Q92785 (REQU_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein ubi-d4

Alternative name(s):
Apoptosis response zinc finger protein
BRG1-associated factor 45D
Short name=BAF45D
D4, zinc and double PHD fingers family 2
Protein requiem

Gene names

Name:	DPF2
Synonyms:	BAF45D, REQ, UBID4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Subcellular location	Nucleus. Cytoplasm. Note: 30% nuclear. 70% cytoplasmic.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the requiem/DPF family. Contains 1 C2H2-type zinc finger. Contains 2 PHD-type zinc fingers.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis by extracellular signals Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 391

390

Zinc finger protein ubi-d4

PRO_0000168149

Regions

Zinc finger

209 – 232

24

C2H2-type

Zinc finger

270 – 330

61

PHD-type 1

Zinc finger

327 – 377

51

PHD-type 2

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.13

Modified residue

94

1

Phosphoserine Ref.11

Modified residue

97

1

Phosphoserine Ref.11

Modified residue

142

1

Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14

Modified residue

172

1

Phosphotyrosine Ref.14

Modified residue

176

1

Phosphothreonine Ref.12 Ref.14

Modified residue

200

1

Phosphoserine Ref.12

Modified residue

248

1

Phosphothreonine By similarity

Secondary structure

1

.

.

.

.

.

.

.

391

Helix Strand Turn

Details...

Turn

204 – 206

3

Beta strand

217 – 220

4

Helix

225 – 230

6

Sequences

	Sequence		Length	Mass (Da)
	Q92785 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 1044B4D3036075FC	FASTA	391	44,155
	10 20 30 40 50 60 MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ SNCYIWMEKR 70 80 90 100 110 120 HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP DTDQTLKKEG LISQDGSSLE 130 140 150 160 170 180 ALLRTDPLEK RGAPDPRVDD DSLGEFPVTN SRARKRILEP DDFLDDLDDE DYEEDTPKRR 190 200 210 220 230 240 GKGKSKGKGV GSARKKLDAS ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED 250 260 270 280 290 300 KEDSQPPTPV SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR 310 320 330 340 350 360 SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NICGTSENDD QLLFCDDCDR GYHMYCLTPS 370 380 390 MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcript map for the 2.8-Mb region containing the multiple endocrine neoplasia type 1 locus." Guru S.C., Agarwal S.K., Manickam P., Olufemi S.-E., Crabtree J.S., Weisemann J.M., Kester M.B., Kim Y.S., Wang Y., Emmert-Buck M.R., Liotta L.A., Spiegel A.M., Boguski M.S., Roe B.A., Collins F.S., Burns A.L., Marx S.J., Chandrasekharappa S.C. Genome Res. 7:725-735(1997) [PubMed: 9253601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression and chromosomal localization of the Requiem gene." Gabig T.G., Crean C.D., Klenk A., Long H., Copeland N.G., Gilbert D.J., Jenkins N.A., Quincey D., Parente F., Lespinasse F., Carle G.F., Gaudray P., Zhang C.X., Calender A., Hoeppener J., Kas K., Thakker R.V., Farnebo F. , Teh B.T., Larsson C., Piehl F., Lagercrantz J., Khodaei S., Carson E., Weber G. Mamm. Genome 9:660-665(1998) [PubMed: 9680388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIEHS SNPs program Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[8]	"The d4 gene family in the human genome." Chestkov A.V., Baka I.D., Kost M.V., Georgiev G.P., Buchman V.L. Genomics 36:174-177(1996) [PubMed: 8812431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-64.
[9]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94; SER-97 AND SER-142, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; THR-176 AND SER-200, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[14]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; TYR-172 AND THR-176, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[15]	"Crystal structure of the C2H2-type zinc finger domain of human UBI-D4." Structural genomics consortium (SGC) Submitted (NOV-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 203-249 IN COMPLEX WITH ZINC IONS.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF001433 mRNA. Translation: AAB81203.1.
U94585 mRNA. Translation: AAB58307.1.
BT006718 mRNA. Translation: AAP35364.1.
AY220877 Genomic DNA. Translation: AAO26041.1.
AK291944 mRNA. Translation: BAF84633.1.
CH471076 Genomic DNA. Translation: EAW74375.1.
BC014889 mRNA. Translation: AAH14889.1.
U43920 Genomic DNA. Translation: AAC50687.1.

IPI

IPI00983171.

RefSeq

NP_006259.1. NM_006268.4.

UniGene

Hs.13495.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IUF	X-ray	1.80	A	203-249	[»]

ProteinModelPortal

Q92785.

SMR

Q92785. Positions 203-234, 271-383.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-27575N.

IntAct

Q92785. 5 interactions.

MINT

MINT-1160145.

STRING

Q92785.

PTM databases

PhosphoSite

Q92785.

Polymorphism databases

DMDM

2842711.

Proteomic databases

PeptideAtlas

Q92785.

PRIDE

Q92785.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000252268; ENSP00000252268; ENSG00000133884.

GeneID

5977.

KEGG

hsa:5977.

UCSC

uc001odm.1. human.

Organism-specific databases

CTD

5977.

GeneCards

GC11P065103.

HGNC

HGNC:9964. DPF2.

HPA

HPA020880.

MIM

601671. gene.

neXtProt

NX_Q92785.

PharmGKB

PA34331.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG05917.

GeneTree

ENSGT00530000063194.

HOGENOM

HBG445162.

HOVERGEN

HBG004475.

InParanoid

Q92785.

OMA

DSSHYHH.

OrthoDB

EOG41JZD4.

PhylomeDB

Q92785.

Gene expression databases

ArrayExpress

Q92785.

Bgee

Q92785.

CleanEx

HS_DPF2.

Genevestigator

Q92785.

GermOnline

ENSG00000133884. Homo sapiens.

Family and domain databases

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 1 hit.
G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.

KO

K13196.

Pfam

PF00628. PHD. 1 hit.
[Graphical view]

SMART

SM00249. PHD. 2 hits.
SM00184. RING. 2 hits.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

SUPFAM

SSF57903. FYVE_PHD_ZnF. 2 hits.

PROSITE

PS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

23263.

PMAP-CutDB

Q92785.

SOURCE

Search...

Entry information

Entry name

REQU_HUMAN

Accession

Primary (citable) accession number: Q92785
Secondary accession number(s): A8K7C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.