Q92839 (HAS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan synthase 1
EC=2.4.1.212

Alternative name(s):
Hyaluronate synthase 1
Hyaluronic acid synthase 1
Short name=HA synthase 1
Short name=HuHAS1

Gene names

Name:	HAS1
Synonyms:	HAS

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	578 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Highly expressed in ovary followed by spleen, thymus, prostate, testes and large intestine. Weakly expressed in small intestine.
Sequence similarities	Belongs to the NodC/HAS family.
Sequence caution	The sequence BAA12351.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome cell adhesion Traceable author statement Ref.2. Source: ProtInc cellular response to platelet-derived growth factor stimulus Inferred from direct assay PubMed 17324121. Source: UniProtKB hyaluronan biosynthetic process Traceable author statement. Source: Reactome negative regulation of fibroblast migration Inferred from mutant phenotype PubMed 19577615. Source: UniProtKB small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	integral to plasma membrane Traceable author statement PubMed 9169154. Source: ProtInc
Molecular_function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF6	P56537	1	EBI-1052423,EBI-372243
VHL	P40337	1	EBI-1052423,EBI-301246

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 578	578	Hyaluronan synthase 1		PRO_0000197169
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Helical; Name=1; Potential
	Topological domain	47 – 52	6	Extracellular Potential
	Transmembrane	53 – 73	21	Helical; Name=2; Potential
	Topological domain	74 – 399	326	Cytoplasmic Potential
	Transmembrane	400 – 420	21	Helical; Name=3; Potential
	Topological domain	421 – 430	10	Extracellular Potential
	Transmembrane	431 – 451	21	Helical; Name=4; Potential
	Topological domain	452 – 457	6	Cytoplasmic Potential
	Transmembrane	458 – 478	21	Helical; Name=5; Potential
	Topological domain	479 – 497	19	Extracellular Potential
	Transmembrane	498 – 518	21	Helical; Name=6; Potential
	Topological domain	519 – 540	22	Cytoplasmic Potential
	Transmembrane	541 – 561	21	Helical; Name=7; Potential
	Topological domain	562 – 578	17	Extracellular Potential
Natural variations
	Natural variant	14	1	C → R. Ref.1 Ref.2 Corresponds to variant rs7248778 [ dbSNP | Ensembl ].		VAR_047025
Experimental info
	Sequence conflict	1 – 2	2	MR → RS in BAA12351. Ref.1
	Sequence conflict	34	1	G → A in BAA12351. Ref.1
	Sequence conflict	128	1	L → V in BAA12351. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q92839 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 2FE3A44B0D5380FF	FASTA	578	64,832
	10 20 30 40 50 60 MRQQDAPKPT PAACRCSGLA RRVLTIAFAL LILGLMTWAY AAGVPLASDR YGLLAFGLYG 70 80 90 100 110 120 AFLSAHLVAQ SLFAYLEHRR VAAAARGPLD AATARSVALT ISAYQEDPAY LRQCLASARA 130 140 150 160 170 180 LLYPRARLRV LMVVDGNRAE DLYMVDMFRE VFADEDPATY VWDGNYHQPW EPAAAGAVGA 190 200 210 220 230 240 GAYREVEAED PGRLAVEALV RTRRCVCVAQ RWGGKREVMY TAFKALGDSV DYVQVCDSDT 250 260 270 280 290 300 RLDPMALLEL VRVLDEDPRV GAVGGDVRIL NPLDSWVSFL SSLRYWVAFN VERACQSYFH 310 320 330 340 350 360 CVSCISGPLG LYRNNLLQQF LEAWYNQKFL GTHCTFGDDR HLTNRMLSMG YATKYTSRSR 370 380 390 400 410 420 CYSETPSSFL RWLSQQTRWS KSYFREWLYN ALWWHRHHAW MTYEAVVSGL FPFFVAATVL 430 440 450 460 470 480 RLFYAGRPWA LLWVLLCVQG VALAKAAFAA WLRGCLRMVL LSLYAPLYMC GLLPAKFLAL 490 500 510 520 530 540 VTMNQSGWGT SGRRKLAANY VPLLPLALWA LLLLGGLVRS VAHEARADWS GPSRAAEAYH 550 560 570 LAAGAGAYVG YWVAMLTLYW VGVRRLCRRR TGGYRVQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of human hyaluronan synthase." Itano N., Kimata K. Biochem. Biophys. Res. Commun. 222:816-820(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-14. Tissue: Fetal brain.
[2]	"Functional cloning of the cDNA for a human hyaluronan synthase." Shyjan A.M., Heldin P., Butcher E.C., Yoshino T., Briskin M.J. J. Biol. Chem. 271:23395-23399(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-14. Tissue: Lymph node.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84424 mRNA. Translation: BAA12351.1. Different initiation. U59269 mRNA. Translation: AAC50706.1. AC018755 Genomic DNA. Translation: AAF87845.1. CH471135 Genomic DNA. Translation: EAW72038.1.
IPI	IPI00023768.
PIR	JC4812.
RefSeq	NP_001514.2. NM_001523.2.
UniGene	Hs.57697.
3D structure databases
ProteinModelPortal	Q92839.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000222115.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
PTM databases
PhosphoSite	Q92839.
Polymorphism databases
DMDM	209572627.
Proteomic databases
PaxDb	Q92839.
PRIDE	Q92839.
Protocols and materials databases
DNASU	3036.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000222115; ENSP00000222115; ENSG00000105509.
GeneID	3036.
KEGG	hsa:3036.
UCSC	uc002pxn.1. human.
Organism-specific databases
CTD	3036.
GeneCards	GC19M052216.
HGNC	HGNC:4818. HAS1.
MIM	601463. gene.
neXtProt	NX_Q92839.
PharmGKB	PA29194.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1215.
HOGENOM	HOG000112847.
HOVERGEN	HBG000189.
InParanoid	Q92839.
KO	K00752.
OMA	HRCVCVA.
OrthoDB	EOG4KSPJN.
PhylomeDB	Q92839.
Enzyme and pathway databases
BRENDA	2.4.1.212. 2681.
Reactome	REACT_111217. Metabolism. REACT_116125. Disease.
UniPathway	UPA00341.
Gene expression databases
ArrayExpress	Q92839.
Bgee	Q92839.
CleanEx	HS_HAS1.
Genevestigator	Q92839.
GermOnline	ENSG00000105509. Homo sapiens.
Family and domain databases
InterPro	IPR026107. HAS/NodC. [Graphical view]
PANTHER	PTHR22913. PTHR22913. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	3036.
NextBio	12018.
SOURCE	Search...

Entry information

Entry name

HAS1_HUMAN

Accession

Primary (citable) accession number: Q92839
Secondary accession number(s): Q14470, Q9NS49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	October 14, 2008
Last modified:	April 3, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.