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Q92783 (STAM1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducing adapter molecule 1

Short name=STAM-1
Gene names
Name:STAM
Synonyms:STAM1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length540 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.

Subunit structure

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Probably part of a complex at least composed of HSG, STAM and EPS15. Interacts with STAMBP/AMSH. Interacts with PDGFRB. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.13

Subcellular location

Cytoplasm Probable. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side Probable.

Tissue specificity

Ubiquitously expressed. Ref.1

Domain

The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos. Ref.13

Post-translational modification

Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB. Ref.5 Ref.10

Sequence similarities

Belongs to the STAM family.

Contains 1 ITAM domain.

Contains 1 SH3 domain.

Contains 1 UIM (ubiquitin-interacting motif) repeat.

Contains 1 VHS domain.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q92783-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q92783-2)

The sequence of this isoform differs from the canonical sequence as follows:
     392-403: YYMQSSGVSGSQ → GSGPTIRKPSPS
     404-540: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.5
Chain2 – 540539Signal transducing adapter molecule 1
PRO_0000190145

Regions

Domain16 – 143128VHS
Repeat171 – 19020UIM
Domain210 – 26960SH3
Domain370 – 38718ITAM

Amino acid modifications

Modified residue1561Phosphoserine Ref.11
Modified residue1981Phosphotyrosine Ref.5
Modified residue3811Phosphotyrosine
Modified residue3841Phosphotyrosine

Natural variations

Alternative sequence392 – 40312YYMQS…VSGSQ → GSGPTIRKPSPS in isoform 2.
VSP_014846
Alternative sequence404 – 540137Missing in isoform 2.
VSP_014847
Natural variant2121G → D in a colorectal cancer sample; somatic mutation. Ref.14
VAR_036348

Experimental info

Sequence conflict2671D → Y in AAH30586. Ref.4

Secondary structure

................................... 540
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8D6F07FAE538F36F

FASTA54059,180
        10         20         30         40         50         60 
MPLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI MRRVNHKDPH 

        70         80         90        100        110        120 
VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG HPKVCEKLKA LMVEWTDEFK 

       130        140        150        160        170        180 
NDPQLSLISA MIKNLKEQGV TFPAIGSQAA EQAKASPALV AKDPGTVANK KEEEDLAKAI 

       190        200        210        220        230        240 
ELSLKEQRQQ STTLSTLYPS TSSLLTNHQH EGRKVRAIYD FEAAEDNELT FKAGEIITVL 

       250        260        270        280        290        300 
DDSDPNWWKG ETHQGIGLFP SNFVTADLTA EPEMIKTEKK TVQFSDDVQV ETIEPEPEPA 

       310        320        330        340        350        360 
FIDEDKMDQL LQMLQSTDPS DDQPDLPELL HLEAMCHQMG PLIDEKLEDI DRKHSELSEL 

       370        380        390        400        410        420 
NVKVMEALSL YTKLMNEDPM YSMYAKLQNQ PYYMQSSGVS GSQVYAGPPP SGAYLVAGNA 

       430        440        450        460        470        480 
QMSHLQSYSL PPEQLSSLSQ AVVPPSANPA LPSQQTQAAY PNTMVSSVQG NTYPSQAPVY 

       490        500        510        520        530        540 
SPPPAATAAA ATADVTLYQN AGPNMPQVPN YNLTSSTLPQ PGGSQQPPQP QQPYSQKALL 

« Hide

Isoform 2 [UniParc].

Checksum: 91E858BF4FADC8B5
Show »

