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Protein

Transportin-1

Gene

TNPO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19 (By similarity). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571).By similarity5 Publications
(Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei468Important for interaction with cargo nuclear localization signals1 Publication1
Sitei738Important for interaction with cargo nuclear localization signals1 Publication1

GO - Molecular functioni

  • nuclear localization sequence binding Source: ProtInc
  • protein transporter activity Source: GO_Central
  • Ran GTPase binding Source: InterPro
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-450513 Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA
R-HSA-5620924 Intraflagellar transport

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Transportin-1
Alternative name(s):
Importin beta-2
Karyopherin beta-2
M9 region interaction protein
Short name:
MIP
Gene namesi
Name:TNPO1
Synonyms:KPNB2, MIP1, TRN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000083312.17
HGNCiHGNC:6401 TNPO1
MIMi602901 gene
neXtProtiNX_Q92973

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi468W → A: Abolishes interaction with the ADAR nuclear localization signal. Abolishes ADAR nuclear import. 1 Publication1
Mutagenesisi738W → A: Abolishes interaction with the ADAR nuclear localization signal. Abolishes ADAR nuclear import. 1 Publication1

Organism-specific databases

DisGeNETi3842
OpenTargetsiENSG00000083312
PharmGKBiPA30192

Polymorphism and mutation databases

BioMutaiTNPO1
DMDMi259016171

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207651 – 898Transportin-1Add BLAST898

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Isoform 2 (identifier: Q92973-2)
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ92973
MaxQBiQ92973
PaxDbiQ92973
PeptideAtlasiQ92973
PRIDEiQ92973
ProteomicsDBi75635
75636 [Q92973-2]
75637 [Q92973-3]

PTM databases

iPTMnetiQ92973
PhosphoSitePlusiQ92973
SwissPalmiQ92973

Expressioni

Gene expression databases

BgeeiENSG00000083312
CleanExiHS_TNPO1
ExpressionAtlasiQ92973 baseline and differential
GenevisibleiQ92973 HS

Organism-specific databases

HPAiCAB016325

Interactioni

Subunit structurei

Identified in a complex that contains TNPO1, RAN and RANBP1 (PubMed:9428644). Binds HNRPA1, HNRPA2, HNRNPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Interacts with isoform 1 and isoform 5 of ADAR/ADAR1 (via DRBM 3 domain) (PubMed:19124606, PubMed:24753571). Interacts with SNAI1 (via zinc fingers); the interaction mediates SNAI1 nuclear import (PubMed:19386897). Interacts with SNAI2 (via zinc fingers) (PubMed:19386897).By similarity6 Publications
(Microbial infection) Binds to HIV-1 Rev.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110040, 85 interactors
DIPiDIP-29335N
IntActiQ92973, 45 interactors
MINTiQ92973
STRINGi9606.ENSP00000336712

