Q92802 (N42L2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD4-binding protein 2-like 2

Alternative name(s):
Phosphonoformate immuno-associated protein 5

Gene names

Name:	N4BP2L2
Synonyms:	CG005, PFAAP5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9
Sequence caution	The sequence AAI31632.1 differs from that shown. Reason: Contaminating sequence. Sequence of unknown origin in the N-terminal part. The sequence BP361938 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92802-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92802-2)

The sequence of this isoform differs from the canonical sequence as follows:
     567-583: WGGSLGSHNRVCVTNNH → FDRKLKRWEE...SVEKRQKKIG

Note: No experimental confirmation available.

Isoform 3 (identifier: Q92802-3)

The sequence of this isoform differs from the canonical sequence as follows:
     8-32: GKFLGPREEVTSEPRCKKLKSTTES → EFCLVRIVMALCSALMTIFTIKMGT
     33-461: Missing.
     566-583: RWGGSLGSHNRVCVTNNH → RERVLKKTGH...SVEKRQKKIG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	NEDD4-binding protein 2-like 2		PRO_0000299026
Regions
	Coiled coil	162 – 197	36	Potential
	Compositional bias	558 – 561	4	Poly-Pro
Amino acid modifications
	Modified residue	199	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	8 – 32	25	GKFLG…STTES → EFCLVRIVMALCSALMTIFT IKMGT in isoform 3.		VSP_041337
	Alternative sequence	33 – 461	429	Missing in isoform 3.		VSP_041338
	Alternative sequence	566 – 583	18	RWGGS…VTNNH → RERVLKKTGHRLSKTKQKRN RKRNKKQNSQNRIMEENSLE FLSDLTPGDQDPSQSEEEDI EKTRRESEYPFIDGLQNEVG DFVTGYKEKRWKNKDPKDSF QNVMSIVELDNTPKNYLSKE GDNLFVSLLLRPNEISVTCP ILTQNLSCVTTDDCSGMKVE KHIRNRHTIALDTQDLSAET SCLFMKKREIVDKNLSHEPI LCHQHGIRMSDKVLREEQVY TTKINHWAFFTTNLSDEDLQ LGSDRQPYFGSWPAGPHKFI CEQRPKKDRACKLAGPDSRG QWIQMIFTSVAASEPGNNPE ILTDKLLIGNEDFSPPPETM DSFIETNLFRSCLPQPDIPK NALESTKNKKRRKKRIFNLV PNFDLLGQSRIGVKEREKCD LLTKNHGLKITLGEEKDRIS ERNSEEENKQKLMTFDHHPL WFYLDIIKATPLNIDGQRYS HCLSFNRLRCSASLYKNYIP SFVLHNLSSIWKPSFTNKKL FLTFESQTRVGNKLNDAGFI SPEILHSHPDTSCSLGVTSD FHFLNERFDRKLKRWEEPKE LPAEDSQDLTSTDYRSLELP LSQGFAFQLVKLFGSPGVPM ESLLPDDYVVPLDWKTLKMI YLQWKMSVEKRQKKIG in isoform 3.		VSP_041339
	Alternative sequence	567 – 583	17	WGGSL…VTNNH → FDRKLKRWEEPKELPAEDSQ DLTSTDYRSLELPLSQGFAF QLVKLFGSPGVPMESLLPDD YVVPLDWKTLKMIYLQWKMS VEKRQKKIG in isoform 2.		VSP_027518
	Natural variant	272	1	Y → H. Corresponds to variant rs34062461 [ dbSNP | Ensembl ].		VAR_034765

