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Protein

G protein-activated inward rectifier potassium channel 3

Gene

KCNJ9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei150 – 1501Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

  1. G-protein activated inward rectifier potassium channel activity Source: GO_Central

GO - Biological processi

  1. potassium ion import Source: GO_Central
  2. regulation of ion transmembrane transport Source: GO_Central
  3. synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_75831. Activation of G protein gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-activated inward rectifier potassium channel 3
Short name:
GIRK-3
Alternative name(s):
Inward rectifier K(+) channel Kir3.3
Potassium channel, inwardly rectifying subfamily J member 9
Gene namesi
Name:KCNJ9
Synonyms:GIRK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:6270. KCNJ9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757CytoplasmicBy similarityAdd
BLAST
Transmembranei58 – 8225Helical; Name=M1By similarityAdd
BLAST
Topological domaini83 – 10624ExtracellularBy similarityAdd
BLAST
Intramembranei107 – 11812Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei119 – 1257Pore-formingBy similarity
Topological domaini126 – 1349ExtracellularBy similarity
Transmembranei135 – 15622Helical; Name=M2By similarityAdd
BLAST
Topological domaini157 – 393237CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: GO_Central
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30051.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393G protein-activated inward rectifier potassium channel 3PRO_0000154950Add
BLAST

Proteomic databases

PaxDbiQ92806.
PRIDEiQ92806.

PTM databases

PhosphoSiteiQ92806.

Expressioni

Gene expression databases

BgeeiQ92806.
CleanExiHS_KCNJ9.
GenevestigatoriQ92806.

Interactioni

Subunit structurei

Associates with GIRK1 to form a G-protein-activated heteromultimer pore-forming unit. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane (By similarity).By similarity

Protein-protein interaction databases

BioGridi109967. 2 interactions.
STRINGi9606.ENSP00000357067.

Structurei

3D structure databases

ProteinModelPortaliQ92806.
SMRiQ92806. Positions 22-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1256Selectivity filterBy similarity
Motifi390 – 3934PDZ-binding

Domaini

The PDZ-binding motif specifically binds the PDZ domain of SNX27: the specificity for SNX27 is provided by the 2 residues located upstream (Glu-388 and Ser-389) of the PDZ-binding motif.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ92806.
KOiK05002.
OMAiYVEKNGR.
OrthoDBiEOG7XPZ5K.
PhylomeDBiQ92806.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003276. K_chnl_inward-rec_Kir3.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF17. PTHR11767:SF17. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01329. KIR33CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQENAAFSP GQEEPPRRRG RQRYVEKDGR CNVQQGNVRE TYRYLTDLFT
60 70 80 90 100
TLVDLQWRLS LLFFVLAYAL TWLFFGAIWW LIAYGRGDLE HLEDTAWTPC
110 120 130 140 150
VNNLNGFVAA FLFSIETETT IGYGHRVITD QCPEGIVLLL LQAILGSMVN
160 170 180 190 200
AFMVGCMFVK ISQPNKRAAT LVFSSHAVVS LRDGRLCLMF RVGDLRSSHI
210 220 230 240 250
VEASIRAKLI RSRQTLEGEF IPLHQTDLSV GFDTGDDRLF LVSPLVISHE
260 270 280 290 300
IDAASPFWEA SRRALERDDF EIVVILEGMV EATGMTCQAR SSYLVDEVLW
310 320 330 340 350
GHRFTSVLTL EDGFYEVDYA SFHETFEVPT PSCSARELAE AAARLDAHLY
360 370 380 390
WSIPSRLDEK VEEEGAGEGA GGEAGADKEQ NGCLPPPESE SKV
Length:393
Mass (Da):44,020
Last modified:February 6, 2007 - v2
Checksum:iC6F79F80A37B01C9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti366 – 3661A → V.2 Publications
Corresponds to variant rs3001040 [ dbSNP | Ensembl ].
VAR_023568

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52152 mRNA. Translation: AAB07043.1.
AF193615 Genomic DNA. Translation: AAF89098.1.
AL121987 Genomic DNA. Translation: CAI15268.1.
CCDSiCCDS1194.1.
RefSeqiNP_004974.2. NM_004983.2.
XP_006711369.1. XM_006711306.1.
UniGeneiHs.66726.

Genome annotation databases

EnsembliENST00000368088; ENSP00000357067; ENSG00000162728.
GeneIDi3765.
KEGGihsa:3765.
UCSCiuc001fuy.1. human.

Polymorphism databases

DMDMi125987834.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52152 mRNA. Translation: AAB07043.1.
AF193615 Genomic DNA. Translation: AAF89098.1.
AL121987 Genomic DNA. Translation: CAI15268.1.
CCDSiCCDS1194.1.
RefSeqiNP_004974.2. NM_004983.2.
XP_006711369.1. XM_006711306.1.
UniGeneiHs.66726.

3D structure databases

ProteinModelPortaliQ92806.
SMRiQ92806. Positions 22-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109967. 2 interactions.
STRINGi9606.ENSP00000357067.

Chemistry

ChEMBLiCHEMBL3038490.
GuidetoPHARMACOLOGYi436.

PTM databases

PhosphoSiteiQ92806.

Polymorphism databases

DMDMi125987834.

Proteomic databases

PaxDbiQ92806.
PRIDEiQ92806.

Protocols and materials databases

DNASUi3765.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368088; ENSP00000357067; ENSG00000162728.
GeneIDi3765.
KEGGihsa:3765.
UCSCiuc001fuy.1. human.

Organism-specific databases

CTDi3765.
GeneCardsiGC01P160051.
H-InvDBHIX0028866.
HGNCiHGNC:6270. KCNJ9.
MIMi600932. gene.
neXtProtiNX_Q92806.
PharmGKBiPA30051.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG72812.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ92806.
KOiK05002.
OMAiYVEKNGR.
OrthoDBiEOG7XPZ5K.
PhylomeDBiQ92806.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiREACT_25004. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_75831. Activation of G protein gated Potassium channels.

Miscellaneous databases

GeneWikiiKCNJ9.
GenomeRNAii3765.
NextBioi14763.
PROiQ92806.
SOURCEiSearch...

Gene expression databases

BgeeiQ92806.
CleanExiHS_KCNJ9.
GenevestigatoriQ92806.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003276. K_chnl_inward-rec_Kir3.3.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF17. PTHR11767:SF17. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01329. KIR33CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Co-expression of human Kir3 subunits can yield channels with different functional properties."
    Schoots O., Wilson J.M., Ethier N., Bigras E., Hebert T.E., Van Tol H.H.M.
    Cell. Signal. 11:871-883(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-366.
  2. "Genomic structure and expression of human KCNJ9 (Kir3.3/GIRK3)."
    Vaughn J., Wolford J.K., Prochazka M., Permana P.A.
    Biochem. Biophys. Res. Commun. 274:302-309(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-366.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKCNJ9_HUMAN
AccessioniPrimary (citable) accession number: Q92806
Secondary accession number(s): Q5JW75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 6, 2007
Last modified: February 4, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.