Structurei

Secondary structure

1898
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 30Combined sources14
Beta strandi31 – 33Combined sources3
Helixi35 – 48Combined sources14
Beta strandi49 – 51Combined sources3
Helixi52 – 63Combined sources12
Helixi70 – 83Combined sources14
Turni84 – 86Combined sources3
Helixi88 – 90Combined sources3
Helixi93 – 104Combined sources12
Turni105 – 108Combined sources4
Helixi112 – 128Combined sources17
Turni129 – 133Combined sources5
Beta strandi134 – 136Combined sources3
Helixi137 – 144Combined sources8
Helixi147 – 149Combined sources3
Helixi150 – 166Combined sources17
Helixi168 – 172Combined sources5
Beta strandi173 – 178Combined sources6
Helixi180 – 189Combined sources10
Helixi190 – 192Combined sources3
Helixi196 – 206Combined sources11
Helixi207 – 209Combined sources3
Turni210 – 213Combined sources4
Helixi215 – 218Combined sources4
Helixi221 – 231Combined sources11
Helixi237 – 253Combined sources17
Turni256 – 258Combined sources3
Helixi259 – 261Combined sources3
Helixi262 – 273Combined sources12
Helixi278 – 293Combined sources16
Beta strandi294 – 296Combined sources3
Helixi297 – 300Combined sources4
Helixi302 – 304Combined sources3
Helixi305 – 315Combined sources11
Helixi320 – 326Combined sources7
Turni327 – 329Combined sources3
Helixi334 – 339Combined sources6
Helixi340 – 342Combined sources3
Helixi345 – 361Combined sources17
Helixi367 – 370Combined sources4
Helixi383 – 398Combined sources16
Helixi399 – 402Combined sources4
Helixi403 – 405Combined sources3
Helixi406 – 414Combined sources9
Beta strandi416 – 418Combined sources3
Helixi419 – 435Combined sources17
Turni436 – 438Combined sources3
Helixi439 – 442Combined sources4
Beta strandi444 – 446Combined sources3
Helixi447 – 451Combined sources5
Turni452 – 456Combined sources5
Helixi460 – 467Combined sources8
Turni475 – 480Combined sources6
Turni482 – 485Combined sources4
Helixi488 – 497Combined sources10
Beta strandi498 – 500Combined sources3
Helixi502 – 518Combined sources17
Helixi520 – 523Combined sources4
Helixi524 – 526Combined sources3
Helixi527 – 532Combined sources6
Helixi536 – 541Combined sources6
Beta strandi543 – 545Combined sources3
Helixi546 – 556Combined sources11
Helixi561 – 564Combined sources4
Turni567 – 569Combined sources3
Helixi570 – 578Combined sources9
Helixi588 – 594Combined sources7
Helixi597 – 605Combined sources9
Helixi606 – 609Combined sources4
Helixi610 – 628Combined sources19
Helixi632 – 636Combined sources5
Turni638 – 640Combined sources3
Helixi647 – 658Combined sources12
Turni659 – 661Combined sources3
Helixi665 – 667Combined sources3
Helixi668 – 672Combined sources5
Helixi676 – 683Combined sources8
Helixi689 – 705Combined sources17
Helixi707 – 710Combined sources4
Helixi711 – 713Combined sources3
Helixi714 – 720Combined sources7
Helixi727 – 729Combined sources3
Helixi730 – 747Combined sources18
Helixi748 – 755Combined sources8
Helixi756 – 766Combined sources11
Beta strandi768 – 770Combined sources3
Helixi773 – 787Combined sources15
Helixi791 – 811Combined sources21
Helixi820 – 831Combined sources12
Helixi833 – 835Combined sources3
Helixi837 – 840Combined sources4
Helixi843 – 848Combined sources6
Helixi855 – 880Combined sources26
Helixi881 – 883Combined sources3
Helixi886 – 894Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QBKX-ray3.00B9-898[»]
2H4MX-ray3.05A/B376-898[»]
A/B9-343[»]
2OT8X-ray3.10A/B9-331[»]
A/B375-898[»]
2QMRX-ray3.00A/B/C/D9-898[»]
2Z5JX-ray3.40A9-898[»]
2Z5KX-ray2.60A9-898[»]
2Z5MX-ray3.00A9-898[»]
2Z5NX-ray3.20A9-898[»]
2Z5OX-ray3.20A9-898[»]
4FDDX-ray2.30A9-331[»]
A375-898[»]
4FQ3X-ray3.00A9-898[»]
4JLQX-ray3.05A9-331[»]
A375-898[»]
4OO6X-ray2.70A375-898[»]
A9-331[»]
5J3VX-ray3.05A/B9-331[»]
A/B375-898[»]
5TQCX-ray3.00A9-343[»]
A375-898[»]
ProteinModelPortaliQ92973
SMRiQ92973
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92973

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati19 – 46HEAT 11 PublicationAdd BLAST28
Domaini41 – 109Importin N-terminalPROSITE-ProRule annotationAdd BLAST69
Repeati51 – 89HEAT 21 PublicationAdd BLAST39
Repeati98 – 131HEAT 31 PublicationAdd BLAST34
Repeati137 – 174HEAT 41 PublicationAdd BLAST38
Repeati181 – 211HEAT 51 PublicationAdd BLAST31
Repeati224 – 251HEAT 61 PublicationAdd BLAST28
Repeati263 – 290HEAT 71 PublicationAdd BLAST28
Repeati306 – 397HEAT 81 PublicationAdd BLAST92
Repeati405 – 433HEAT 91 PublicationAdd BLAST29
Repeati445 – 472HEAT 101 PublicationAdd BLAST28
Repeati486 – 519HEAT 111 PublicationAdd BLAST34
Repeati527 – 560HEAT 121 PublicationAdd BLAST34
Repeati568 – 606HEAT 131 PublicationAdd BLAST39
Repeati614 – 665HEAT 141 PublicationAdd BLAST52
Repeati676 – 707HEAT 151 PublicationAdd BLAST32
Repeati715 – 748HEAT 161 PublicationAdd BLAST34
Repeati756 – 791HEAT 171 PublicationAdd BLAST36
Repeati799 – 832HEAT 181 PublicationAdd BLAST34
Repeati841 – 872HEAT 191 PublicationAdd BLAST32
Repeati875 – 895HEAT 201 PublicationAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi358 – 376Asp/Glu-rich (acidic)Add BLAST19

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2023 Eukaryota
ENOG410XPK2 LUCA
GeneTreeiENSGT00550000074720
HOGENOMiHOG000203940
HOVERGENiHBG058963
InParanoidiQ92973
KOiK18752
OMAiHNFPNGV
OrthoDBiEOG091G01O2
PhylomeDBiQ92973
TreeFamiTF300825

