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Q92839 (HYAS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronan synthase 1

EC=2.4.1.212
Alternative name(s):
Hyaluronate synthase 1
Hyaluronic acid synthase 1
Short name=HA synthase 1
Short name=HuHAS1
Gene names
Name:HAS1
Synonyms:HAS
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length578 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate By similarity.

Catalytic activity

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).

UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactor

Magnesium.

Pathway

Glycan biosynthesis; hyaluronan biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Tissue specificity

Widely expressed. Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine. Ref.1

Sequence similarities

Belongs to the NodC/HAS family.

Sequence caution

The sequence BAA12351.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Traceable author statement. Source: Reactome

cell adhesion

Traceable author statement Ref.2. Source: ProtInc

cellular response to platelet-derived growth factor stimulus

Inferred from direct assay PubMed 17324121. Source: UniProtKB

extracellular matrix assembly

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular polysaccharide biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

glycosaminoglycan biosynthetic process

Traceable author statement Ref.2. Source: ProtInc

glycosaminoglycan metabolic process

Traceable author statement. Source: Reactome

hyaluronan biosynthetic process

Traceable author statement. Source: Reactome

hyaluronan metabolic process

Traceable author statement. Source: Reactome

negative regulation of fibroblast migration

Inferred from mutant phenotype PubMed 19577615. Source: UniProtKB

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentintegral component of plasma membrane

Traceable author statement PubMed 9169154. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionhyaluronan synthase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 578578Hyaluronan synthase 1
PRO_0000197169

Regions

Topological domain1 – 2525Cytoplasmic Potential
Transmembrane26 – 4621Helical; Name=1; Potential
Topological domain47 – 526Extracellular Potential
Transmembrane53 – 7321Helical; Name=2; Potential
Topological domain74 – 399326Cytoplasmic Potential
Transmembrane400 – 42021Helical; Name=3; Potential
Topological domain421 – 43010Extracellular Potential
Transmembrane431 – 45121Helical; Name=4; Potential
Topological domain452 – 4576Cytoplasmic Potential
Transmembrane458 – 47821Helical; Name=5; Potential
Topological domain479 – 49719Extracellular Potential
Transmembrane498 – 51821Helical; Name=6; Potential
Topological domain519 – 54022Cytoplasmic Potential
Transmembrane541 – 56121Helical; Name=7; Potential
Topological domain562 – 57817Extracellular Potential

Natural variations

Natural variant141C → R. Ref.1 Ref.2
Corresponds to variant rs7248778 [ dbSNP | Ensembl ].
VAR_047025

Experimental info

Sequence conflict1 – 22MR → RS in BAA12351. Ref.1
Sequence conflict341G → A in BAA12351. Ref.1
Sequence conflict1281L → V in BAA12351. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q92839 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: 2FE3A44B0D5380FF

FASTA57864,832
        10         20         30         40         50         60 
MRQQDAPKPT PAACRCSGLA RRVLTIAFAL LILGLMTWAY AAGVPLASDR YGLLAFGLYG 

        70         80         90        100        110        120 
AFLSAHLVAQ SLFAYLEHRR VAAAARGPLD AATARSVALT ISAYQEDPAY LRQCLASARA 

       130        140        150        160        170        180 
LLYPRARLRV LMVVDGNRAE DLYMVDMFRE VFADEDPATY VWDGNYHQPW EPAAAGAVGA 

       190        200        210        220        230        240 
GAYREVEAED PGRLAVEALV RTRRCVCVAQ RWGGKREVMY TAFKALGDSV DYVQVCDSDT 

       250        260        270        280        290        300 
RLDPMALLEL VRVLDEDPRV GAVGGDVRIL NPLDSWVSFL SSLRYWVAFN VERACQSYFH 

       310        320        330        340        350        360 
CVSCISGPLG LYRNNLLQQF LEAWYNQKFL GTHCTFGDDR HLTNRMLSMG YATKYTSRSR 

       370        380        390        400        410        420 
CYSETPSSFL RWLSQQTRWS KSYFREWLYN ALWWHRHHAW MTYEAVVSGL FPFFVAATVL 

       430        440        450        460        470        480 
RLFYAGRPWA LLWVLLCVQG VALAKAAFAA WLRGCLRMVL LSLYAPLYMC GLLPAKFLAL 

       490        500        510        520        530        540 
VTMNQSGWGT SGRRKLAANY VPLLPLALWA LLLLGGLVRS VAHEARADWS GPSRAAEAYH 

       550        560        570 
LAAGAGAYVG YWVAMLTLYW VGVRRLCRRR TGGYRVQV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of human hyaluronan synthase."
Itano N., Kimata K.
Biochem. Biophys. Res. Commun. 222:816-820(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-14, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Functional cloning of the cDNA for a human hyaluronan synthase."
Shyjan A.M., Heldin P., Butcher E.C., Yoshino T., Briskin M.J.
J. Biol. Chem. 271:23395-23399(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-14.
Tissue: Lymph node.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84424 mRNA. Translation: BAA12351.1. Different initiation.
U59269 mRNA. Translation: AAC50706.1.
AC018755 Genomic DNA. Translation: AAF87845.1.
CH471135 Genomic DNA. Translation: EAW72038.1.
CCDSCCDS12838.1.
PIRJC4812.
RefSeqNP_001514.2. NM_001523.2.
UniGeneHs.57697.

3D structure databases

ProteinModelPortalQ92839.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109286. 4 interactions.
STRING9606.ENSP00000222115.

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.
TCDB4.D.1.2.1. the putative vectorial glycosyl polymerization (vgp) family.

PTM databases

PhosphoSiteQ92839.

Polymorphism databases

DMDM209572627.

Proteomic databases

PaxDbQ92839.
PRIDEQ92839.

Protocols and materials databases

DNASU3036.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000222115; ENSP00000222115; ENSG00000105509.
GeneID3036.
KEGGhsa:3036.
UCSCuc002pxn.1. human.

Organism-specific databases

CTD3036.
GeneCardsGC19M052216.
HGNCHGNC:4818. HAS1.
MIM601463. gene.
neXtProtNX_Q92839.
PharmGKBPA29194.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1215.
HOGENOMHOG000112847.
HOVERGENHBG000189.
InParanoidQ92839.
KOK00752.
PhylomeDBQ92839.
TreeFamTF332506.

Enzyme and pathway databases

BRENDA2.4.1.212. 2681.
ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
UniPathwayUPA00341.

Gene expression databases

ArrayExpressQ92839.
BgeeQ92839.
CleanExHS_HAS1.
GenevestigatorQ92839.

Family and domain databases

Gene3D3.90.550.10. 2 hits.
InterProIPR026107. HAS/NodC.
IPR028385. HAS1.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF4. PTHR22913:SF4. 1 hit.
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Other

GeneWikiHAS1.
GenomeRNAi3036.
NextBio12018.
PROQ92839.
SOURCESearch...

Entry information

Entry nameHYAS1_HUMAN
AccessionPrimary (citable) accession number: Q92839
Secondary accession number(s): Q14470, Q9NS49
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: October 14, 2008
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM