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Q92817

- EVPL_HUMAN

UniProt

Q92817 - EVPL_HUMAN

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Protein

Envoplakin

Gene

EVPL

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.

GO - Molecular functioni

  1. protein binding, bridging Source: UniProtKB
  2. structural molecule activity Source: UniProtKB

GO - Biological processi

  1. epidermis development Source: ProtInc
  2. keratinization Source: UniProtKB-KW
  3. keratinocyte differentiation Source: UniProtKB
  4. peptide cross-linking Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Keratinization

Names & Taxonomyi

Protein namesi
Recommended name:
Envoplakin
Alternative name(s):
210 kDa cornified envelope precursor protein
210 kDa paraneoplastic pemphigus antigen
p210
Gene namesi
Name:EVPL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:3503. EVPL.

Subcellular locationi

Cell junctiondesmosome. Cornified envelope. Cytoplasmcytoskeleton
Note: Colocalized with DSP at desmosomes and along intermediate filaments.

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cornified envelope Source: UniProtKB
  3. cytoplasm Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27916.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20332033EnvoplakinPRO_0000078147Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi657 – 6571Omega-hydroxyceramide glutamate esterSequence Analysis
Lipidationi871 – 8711Omega-hydroxyceramide glutamate ester1 Publication
Cross-linki1467 – 1467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Lipidationi1607 – 16071Omega-hydroxyceramide glutamate ester1 Publication
Modified residuei1799 – 17991Phosphoserine1 Publication

Post-translational modificationi

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other envoplakin molecules, to other proteins such as keratin, desmoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377).1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ92817.
PaxDbiQ92817.
PRIDEiQ92817.

PTM databases

PhosphoSiteiQ92817.

Expressioni

Tissue specificityi

Exclusively expressed in stratified squamous epithelia.

Inductioni

During differentiation of epidermal keratinocytes.

Gene expression databases

BgeeiQ92817.
CleanExiHS_EVPL.
ExpressionAtlasiQ92817. baseline and differential.
GenevestigatoriQ92817.

Organism-specific databases

HPAiCAB002220.

Interactioni

Subunit structurei

May form a homodimer or a heterodimer with PPL.

Protein-protein interaction databases

BioGridi108426. 13 interactions.
IntActiQ92817. 1 interaction.
STRINGi9606.ENSP00000301607.

Structurei

3D structure databases

ProteinModelPortaliQ92817.
SMRiQ92817. Positions 156-488, 1686-1782, 1822-2014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati229 – 330102SpectrinAdd
BLAST
Repeati1185 – 122642Plectin 1Add
BLAST
Repeati1678 – 171336Plectin 2Add
BLAST
Repeati1818 – 185538Plectin 3Add
BLAST
Repeati1856 – 189338Plectin 4Add
BLAST
Repeati1894 – 193138Plectin 5Add
BLAST
Repeati1932 – 196938Plectin 6Add
BLAST
Repeati1970 – 200738Plectin 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 841841Globular 1Add
BLAST
Regioni12 – 28174 X 4 AA tandem repeats of K-G-S-PAdd
BLAST
Regioni842 – 1673832Central fibrous rod domainAdd
BLAST
Regioni1674 – 2033360Globular 2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili845 – 1135291Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the plakin or cytolinker family.Curated
Contains 7 plectin repeats.Curated
Contains 1 spectrin repeat.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG251306.
GeneTreeiENSGT00760000119163.
HOVERGENiHBG018988.
InParanoidiQ92817.
KOiK10383.
PhylomeDBiQ92817.
TreeFamiTF342779.

Family and domain databases

Gene3Di3.90.1290.10. 2 hits.
InterProiIPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view]
PfamiPF00681. Plectin. 5 hits.
[Graphical view]
SMARTiSM00250. PLEC. 8 hits.
SM00150. SPEC. 1 hit.
[Graphical view]
SUPFAMiSSF75399. SSF75399. 3 hits.

Sequencei

Sequence statusi: Complete.

