Q927Z0 (CLS_LISIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cardiolipin synthase
Short name=CL synthase
EC=2.7.8.-

Gene names

Name:	cls
Ordered Locus Names:	lin2646

Organism

Listeria innocua serovar 6a (strain CLIP 11262) [Complete proteome] [HAMAP]

Taxonomic identifier

272626 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria innocua

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol By similarity. HAMAP-Rule MF_00190
Catalytic activity	2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol. HAMAP-Rule MF_00190
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00190.
Sequence similarities	Belongs to the phospholipase D family. Cardiolipin synthase subfamily. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cardiolipin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cardiolipin synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Cardiolipin synthase HAMAP-Rule MF_00190		PRO_0000201256
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	34 – 54	21	Helical; Potential
	Domain	217 – 244	28	PLD phosphodiesterase 1
	Domain	395 – 422	28	PLD phosphodiesterase 2
Sites
	Active site	222	1	Potential
	Active site	224	1	Potential
	Active site	229	1	Potential
	Active site	400	1	Potential
	Active site	402	1	Potential
	Active site	407	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q927Z0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A0A3F18B99922EC7	FASTA	482	55,462
	10 20 30 40 50 60 MGLLAYLLVI LLILNVFFAA VTVFLERRDT SATWAWLLVL TFVPIFGFII YLIFGRKLSG 70 80 90 100 110 120 KKIFDWKGQE KIGIQESTAN QIEMIRQKEF PFSDPNVKKH RDLIYLLLVN DGAILTQDNE 130 140 150 160 170 180 VELFIDGHEK FDALIADIEK AKDHIHLIYY IFHSDELGNR LMRVLERKAA EGLNVKIIYD 190 200 210 220 230 240 AMGSRTTKKS FFRTFEKNGG LVRPFFPSKL PLINFRLNYR NHRKLAIIDG DVGYIGGFNI 250 260 270 280 290 300 GDEYLGRSKK FGYWRDTHLR VHGKAVYAMQ TRFIMDWNSA SSTHKIDYKA RYFPTFHGKG 310 320 330 340 350 360 HTSMQIVSSG PDSEWQQIKN GYIKMINAAK KTIYLQSPYF IPDASLLEAI KIAALSGVDV 370 380 390 400 410 420 RVMIPNKPDH AFVYRATTNY AGELMETGAK IFIYDNGFIH AKTLVVDGEI ASVGTANMDF 430 440 450 460 470 480 RSFRLNFEVN AFIYEKKMVQ KLEDAFLEDI LKSYQLTPEL YAKRSLWIKF KEAVSRLLSP IL

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIP 11262.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL596173 Genomic DNA. Translation: CAC97873.1.
PIR	AI1762.
RefSeq	NP_471976.1. NC_003212.1.
3D structure databases
ProteinModelPortal	Q927Z0.
ModBase	Search...
Protein-protein interaction databases
STRING	272626.lin2646.
Protocols and materials databases
DNASU	1131482.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC97873; CAC97873; CAC97873.
GeneID	1131482.
KEGG	lin:lin2646.
PATRIC	20302209. VBILisInn102668_2717.
Organism-specific databases
GenoList	LIN2646.
CMR	Search...
Phylogenomic databases
eggNOG	COG1502.
HOGENOM	HOG000077402.
KO	K06131.
OMA	LAINDLE.
ProtClustDB	CLSK564972.
Family and domain databases
HAMAP	MF_00190. Cardiolipin_synth.
InterPro	IPR022924. Cardiolipin_synthase. IPR027379. CLS_N. IPR025202. PLD-like_dom. IPR001736. PLipase_D/transphosphatidylase. IPR015679. PLipase_D_fam. [Graphical view]
PANTHER	PTHR18896. PTHR18896. 1 hit.
Pfam	PF13091. PLDc_2. 2 hits. PF13396. PLDc_N. 1 hit. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
TIGRFAMs	TIGR04265. bac_cardiolipin. 1 hit.
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLS_LISIN

Accession

Primary (citable) accession number: Q927Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program