SARKLSRRRT PASRSGADPV TSVGKRLRPL 490 500 510 520 530 540 VSTQPAKKVA TGEALLPSSP TLGAEPARPQ AVLVESGPVS YHADEEADEE EPDEEDGEPC 550 560 VSALQMMGSN DYGCDGDEDD GY
	Isoform 8 [UniParc]. Checksum: 9365EBA805C92B88	FASTA	584	64,275
	10 20 30 40 50 60 MAVSRHLTRG RKMAHVIAAC LYLVCRTEGT PHMLLDLSDL LQVNVYVLGK TFLLLARELC 70 80 90 100 110 120 INAPAIDPCL YIPRFAHLLE FGEKNHEVSM TALRLLQRMK RDWMHTGRRP SGLCGAALLV 130 140 150 160 170 180 AARMHDFRRT VKEVISVVKV CESTLRKRLT EFEDTPTSQL TIDEFMKIDL EEECDPPSYT 190 200 210 220 230 240 AGQRKLRMKQ LEQVLSKKLE EVEGEISSYQ DAIEIELENS RPKAKGGLAS LAKDGSTEDT 250 260 270 280 290 300 ASSLCGEEDT EDEELEAAAS HLNKDLYREL LGGAPGSSEA AGSPEWGGRP PALGSLLDPL 310 320 330 340 350 360 PTAASLGISD SIRECISSQS SDPKDASGDG ELDLSGIDDL EIDRRDLSMP RCAKAKSQPH 370 380 390 400 410 420 FPVLAQYILN ESEARVKAEL WMRENAEYLR EQREKEARIA KEKELGIYKE HKPKKSCKRR 430 440 450 460 470 480 EPIQASTARE AIEKMLEQKK ISSKINYSVL RGLSSAGGGS PHREDAQPEH SASARKLSRR 490 500 510 520 530 540 RTPASRSGAD PVTSVGKRLR PLVSTQPAKK VATGEALLPS SPTLGAEPAR PQAVLVESGP 550 560 570 580 VSYHADEEAD EEEPDEEDGE PCVSALQMMG SNDYGCDGDE DDGY
	Isoform 9 [UniParc]. Checksum: 340FA6DDD52479DC	FASTA	439	47,978
	10 20 30 40 50 60 MHDFRRTVKE VISVVKVCES TLRKRLTEFE DTPTSQLTID EFMKIDLEEE CDPPSYTAGQ 70 80 90 100 110 120 RKLRMKQLEQ VLSKKLEEVE GEISSYQDAI EIELENSRPK AKGGLASLAK DGSTEDTASS 130 140 150 160 170 180 LCGEEDTEDE ELEAAASHLN KDLYRELLGG APGSSEAAGS PEWGGRPPAL GSLLDPLPTA 190 200 210 220 230 240 ASLGISDSIR ECISSQSSDP KDASGDGELD LSGIDDLEID RYILNESEAR VKAELWMREN 250 260 270 280 290 300 AEYLREQREK EARIAKEKEL GIYKEHKPKK SCKRREPIQA STAREAIEKM LEQKKISSKI 310 320 330 340 350 360 NYSVLRGLSS AGGGSPHRED AQPEHSASAR KLSRRRTPAS RSGADPVTSV GKRLRPLVST 370 380 390 400 410 420 QPAKKVATGE ALLPSSPTLG AEPARPQAVL VESGPVSYHA DEEADEEEPD EEDGEPCVSA 430 LQMMGSNDYG CDGDEDDGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains." Wang Z., Roeder R.G. Proc. Natl. Acad. Sci. U.S.A. 92:7026-7030(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 264-281; 314-338; 441-469 AND 471-488.
[2]	"RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB." Mital R., Kobayashi R., Hernandez N. Mol. Cell. Biol. 16:7031-7042(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 248-254; 263-280; 313-320; 343-348; 380-396; 440-449; 471-488; 514-527; 538-561 AND 570-602.
[3]	"Alternatively spliced hBRF variants function at different RNA polymerase III promoters." McCulloch V., Hardin P., Peng W., Ruppert J.M., Lobo-Ruppert S.M. EMBO J. 19:4134-4143(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 7; 8 AND 9). Tissue: Brain and Hippocampus.
[5]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 5 AND 6). Tissue: Brain, Skin and Testis.
[8]	"TFIIIB is phosphorylated, disrupted and selectively released from tRNA promoters during mitosis in vivo." Fairley J.A., Scott P.H., White R.J. EMBO J. 22:5841-5850(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BDP1.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-553, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-365 AND SER-450, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-365, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] MET-542.