FASTA40344,972

References

« Hide 'large scale' references
[1]"Cloning of a novel signal-transducing adaptor molecule containing an SH3 domain and ITAM."
Takeshita T., Arita T., Asao H., Tanaka N., Higuchi M., Kuroda H., Kaneko K., Munakata H., Endo Y., Fujita T., Sugamura K.
Biochem. Biophys. Res. Commun. 225:1035-1039(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-15; 110-119; 137-154; 215-223; 233-247 AND 364-373, TISSUE SPECIFICITY.
Tissue: T-cell.
[2]NHLBI resequencing and genotyping service (RS&G)
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[5]"Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway."
Steen H., Kuster B., Fernandez M., Pandey A., Mann M.
J. Biol. Chem. 277:1031-1039(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 189-213, INTERACTION WITH HGS, CLEAVAGE OF INITIATOR METHIONINE, PHOSPHORYLATION AT TYR-198, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth."
Asao H., Sasaki Y., Arita T., Tanaka N., Endo K., Kasai H., Takeshita T., Endo Y., Fujita T., Sugamura K.
J. Biol. Chem. 272:32785-32791(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HGS.
[7]"Possible involvement of a novel STAM-associated molecule 'AMSH' in intracellular signal transduction mediated by cytokines."
Tanaka N., Kaneko K., Asao H., Kasai H., Endo Y., Fujita T., Takeshita T., Sugamura K.
J. Biol. Chem. 274:19129-19135(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STAMBP.
[8]"STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes."
Bache K.G., Raiborg C., Mehlum A., Stenmark H.
J. Biol. Chem. 278:12513-12521(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HGS, IDENTIFICATION IN A COMPLEX WITH HGS AND EPS15.
[9]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Mutation of tyrosine residue 857 in the PDGF beta-receptor affects cell proliferation but not migration."
Wardega P., Heldin C.H., Lennartsson J.
Cell. Signal. 22:1363-1368(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION, INTERACTION WITH PDGFRB.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo."
Ren X., Hurley J.H.
EMBO J. 29:1045-1054(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 4-143 IN COMPLEX WITH UBIQUITIN, DOMAIN VHS, SUBUNIT.
[14]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-212.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43899 mRNA. Translation: AAC50734.1.
EF445033 Genomic DNA. Translation: ACA06077.1.
EF445033 Genomic DNA. Translation: ACA06078.1.
CH471072 Genomic DNA. Translation: EAW86207.1.
CH471072 Genomic DNA. Translation: EAW86209.1.
BC030586 mRNA. Translation: AAH30586.1.
CCDSCCDS7122.1. [Q92783-1]
PIRJC4916.
RefSeqNP_003464.1. NM_003473.3. [Q92783-1]
UniGeneHs.335391.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L0ANMR-A207-267[»]
3F1IX-ray2.30C/S301-377[»]
3LDZX-ray2.60A/B/C/D4-143[»]
ProteinModelPortalQ92783.
SMRQ92783. Positions 4-143, 211-267, 301-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113722. 55 interactions.
DIPDIP-37761N.
IntActQ92783. 12 interactions.
MINTMINT-734093.
STRING9606.ENSP00000366746.

PTM databases

PhosphoSiteQ92783.

Polymorphism databases

DMDM71153545.

Proteomic databases

MaxQBQ92783.
PaxDbQ92783.
PRIDEQ92783.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000377524; ENSP00000366746; ENSG00000136738. [Q92783-1]
ENST00000565101; ENSP00000456885; ENSG00000260045. [Q92783-1]
GeneID8027.
KEGGhsa:8027.
UCSCuc001ipj.2. human. [Q92783-1]

Organism-specific databases

CTD8027.
GeneCardsGC10P017686.
HGNCHGNC:11357. STAM.
HPACAB034399.
HPA043882.
MIM601899. gene.
neXtProtNX_Q92783.
PharmGKBPA36179.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG309509.
HOGENOMHOG000231952.
HOVERGENHBG053175.
InParanoidQ92783.
KOK04705.
OMAYYMQSPG.
PhylomeDBQ92783.
TreeFamTF315007.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_11123. Membrane Trafficking.
REACT_116125. Disease.
SignaLinkQ92783.

Gene expression databases

ArrayExpressQ92783.
BgeeQ92783.
CleanExHS_STAM.
GenevestigatorQ92783.

Family and domain databases

Gene3D1.25.40.90. 1 hit.
InterProIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. Ubiquitin-int_motif.
IPR002014. VHS.
IPR018205. VHS_subgr.
[Graphical view]
PfamPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00326. SH3. 1 hit.
SM00726. UIM. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ92783.
GeneWikiSignal_transducing_adaptor_molecule.
GenomeRNAi8027.
NextBio30597.
PROQ92783.
SOURCESearch...

Entry information

Entry nameSTAM1_HUMAN
AccessionPrimary (citable) accession number: Q92783
Secondary accession number(s): B0YJ99, D3DRU5, Q8N6D9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM