Reviewed,
UniProtKB/Swiss-Prot Q92843 (B2CL2_HUMAN)
Last modified
January 19, 2010.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Bcl-2-like protein 2 Short name=Bcl2-L-2 Alternative name(s): Apoptosis regulator Bcl-W | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 193 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX. Ref.1 |
| Subcellular location | Mitochondrion membrane; Peripheral membrane protein. Note: Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane. Ref.7 Ref.8 |
| Tissue specificity | Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines. Ref.7 |
| Domain | The BH4 motif seems to be involved in the anti-apoptotic function. The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2. |
| Sequence similarities | Belongs to the Bcl-2 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Apoptosis |
| Cellular component | Membrane Mitochondrion |
| Coding sequence diversity | Polymorphism |
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | anti-apoptosis Traceable author statement. Source: ProtInc apoptosisInferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Ref.1Traceable author statement. Source: ProtInc |
| Cellular component | extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BAD | Q92934 | 1 | EBI-707714,EBI-700771 | |
| Bad | Q61337 | 1 | EBI-707714,EBI-400328 | From a different organism. |
| BBC3 | Q9BXH1 | 2 | EBI-707714,EBI-519884 | |
| BCL2L11 | O43521 | 2 | EBI-707714,EBI-526406 | |
| Bcl2l11 | O54918 | 1 | EBI-707714,EBI-526067 | From a different organism. |
| BID | P55957 | 2 | EBI-707714,EBI-519672 | |
| Bid | P70444 | 1 | EBI-707714,EBI-783400 | From a different organism. |
| BIK | Q13323 | 2 | EBI-707714,EBI-700794 | |
| Bmf | Q91ZE9 | 2 | EBI-707714,EBI-708032 | From a different organism. |
| HRK | O00198 | 1 | EBI-707714,EBI-701322 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 193 | 193 | Bcl-2-like protein 2 | PRO_0000143066 | |||||||||||||||||||||||||
Regions | |||||||||||||||||||||||||||||
| Motif | 9 – 29 | 21 | BH4 | ||||||||||||||||||||||||||
| Motif | 85 – 104 | 20 | BH1 | ||||||||||||||||||||||||||
| Motif | 136 – 151 | 16 | BH2 | ||||||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||||||
| Modified residue | 192 | 1 | Phosphoserine Ref.9 | ||||||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||||||
| Natural variant | 133 | 1 | R → Q: dbSNP rs910332. | VAR_048418 | |||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||
| Helix | 10 – 23 | 14 | |||||||||||||||||||||||||||
| Helix | 42 – 56 | 15 | |||||||||||||||||||||||||||
| Helix | 60 – 70 | 11 | |||||||||||||||||||||||||||
| Helix | 76 – 87 | 12 | |||||||||||||||||||||||||||
| Helix | 94 – 112 | 19 | |||||||||||||||||||||||||||
| Helix | 116 – 132 | 17 | |||||||||||||||||||||||||||
| Helix | 135 – 141 | 7 | |||||||||||||||||||||||||||
| Helix | 144 – 148 | 5 | |||||||||||||||||||||||||||
| Beta strand | 151 – 154 | 4 | |||||||||||||||||||||||||||
| Helix | 156 – 168 | 13 | |||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Bcl-w, a novel member of the Bcl-2 family, promotes cell survival." Gibson L., Holmgreen S.P., Huang D.C., Bernard O., Copeland N.G., Jenkins N.A., Sutherland G.R., Baker E., Adams J.M., Cory S. Oncogene 13:665-675(1996) [PubMed: 8761287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. |
| [2] | "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed: 9039502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lung. |
| [7] | "Tissue expression and subcellular localization of the pro-survival molecule Bcl-w." O'Reilly L.A., Print C., Hausmann G., Moriishi K., Cory S., Huang D.C.S., Strasser A. Cell Death Differ. 8:486-494(2001) [PubMed: 11423909] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [8] | "Proapoptotic BH3-only proteins trigger membrane integration of prosurvival Bcl-w and neutralize its activity." Wilson-Annan J., O'Reilly L.A., Crawford S.A., Hausmann G., Beaumont J.G., Parma L.P., Chen L., Lackmann M., Lithgow T., Hinds M.G., Day C.L., Adams J.M., Huang D.C.S. J. Cell Biol. 162:877-887(2003) [PubMed: 12952938] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [9] | "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192, MASS SPECTROMETRY. |
