Reviewed, UniProtKB/Swiss-Prot Q92785 (REQU_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger protein ubi-d4
Alternative name(s):
    Requiem
    Apoptosis response zinc finger protein
    D4, zinc and double PHD fingers family 2

Gene names

Name:	DPF2
Synonyms:	REQ, UBID4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Subcellular location	Nucleus. Cytoplasm. Note: 30% nuclear. 70% cytoplasmic.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the requiem/DPF family. Contains 1 C2H2-type zinc finger. Contains 2 PHD-type zinc fingers.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis by extracellular signals Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Zinc finger protein ubi-d4		PRO_0000168149
Regions
	Zinc finger	209 – 232	24	C2H2-type
	Zinc finger	270 – 330	61	PHD-type 1
	Zinc finger	327 – 377	51	PHD-type 2
Amino acid modifications
	Modified residue	94	1	Phosphoserine Ref.9
	Modified residue	97	1	Phosphoserine Ref.9
	Modified residue	142	1	Phosphoserine Ref.9 Ref.7 Ref.8 Ref.10
	Modified residue	176	1	Phosphothreonine Ref.10
	Modified residue	200	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q92785-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 1044B4D3036075FC	FASTA	391	44,155
	10 20 30 40 50 60 MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ SNCYIWMEKR 70 80 90 100 110 120 HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP DTDQTLKKEG LISQDGSSLE 130 140 150 160 170 180 ALLRTDPLEK RGAPDPRVDD DSLGEFPVTN SRARKRILEP DDFLDDLDDE DYEEDTPKRR 190 200 210 220 230 240 GKGKSKGKGV GSARKKLDAS ILEDRDKPYA CDICGKRYKN RPGLSYHYAH SHLAEEEGED 250 260 270 280 290 300 KEDSQPPTPV SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR 310 320 330 340 350 360 SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NICGTSENDD QLLFCDDCDR GYHMYCLTPS 370 380 390 MSEPPEGSWS CHLCLDLLKE KASIYQNQNS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcript map for the 2.8-Mb region containing the multiple endocrine neoplasia type 1 locus." Guru S.C., Agarwal S.K., Manickam P., Olufemi S.-E., Crabtree J.S., Weisemann J.M., Kester M.B., Kim Y.S., Wang Y., Emmert-Buck M.R., Liotta L.A., Spiegel A.M., Boguski M.S., Roe B.A., Collins F.S., Burns A.L., Marx S.J., Chandrasekharappa S.C. Genome Res. 7:725-735(1997) [PubMed: 9253601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression and chromosomal localization of the Requiem gene." Gabig T.G., Crean C.D., Klenk A., Long H., Copeland N.G., Gilbert D.J., Jenkins N.A., Quincey D., Parente F., Lespinasse F., Carle G.F., Gaudray P., Zhang C.X., Calender A., Hoeppener J., Kas K., Thakker R.V., Farnebo F. , Teh B.T., Larsson C., Piehl F., Lagercrantz J., Khodaei S., Carson E., Weber G. Mamm. Genome 9:660-665(1998) [PubMed: 9680388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NIEHS SNPs program Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[6]	"The d4 gene family in the human genome." Chestkov A.V., Baka I.D., Kost M.V., Georgiev G.P., Buchman V.L. Genomics 36:174-177(1996) [PubMed: 8812431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-64.
[7]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94; SER-97 AND SER-142, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; THR-176 AND SER-200, MASS SPECTROMETRY.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF001433 mRNA. Translation: AAB81203.1. U94585 mRNA. Translation: AAB58307.1. BT006718 mRNA. Translation: AAP35364.1. AY220877 Genomic DNA. Translation: AAO26041.1. BC014889 mRNA. Translation: AAH14889.1. U43920 Genomic DNA. Translation: AAC50687.1.
IPI	IPI00023322.
RefSeq	NP_006259.1.
UniGene	Hs.13495
3D structure databases
HSSP	HSSP built from PDB template 1F62 based on UniProtKB Q9UIG0.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27575N.
IntAct	Q92785. 3 interactions.
PTM databases
PhosphoSite	Q92785.
Proteomic databases
PeptideAtlas	Q92785.
PRIDE	Q92785.
Genome annotation databases
Ensembl	ENSG00000133884. Homo sapiens. [Contig view]
GeneID	5977.
KEGG	hsa:5977.
Organism-specific databases
GeneCards	GC11P064858.
HGNC	HGNC:9964. DPF2.
MIM	601671. gene.
PharmGKB	PA34331.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q92785.
HOVERGEN	Q92785.
OMA	Q92785. ECKCCNL.
Gene expression databases
ArrayExpress	Q92785.
Bgee	Q92785.
CleanEx	HS_DPF2.
GermOnline	ENSG00000133884. Homo sapiens.
Family and domain databases
InterPro	IPR019786. Zinc_finger_PHD-type_CS. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR001841. Znf_RING. [Graphical view]
Pfam	PF00628. PHD. 2 hits. PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00249. PHD. 2 hits. SM00184. RING. 2 hits. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 2 hits. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	23263.
PMAP-CutDB	Q92785.
SOURCE	Search...

Entry information

Entry name

REQU_HUMAN

Accession

Primary (citable) accession number: Q92785

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)