Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q92804 (RBP56_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 153. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TATA-binding protein-associated factor 2N
Alternative name(s):
68 kDa TATA-binding protein-associated factor
Short name=TAF(II)68
Short name=TAFII68
RNA-binding protein 56
Gene names
Name:TAF15
Synonyms:RBP56, TAF2N
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). Ref.13

Subunit structure

Belongs to the RNA polymerase II (Pol II) transcriptional multiprotein complex, together with the TATA-binding protein (TBP) and other TBP-associated factors (TAF(II)s). Binds SF1.

Subcellular location

Nucleus. Cytoplasm. Note: Shuttles from the nucleus to the cytoplasm. Ref.13

Tissue specificity

Ubiquitous. Observed in all fetal and adult tissues.

Post-translational modification

Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. Ref.9 Ref.13

ADP-ribosylated during genotoxic stress.

Involvement in disease

A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.

Sequence similarities

Belongs to the RRM TET family.

Contains 1 RanBP2-type zinc finger.

Contains 1 RRM (RNA recognition motif) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FUSP356375EBI-2255091,EBI-400434

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q92804-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q92804-2)

The sequence of this isoform differs from the canonical sequence as follows:
     60-62: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 592592TATA-binding protein-associated factor 2N
PRO_0000081749

Regions

Domain234 – 32087RRM
Repeat407 – 41371
Repeat414 – 42072
Repeat421 – 42993
Repeat430 – 439104
Repeat440 – 44895
Repeat449 – 45796
Repeat458 – 46587
Repeat466 – 47388
Repeat474 – 48189
Repeat482 – 488710
Repeat489 – 496811
Repeat497 – 503712
Repeat504 – 510713
Repeat511 – 517714
Repeat518 – 524715
Repeat525 – 533916
Repeat534 – 5431017
Repeat544 – 551818
Repeat552 – 560919
Repeat561 – 568820
Repeat569 – 575721
Zinc finger354 – 38532RanBP2-type
Region407 – 57516921 X approximate tandem repeats of D-R-[S,G](0,3)-G-G-Y-G-G
Compositional bias1 – 208208Gln/Gly/Ser/Tyr-rich
Compositional bias320 – 590271Arg/Gly-rich

Amino acid modifications

Modified residue2061Asymmetric dimethylarginine; by PRMT1 Ref.9 Ref.13
Modified residue2261Phosphoserine Ref.14
Modified residue2311Phosphoserine Ref.14
Modified residue2681N6-acetyllysine Ref.15
Modified residue4231Phosphoserine Ref.17
Modified residue4381Phosphoserine Ref.17
Modified residue4421Phosphoserine Ref.17
Modified residue4511Phosphoserine Ref.17
Modified residue4831Dimethylated arginine Ref.9
Modified residue5281Asymmetric dimethylarginine; by PRMT1 Ref.13
Modified residue5351Asymmetric dimethylarginine; by PRMT1 Ref.13
Modified residue5541Phosphoserine Ref.17
Modified residue5701Asymmetric dimethylarginine Ref.9 Ref.13
Modified residue5701Asymmetric dimethylarginine; by PRMT1 Ref.9 Ref.13

Natural variations

Alternative sequence60 – 623Missing in isoform Short.
VSP_005806

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 73D37C171E1E2BCA

FASTA59261,830
        10         20         30         40         50         60 
MSDSGSYGQS GGEQQSYSTY GNPGSQGYGQ ASQSYSGYGQ TTDSSYGQNY SGYSSYGQSQ 

        70         80         90        100        110        120 
SGYSQSYGGY ENQKQSSYSQ QPYNNQGQQQ NMESSGSQGG RAPSYDQPDY GQQDSYDQQS 

       130        140        150        160        170        180 
GYDQHQGSYD EQSNYDQQHD SYSQNQQSYH SQRENYSHHT QDDRRDVSRY GEDNRGYGGS 

       190        200        210        220        230        240 
QGGGRGRGGY DKDGRGPMTG SSGGDRGGFK NFGGHRDYGP RTDADSESDN SDNNTIFVQG 

       250        260        270        280        290        300 
LGEGVSTDQV GEFFKQIGII KTNKKTGKPM INLYTDKDTG KPKGEATVSF DDPPSAKAAI 

       310        320        330        340        350        360 
DWFDGKEFHG NIIKVSFATR RPEFMRGGGS GGGRRGRGGY RGRGGFQGRG GDPKSGDWVC 

       370        380        390        400        410        420 
PNPSCGNMNF ARRNSCNQCN EPRPEDSRPS GGDFRGRGYG GERGYRGRGG RGGDRGGYGG 

       430        440        450        460        470        480 
DRSGGGYGGD RSSGGGYSGD RSGGGYGGDR SGGGYGGDRG GGYGGDRGGG YGGDRGGGYG 

       490        500        510        520        530        540 
GDRGGYGGDR GGGYGGDRGG YGGDRGGYGG DRGGYGGDRG GYGGDRSRGG YGGDRGGGSG 

       550        560        570        580        590 
YGGDRSGGYG GDRSGGGYGG DRGGGYGGDR GGYGGKMGGR NDYRNDQRNR PY 

« Hide

Isoform Short [UniParc].

