Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q92817

- EVPL_HUMAN

UniProt

Q92817 - EVPL_HUMAN

Protein

Envoplakin

Gene

EVPL

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (18 May 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.

    GO - Molecular functioni

    1. protein binding, bridging Source: UniProtKB
    2. structural molecule activity Source: UniProtKB

    GO - Biological processi

    1. epidermis development Source: ProtInc
    2. keratinization Source: UniProtKB-KW
    3. keratinocyte differentiation Source: UniProtKB
    4. peptide cross-linking Source: UniProtKB

    Keywords - Biological processi

    Keratinization

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envoplakin
    Alternative name(s):
    210 kDa cornified envelope precursor protein
    210 kDa paraneoplastic pemphigus antigen
    p210
    Gene namesi
    Name:EVPL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:3503. EVPL.

    Subcellular locationi

    Cell junctiondesmosome. Cornified envelope. Cytoplasmcytoskeleton
    Note: Colocalized with DSP at desmosomes and along intermediate filaments.

    GO - Cellular componenti

    1. cornified envelope Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. desmosome Source: UniProtKB-SubCell
    4. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27916.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20332033EnvoplakinPRO_0000078147Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi657 – 6571Omega-hydroxyceramide glutamate esterSequence Analysis
    Lipidationi871 – 8711Omega-hydroxyceramide glutamate ester1 Publication
    Cross-linki1467 – 1467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Lipidationi1607 – 16071Omega-hydroxyceramide glutamate ester1 Publication
    Modified residuei1799 – 17991Phosphoserine1 Publication

    Post-translational modificationi

    Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other envoplakin molecules, to other proteins such as keratin, desmoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).1 Publication

    Keywords - PTMi

    Isopeptide bond, Lipoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ92817.
    PaxDbiQ92817.
    PRIDEiQ92817.

    PTM databases

    PhosphoSiteiQ92817.

    Expressioni

    Tissue specificityi

    Exclusively expressed in stratified squamous epithelia.

    Inductioni

    During differentiation of epidermal keratinocytes.

    Gene expression databases

    ArrayExpressiQ92817.
    BgeeiQ92817.
    CleanExiHS_EVPL.
    GenevestigatoriQ92817.

    Organism-specific databases

    HPAiCAB002220.

    Interactioni

    Subunit structurei

    May form a homodimer or a heterodimer with PPL.

    Protein-protein interaction databases

    BioGridi108426. 8 interactions.
    IntActiQ92817. 1 interaction.
    STRINGi9606.ENSP00000301607.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92817.
    SMRiQ92817. Positions 156-488, 1822-2014.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati229 – 330102SpectrinAdd
    BLAST
    Repeati1185 – 122642Plectin 1Add
    BLAST
    Repeati1678 – 171336Plectin 2Add
    BLAST
    Repeati1818 – 185538Plectin 3Add
    BLAST
    Repeati1856 – 189338Plectin 4Add
    BLAST
    Repeati1894 – 193138Plectin 5Add
    BLAST
    Repeati1932 – 196938Plectin 6Add
    BLAST
    Repeati1970 – 200738Plectin 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 841841Globular 1Add
    BLAST
    Regioni12 – 28174 X 4 AA tandem repeats of K-G-S-PAdd
    BLAST
    Regioni842 – 1673832Central fibrous rod domainAdd
    BLAST
    Regioni1674 – 2033360Globular 2Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili845 – 1135291Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the plakin or cytolinker family.Curated
    Contains 7 plectin repeats.Curated
    Contains 1 spectrin repeat.Curated

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG251306.
    HOVERGENiHBG018988.
    InParanoidiQ92817.
    KOiK10383.
    PhylomeDBiQ92817.
    TreeFamiTF342779.

