Reviewed, UniProtKB/Swiss-Prot Q92985 (IRF7_HUMAN)

Names and origin

Protein names

Recommended name:
    Interferon regulatory factor 7
      Short name=IRF-7

Gene names

Name:

IRF7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
Subunit structure	Homodimer; phosphorylation-induced. Interacts with TICAM1 and TICAM2.
Subcellular location	Nucleus. Cytoplasm. Note= The phosphorylated and active form accumulates selectively in the nucleus.
Tissue specificity	Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Induction	By type I interferons.
Post-translational modification	In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
Sequence similarities	Belongs to the IRF family. Contains 1 tryptophan pentad repeat DNA-binding domain.

Ontologies

Keywords
Biological process	Antiviral defense Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Ref.2 Traceable author statement. Source: ProtInc response to virus Ref.3 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.3 Traceable author statement. Source: ProtInc nucleus Ref.3 Traceable author statement. Source: ProtInc
Molecular function	specific RNA polymerase II transcription factor activity Ref.2 Traceable author statement. Source: ProtInc transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q92985-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q92985-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     228-256: Missing.

Isoform C (identifier: Q92985-3)

Also known as: Gamma;

The sequence of this isoform differs from the canonical sequence as follows:
     152-164: GGPPGPFLAHTHA → AQGSLLGSCTGGQ
     165-503: Missing.

Notes: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform D (identifier: Q92985-4)

Also known as: H;

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MALAPE → MPVPERPAAGPDSPRPGTR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Interferon regulatory factor 7		PRO_0000154562
Regions
	DNA binding	13 – 122	110	Tryptophan pentad repeat
Amino acid modifications
	Modified residue	471	1	Phosphoserine By similarity
	Modified residue	472	1	Phosphoserine By similarity
	Modified residue	483	1	Phosphoserine By similarity
	Modified residue	484	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 6	6	MALAPE → MPVPERPAAGPDSPRPGTR in isoform D.		VSP_002757
	Alternative sequence	152 – 164	13	GGPPG…AHTHA → AQGSLLGSCTGGQ in isoform C.		VSP_002758
	Alternative sequence	165 – 503	339	Missing in isoform C.		VSP_002759
	Alternative sequence	228 – 256	29	Missing in isoform B.		VSP_002760
	Natural variant	179	1	K → E: dbSNP rs1061502.		VAR_027957
	Natural variant	412	1	Q → R: dbSNP rs1131665.		VAR_034017

