Q92804 (RBP56_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA-binding protein-associated factor 2N

Alternative name(s):
68 kDa TATA-binding protein-associated factor
Short name=TAF(II)68
Short name=TAFII68
RNA-binding protein 56

Gene names

Name:	TAF15
Synonyms:	RBP56, TAF2N

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). Ref.12
Subunit structure	Belongs to the RNA polymerase II (Pol II) transcriptional multiprotein complex, together with the TATA-binding protein (TBP) and other TBP-associated factors (TAF(II)s). Binds SF1.
Subcellular location	Nucleus. Cytoplasm. Note: Shuttles from the nucleus to the cytoplasm. Ref.12
Tissue specificity	Ubiquitous. Observed in all fetal and adult tissues.
Post-translational modification	Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Ref.8 Ref.12
Involvement in disease	A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similarities	Belongs to the RRM TET family. Contains 1 RanBP2-type zinc finger. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement
Disease	Proto-oncogene
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
PTM	Acetylation Methylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Traceable author statement Ref.12. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.12. Source: UniProtKB nucleus Inferred from direct assay Ref.12. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q92804-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q92804-2)

The sequence of this isoform differs from the canonical sequence as follows:
     60-62: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	TATA-binding protein-associated factor 2N		PRO_0000081749
Regions
	Domain	234 – 320	87	RRM
	Repeat	407 – 413	7	1
	Repeat	414 – 420	7	2
	Repeat	421 – 429	9	3
	Repeat	430 – 439	10	4
	Repeat	440 – 448	9	5
	Repeat	449 – 457	9	6
	Repeat	458 – 465	8	7
	Repeat	466 – 473	8	8
	Repeat	474 – 481	8	9
	Repeat	482 – 488	7	10
	Repeat	489 – 496	8	11
	Repeat	497 – 503	7	12
	Repeat	504 – 510	7	13
	Repeat	511 – 517	7	14
	Repeat	518 – 524	7	15
	Repeat	525 – 533	9	16
	Repeat	534 – 543	10	17
	Repeat	544 – 551	8	18
	Repeat	552 – 560	9	19
	Repeat	561 – 568	8	20
	Repeat	569 – 575	7	21
	Zinc finger	354 – 385	32	RanBP2-type
	Region	407 – 575	169	21 X approximate tandem repeats of D-R-[S,G](0,3)-G-G-Y-G-G
	Compositional bias	1 – 208	208	Gln/Gly/Ser/Tyr-rich
	Compositional bias	320 – 590	271	Arg/Gly-rich
Amino acid modifications
	Modified residue	206	1	Asymmetric dimethylarginine; by PRMT1 Ref.8 Ref.12
	Modified residue	226	1	Phosphoserine Ref.13
	Modified residue	231	1	Phosphoserine Ref.13
	Modified residue	268	1	N6-acetyllysine Ref.14
	Modified residue	423	1	Phosphoserine Ref.16
	Modified residue	438	1	Phosphoserine Ref.16
	Modified residue	442	1	Phosphoserine Ref.16
	Modified residue	451	1	Phosphoserine Ref.16
	Modified residue	483	1	Dimethylated arginine Ref.8
	Modified residue	528	1	Asymmetric dimethylarginine
	Modified residue	535	1	Asymmetric dimethylarginine
	Modified residue	554	1	Phosphoserine Ref.16
	Modified residue	570	1	Asymmetric dimethylarginine Ref.8
Natural variations
	Alternative sequence	60 – 62	3	Missing in isoform Short.		VSP_005806

