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Q92989

- CLP1_HUMAN

UniProt

Q92989 - CLP1_HUMAN

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Protein

Polyribonucleotide 5'-hydroxyl-kinase Clp1

Gene
CLP1, HEAB
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (1 Publication, 1 Publication). Its role in tRNA splicing and maturation is required for cerebellar development (1 Publication, 1 Publication). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.4 Publications

Catalytic activityi

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.2 Publications
ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.2 Publications

Cofactori

Magnesium, manganese or nickel.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221ATP By similarity
Binding sitei62 – 621ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1296ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB-EC
  3. ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity Source: UniProtKB
  4. polydeoxyribonucleotide kinase activity Source: UniProtKB

GO - Biological processi

  1. cell death Source: UniProtKB-KW
  2. cerebellar cortex development Source: UniProtKB
  3. gene expression Source: Reactome
  4. mRNA 3'-end processing Source: UniProtKB
  5. mRNA splicing, via spliceosome Source: Reactome
  6. RNA splicing Source: Reactome
  7. siRNA loading onto RISC involved in RNA interference Source: UniProtKB
  8. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
  9. termination of RNA polymerase II transcription Source: Reactome
  10. transcription from RNA polymerase II promoter Source: Reactome
  11. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

mRNA processing, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Nickel, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_1096. Processing of Intronless Pre-mRNAs.
REACT_1849. mRNA 3'-end processing.
REACT_467. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide 5'-hydroxyl-kinase Clp1 (EC:2.7.1.78)
Alternative name(s):
Polyadenylation factor Clp1
Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1
Gene namesi
Name:CLP1
Synonyms:HEAB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:16999. CLP1.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. mRNA cleavage factor complex Source: UniProtKB-HAMAP
  2. nucleoplasm Source: Reactome
  3. nucleus Source: UniProtKB
  4. tRNA-intron endonuclease complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Neurodegeneration with progressive brain atrophy 1 (NBA1): A neurological syndrome affecting both the central and peripheral nervous systems characterized by onset of slow, progressive, neurodegenerative features and/or static encephalopathy after 6 months of age. Clinical features include brain malformations and microcephaly, developmental delays, severe intellectual disabilities, absent or delayed speech and spontaneous epileptic seizures. Brain displays mild atrophy of the cerebellum, pons and corpus callosum together with progressive microcephaly. A progressive spinal motor neuron loss is observed, due to increased neural precursor cell susceptibility to apoptosis. The disease is caused by mutations affecting the gene represented in this entry. Neurodegeneration is due to defects in tRNA splicing (1 Publication, 1 Publication).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi127 – 1282KS → AA: Abrogates RNA kinase activity. Abrogates complementation of tRNA splicing activity in yeast; when associated with A-128. 2 Publications
Mutagenesisi127 – 1271K → A: Abrogates RNA kinase activity and tRNA splicing activity. 2 Publications
Mutagenesisi151 – 1511D → A: Abrogates complementation of tRNA splicing activity in yeast. 2 Publications

Keywords - Diseasei

Disease mutation, Neurodegeneration

Organism-specific databases

PharmGKBiPA162382477.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Polyribonucleotide 5'-hydroxyl-kinase Clp1UniRule annotationPRO_0000089863Add
BLAST

Proteomic databases

MaxQBiQ92989.
PaxDbiQ92989.
PRIDEiQ92989.

PTM databases

PhosphoSiteiQ92989.

Expressioni

Gene expression databases

ArrayExpressiQ92989.
BgeeiQ92989.
CleanExiHS_CLP1.
GenevestigatoriQ92989.

Organism-specific databases

HPAiHPA049957.
HPA057770.

Interactioni

Subunit structurei

Component of the tRNA splicing endonuclease complex, composed of CLP1, TSEN2, TSEN15, TSEN34 and TSEN54 (1 Publication). Component of pre-mRNA cleavage complex II (CF-II). Also associates with numerous components of the pre-mRNA cleavage complex I (CF-I/CFIm), including NUDT21, CPSF2, CPSF3, CPSF6 and CPSF7. Interacts with CSTF2 and SYMPK.3 Publications

Protein-protein interaction databases

BioGridi116174. 15 interactions.
IntActiQ92989. 2 interactions.
MINTiMINT-3049820.
STRINGi9606.ENSP00000304704.