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28838 mRNA. Translation: AAC50170.1. Frameshift. U75276 mRNA. Translation: AAB38876.1. AJ297406 mRNA. Translation: CAC04512.1. AJ297407 mRNA. Translation: CAC04513.1. AJ297408 mRNA. Translation: CAC04514.1. AK096471 mRNA. Translation: BAG53298.1. AK294899 mRNA. Translation: BAH11919.1. AK295579 mRNA. Translation: BAG58477.1. AL512355 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81908.1. CH471061 Genomic DNA. Translation: EAW81911.1. BC016743 mRNA. Translation: AAH16743.1. BC071637 mRNA. Translation: AAH71637.1. BC086856 mRNA. Translation: AAH86856.1. BC103859 mRNA. Translation: AAI03860.1.
IPI	IPI00016859. IPI00157643. IPI00216075. IPI00334140. IPI00607889. IPI01011010. IPI01012378.
PIR	I39065.
RefSeq	NP_001229715.1. NM_001242786.1. NP_001229716.1. NM_001242787.1. NP_001229717.1. NM_001242788.1. NP_001229718.1. NM_001242789.1. NP_001229719.1. NM_001242790.1. NP_001510.2. NM_001519.3. NP_663718.1. NM_145685.2.
UniGene	Hs.424484.
3D structure databases
ProteinModelPortal	Q92994.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92994. 2 interactions.
MINT	MINT-2859415.
STRING	9606.ENSP00000329029.
PTM databases
PhosphoSite	Q92994.
Polymorphism databases
DMDM	20455319.
Proteomic databases
PaxDb	Q92994.
PRIDE	Q92994.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000327359; ENSP00000329029; ENSG00000185024. ENST00000379937; ENSP00000369269; ENSG00000185024. ENST00000392557; ENSP00000376340; ENSG00000185024. ENST00000440513; ENSP00000388877; ENSG00000185024. ENST00000446501; ENSP00000389859; ENSG00000185024. ENST00000546474; ENSP00000448323; ENSG00000185024. ENST00000548421; ENSP00000446707; ENSG00000185024.
GeneID	2972.
KEGG	hsa:2972.
UCSC	uc001yql.2. human. uc001yqr.3. human. uc010axg.1. human. uc010typ.1. human.
Organism-specific databases
CTD	2972.
GeneCards	GC14M105675.
HGNC	HGNC:11551. BRF1.
MIM	604902. gene.
neXtProt	NX_Q92994.
PharmGKB	PA164741296.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1405.
HOVERGEN	HBG023540.
InParanoid	Q92994.
KO	K15196.
OMA	YGCDGDE.
OrthoDB	EOG4NGGMJ.
PhylomeDB	Q92994.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q92994.
Bgee	Q92994.
CleanEx	HS_BRF1.
Genevestigator	Q92994.
GermOnline	ENSG00000185024. Homo sapiens.
Family and domain databases
Gene3D	1.10.472.10. 2 hits.
InterPro	IPR011665. BRF1_TBP-bd. IPR013763. Cyclin-like. IPR000812. TFIIB. IPR013150. TFIIB_cyclin. IPR013137. Znf_TFIIB. [Graphical view]
PANTHER	PTHR11618. PTHR11618. 1 hit.
Pfam	PF07741. BRF1. 1 hit. PF08271. TF_Zn_Ribbon. 1 hit. PF00382. TFIIB. 2 hits. [Graphical view]
PRINTS	PR00685. TIFACTORIIB.
SMART	SM00385. CYCLIN. 2 hits. [Graphical view]
SUPFAM	SSF47954. Cyclin_like. 2 hits.
PROSITE	PS00782. TFIIB. False negative. PS51134. ZF_TFIIB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BRF1. human.
GenomeRNAi	2972.
NextBio	11787.
SOURCE	Search...

Entry information

Entry name

TF3B_HUMAN

Accession

Primary (citable) accession number: Q92994
Secondary accession number(s): B3KU36 , B4DIG5, B7Z2N3, F5H5Z7, F8WA46, Q13223, Q3SYD9, Q5PR24, Q6IQ02, Q96KX3, Q9HCW6, Q9HCW7, Q9HCW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.