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Q92817 (EVPL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Envoplakin
Alternative name(s):
210 kDa cornified envelope precursor protein
210 kDa paraneoplastic pemphigus antigen
p210
Gene names
Name:EVPL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2033 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.

Subunit structure

May form a homodimer or a heterodimer with PPL.

Subcellular location

Cell junctiondesmosome. Cornified envelope. Cytoplasmcytoskeleton. Note: Colocalized with DSP at desmosomes and along intermediate filaments.

Tissue specificity

Exclusively expressed in stratified squamous epithelia.

Induction

During differentiation of epidermal keratinocytes.

Post-translational modification

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other envoplakin molecules, to other proteins such as keratin, desmoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (Ref.5).

Sequence similarities

Belongs to the plakin or cytolinker family.

Contains 7 plectin repeats.

Contains 1 spectrin repeat.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20332033Envoplakin
PRO_0000078147

Regions

Repeat229 – 330102Spectrin
Repeat1185 – 122642Plectin 1
Repeat1678 – 171336Plectin 2
Repeat1818 – 185538Plectin 3
Repeat1856 – 189338Plectin 4
Repeat1894 – 193138Plectin 5
Repeat1932 – 196938Plectin 6
Repeat1970 – 200738Plectin 7
Region1 – 841841Globular 1
Region12 – 28174 X 4 AA tandem repeats of K-G-S-P
Region842 – 1673832Central fibrous rod domain
Region1674 – 2033360Globular 2
Coiled coil845 – 1135291 Potential

Amino acid modifications

Modified residue17991Phosphoserine Ref.7
Lipidation6571Omega-hydroxyceramide glutamate ester Potential
Lipidation8711Omega-hydroxyceramide glutamate ester Probable
Lipidation16071Omega-hydroxyceramide glutamate ester Probable
Cross-link1467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.6

Natural variations

Natural variant491N → S.
Corresponds to variant rs570145 [ dbSNP | Ensembl ].
VAR_024579
Natural variant1681Y → C.
Corresponds to variant rs10445216 [ dbSNP | Ensembl ].
VAR_057698
Natural variant4331Q → R.
Corresponds to variant rs2071192 [ dbSNP | Ensembl ].
VAR_033863
Natural variant18141P → S. Ref.4
Corresponds to variant rs7342883 [ dbSNP | Ensembl ].
VAR_057699

Experimental info

Sequence conflict4441D → E in AAC64662. Ref.1
Sequence conflict4441D → E in AAD00186. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q92817 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 0A994627703393E0

FASTA2,033231,604
        10         20         30         40         50         60 
MFKGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA DQVERDILET 

        70         80         90        100        110        120 
QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD VDKARRLKHP QAEEIEKDIK 

       130        140        150        160        170        180 
QLHERVTQEC AEYRALYEKM VLPPDVGPRV DWARVLEQKQ KQVCAGQYGP GMAELEQQIA 

       190        200        210        220        230        240 
EHNILQKEID AYGQQLRSLV GPDAATIRSQ YRDLLKAASW RGQSLGSLYT HLQGCTRQLS 

       250        260        270        280        290        300 
ALAEQQRRIL QQDWSDLMAD PAGVRREYEH FKQHELLSQE QSVNQLEDDG ERMVELRHPA 

       310        320        330        340        350        360 
VGPIQAHQEA LKMEWQNFLN LCICQETQLQ HVEDYRRFQE EADSVSQTLA KLNSNLDAKY 

       370        380        390        400        410        420 
SPAPGGPPGA PTELLQQLEA EEKRLAVTER ATGDLQRRSR DVAPLPQRRN PPQQPLHVDS 

       430        440        450        460        470        480 
ICDWDSGEVQ LLQGERYKLV DNTDPHAWVV QGPGGETKRA PAACFCIPAP DPDAVARASR 

       490        500        510        520        530        540 
LASELQALKQ KLATVQSRLK ASAVESLRPS QQAPSGSDLA NPQAQKLLTQ MTRLDGDLGQ 