Structurei

3D structure databases

ProteinModelPortaliQ92989.
SMRiQ92989. Positions 14-423.

Family & Domainsi

Sequence similaritiesi

Belongs to the Clp1 family. Clp1 subfamily.

Phylogenomic databases

eggNOGiCOG5623.
HOGENOMiHOG000231935.
HOVERGENiHBG000921.
InParanoidiQ92989.
KOiK14399.
PhylomeDBiQ92989.
TreeFamiTF105795.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_03035. Clp1.
InterProiIPR028606. Clp1.
IPR029007. MobB-typ_P-loop.
IPR027417. P-loop_NTPase.
IPR010655. Pre-mRNA_cleavage_cplxII_Clp1.
[Graphical view]
PfamiPF06807. Clp1. 1 hit.
PF03205. MobB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q92989-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGEEANDDKK PTTKFELERE TELRFEVEAS QSVQLELLTG MAEIFGTELT    50
RNKKFTFDAG AKVAVFTWHG CSVQLSGRTE VAYVSKDTPM LLYLNTHTAL 100
EQMRRQAEKE EERGPRVMVV GPTDVGKSTV CRLLLNYAVR LGRRPTYVEL 150
DVGQGSVSIP GTMGALYIER PADVEEGFSI QAPLVYHFGS TTPGTNIKLY 200
NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGSGYQ ALVHAASAFE 250
VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDER 300
IREYFYGFRG CFYPHAFNVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL 350
KLVPVTPGRD MVHHLLSVST AEGTEENLSE TSVAGFIVVT SVDLEHQVFT 400
VLSPAPRPLP KNFLLIMDIR FMDLK 425
Length:425
Mass (Da):47,646
Last modified:February 1, 1997 - v1
Checksum:i640AF4768994CFE6
GO
Isoform 2 (identifier: Q92989-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-202: Missing.

Show »
Length:361
Mass (Da):40,696
Checksum:i661F193326BCB711
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti140 – 1401R → H in NBA1; decreased kinase activity, impaired formation of the tRNA splicing endonuclease complex and impaired ability to mediate tRNA splicing and maturation. 2 Publications
VAR_070952

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei139 – 20264Missing in isoform 2. VSP_041164Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73524 mRNA. Translation: AAC50780.1.
AK300232 mRNA. Translation: BAG62000.1.
AK313007 mRNA. Translation: BAG35843.1.
CH471076 Genomic DNA. Translation: EAW73770.1.
BC000446 mRNA. Translation: AAH00446.1.
CCDSiCCDS44600.1. [Q92989-2]
CCDS7964.1. [Q92989-1]
RefSeqiNP_001136069.1. NM_001142597.1. [Q92989-2]
NP_006822.1. NM_006831.2. [Q92989-1]
XP_006718488.1. XM_006718425.1. [Q92989-1]
UniGeneiHs.523687.

Genome annotation databases

EnsembliENST00000302731; ENSP00000304704; ENSG00000172409. [Q92989-2]
ENST00000525602; ENSP00000436066; ENSG00000172409. [Q92989-1]
ENST00000533682; ENSP00000434995; ENSG00000172409. [Q92989-1]
GeneIDi10978.
KEGGihsa:10978.
UCSCiuc001nkw.3. human. [Q92989-1]
uc010rjw.2. human. [Q92989-2]

Polymorphism databases

DMDMi13431366.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U73524 mRNA. Translation: AAC50780.1 .
AK300232 mRNA. Translation: BAG62000.1 .
AK313007 mRNA. Translation: BAG35843.1 .
CH471076 Genomic DNA. Translation: EAW73770.1 .
BC000446 mRNA. Translation: AAH00446.1 .
CCDSi CCDS44600.1. [Q92989-2 ]
CCDS7964.1. [Q92989-1 ]
RefSeqi NP_001136069.1. NM_001142597.1. [Q92989-2 ]
NP_006822.1. NM_006831.2. [Q92989-1 ]
XP_006718488.1. XM_006718425.1. [Q92989-1 ]
UniGenei Hs.523687.

3D structure databases

ProteinModelPortali Q92989.
SMRi Q92989. Positions 14-423.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116174. 15 interactions.
IntActi Q92989. 2 interactions.
MINTi MINT-3049820.
STRINGi 9606.ENSP00000304704.