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 9863C147514652DE	FASTA	503	54,278
	10 20 30 40 50 60 MALAPERAAP RVLFGEWLLG EISSGCYEGL QWLDEARTCF RVPWKHFARK DLSEADARIF 70 80 90 100 110 120 KAWAVARGRW PPSSRGGGPP PEAETAERAG WKTNFRCALR STRRFVMLRD NSGDPADPHK 130 140 150 160 170 180 VYALSRELCW REGPGTDQTE AEAPAAVPPP QGGPPGPFLA HTHAGLQAPG PLPAPAGDKG 190 200 210 220 230 240 DLLLQAVQQS CLADHLLTAS WGADPVPTKA PGEGQEGLPL TGACAGGPGL PAGELYGWAV 250 260 270 280 290 300 ETTPSPGPQP AALTTGEAAA PESPHQAEPY LSPSPSACTA VQEPSPGALD VTIMYKGRTV 310 320 330 340 350 360 LQKVVGHPSC TFLYGPPDPA VRATDPQQVA FPSPAELPDQ KQLRYTEELL RHVAPGLHLE 370 380 390 400 410 420 LRGPQLWARR MGKCKVYWEV GGPPGSASPS TPACLLPRNC DTPIFDFRVF FQELVEFRAR 430 440 450 460 470 480 QRRGSPRYTI YLGFGQDLSA GRPKEKSLVL VKLEPWLCRV HLEGTQREGV SSLDSSSLSL 490 500 CLSSANSLYD DIECFLMELE QPA
	Isoform B (Beta) [UniParc]. Checksum: F98A7B630497BDD0		474	51,459
	10 20 30 40 50 60 MALAPERAAP RVLFGEWLLG EISSGCYEGL QWLDEARTCF RVPWKHFARK DLSEADARIF 70 80 90 100 110 120 KAWAVARGRW PPSSRGGGPP PEAETAERAG WKTNFRCALR STRRFVMLRD NSGDPADPHK 130 140 150 160 170 180 VYALSRELCW REGPGTDQTE AEAPAAVPPP QGGPPGPFLA HTHAGLQAPG PLPAPAGDKG 190 200 210 220 230 240 DLLLQAVQQS CLADHLLTAS WGADPVPTKA PGEGQEGLPL TGACAGGEAA APESPHQAEP 250 260 270 280 290 300 YLSPSPSACT AVQEPSPGAL DVTIMYKGRT VLQKVVGHPS CTFLYGPPDP AVRATDPQQV 310 320 330 340 350 360 AFPSPAELPD QKQLRYTEEL LRHVAPGLHL ELRGPQLWAR RMGKCKVYWE VGGPPGSASP 370 380 390 400 410 420 STPACLLPRN CDTPIFDFRV FFQELVEFRA RQRRGSPRYT IYLGFGQDLS AGRPKEKSLV 430 440 450 460 470 LVKLEPWLCR VHLEGTQREG VSSLDSSSLS LCLSSANSLY DDIECFLMEL EQPA
	Isoform C (Gamma) [UniParc]. Checksum: 6FC0146AD190006D		164	18,036
	10 20 30 40 50 60 MALAPERAAP RVLFGEWLLG EISSGCYEGL QWLDEARTCF RVPWKHFARK DLSEADARIF 70 80 90 100 110 120 KAWAVARGRW PPSSRGGGPP PEAETAERAG WKTNFRCALR STRRFVMLRD NSGDPADPHK 130 140 150 160 VYALSRELCW REGPGTDQTE AEAPAAVPPP QAQGSLLGSC TGGQ
	Isoform D (H) [UniParc]. Checksum: 0A659B0BD1FE30F7		516	55,635
	10 20 30 40 50 60 MPVPERPAAG PDSPRPGTRR AAPRVLFGEW LLGEISSGCY EGLQWLDEAR TCFRVPWKHF 70 80 90 100 110 120 ARKDLSEADA RIFKAWAVAR GRWPPSSRGG GPPPEAETAE RAGWKTNFRC ALRSTRRFVM 130 140 150 160 170 180 LRDNSGDPAD PHKVYALSRE LCWREGPGTD QTEAEAPAAV PPPQGGPPGP FLAHTHAGLQ 190 200 210 220 230 240 APGPLPAPAG DKGDLLLQAV QQSCLADHLL TASWGADPVP TKAPGEGQEG LPLTGACAGG 250 260 270 280 290 300 PGLPAGELYG WAVETTPSPG PQPAALTTGE AAAPESPHQA EPYLSPSPSA CTAVQEPSPG 310 320 330 340 350 360 ALDVTIMYKG RTVLQKVVGH PSCTFLYGPP DPAVRATDPQ QVAFPSPAEL PDQKQLRYTE 370 380 390 400 410 420 ELLRHVAPGL HLELRGPQLW ARRMGKCKVY WEVGGPPGSA SPSTPACLLP RNCDTPIFDF 430 440 450 460 470 480 RVFFQELVEF RARQRRGSPR YTIYLGFGQD LSAGRPKEKS LVLVKLEPWL CRVHLEGTQR 490 500 510 EGVSSLDSSS LSLCLSSANS LYDDIECFLM ELEQPA