       550        560        570        580        590        600 
IERQVLAWAR APLSRPTPLE DLEGRIHSHE GTAQRLQSLG TEKETAQKEC EAFLSTRPVG 

       610        620        630        640        650        660 
PAALQLPVAL NSVKNKFSDV QVLCSLYGEK AKAALDLERQ IQDADRVIRG FEATLVQEAP 

       670        680        690        700        710        720 
IPAEPGALQE RVSELQRQRR ELLEQQTCVL RLHRALKASE HACAALQNNF QEFCQDLPRQ 

       730        740        750        760        770        780 
QRQVRALTDR YHAVGDQLDL REKVVQDAAL TYQQFKNCKD NLSSWLEHLP RSQVRPSDGP 

       790        800        810        820        830        840 
SQIAYKLQAQ KRLTQEIQSR ERDRATASHL SQALQAALQD YELQADTYRC SLEPTLAVSA 

       850        860        870        880        890        900 
PKRPRVAPLQ ESIQAQEKNL AKAYTEVAAA QQQLLQQLEF ARKMLEKKEL SEDIRRTHDA 

       910        920        930        940        950        960 
KQGSESPAQA GRESEALKAQ LEEERKRVAR VQHELEAQRS QLLQLRTQRP LERLEEKEVV 

       970        980        990       1000       1010       1020 
EFYRDPQLEG SLSRVKAQVE EEGKRRAGLQ ADLEVAAQKV VQLESKRKTM QPHLLTKEVT 

      1030       1040       1050       1060       1070       1080 
QVERDPGLDS QAAQLRIQIQ QLRGEDAVIS ARLEGLKKEL LALEKREVDV KEKVVVKEVV 

      1090       1100       1110       1120       1130       1140 
KVEKNLEMVK AAQALRLQME EDAARRKQAE EAVAKLQARI EDLERAISSV EPKVIVKEVK 

      1150       1160       1170       1180       1190       1200 
KVEQDPGLLQ ESSRLRSLLE EERTKNATLA RELSDLHSKY SVVEKQRPKV QLQERVHEIF 

      1210       1220       1230       1240       1250       1260 
QVDPETEQEI TRLKAKLQEM AGKRSGVEKE VEKLLPDLEV LRAQKPTVEY KEVTQEVVRH 

      1270       1280       1290       1300       1310       1320 
ERSPEVLREI DRLKAQLNEL VNSHGRSQEQ LIRLQGERDE WRRERAKVET KTVSKEVVRH 

      1330       1340       1350       1360       1370       1380 
EKDPVLEKEA ERLRQEVREA AQKRRAAEDA VYELQSKRLL LERRKPEEKV VVQEVVVTQK 

      1390       1400       1410       1420       1430       1440 
DPKLREEHSR LSGSLDEEVG RRRQLELEVQ QLRAGVEEQE GLLSFQEDRS KKLAVERELR 

      1450       1460       1470       1480       1490       1500 
QLTLRIQELE KRPPTVQEKI IMEEVVKLEK DPDLEKSTEA LRWDLDQEKT QVTELNRECK 

      1510       1520       1530       1540       1550       1560 
NLQVQIDVLQ KAKSQEKTIY KEVIRVQKDR VLEDERARVW EMLNRERTAR QAREEEARRL 

      1570       1580       1590       1600       1610       1620 
RERIDRAETL GRTWSREESE LQRARDQADQ ECGRLQQELR ALERQKQQQT LQLQEESKLL 

      1630       1640       1650       1660       1670       1680 
SQKTESERQK AAQRGQELSR LEAAILREKD QIYEKERTLR DLHAKVSREE LSQETQTRET 

      1690       1700       1710       1720       1730       1740 
NLSTKISILE PETGKDMSPY EAYKRGIIDR GQYLQLQELE CDWEEVTTSG PCGEESVLLD 

      1750       1760       1770       1780       1790       1800 
RKSGKQYSIE AALRCRRISK EEYHLYKDGH LPISEFALLV AGETKPSSSL SIGSIISKSP 