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 318DC4D81CD0FF2A	FASTA	583	67,459
	10 20 30 40 50 60 MSYGEIEGKF LGPREEVTSE PRCKKLKSTT ESYVFHNHSN ADFHRIQEKT GNDWVPVTII 70 80 90 100 110 120 DVRGHSYLQE NKIKTTDLHR PLHDEMPGNR PDVIESIDSQ VLQEARPPLV SADDEIYSTS 130 140 150 160 170 180 KAFIGPIYKP PEKKKRNEGR NEAHVLNGIN DRGGQKEKQK FNSEKSEIDN ELFQFYKEIE 190 200 210 220 230 240 ELEKEKDGFE NSCKESEPSQ EQFVPFYEGH NNGLLKPDEE KKDLSNKAMP SHCDYQQNLG 250 260 270 280 290 300 NEPDKYPCNG QVIPTFCDTS FTSFRPEWQS VYPFIVPYGP PLPSLNYHLN IQRFSGPPNP 310 320 330 340 350 360 PSNIFQAQDD SQIQNGYYVN NCHVNWNCMT FDQNNEYTDC SENRSSVHPS GNGCSMQDRY 370 380 390 400 410 420 VSNGFCEVRE RCWKDHCMDK HNGTDRFVNQ QFQEEKLNKL QKLLILLRGL PGSGKTTLSR 430 440 450 460 470 480 ILLGQNRDGI VFSTDDYFHH QDGYRYNVNQ LGDAHDWNQN RAKQAIDQGR SPVIIDNTNI 490 500 510 520 530 540 QAWEMKPYVE VAIGKGYRVE FHEPETWWKF DPEELEKRNK HGVSRKKIAQ MLDRYEYQMS 550 560 570 580 ISIVMNSVEP SHKSTQRPPP PQGRQRWGGS LGSHNRVCVT NNH
	Isoform 2 [UniParc]. Checksum: 6CD19BF77B1925BC	FASTA	655	76,082
	10 20 30 40 50 60 MSYGEIEGKF LGPREEVTSE PRCKKLKSTT ESYVFHNHSN ADFHRIQEKT GNDWVPVTII 70 80 90 100 110 120 DVRGHSYLQE NKIKTTDLHR PLHDEMPGNR PDVIESIDSQ VLQEARPPLV SADDEIYSTS 130 140 150 160 170 180 KAFIGPIYKP PEKKKRNEGR NEAHVLNGIN DRGGQKEKQK FNSEKSEIDN ELFQFYKEIE 190 200 210 220 230 240 ELEKEKDGFE NSCKESEPSQ EQFVPFYEGH NNGLLKPDEE KKDLSNKAMP SHCDYQQNLG 250 260 270 280 290 300 NEPDKYPCNG QVIPTFCDTS FTSFRPEWQS VYPFIVPYGP PLPSLNYHLN IQRFSGPPNP 310 320 330 340 350 360 PSNIFQAQDD SQIQNGYYVN NCHVNWNCMT FDQNNEYTDC SENRSSVHPS GNGCSMQDRY 370 380 390 400 410 420 VSNGFCEVRE RCWKDHCMDK HNGTDRFVNQ QFQEEKLNKL QKLLILLRGL PGSGKTTLSR 430 440 450 460 470 480 ILLGQNRDGI VFSTDDYFHH QDGYRYNVNQ LGDAHDWNQN RAKQAIDQGR SPVIIDNTNI 490 500 510 520 530 540 QAWEMKPYVE VAIGKGYRVE FHEPETWWKF DPEELEKRNK HGVSRKKIAQ MLDRYEYQMS 550 560 570 580 590 600 ISIVMNSVEP SHKSTQRPPP PQGRQRFDRK LKRWEEPKEL PAEDSQDLTS TDYRSLELPL 610 620 630 640 650 SQGFAFQLVK LFGSPGVPME SLLPDDYVVP LDWKTLKMIY LQWKMSVEKR QKKIG
	Isoform 3 [UniParc]. Checksum: AA8CC83570FF4F1A	FASTA	752	87,169
	10 20 30 40 50 60 MSYGEIEEFC LVRIVMALCS ALMTIFTIKM GTAKQAIDQG RSPVIIDNTN IQAWEMKPYV 70 80 90 100 110 120 EVAIGKGYRV EFHEPETWWK FDPEELEKRN KHGVSRKKIA QMLDRYEYQM SISIVMNSVE 130 140 150 160 170 180 PSHKSTQRPP PPQGRQRERV LKKTGHRLSK TKQKRNRKRN KKQNSQNRIM EENSLEFLSD 190 200 210 220 230 240 LTPGDQDPSQ SEEEDIEKTR RESEYPFIDG LQNEVGDFVT GYKEKRWKNK DPKDSFQNVM 250 260 270 280 290 300 SIVELDNTPK NYLSKEGDNL FVSLLLRPNE ISVTCPILTQ NLSCVTTDDC SGMKVEKHIR 310 320 330 340 350 360 NRHTIALDTQ DLSAETSCLF MKKREIVDKN LSHEPILCHQ HGIRMSDKVL REEQVYTTKI 370 380 390 400 410 420 NHWAFFTTNL SDEDLQLGSD RQPYFGSWPA GPHKFICEQR PKKDRACKLA GPDSRGQWIQ 430 440 450 460 470 480 MIFTSVAASE PGNNPEILTD KLLIGNEDFS PPPETMDSFI ETNLFRSCLP QPDIPKNALE 490 500 510 520 530 540 STKNKKRRKK RIFNLVPNFD LLGQSRIGVK EREKCDLLTK NHGLKITLGE EKDRISERNS 550 560 570 580 590 600 EEENKQKLMT FDHHPLWFYL DIIKATPLNI DGQRYSHCLS FNRLRCSASL YKNYIPSFVL 610 620 630 640 650 660 HNLSSIWKPS FTNKKLFLTF ESQTRVGNKL NDAGFISPEI LHSHPDTSCS LGVTSDFHFL 670 680 690 700 710 720 NERFDRKLKR WEEPKELPAE DSQDLTSTDY RSLELPLSQG FAFQLVKLFG SPGVPMESLL 730 740 750 PDDYVVPLDW