References

[1]	Grossman A., Nicholl J., Antonio L., Luethy R., Suggs S., Sutherland G.R., Mak T.W. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), VARIANT GLU-179. Tissue: Spleen.
[2]	"IRF-7, a new interferon regulatory factor associated with Epstein-Barr virus latency." Zhang L., Pagano J.S. Mol. Cell. Biol. 17:5748-5757(1997) [PubMed: 9315633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
[3]	"Characterization of the interferon regulatory factor-7 and its potential role in the transcription activation of interferon A genes." Au W.-C., Moore P.A., LaFleur D.W., Tombal B., Pitha P.M. J. Biol. Chem. 273:29210-29217(1998) [PubMed: 9786932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D), VARIANTS GLU-179 AND ARG-412.
[4]	"Phosphorylation-induced dimerization of interferon regulatory factor 7 unmasks DNA binding and a bipartite transactivation domain." Marie I.J., Smith E., Prakash A., Levy D.E. Mol. Cell. Biol. 20:8803-8814(2000) [PubMed: 11073981] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, SUBUNIT.
[5]	"IRF3 and IRF7 phosphorylation in virus-infected cells does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein." Smith E.J., Marie I.J., Prakash A., Garcia-Sastre A., Levy D.E. J. Biol. Chem. 276:8951-8957(2001) [PubMed: 11124948] [Abstract] Cited for: INHIBITION OF PHOSPHORYLATION BY VACCINIA VIRUS PROTEIN E3.
[6]	"LPS-TLR4 signaling to IRF-3/7 and NF-kappaB involves the toll adapters TRAM and TRIF." Fitzgerald K.A., Rowe D.C., Barnes B.J., Caffrey D.R., Visintin A., Latz E., Monks B., Pitha P.M., Golenbock D.T. J. Exp. Med. 198:1043-1055(2003) [PubMed: 14517278] [Abstract] Cited for: INTERACTION WITH TICAM2.
[7]	Erratum Fitzgerald K.A., Rowe D.C., Barnes B.J., Caffrey D.R., Visintin A., Latz E., Monks B., Pitha P.M., Golenbock D.T. J. Exp. Med. 198:1451-1451(2003)
[8]	"Mechanisms of the TRIF-induced interferon-stimulated response element and NF-kappaB activation and apoptosis pathways." Han K.J., Su X., Xu L.-G., Bin L.H., Zhang J., Shu H.-B. J. Biol. Chem. 279:15652-15661(2004) [PubMed: 14739303] [Abstract] Cited for: INTERACTION WITH TICAM1.
[9]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73036 mRNA. Translation: AAB17190.1. U53830 mRNA. Translation: AAB80686.1. U53831 mRNA. Translation: AAB80688.1. U53832 mRNA. Translation: AAB80690.1. U53832 mRNA. Translation: AAB80691.1. AF076494 mRNA. Translation: AAC70999.1.
RefSeq	NP_001563.2. NP_004020.1. NP_004022.2.
UniGene	Hs.166120
3D structure databases
HSSP	HSSP built from PDB template 2IRF based on UniProtKB P23906.
ModBase	Search...
PTM databases
PhosphoSite	Q92985.
Genome annotation databases
Ensembl	ENSG00000185507. Homo sapiens. [Contig view]
GeneID	3665.
KEGG	hsa:3665.
Organism-specific databases
HGNC	HGNC:6122. IRF7.
HPA	CAB017694.
MIM	605047. gene.
PharmGKB	PA29921.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q92985.
Gene expression databases
ArrayExpress	Q92985.
CleanEx	HS_IRF7.
GermOnline	ENSG00000185507. Homo sapiens.
Family and domain databases
InterPro	IPR001346. IRF. IPR017855. SMAD_dom-like. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.60.200.10. MH2_Dwarfin-type. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00605. IRF. 1 hit. [Graphical view]
PRINTS	PR00267. INTFRNREGFCT.
ProDom	PD002355. IRF. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00348. IRF. 1 hit. [Graphical view]
PROSITE	PS00601. IRF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14347.
SOURCE	Search...

Entry information

Entry name

IRF7_HUMAN

Accession

Primary (citable) accession number: Q92985
Secondary accession number(s): O00331 , O00332, O00333, O75924, Q9UE79

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 17, 2006
Last modified:	November 25, 2008
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)