    Family and domain databases

    Gene3Di3.90.1290.10. 2 hits.
    InterProiIPR001101. Plectin_repeat.
    IPR018159. Spectrin/alpha-actinin.
    [Graphical view]
    PfamiPF00681. Plectin. 5 hits.
    [Graphical view]
    SMARTiSM00250. PLEC. 8 hits.
    SM00150. SPEC. 1 hit.
    [Graphical view]
    SUPFAMiSSF75399. SSF75399. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q92817-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA     50
    DQVERDILET QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD 100
    VDKARRLKHP QAEEIEKDIK QLHERVTQEC AEYRALYEKM VLPPDVGPRV 150
    DWARVLEQKQ KQVCAGQYGP GMAELEQQIA EHNILQKEID AYGQQLRSLV 200
    GPDAATIRSQ YRDLLKAASW RGQSLGSLYT HLQGCTRQLS ALAEQQRRIL 250
    QQDWSDLMAD PAGVRREYEH FKQHELLSQE QSVNQLEDDG ERMVELRHPA 300
    VGPIQAHQEA LKMEWQNFLN LCICQETQLQ HVEDYRRFQE EADSVSQTLA 350
    KLNSNLDAKY SPAPGGPPGA PTELLQQLEA EEKRLAVTER ATGDLQRRSR 400
    DVAPLPQRRN PPQQPLHVDS ICDWDSGEVQ LLQGERYKLV DNTDPHAWVV 450
    QGPGGETKRA PAACFCIPAP DPDAVARASR LASELQALKQ KLATVQSRLK 500
    ASAVESLRPS QQAPSGSDLA NPQAQKLLTQ MTRLDGDLGQ IERQVLAWAR 550
    APLSRPTPLE DLEGRIHSHE GTAQRLQSLG TEKETAQKEC EAFLSTRPVG 600
    PAALQLPVAL NSVKNKFSDV QVLCSLYGEK AKAALDLERQ IQDADRVIRG 650
    FEATLVQEAP IPAEPGALQE RVSELQRQRR ELLEQQTCVL RLHRALKASE 700
    HACAALQNNF QEFCQDLPRQ QRQVRALTDR YHAVGDQLDL REKVVQDAAL 750
    TYQQFKNCKD NLSSWLEHLP RSQVRPSDGP SQIAYKLQAQ KRLTQEIQSR 800
    ERDRATASHL SQALQAALQD YELQADTYRC SLEPTLAVSA PKRPRVAPLQ 850
    ESIQAQEKNL AKAYTEVAAA QQQLLQQLEF ARKMLEKKEL SEDIRRTHDA 900
    KQGSESPAQA GRESEALKAQ LEEERKRVAR VQHELEAQRS QLLQLRTQRP 950
    LERLEEKEVV EFYRDPQLEG SLSRVKAQVE EEGKRRAGLQ ADLEVAAQKV 1000
    VQLESKRKTM QPHLLTKEVT QVERDPGLDS QAAQLRIQIQ QLRGEDAVIS 1050
    ARLEGLKKEL LALEKREVDV KEKVVVKEVV KVEKNLEMVK AAQALRLQME 1100
    EDAARRKQAE EAVAKLQARI EDLERAISSV EPKVIVKEVK KVEQDPGLLQ 1150
    ESSRLRSLLE EERTKNATLA RELSDLHSKY SVVEKQRPKV QLQERVHEIF 1200
    QVDPETEQEI TRLKAKLQEM AGKRSGVEKE VEKLLPDLEV LRAQKPTVEY 1250
    KEVTQEVVRH ERSPEVLREI DRLKAQLNEL VNSHGRSQEQ LIRLQGERDE 1300
    WRRERAKVET KTVSKEVVRH EKDPVLEKEA ERLRQEVREA AQKRRAAEDA 1350
    VYELQSKRLL LERRKPEEKV VVQEVVVTQK DPKLREEHSR LSGSLDEEVG 1400
    RRRQLELEVQ QLRAGVEEQE GLLSFQEDRS KKLAVERELR QLTLRIQELE 1450
    KRPPTVQEKI IMEEVVKLEK DPDLEKSTEA LRWDLDQEKT QVTELNRECK 1500
    NLQVQIDVLQ KAKSQEKTIY KEVIRVQKDR VLEDERARVW EMLNRERTAR 1550
    QAREEEARRL RERIDRAETL GRTWSREESE LQRARDQADQ ECGRLQQELR 1600
    ALERQKQQQT LQLQEESKLL SQKTESERQK AAQRGQELSR LEAAILREKD 1650
    QIYEKERTLR DLHAKVSREE LSQETQTRET NLSTKISILE PETGKDMSPY 1700
    EAYKRGIIDR GQYLQLQELE CDWEEVTTSG PCGEESVLLD RKSGKQYSIE 1750
    AALRCRRISK EEYHLYKDGH LPISEFALLV AGETKPSSSL SIGSIISKSP 1800
    LASPAPQSTS FFSPSFSLGL GDDSFPIAGI YDTTTDNKCS IKTAVAKNML 1850
    DPITGQKLLE AQAATGGIVD LLSRERYSVH KAMERGLIEN TSTQRLLNAQ 1900
    KAFTGIEDPV TKKRLSVGEA VQKGWMPRES VLPHLQVQHL TGGLIDPKRT 1950
    GRIPIQQALL SGMISEELAQ LLQDESSYEK DLTDPISKER LSYKEAMGRC 2000
    RKDPLSGLLL LPAALEGYRC YRSASPTVPR SLR 2033
    Length:2,033
    Mass (Da):231,604
    Last modified:May 18, 2010 - v3
    Checksum:i0A994627703393E0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti444 – 4441D → E in AAC64662. (PubMed:8707850)Curated
    Sequence conflicti444 – 4441D → E in AAD00186. (PubMed:10409435)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti49 – 491N → S.
    Corresponds to variant rs570145 [ dbSNP | Ensembl ].
    VAR_024579
    Natural varianti168 – 1681Y → C.
    Corresponds to variant rs10445216 [ dbSNP | Ensembl ].
    VAR_057698
    Natural varianti433 – 4331Q → R.
    Corresponds to variant rs2071192 [ dbSNP | Ensembl ].
    VAR_033863
    Natural varianti1814 – 18141P → S.1 Publication
    Corresponds to variant rs7342883 [ dbSNP | Ensembl ].
    VAR_057699