PTM databases

PhosphoSitei Q92989.

Polymorphism databases

DMDMi 13431366.

Proteomic databases

MaxQBi Q92989.
PaxDbi Q92989.
PRIDEi Q92989.

Protocols and materials databases

DNASUi 10978.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000302731 ; ENSP00000304704 ; ENSG00000172409 . [Q92989-2 ]
ENST00000525602 ; ENSP00000436066 ; ENSG00000172409 . [Q92989-1 ]
ENST00000533682 ; ENSP00000434995 ; ENSG00000172409 . [Q92989-1 ]
GeneIDi 10978.
KEGGi hsa:10978.
UCSCi uc001nkw.3. human. [Q92989-1 ]
uc010rjw.2. human. [Q92989-2 ]

Organism-specific databases

CTDi 10978.
GeneCardsi GC11P057425.
HGNCi HGNC:16999. CLP1.
HPAi HPA049957.
HPA057770.
MIMi 608757. gene.
neXtProti NX_Q92989.
PharmGKBi PA162382477.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5623.
HOGENOMi HOG000231935.
HOVERGENi HBG000921.
InParanoidi Q92989.
KOi K14399.
PhylomeDBi Q92989.
TreeFami TF105795.

Enzyme and pathway databases

Reactomei REACT_1096. Processing of Intronless Pre-mRNAs.
REACT_1849. mRNA 3'-end processing.
REACT_467. mRNA Splicing - Major Pathway.

Miscellaneous databases

GenomeRNAii 10978.
NextBioi 41710.
PROi Q92989.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q92989.
Bgeei Q92989.
CleanExi HS_CLP1.
Genevestigatori Q92989.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_03035. Clp1.
InterProi IPR028606. Clp1.
IPR029007. MobB-typ_P-loop.
IPR027417. P-loop_NTPase.
IPR010655. Pre-mRNA_cleavage_cplxII_Clp1.
[Graphical view ]
Pfami PF06807. Clp1. 1 hit.
PF03205. MobB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AF10 is split by MLL and HEAB, a human homolog to a putative Caenorhabditis elegans ATP/GTP-binding protein in an invins(10;11)(p12;q23q12)."
    Tanabe S., Bohlander S.K., Vignon C.V., Espinosa R. III, Zhao N., Strissel P.L., Zeleznik-Le N.J., Rowley J.D.
    Blood 88:3535-3545(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Cerebellum and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors."
    de Vries H., Rueegsegger U., Huebner W., Friedlein A., Langen H., Keller W.
    EMBO J. 19:5895-5904(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION AS A COMPONENT OF THE PRE-MRNA CLEAVAGE COMPLEX II, ASSOCIATION WITH THE PRE-MRNA CLEAVAGE COMPLEX I, SUBCELLULAR LOCATION.
  6. "Identification of a human endonuclease complex reveals a link between tRNA splicing and pre-mRNA 3' end formation."
    Paushkin S.V., Patel M., Furia B.S., Peltz S.W., Trotta C.R.
    Cell 117:311-321(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH TSEN2; TSEN15; TSEN34 TSEN54, INTERACTION WITH CSTF2 AND SYMPK.
  7. "The human RNA kinase hClp1 is active on 3' transfer RNA exons and short interfering RNAs."
    Weitzer S., Martinez J.
    Nature 447:222-226(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, IDENTIFICATION IN A COMPLEX WITH TSEN2; TSEN15; TSEN34 TSEN54, MUTAGENESIS OF 127-LYS-SER-128.
  8. "Human RNA 5'-kinase (hClp1) can function as a tRNA splicing enzyme in vivo."
    Ramirez A., Shuman S., Schwer B.
    RNA 14:1737-1745(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF 127-LYS-SER-128 AND ASP-151.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: VARIANT NBA1 HIS-140, FUNCTION, IDENTIFICATION IN THE TRNA SPLICING ENDONUCLEASE COMPLEX, MUTAGENESIS OF LYS-127.
  11. Cited for: VARIANT NBA1 HIS-140, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCLP1_HUMAN
AccessioniPrimary (citable) accession number: Q92989
Secondary accession number(s): B2R7J6, B4DTI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 1997
Last modified: September 3, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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