Q92817-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA
60 70 80 90 100
DQVERDILET QKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD
110 120 130 140 150
VDKARRLKHP QAEEIEKDIK QLHERVTQEC AEYRALYEKM VLPPDVGPRV
160 170 180 190 200
DWARVLEQKQ KQVCAGQYGP GMAELEQQIA EHNILQKEID AYGQQLRSLV
210 220 230 240 250
GPDAATIRSQ YRDLLKAASW RGQSLGSLYT HLQGCTRQLS ALAEQQRRIL
260 270 280 290 300
QQDWSDLMAD PAGVRREYEH FKQHELLSQE QSVNQLEDDG ERMVELRHPA
310 320 330 340 350
VGPIQAHQEA LKMEWQNFLN LCICQETQLQ HVEDYRRFQE EADSVSQTLA
360 370 380 390 400
KLNSNLDAKY SPAPGGPPGA PTELLQQLEA EEKRLAVTER ATGDLQRRSR
410 420 430 440 450
DVAPLPQRRN PPQQPLHVDS ICDWDSGEVQ LLQGERYKLV DNTDPHAWVV
460 470 480 490 500
QGPGGETKRA PAACFCIPAP DPDAVARASR LASELQALKQ KLATVQSRLK
510 520 530 540 550
ASAVESLRPS QQAPSGSDLA NPQAQKLLTQ MTRLDGDLGQ IERQVLAWAR
560 570 580 590 600
APLSRPTPLE DLEGRIHSHE GTAQRLQSLG TEKETAQKEC EAFLSTRPVG
610 620 630 640 650
PAALQLPVAL NSVKNKFSDV QVLCSLYGEK AKAALDLERQ IQDADRVIRG
660 670 680 690 700
FEATLVQEAP IPAEPGALQE RVSELQRQRR ELLEQQTCVL RLHRALKASE
710 720 730 740 750
HACAALQNNF QEFCQDLPRQ QRQVRALTDR YHAVGDQLDL REKVVQDAAL
760 770 780 790 800
TYQQFKNCKD NLSSWLEHLP RSQVRPSDGP SQIAYKLQAQ KRLTQEIQSR
810 820 830 840 850
ERDRATASHL SQALQAALQD YELQADTYRC SLEPTLAVSA PKRPRVAPLQ
860 870 880 890 900
ESIQAQEKNL AKAYTEVAAA QQQLLQQLEF ARKMLEKKEL SEDIRRTHDA
910 920 930 940 950
KQGSESPAQA GRESEALKAQ LEEERKRVAR VQHELEAQRS QLLQLRTQRP
960 970 980 990 1000
LERLEEKEVV EFYRDPQLEG SLSRVKAQVE EEGKRRAGLQ ADLEVAAQKV
1010 1020 1030 1040 1050
VQLESKRKTM QPHLLTKEVT QVERDPGLDS QAAQLRIQIQ QLRGEDAVIS
1060 1070 1080 1090 1100
ARLEGLKKEL LALEKREVDV KEKVVVKEVV KVEKNLEMVK AAQALRLQME
1110 1120 1130 1140 1150
EDAARRKQAE EAVAKLQARI EDLERAISSV EPKVIVKEVK KVEQDPGLLQ
1160 1170 1180 1190 1200
ESSRLRSLLE EERTKNATLA RELSDLHSKY SVVEKQRPKV QLQERVHEIF
1210 1220 1230 1240 1250
QVDPETEQEI TRLKAKLQEM AGKRSGVEKE VEKLLPDLEV LRAQKPTVEY
1260 1270 1280 1290 1300
KEVTQEVVRH ERSPEVLREI DRLKAQLNEL VNSHGRSQEQ LIRLQGERDE
1310 1320 1330 1340 1350
WRRERAKVET KTVSKEVVRH EKDPVLEKEA ERLRQEVREA AQKRRAAEDA
1360 1370 1380 1390 1400
VYELQSKRLL LERRKPEEKV VVQEVVVTQK DPKLREEHSR LSGSLDEEVG
1410 1420 1430 1440 1450
RRRQLELEVQ QLRAGVEEQE GLLSFQEDRS KKLAVERELR QLTLRIQELE
1460 1470 1480 1490 1500
KRPPTVQEKI IMEEVVKLEK DPDLEKSTEA LRWDLDQEKT QVTELNRECK
1510 1520 1530 1540 1550
NLQVQIDVLQ KAKSQEKTIY KEVIRVQKDR VLEDERARVW EMLNRERTAR
1560 1570 1580 1590 1600
QAREEEARRL RERIDRAETL GRTWSREESE LQRARDQADQ ECGRLQQELR
1610 1620 1630 1640 1650
ALERQKQQQT LQLQEESKLL SQKTESERQK AAQRGQELSR LEAAILREKD
1660 1670 1680 1690 1700
QIYEKERTLR DLHAKVSREE LSQETQTRET NLSTKISILE PETGKDMSPY
1710 1720 1730 1740 1750
EAYKRGIIDR GQYLQLQELE CDWEEVTTSG PCGEESVLLD RKSGKQYSIE
1760 1770 1780 1790 1800
AALRCRRISK EEYHLYKDGH LPISEFALLV AGETKPSSSL SIGSIISKSP
1810 1820 1830 1840 1850
LASPAPQSTS FFSPSFSLGL GDDSFPIAGI YDTTTDNKCS IKTAVAKNML
1860 1870 1880 1890 1900
DPITGQKLLE AQAATGGIVD LLSRERYSVH KAMERGLIEN TSTQRLLNAQ
1910 1920 1930 1940 1950
KAFTGIEDPV TKKRLSVGEA VQKGWMPRES VLPHLQVQHL TGGLIDPKRT
1960 1970 1980 1990 2000
GRIPIQQALL SGMISEELAQ LLQDESSYEK DLTDPISKER LSYKEAMGRC
2010 2020 2030
RKDPLSGLLL LPAALEGYRC YRSASPTVPR SLR
Length:2,033
Mass (Da):231,604
Last modified:May 18, 2010 - v3
Checksum:i0A994627703393E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti444 – 4441D → E in AAC64662. (PubMed:8707850)Curated
Sequence conflicti444 – 4441D → E in AAD00186. (PubMed:10409435)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti49 – 491N → S.
Corresponds to variant rs570145 [ dbSNP | Ensembl ].
VAR_024579
Natural varianti168 – 1681Y → C.
Corresponds to variant rs10445216 [ dbSNP | Ensembl ].
VAR_057698
Natural varianti433 – 4331Q → R.
Corresponds to variant rs2071192 [ dbSNP | Ensembl ].
VAR_033863
Natural varianti1814 – 18141P → S.1 Publication
Corresponds to variant rs7342883 [ dbSNP | Ensembl ].
VAR_057699

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53786 mRNA. Translation: AAC64662.1.
U72849
, U72843, U72845, U72846, U72847, U72848 Genomic DNA. Translation: AAD00186.1.
AC040980 Genomic DNA. No translation available.
BC126103 mRNA. Translation: AAI26104.1.
BC126105 mRNA. Translation: AAI26106.1.
CCDSiCCDS11737.1.
RefSeqiNP_001979.2. NM_001988.2.
UniGeneiHs.500635.

Genome annotation databases

EnsembliENST00000301607; ENSP00000301607; ENSG00000167880.
GeneIDi2125.
KEGGihsa:2125.
UCSCiuc002jqi.2. human.

Polymorphism databases

DMDMi296439359.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53786 mRNA. Translation: AAC64662.1 .
U72849
, U72843 , U72845 , U72846 , U72847 , U72848 Genomic DNA. Translation: AAD00186.1 .
AC040980 Genomic DNA. No translation available.
BC126103 mRNA. Translation: AAI26104.1 .
BC126105 mRNA. Translation: AAI26106.1 .
CCDSi CCDS11737.1.
RefSeqi NP_001979.2. NM_001988.2.
UniGenei Hs.500635.

3D structure databases

ProteinModelPortali Q92817.
SMRi Q92817. Positions 156-488, 1686-1782, 1822-2014.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108426. 13 interactions.
IntActi Q92817. 1 interaction.
STRINGi 9606.ENSP00000301607.

PTM databases

PhosphoSitei Q92817.

Polymorphism databases

DMDMi 296439359.

Proteomic databases

MaxQBi Q92817.
PaxDbi Q92817.
PRIDEi Q92817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000301607 ; ENSP00000301607 ; ENSG00000167880 .
GeneIDi 2125.
KEGGi hsa:2125.
UCSCi uc002jqi.2. human.

Organism-specific databases

CTDi 2125.
GeneCardsi GC17M074002.
H-InvDB HIX0013602.
HIX0039259.
HGNCi HGNC:3503. EVPL.
HPAi CAB002220.
MIMi 601590. gene.
neXtProti NX_Q92817.
PharmGKBi PA27916.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG251306.
GeneTreei ENSGT00760000119163.
HOVERGENi HBG018988.
InParanoidi Q92817.
KOi K10383.
PhylomeDBi Q92817.
TreeFami TF342779.

Miscellaneous databases

ChiTaRSi EVPL. human.
GeneWikii Envoplakin.
GenomeRNAii 2125.
NextBioi 8597.
PROi Q92817.
SOURCEi Search...

Gene expression databases

Bgeei Q92817.
CleanExi HS_EVPL.
ExpressionAtlasi Q92817. baseline and differential.
Genevestigatori Q92817.

Family and domain databases

Gene3Di 3.90.1290.10. 2 hits.
InterProi IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
[Graphical view ]
Pfami PF00681. Plectin. 5 hits.
[Graphical view ]
SMARTi SM00250. PLEC. 8 hits.
SM00150. SPEC. 1 hit.
[Graphical view ]
SUPFAMi SSF75399. SSF75399. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin."
    Ruhrberg C., Hajibagheri M.A.N., Simon M., Dooley T.P., Watt F.M.
    J. Cell Biol. 134:715-729(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Keratinocyte.
  2. "Envoplakin, a possible candidate gene for focal NEPPK/esophageal cancer (TOC): the integration of genetic and physical maps of the TOC region on 17q25."
    Risk J.M., Ruhrberg C., Hennies H.-C., Mills H.S., Di Colandrea T., Evans K.E., Ellis A., Watt F.M., Bishop D.T., Spurr N.K., Stevens H.P., Leigh I.M., Reis A., Kelsell D.P., Field J.K.
    Genomics 59:234-242(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-1814.
    Tissue: Cerebellum.
  5. "Ceramides are bound to structural proteins of the human foreskin epidermal cornified cell envelope."
    Marekov L.N., Steinert P.M.
    J. Biol. Chem. 273:17763-17770(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: LIPIDATION.
  6. "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
    Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
    Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1467.
    Tissue: Mammary cancer.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1799, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiEVPL_HUMAN
AccessioniPrimary (citable) accession number: Q92817
Secondary accession number(s): A0AUV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 18, 2010
Last modified: October 29, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3