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53786 mRNA. Translation: AAC64662.1.
    U72849
    , U72843, U72845, U72846, U72847, U72848 Genomic DNA. Translation: AAD00186.1.
    AC040980 Genomic DNA. No translation available.
    BC126103 mRNA. Translation: AAI26104.1.
    BC126105 mRNA. Translation: AAI26106.1.
    CCDSiCCDS11737.1.
    RefSeqiNP_001979.2. NM_001988.2.
    UniGeneiHs.500635.

    Genome annotation databases

    EnsembliENST00000301607; ENSP00000301607; ENSG00000167880.
    GeneIDi2125.
    KEGGihsa:2125.
    UCSCiuc002jqi.2. human.

    Polymorphism databases

    DMDMi296439359.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U53786 mRNA. Translation: AAC64662.1 .
    U72849
    , U72843 , U72845 , U72846 , U72847 , U72848 Genomic DNA. Translation: AAD00186.1 .
    AC040980 Genomic DNA. No translation available.
    BC126103 mRNA. Translation: AAI26104.1 .
    BC126105 mRNA. Translation: AAI26106.1 .
    CCDSi CCDS11737.1.
    RefSeqi NP_001979.2. NM_001988.2.
    UniGenei Hs.500635.

    3D structure databases

    ProteinModelPortali Q92817.
    SMRi Q92817. Positions 156-488, 1822-2014.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108426. 8 interactions.
    IntActi Q92817. 1 interaction.
    STRINGi 9606.ENSP00000301607.

    PTM databases

    PhosphoSitei Q92817.

    Polymorphism databases

    DMDMi 296439359.

    Proteomic databases

    MaxQBi Q92817.
    PaxDbi Q92817.
    PRIDEi Q92817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000301607 ; ENSP00000301607 ; ENSG00000167880 .
    GeneIDi 2125.
    KEGGi hsa:2125.
    UCSCi uc002jqi.2. human.

    Organism-specific databases

    CTDi 2125.
    GeneCardsi GC17M074002.
    H-InvDB HIX0013602.
    HIX0039259.
    HGNCi HGNC:3503. EVPL.
    HPAi CAB002220.
    MIMi 601590. gene.
    neXtProti NX_Q92817.
    PharmGKBi PA27916.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG251306.
    HOVERGENi HBG018988.
    InParanoidi Q92817.
    KOi K10383.
    PhylomeDBi Q92817.
    TreeFami TF342779.

    Miscellaneous databases

    ChiTaRSi EVPL. human.
    GeneWikii Envoplakin.
    GenomeRNAii 2125.
    NextBioi 8597.
    PROi Q92817.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q92817.
    Bgeei Q92817.
    CleanExi HS_EVPL.
    Genevestigatori Q92817.

    Family and domain databases

    Gene3Di 3.90.1290.10. 2 hits.
    InterProi IPR001101. Plectin_repeat.
    IPR018159. Spectrin/alpha-actinin.
    [Graphical view ]
    Pfami PF00681. Plectin. 5 hits.
    [Graphical view ]
    SMARTi SM00250. PLEC. 8 hits.
    SM00150. SPEC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75399. SSF75399. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin."
      Ruhrberg C., Hajibagheri M.A.N., Simon M., Dooley T.P., Watt F.M.
      J. Cell Biol. 134:715-729(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Keratinocyte.
    2. "Envoplakin, a possible candidate gene for focal NEPPK/esophageal cancer (TOC): the integration of genetic and physical maps of the TOC region on 17q25."
      Risk J.M., Ruhrberg C., Hennies H.-C., Mills H.S., Di Colandrea T., Evans K.E., Ellis A., Watt F.M., Bishop D.T., Spurr N.K., Stevens H.P., Leigh I.M., Reis A., Kelsell D.P., Field J.K.
      Genomics 59:234-242(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-1814.
      Tissue: Cerebellum.
    5. "Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope."
      Marekov L.N., Steinert P.M.
      J. Biol. Chem. 273:17763-17770(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: LIPIDATION.
    6. "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
      Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
      Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1467.
      Tissue: Mammary cancer.
    7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1799, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiEVPL_HUMAN
    AccessioniPrimary (citable) accession number: Q92817
    Secondary accession number(s): A0AUV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3