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50166 IMPORTIN_B_NT, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92973-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWDRQTKME YEWKPDEQGL QQILQLLKES QSPDTTIQRT VQQKLEQLNQ
60 70 80 90 100
YPDFNNYLIF VLTKLKSEDE PTRSLSGLIL KNNVKAHFQN FPNGVTDFIK
110 120 130 140 150
SECLNNIGDS SPLIRATVGI LITTIASKGE LQNWPDLLPK LCSLLDSEDY
160 170 180 190 200
NTCEGAFGAL QKICEDSAEI LDSDVLDRPL NIMIPKFLQF FKHSSPKIRS
210 220 230 240 250
HAVACVNQFI ISRTQALMLH IDSFIENLFA LAGDEEPEVR KNVCRALVML
260 270 280 290 300
LEVRMDRLLP HMHNIVEYML QRTQDQDENV ALEACEFWLT LAEQPICKDV
310 320 330 340 350
LVRHLPKLIP VLVNGMKYSD IDIILLKGDV EEDETIPDSE QDIRPRFHRS
360 370 380 390 400
RTVAQQHDED GIEEEDDDDD EIDDDDTISD WNLRKCSAAA LDVLANVYRD
410 420 430 440 450
ELLPHILPLL KELLFHHEWV VKESGILVLG AIAEGCMQGM IPYLPELIPH
460 470 480 490 500
LIQCLSDKKA LVRSITCWTL SRYAHWVVSQ PPDTYLKPLM TELLKRILDS
510 520 530 540 550
NKRVQEAACS AFATLEEEAC TELVPYLAYI LDTLVFAFSK YQHKNLLILY
560 570 580 590 600
DAIGTLADSV GHHLNKPEYI QMLMPPLIQK WNMLKDEDKD LFPLLECLSS
610 620 630 640 650
VATALQSGFL PYCEPVYQRC VNLVQKTLAQ AMLNNAQPDQ YEAPDKDFMI
660 670 680 690 700
VALDLLSGLA EGLGGNIEQL VARSNILTLM YQCMQDKMPE VRQSSFALLG
710 720 730 740 750
DLTKACFQHV KPCIADFMPI LGTNLNPEFI SVCNNATWAI GEISIQMGIE
760 770 780 790 800
MQPYIPMVLH QLVEIINRPN TPKTLLENTA ITIGRLGYVC PQEVAPMLQQ
810 820 830 840 850
FIRPWCTSLR NIRDNEEKDS AFRGICTMIS VNPSGVIQDF IFFCDAVASW
860 870 880 890
INPKDDLRDM FCKILHGFKN QVGDENWRRF SDQFPLPLKE RLAAFYGV
Length:898
Mass (Da):102,355
Last modified:September 22, 2009 - v2
Checksum:i7B880D9E7CA6798F
GO
Isoform 2 (identifier: Q92973-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MVWDRQTKM → M

Show »
Length:890
Mass (Da):101,310
Checksum:i4762DB4895472D10
GO
Isoform 3 (identifier: Q92973-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-118: Missing.

Show »
Length:848
Mass (Da):96,901
Checksum:i6DF034D06DC572B4
GO

Sequence cautioni

The sequence AAC50723 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH40340 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104L → S in AAB68948 (Ref. 3) Curated1
Sequence conflicti225I → T in AAC50723 (PubMed:8808633).Curated1
Sequence conflicti669Q → L in AAB68948 (Ref. 3) Curated1
Sequence conflicti880F → L in BAG62638 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0380281 – 9MVWDRQTKM → M in isoform 2. 2 Publications9
Alternative sequenceiVSP_03802969 – 118Missing in isoform 3. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70322 mRNA Translation: AAC50723.1 Different initiation.
U72069 mRNA Translation: AAB58254.1
U72395 mRNA Translation: AAB68948.1
AK301021 mRNA Translation: BAG62638.1
BC040340 mRNA Translation: AAH40340.1 Different initiation.
CCDSiCCDS4016.1 [Q92973-2]
CCDS43329.1 [Q92973-1]
RefSeqiNP_002261.3, NM_002270.3 [Q92973-1]
NP_694858.1, NM_153188.2 [Q92973-2]
XP_005248557.1, XM_005248500.2 [Q92973-2]
UniGeneiHs.482497

Genome annotation databases

EnsembliENST00000337273; ENSP00000336712; ENSG00000083312 [Q92973-1]
ENST00000506351; ENSP00000425118; ENSG00000083312 [Q92973-2]
ENST00000523768; ENSP00000428899; ENSG00000083312 [Q92973-3]
GeneIDi3842
KEGGihsa:3842
UCSCiuc003kci.5 human [Q92973-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTNPO1_HUMAN
AccessioniPrimary (citable) accession number: Q92973
Secondary accession number(s): B4DVC6, Q92957, Q92975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: June 20, 2018
This is version 194 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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