KTLKMIYLQW KMSVEKRQKK IG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Generation of an integrated transcription map of the BRCA2 region on chromosome 13q12-q13." Couch F.J., Rommens J.M., Neuhausen S.L., Belanger C., Dumont M., Kenneth A., Bell R., Berry S., Bogden R., Cannon-Albright L., Farid L., Frye C., Hattier T., Janecki T., Jiang P., Kehrer R., Leblanc J.F., McArthur-Morrison J. , McSweeney D., Miki Y., Peng Y., Samson C., Schroeder M., Snyder S.C., Stringfellow M., Stroup C., Swedlund B., Swensen J., Teng D., Thakur S., Tran T., Tranchant M., Welver-Feldhaus J., Wong A.K.C., Shizuya H., Labrie F., Skolnick M.H., Goldgar D.E., Kamb A., Weber B.L., Tavtigian S.V., Simard J. Genomics 36:86-99(1996) [PubMed: 8812419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Screening and cloning of a new immuno-associated gene regulated by phosphonoformate." Liu Y., Cheng J., Lu Y. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	Rhodes S. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 434-583 (ISOFORM 2). Tissue: Colon.
[8]	"Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." Kimura K., Wakamatsu A., Suzuki Y., Ota T., Nishikawa T., Yamashita R., Yamamoto J., Sekine M., Tsuritani K., Wakaguri H., Ishii S., Sugiyama T., Saito K., Isono Y., Irie R., Kushida N., Yoneyama T., Otsuka R. , Kanda K., Yokoi T., Kondo H., Wagatsuma M., Murakawa K., Ishida S., Ishibashi T., Takahashi-Fujii A., Tanase T., Nagai K., Kikuchi H., Nakai K., Isogai T., Sugano S. Genome Res. 16:55-65(2006) [PubMed: 16344560] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[9]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50532 mRNA. Translation: AAC50875.1. AF530063 mRNA. Translation: AAQ09947.1. AL049783 mRNA. Translation: CAB42441.1. BT007172 mRNA. Translation: AAP35836.1. AL353665 Genomic DNA. Translation: CAI11042.1. CH471075 Genomic DNA. Translation: EAX08514.1. BC010643 mRNA. Translation: AAH10643.1. BC131631 mRNA. Translation: AAI31632.1. Sequence problems. BP361938 mRNA. No translation available.
IPI	IPI00007093. IPI00011822. IPI00856073.
RefSeq	NP_055702.1. NM_014887.2.
UniGene	Hs.507680.
3D structure databases
ProteinModelPortal	Q92802.
SMR	Q92802. Positions 402-541.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92802. 17 interactions.
STRING	Q92802.
PTM databases
PhosphoSite	Q92802.
Polymorphism databases
DMDM	74751659.
Proteomic databases
PRIDE	Q92802.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000267068; ENSP00000267068; ENSG00000244754.
GeneID	10443.
KEGG	hsa:10443.
UCSC	uc001uuk.2. human.
Organism-specific databases
CTD	10443.
GeneCards	GC13M033007.
HGNC	HGNC:26916. N4BP2L2.
HPA	HPA039434.
neXtProt	NX_Q92802.
PharmGKB	PA162396632.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00510000049402.
HOVERGEN	HBG059710.
Gene expression databases
ArrayExpress	Q92802.
Bgee	Q92802.
CleanEx	HS_N4BP2L2.
Genevestigator	Q92802.
Family and domain databases
ProtoNet	Search...
Other
NextBio	39577.

Entry information

Entry name

N42L2_HUMAN

Accession

Primary (citable) accession number: Q92802
Secondary accession number(s): A3KME8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.