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 73D37C171E1E2BCA	FASTA	592	61,830
	10 20 30 40 50 60 MSDSGSYGQS GGEQQSYSTY GNPGSQGYGQ ASQSYSGYGQ TTDSSYGQNY SGYSSYGQSQ 70 80 90 100 110 120 SGYSQSYGGY ENQKQSSYSQ QPYNNQGQQQ NMESSGSQGG RAPSYDQPDY GQQDSYDQQS 130 140 150 160 170 180 GYDQHQGSYD EQSNYDQQHD SYSQNQQSYH SQRENYSHHT QDDRRDVSRY GEDNRGYGGS 190 200 210 220 230 240 QGGGRGRGGY DKDGRGPMTG SSGGDRGGFK NFGGHRDYGP RTDADSESDN SDNNTIFVQG 250 260 270 280 290 300 LGEGVSTDQV GEFFKQIGII KTNKKTGKPM INLYTDKDTG KPKGEATVSF DDPPSAKAAI 310 320 330 340 350 360 DWFDGKEFHG NIIKVSFATR RPEFMRGGGS GGGRRGRGGY RGRGGFQGRG GDPKSGDWVC 370 380 390 400 410 420 PNPSCGNMNF ARRNSCNQCN EPRPEDSRPS GGDFRGRGYG GERGYRGRGG RGGDRGGYGG 430 440 450 460 470 480 DRSGGGYGGD RSSGGGYSGD RSGGGYGGDR SGGGYGGDRG GGYGGDRGGG YGGDRGGGYG 490 500 510 520 530 540 GDRGGYGGDR GGGYGGDRGG YGGDRGGYGG DRGGYGGDRG GYGGDRSRGG YGGDRGGGSG 550 560 570 580 590 YGGDRSGGYG GDRSGGGYGG DRGGGYGGDR GGYGGKMGGR NDYRNDQRNR PY
	Isoform Short [UniParc]. Checksum: 5D24CA4BF574824A	FASTA	589	61,558
	10 20 30 40 50 60 MSDSGSYGQS GGEQQSYSTY GNPGSQGYGQ ASQSYSGYGQ TTDSSYGQNY SGYSSYGQSY 70 80 90 100 110 120 SQSYGGYENQ KQSSYSQQPY NNQGQQQNME SSGSQGGRAP SYDQPDYGQQ DSYDQQSGYD 130 140 150 160 170 180 QHQGSYDEQS NYDQQHDSYS QNQQSYHSQR ENYSHHTQDD RRDVSRYGED NRGYGGSQGG 190 200 210 220 230 240 GRGRGGYDKD GRGPMTGSSG GDRGGFKNFG GHRDYGPRTD ADSESDNSDN NTIFVQGLGE 250 260 270 280 290 300 GVSTDQVGEF FKQIGIIKTN KKTGKPMINL YTDKDTGKPK GEATVSFDDP PSAKAAIDWF 310 320 330 340 350 360 DGKEFHGNII KVSFATRRPE FMRGGGSGGG RRGRGGYRGR GGFQGRGGDP KSGDWVCPNP 370 380 390 400 410 420 SCGNMNFARR NSCNQCNEPR PEDSRPSGGD FRGRGYGGER GYRGRGGRGG DRGGYGGDRS 430 440 450 460 470 480 GGGYGGDRSS GGGYSGDRSG GGYGGDRSGG GYGGDRGGGY GGDRGGGYGG DRGGGYGGDR 490 500 510 520 530 540 GGYGGDRGGG YGGDRGGYGG DRGGYGGDRG GYGGDRGGYG GDRSRGGYGG DRGGGSGYGG 550 560 570 580 DRSGGYGGDR SGGGYGGDRG GGYGGDRGGY GGKMGGRNDY RNDQRNRPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins." Morohoshi F., Arai K., Takahashi E., Tanigami A., Ohki M. Genomics 38:51-57(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]	"hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II." Bertolotti A., Lutz Y., Heard D.J., Chambon P., Tora L. EMBO J. 15:5022-5031(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), PROTEIN SEQUENCE OF 282-297 AND 307-320.
[3]	"Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes." Morohoshi F., Ootsuka Y., Arai K., Ichikawa H., Mitani S., Munakata N., Ohki M. Gene 221:191-198(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
[4]	NIEHS SNPs program Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
[6]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 75-101; 196-210; 284-306; 476-490 AND 563-576, METHYLATION AT ARG-206; ARG-483 AND ARG-570, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[9]	"The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription." Zhang D., Paley A.J., Childs G. J. Biol. Chem. 273:18086-18091(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SF1.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"PRMT1 mediated methylation of TAF15 is required for its positive gene regulatory function." Jobert L., Argentini M., Tora L. Exp. Cell Res. 315:1273-1286(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, METHYLATION AT ARG-206 BY PRMT1, MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226 AND SER-231, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-268, MASS SPECTROMETRY.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-438; SER-442; SER-451 AND SER-554, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51334 mRNA. Translation: AAC50932.1. X98893 mRNA. Translation: CAA67398.1. AB010067 Genomic DNA. Translation: BAA33811.1. AB010067 Genomic DNA. Translation: BAA33812.1. AY197697 Genomic DNA. Translation: AAO13485.1. AK313223 mRNA. Translation: BAG36034.1. AC015849 Genomic DNA. No translation available. CH471147 Genomic DNA. Translation: EAW80124.1.
IPI	IPI00020194. IPI00294426.
PIR	S71954.
RefSeq	NP_003478.1. NM_003487.3. NP_631961.1. NM_139215.2.
UniGene	Hs.402752.
3D structure databases
ProteinModelPortal	Q92804.
SMR	Q92804. Positions 232-319.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92804. 16 interactions.
STRING	9606.ENSP00000309558.
PTM databases
PhosphoSite	Q92804.
Polymorphism databases
DMDM	8928305.
Proteomic databases
PaxDb	Q92804.
PRIDE	Q92804.
Protocols and materials databases
DNASU	8148.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311979; ENSP00000309558; ENSG00000172660. ENST00000588240; ENSP00000466950; ENSG00000172660.
GeneID	8148.
KEGG	hsa:8148.
UCSC	uc002hkc.3. human. uc002hkd.3. human.
Organism-specific databases
CTD	8148.
GeneCards	GC17P034136.
HGNC	HGNC:11547. TAF15.
HPA	HPA052059.
MIM	601574. gene.
neXtProt	NX_Q92804.
PharmGKB	PA36322.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG240581.
HOGENOM	HOG000038010.
HOVERGEN	HBG005755.
InParanoid	Q92804.
KO	K14651.
OMA	YGNQGSQ.
OrthoDB	EOG4MW872.
PhylomeDB	Q92804.
Gene expression databases
ArrayExpress	Q92804.
Bgee	Q92804.
CleanEx	HS_TAF15.
Genevestigator	Q92804.
GermOnline	ENSG00000172660. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR001876. Znf_RanBP2. [Graphical view]
Pfam	PF00076. RRM_1. 1 hit. PF00641. zf-RanBP. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. SM00547. ZnF_RBZ. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TAF15. human.
GenomeRNAi	8148.
NextBio	30819.
PMAP-CutDB	Q92804.
SOURCE	Search...

Entry information

Entry name

RBP56_HUMAN

Accession

Primary (citable) accession number: Q92804
Secondary accession number(s): B2R837, Q92751

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 140 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.