| [10] | "The structure of Bcl-w reveals a role for the C-terminal residues in modulating biological activity." Hinds M.G., Lackmann M., Skea G.L., Harrison P.J., Huang D.C.S., Day C.L. EMBO J. 22:1497-1507(2003) [PubMed: 12660157] [Abstract] Cited for: STRUCTURE BY NMR OF 1-183. |
| [11] | "Solution structure of human BCL-w: modulation of ligand binding by the C-terminal helix." Denisov A.Y., Madiraju M.S.R., Chen G., Khadir A., Beauparlant P., Attardo G., Shore G.C., Gehring K. J. Biol. Chem. 278:21124-21128(2003) [PubMed: 12651847] [Abstract] Cited for: STRUCTURE BY NMR OF 2-171. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | U59747 mRNA. Translation: AAB09055.1. D87461 mRNA. Translation: BAA19666.2. Different initiation. BT019549 mRNA. Translation: AAV38356.1. AK289519 mRNA. Translation: BAF82208.1. CH471078 Genomic DNA. Translation: EAW66169.1. BC021198 mRNA. Translation: AAH21198.1. BC104789 mRNA. Translation: AAI04790.1. BC113522 mRNA. Translation: AAI13523.1. | ||||||||||||||||||||||||
| IPI | IPI00023787. | ||||||||||||||||||||||||
| RefSeq | NP_004041.1. | ||||||||||||||||||||||||
| UniGene | Hs.410026 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| DIP | DIP-33700N. | ||||||||||||||||||||||||
| IntAct | Q92843. 11 interactions. | ||||||||||||||||||||||||
| STRING | Q92843. | ||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||
| PhosphoSite | Q92843. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PRIDE | Q92843. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENST00000250405; ENSP00000250405; ENSG00000129473; Homo sapiens. [Genome view] | ||||||||||||||||||||||||
| GeneID | 599. | ||||||||||||||||||||||||
| KEGG | hsa:599. | ||||||||||||||||||||||||
| UCSC | uc001wjg.2. human. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| CTD | 599. | ||||||||||||||||||||||||
| GeneCards | GC14P022845. | ||||||||||||||||||||||||
| HGNC | HGNC:995. BCL2L2. | ||||||||||||||||||||||||
| HPA | CAB002048. | ||||||||||||||||||||||||
| MIM | 601931. gene. | ||||||||||||||||||||||||
| PharmGKB | PA25307. | ||||||||||||||||||||||||
| HUGE | Search... | ||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| eggNOG | prNOG12634. | ||||||||||||||||||||||||
| HOGENOM | HBG717457. | ||||||||||||||||||||||||
| HOVERGEN | Q92843. | ||||||||||||||||||||||||
| InParanoid | Q92843. | ||||||||||||||||||||||||
| OrthoDB | EOG99GP2S. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q92843. | ||||||||||||||||||||||||
| Bgee | Q92843. | ||||||||||||||||||||||||
| CleanEx | HS_BCL2L2. | ||||||||||||||||||||||||
| Genevestigator | Q92843. | ||||||||||||||||||||||||
| GermOnline | ENSG00000129473. Homo sapiens. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR013280. Apop_reg_BclW. IPR002475. BCL2_apoptsis. IPR000712. Bcl2_BH. IPR020717. Bcl2_BH1_motif_CS. IPR020726. Bcl2_BH2_motif_CS. IPR003093. Bcl2_BH4. IPR020731. Bcl2_BH4_motif_CS. [Graphical view] | ||||||||||||||||||||||||
| Pfam | PF00452. Bcl-2. 1 hit. PF02180. BH4. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PRINTS | PR01865. APOPREGBCLW. PR01862. BCL2FAMILY. | ||||||||||||||||||||||||
| SMART | SM00337. BCL. 1 hit. SM00265. BH4. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| PROSITE | PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01260. BH4_1. 1 hit. PS50063. BH4_2. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| NextBio | 2439. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | B2CL2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q92843 Secondary accession number(s): A8K0F4, Q2M3U0, Q5U0H4 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