Checksum: 5D24CA4BF574824A
Show »

FASTA58961,558

References

« Hide 'large scale' references
[1]"Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins."
Morohoshi F., Arai K., Takahashi E., Tanigami A., Ohki M.
Genomics 38:51-57(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]"hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II."
Bertolotti A., Lutz Y., Heard D.J., Chambon P., Tora L.
EMBO J. 15:5022-5031(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), PROTEIN SEQUENCE OF 282-297 AND 307-320.
[3]"Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes."
Morohoshi F., Ootsuka Y., Arai K., Ichikawa H., Mitani S., Munakata N., Ohki M.
Gene 221:191-198(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS LONG AND SHORT).
[4]NIEHS SNPs program
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS SHORT AND LONG).
Tissue: Tongue.
[6]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Testis.
[9]Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 75-101; 196-210; 284-306; 476-490 AND 563-576, METHYLATION AT ARG-206; ARG-483 AND ARG-570, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Ovarian carcinoma.
[10]"The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription."
Zhang D., Paley A.J., Childs G.
J. Biol. Chem. 273:18086-18091(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SF1.
[11]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[12]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"PRMT1 mediated methylation of TAF15 is required for its positive gene regulatory function."
Jobert L., Argentini M., Tora L.
Exp. Cell Res. 315:1273-1286(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, METHYLATION AT ARG-206; ARG-528; ARG-535 AND ARG-570 BY PRMT1, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[14]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226 AND SER-231, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[15]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[17]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423; SER-438; SER-442; SER-451 AND SER-554, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"Proteome-wide identification of poly(ADP-Ribosyl)ation targets in different genotoxic stress responses."
Jungmichel S., Rosenthal F., Altmeyer M., Lukas J., Hottiger M.O., Nielsen M.L.
Mol. Cell 52:272-285(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ADP-RIBOSYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51334 mRNA. Translation: AAC50932.1.
X98893 mRNA. Translation: CAA67398.1.
AB010067 Genomic DNA. Translation: BAA33811.1.
AB010067 Genomic DNA. Translation: BAA33812.1.
AY197697 Genomic DNA. Translation: AAO13485.1.
AK313223 mRNA. Translation: BAG36034.1.
AK314194 mRNA. Translation: BAG36873.1.
AC015849 Genomic DNA. No translation available.
CH471147 Genomic DNA. Translation: EAW80124.1.
CH471147 Genomic DNA. Translation: EAW80125.1.
BC046099 mRNA. Translation: AAH46099.2.
CCDSCCDS32623.1. [Q92804-1]
CCDS59279.1. [Q92804-2]
PIRS71954.
RefSeqNP_003478.1. NM_003487.3. [Q92804-2]
NP_631961.1. NM_139215.2. [Q92804-1]
UniGeneHs.402752.

3D structure databases

ProteinModelPortalQ92804.
SMRQ92804. Positions 232-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113807. 56 interactions.
DIPDIP-52760N.
IntActQ92804. 21 interactions.
MINTMINT-3049394.
STRING9606.ENSP00000309558.

PTM databases

PhosphoSiteQ92804.

Polymorphism databases

DMDM8928305.

Proteomic databases

MaxQBQ92804.
PaxDbQ92804.
PRIDEQ92804.

Protocols and materials databases

DNASU8148.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000311979; ENSP00000309558; ENSG00000172660. [Q92804-2]
ENST00000588240; ENSP00000466950; ENSG00000172660. [Q92804-1]
ENST00000604841; ENSP00000474609; ENSG00000270647. [Q92804-2]
ENST00000605844; ENSP00000474096; ENSG00000270647. [Q92804-1]
GeneID8148.
KEGGhsa:8148.
UCSCuc002hkc.4. human. [Q92804-2]
uc002hkd.4. human. [Q92804-1]

Organism-specific databases

CTD8148.
GeneCardsGC17P034136.
HGNCHGNC:11547. TAF15.
HPAHPA052059.
MIM601574. gene.
neXtProtNX_Q92804.
Orphanet803. Amyotrophic lateral sclerosis.
209916. Extraskeletal myxoid chondrosarcoma.
PharmGKBPA36322.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240581.
HOGENOMHOG000038010.
HOVERGENHBG005755.
InParanoidQ92804.
KOK14651.
OMAQKQSSYS.
OrthoDBEOG7DZ8N7.
PhylomeDBQ92804.

Gene expression databases

ArrayExpressQ92804.
BgeeQ92804.
CleanExHS_TAF15.
GenevestigatorQ92804.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
4.10.1060.10. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTAF15. human.
GeneWikiTAF15.
GenomeRNAi8148.
NextBio30819.
PMAP-CutDBQ92804.
PROQ92804.
SOURCESearch...

Entry information

Entry nameRBP56_HUMAN
AccessionPrimary (citable) accession number: Q92804
Secondary accession number(s): B2R837, Q86X94, Q92751
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM