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Q92874

- DNSL2_HUMAN

UniProt

Q92874 - DNSL2_HUMAN

Protein

Deoxyribonuclease-1-like 2

Gene

DNASE1L2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Divalent cation-dependent acid DNA endonuclease involved in the breakdown of the nucleus during corneocyte formation of epidermal keratinocytes. May play an immune role by eliminating harmful DNA released into the extracellular environment by damaged epidermal cells.1 Publication

    Cofactori

    Divalent metal cations. Seems to require both calcium and magnesium.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei99 – 991By similarity
    Active sitei170 – 1701By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: ProtInc
    2. deoxyribonuclease activity Source: ProtInc
    3. DNA binding Source: ProtInc
    4. endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: InterPro

    GO - Biological processi

    1. DNA metabolic process Source: ProtInc

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyribonuclease-1-like 2 (EC:3.1.21.-)
    Alternative name(s):
    DNase I homolog protein DHP1
    Deoxyribonuclease I-like 2
    Short name:
    DNase I-like 2
    Gene namesi
    Name:DNASE1L2
    Synonyms:DHP1, DNAS1L2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:2958. DNASE1L2.

    Subcellular locationi

    Cytoplasm 1 Publication. Secreted Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27429.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 299279Deoxyribonuclease-1-like 2PRO_0000007286Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi209 ↔ 245Essential for enzymatic activityBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ92874.
    PRIDEiQ92874.

    Expressioni

    Tissue specificityi

    Preferentially expressed in the skin and up-regulated during keratinocytes differentiation. Highly abundant (at protein level) in the stratum granulosum.1 Publication

    Inductioni

    Up-regulated by inflammatory cytokines.1 Publication

    Gene expression databases

    ArrayExpressiQ92874.
    BgeeiQ92874.
    CleanExiHS_DNASE1L2.
    GenevestigatoriQ92874.

    Organism-specific databases

    HPAiHPA044714.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ABL1P005191EBI-1751995,EBI-375543
    FYNP062411EBI-1751995,EBI-515315

    Protein-protein interaction databases

    IntActiQ92874. 2 interactions.
    MINTiMINT-4722563.
    STRINGi9606.ENSP00000316938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92874.
    SMRiQ92874. Positions 22-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNase I family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG46375.
    HOGENOMiHOG000059570.
    HOVERGENiHBG051368.
    InParanoidiQ92874.
    KOiK11995.
    OMAiYQYPDPE.
    OrthoDBiEOG73V6KW.
    PhylomeDBiQ92874.
    TreeFamiTF329541.

    Family and domain databases

    Gene3Di3.60.10.10. 1 hit.
    InterProiIPR018057. Deoxyribonuclease-1_AS.
    IPR016202. DNase_I.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view]
    PANTHERiPTHR11371. PTHR11371. 1 hit.
    PfamiPF03372. Exo_endo_phos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000988. DNase_I_euk. 1 hit.
    PRINTSiPR00130. DNASEI.
    SMARTiSM00476. DNaseIc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.
    PROSITEiPS00919. DNASE_I_1. 1 hit.
    PS00918. DNASE_I_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q92874-1) [UniParc]FASTAAdd to Basket

    Also known as: DNAS1L2-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGGPRALLAA LWALEAAGTA ALRIGAFNIQ SFGDSKVSDP ACGSIIAKIL    50
    AGYDLALVQE VRDPDLSAVS ALMEQINSVS EHEYSFVSSQ PLGRDQYKEM 100
    YLFVYRKDAV SVVDTYLYPD PEDVFSREPF VVKFSAPGTG ERAPPLPSRR 150
    ALTPPPLPAA AQNLVLIPLH AAPHQAVAEI DALYDVYLDV IDKWGTDDML 200
    FLGDFNADCS YVRAQDWAAI RLRSSEVFKW LIPDSADTTV GNSDCAYDRI 250
    VACGARLRRS LKPQSATVHD FQEEFGLDQT QALAISDHFP VEVTLKFHR 299
    Length:299
    Mass (Da):32,853
    Last modified:February 1, 1997 - v1
    Checksum:i1B66A5590D33D0A6
    GO
    Isoform 2 (identifier: Q92874-2) [UniParc]FASTAAdd to Basket

    Also known as: DNAS1L2-S

    The sequence of this isoform differs from the canonical sequence as follows:
         140-160: Missing.

    Note: Specifically expressed in peripheral blood leukocytes.

    Show »
    Length:278
    Mass (Da):30,707
    Checksum:i076B44856A08D0BE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei140 – 16021Missing in isoform 2. 1 PublicationVSP_053879Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62647 mRNA. Translation: AAB63981.1.
    AY298957 mRNA. Translation: AAQ73759.1.
    AY298958 Genomic DNA. Translation: AAQ73760.1.
    AY298958 Genomic DNA. Translation: AAQ73761.1.
    AK098028 mRNA. Translation: BAG53566.1.
    AC009065 Genomic DNA. No translation available.
    CH471112 Genomic DNA. Translation: EAW85526.1.
    CH471112 Genomic DNA. Translation: EAW85527.1.
    CH471112 Genomic DNA. Translation: EAW85528.1.
    CH471112 Genomic DNA. Translation: EAW85529.1.
    BC035205 mRNA. Translation: AAH35205.1.
    BC063710 mRNA. Translation: AAH63710.1.
    CCDSiCCDS42105.1. [Q92874-1]
    RefSeqiNP_001365.1. NM_001374.2. [Q92874-1]
    XP_005255209.1. XM_005255152.2. [Q92874-1]
    XP_006720919.1. XM_006720856.1. [Q92874-1]
    UniGeneiHs.103503.

    Genome annotation databases

    EnsembliENST00000320700; ENSP00000316938; ENSG00000167968. [Q92874-1]
    ENST00000382437; ENSP00000371874; ENSG00000167968. [Q92874-2]
    ENST00000564065; ENSP00000454562; ENSG00000167968. [Q92874-1]
    ENST00000567494; ENSP00000455358; ENSG00000167968. [Q92874-1]
    GeneIDi1775.
    KEGGihsa:1775.
    UCSCiuc002cpn.3. human.
    uc002cpo.3. human. [Q92874-1]

    Polymorphism databases

    DMDMi2494172.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62647 mRNA. Translation: AAB63981.1 .
    AY298957 mRNA. Translation: AAQ73759.1 .
    AY298958 Genomic DNA. Translation: AAQ73760.1 .
    AY298958 Genomic DNA. Translation: AAQ73761.1 .
    AK098028 mRNA. Translation: BAG53566.1 .
    AC009065 Genomic DNA. No translation available.
    CH471112 Genomic DNA. Translation: EAW85526.1 .
    CH471112 Genomic DNA. Translation: EAW85527.1 .
    CH471112 Genomic DNA. Translation: EAW85528.1 .
    CH471112 Genomic DNA. Translation: EAW85529.1 .
    BC035205 mRNA. Translation: AAH35205.1 .
    BC063710 mRNA. Translation: AAH63710.1 .
    CCDSi CCDS42105.1. [Q92874-1 ]
    RefSeqi NP_001365.1. NM_001374.2. [Q92874-1 ]
    XP_005255209.1. XM_005255152.2. [Q92874-1 ]
    XP_006720919.1. XM_006720856.1. [Q92874-1 ]
    UniGenei Hs.103503.

    3D structure databases

    ProteinModelPortali Q92874.
    SMRi Q92874. Positions 22-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q92874. 2 interactions.
    MINTi MINT-4722563.
    STRINGi 9606.ENSP00000316938.

    Polymorphism databases

    DMDMi 2494172.

    Proteomic databases

    PaxDbi Q92874.
    PRIDEi Q92874.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000320700 ; ENSP00000316938 ; ENSG00000167968 . [Q92874-1 ]
    ENST00000382437 ; ENSP00000371874 ; ENSG00000167968 . [Q92874-2 ]
    ENST00000564065 ; ENSP00000454562 ; ENSG00000167968 . [Q92874-1 ]
    ENST00000567494 ; ENSP00000455358 ; ENSG00000167968 . [Q92874-1 ]
    GeneIDi 1775.
    KEGGi hsa:1775.
    UCSCi uc002cpn.3. human.
    uc002cpo.3. human. [Q92874-1 ]

    Organism-specific databases

    CTDi 1775.
    GeneCardsi GC16P002285.
    H-InvDB HIX0038772.
    HGNCi HGNC:2958. DNASE1L2.
    HPAi HPA044714.
    MIMi 602622. gene.
    neXtProti NX_Q92874.
    PharmGKBi PA27429.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG46375.
    HOGENOMi HOG000059570.
    HOVERGENi HBG051368.
    InParanoidi Q92874.
    KOi K11995.
    OMAi YQYPDPE.
    OrthoDBi EOG73V6KW.
    PhylomeDBi Q92874.
    TreeFami TF329541.

    Miscellaneous databases

    GeneWikii DNASE1L2.
    GenomeRNAii 1775.
    NextBioi 7227.
    PROi Q92874.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q92874.
    Bgeei Q92874.
    CleanExi HS_DNASE1L2.
    Genevestigatori Q92874.

    Family and domain databases

    Gene3Di 3.60.10.10. 1 hit.
    InterProi IPR018057. Deoxyribonuclease-1_AS.
    IPR016202. DNase_I.
    IPR005135. Endo/exonuclease/phosphatase.
    [Graphical view ]
    PANTHERi PTHR11371. PTHR11371. 1 hit.
    Pfami PF03372. Exo_endo_phos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000988. DNase_I_euk. 1 hit.
    PRINTSi PR00130. DNASEI.
    SMARTi SM00476. DNaseIc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    PROSITEi PS00919. DNASE_I_1. 1 hit.
    PS00918. DNASE_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I."
      Rodriguez A.M., Rodin D., Nomura H., Morton C.C., Weremowicz S., Schneider M.C.
      Genomics 42:507-513(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Characterization of the human DNAS1L2 gene and the molecular mechanism for its transcriptional activation induced by inflammatory cytokines."
      Shiokawa D., Matsushita T., Kobayashi T., Matsumoto Y., Tanuma S.I.
      Genomics 84:95-105(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), INDUCTION, COFACTOR, ALTERNATIVE SPLICING.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    4. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Skin.
    7. Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDNSL2_HUMAN
    AccessioniPrimary (citable) accession number: Q92874
    Secondary accession number(s): E9PBY4, Q6JVM2, Q6JVM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3