      1810       1820       1830       1840       1850       1860 
LASPAPQSTS FFSPSFSLGL GDDSFPIAGI YDTTTDNKCS IKTAVAKNML DPITGQKLLE 

      1870       1880       1890       1900       1910       1920 
AQAATGGIVD LLSRERYSVH KAMERGLIEN TSTQRLLNAQ KAFTGIEDPV TKKRLSVGEA 

      1930       1940       1950       1960       1970       1980 
VQKGWMPRES VLPHLQVQHL TGGLIDPKRT GRIPIQQALL SGMISEELAQ LLQDESSYEK 

      1990       2000       2010       2020       2030 
DLTDPISKER LSYKEAMGRC RKDPLSGLLL LPAALEGYRC YRSASPTVPR SLR 

« Hide

References

« Hide 'large scale' references
[1]"Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin."
Ruhrberg C., Hajibagheri M.A.N., Simon M., Dooley T.P., Watt F.M.
J. Cell Biol. 134:715-729(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Keratinocyte.
[2]"Envoplakin, a possible candidate gene for focal NEPPK/esophageal cancer (TOC): the integration of genetic and physical maps of the TOC region on 17q25."
Risk J.M., Ruhrberg C., Hennies H.-C., Mills H.S., Di Colandrea T., Evans K.E., Ellis A., Watt F.M., Bishop D.T., Spurr N.K., Stevens H.P., Leigh I.M., Reis A., Kelsell D.P., Field J.K.
Genomics 59:234-242(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-1814.
Tissue: Cerebellum.
[5]"Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope."
Marekov L.N., Steinert P.M.
J. Biol. Chem. 273:17763-17770(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: LIPIDATION.
[6]"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1467.
Tissue: Mammary cancer.
[7]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1799, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53786 mRNA. Translation: AAC64662.1.
U72849 expand/collapse EMBL AC list , U72843, U72845, U72846, U72847, U72848 Genomic DNA. Translation: AAD00186.1.
AC040980 Genomic DNA. No translation available.
BC126103 mRNA. Translation: AAI26104.1.
BC126105 mRNA. Translation: AAI26106.1.
CCDSCCDS11737.1.
RefSeqNP_001979.2. NM_001988.2.
UniGeneHs.500635.

3D structure databases

ProteinModelPortalQ92817.
SMRQ92817. Positions 156-488, 1822-2014.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108426. 8 interactions.
IntActQ92817. 1 interaction.
STRING9606.ENSP00000301607.

PTM databases

PhosphoSiteQ92817.

Polymorphism databases

DMDM296439359.

Proteomic databases

MaxQBQ92817.
PaxDbQ92817.
PRIDEQ92817.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301607; ENSP00000301607; ENSG00000167880.
GeneID2125.
KEGGhsa:2125.
UCSCuc002jqi.2. human.

Organism-specific databases

CTD2125.
GeneCardsGC17M074002.
H-InvDBHIX0013602.
HIX0039259.
HGNCHGNC:3503. EVPL.
HPACAB002220.
MIM601590. gene.
neXtProtNX_Q92817.
PharmGKBPA27916.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG251306.
HOVERGENHBG018988.
InParanoidQ92817.
KOK10383.
PhylomeDBQ92817.
TreeFamTF342779.

Gene expression databases

ArrayExpressQ92817.
BgeeQ92817.
CleanExHS_EVPL.
GenevestigatorQ92817.

Family and domain databases

Gene3D3.90.1290.10. 2 hits.
InterProIPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view]
PfamPF00681. Plectin. 5 hits.
[Graphical view]
SMARTSM00250. PLEC. 8 hits.
SM00150. SPEC. 1 hit.
[Graphical view]
SUPFAMSSF75399. SSF75399. 3 hits.
ProtoNetSearch...

Other

ChiTaRSEVPL. human.
GeneWikiEnvoplakin.
GenomeRNAi2125.
NextBio8597.
PROQ92817.
SOURCESearch...

Entry information

Entry nameEVPL_HUMAN
AccessionPrimary (citable) accession number: Q92817
Secondary accession number(s): A0AUV5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM