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Q92783

- STAM1_HUMAN

UniProt

Q92783 - STAM1_HUMAN

Protein

Signal transducing adapter molecule 1

Gene

STAM

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. SH3/SH2 adaptor activity Source: ProtInc

    GO - Biological processi

    1. endosomal transport Source: Reactome
    2. epidermal growth factor receptor signaling pathway Source: Reactome
    3. intracellular protein transport Source: InterPro
    4. membrane organization Source: Reactome
    5. negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
    6. positive regulation of signal transduction Source: GOC
    7. signal transduction Source: ProtInc

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_12484. EGFR downregulation.
    REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    SignaLinkiQ92783.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal transducing adapter molecule 1
    Short name:
    STAM-1
    Gene namesi
    Name:STAM
    Synonyms:STAM1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:11357. STAM.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. early endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36179.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 540539Signal transducing adapter molecule 1PRO_0000190145Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei156 – 1561Phosphoserine2 Publications
    Modified residuei198 – 1981Phosphotyrosine2 Publications
    Modified residuei381 – 3811Phosphotyrosine1 Publication
    Modified residuei384 – 3841Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ92783.
    PaxDbiQ92783.
    PRIDEiQ92783.

    PTM databases

    PhosphoSiteiQ92783.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ92783.
    BgeeiQ92783.
    CleanExiHS_STAM.
    GenevestigatoriQ92783.

    Organism-specific databases

    HPAiCAB034399.
    HPA043882.

    Interactioni

    Subunit structurei

    Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Probably part of a complex at least composed of HSG, STAM and EPS15. Interacts with STAMBP/AMSH. Interacts with PDGFRB.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LCP2Q130943EBI-752333,EBI-346946
    STAMBPO956306EBI-752333,EBI-396676
    UBBP0CG539EBI-752333,EBI-5333021From a different organism.
    UBCP0CG482EBI-752333,EBI-3390054

    Protein-protein interaction databases

    BioGridi113722. 56 interactions.
    DIPiDIP-37761N.
    IntActiQ92783. 12 interactions.
    MINTiMINT-734093.
    STRINGi9606.ENSP00000366746.

    Structurei

    Secondary structure

    1
    540
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 167
    Beta strandi21 – 233
    Helixi26 – 3611
    Helixi42 – 5413
    Helixi59 – 7517
    Helixi78 – 847
    Helixi87 – 9913
    Helixi102 – 11918
    Helixi123 – 1253
    Helixi127 – 13711
    Beta strandi213 – 2197
    Beta strandi236 – 2449
    Beta strandi247 – 2515
    Beta strandi256 – 2583
    Beta strandi264 – 2663
    Helixi304 – 31512
    Helixi327 – 37549

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L0ANMR-A207-267[»]
    3F1IX-ray2.30C/S301-377[»]
    3LDZX-ray2.60A/B/C/D4-143[»]
    ProteinModelPortaliQ92783.
    SMRiQ92783. Positions 4-143, 211-267, 301-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ92783.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 143128VHSPROSITE-ProRule annotationAdd
    BLAST
    Repeati171 – 19020UIMAdd
    BLAST
    Domaini210 – 26960SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini370 – 38718ITAMAdd
    BLAST

    Domaini

    The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.1 Publication

    Sequence similaritiesi

    Belongs to the STAM family.Curated
    Contains 1 ITAM domain.Curated
    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 1 UIM (ubiquitin-interacting motif) repeat.PROSITE-ProRule annotation
    Contains 1 VHS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG309509.
    HOGENOMiHOG000231952.
    HOVERGENiHBG053175.
    InParanoidiQ92783.
    KOiK04705.
    OMAiYYMQSPG.
    PhylomeDBiQ92783.
    TreeFamiTF315007.

    Family and domain databases

    Gene3Di1.25.40.90. 1 hit.
    InterProiIPR008942. ENTH_VHS.
    IPR001452. SH3_domain.
    IPR003903. Ubiquitin-int_motif.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view]
    PfamiPF00018. SH3_1. 1 hit.
    PF02809. UIM. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00326. SH3. 1 hit.
    SM00726. UIM. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    PS50330. UIM. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q92783-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI    50
    MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG 100
    HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA 150
    EQAKASPALV AKDPGTVANK KEEEDLAKAI ELSLKEQRQQ STTLSTLYPS 200
    TSSLLTNHQH EGRKVRAIYD FEAAEDNELT FKAGEIITVL DDSDPNWWKG 250
    ETHQGIGLFP SNFVTADLTA EPEMIKTEKK TVQFSDDVQV ETIEPEPEPA 300
    FIDEDKMDQL LQMLQSTDPS DDQPDLPELL HLEAMCHQMG PLIDEKLEDI 350
    DRKHSELSEL NVKVMEALSL YTKLMNEDPM YSMYAKLQNQ PYYMQSSGVS 400
    GSQVYAGPPP SGAYLVAGNA QMSHLQSYSL PPEQLSSLSQ AVVPPSANPA 450
    LPSQQTQAAY PNTMVSSVQG NTYPSQAPVY SPPPAATAAA ATADVTLYQN 500
    AGPNMPQVPN YNLTSSTLPQ PGGSQQPPQP QQPYSQKALL 540
    Length:540
    Mass (Da):59,180
    Last modified:January 23, 2007 - v3
    Checksum:i8D6F07FAE538F36F
    GO
    Isoform 2 (identifier: Q92783-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         392-403: YYMQSSGVSGSQ → GSGPTIRKPSPS
         404-540: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:403
    Mass (Da):44,972
    Checksum:i91E858BF4FADC8B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti267 – 2671D → Y in AAH30586. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti212 – 2121G → D in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_036348

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei392 – 40312YYMQS…VSGSQ → GSGPTIRKPSPS in isoform 2. 1 PublicationVSP_014846Add
    BLAST
    Alternative sequencei404 – 540137Missing in isoform 2. 1 PublicationVSP_014847Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43899 mRNA. Translation: AAC50734.1.
    EF445033 Genomic DNA. Translation: ACA06077.1.
    EF445033 Genomic DNA. Translation: ACA06078.1.
    CH471072 Genomic DNA. Translation: EAW86207.1.
    CH471072 Genomic DNA. Translation: EAW86209.1.
    BC030586 mRNA. Translation: AAH30586.1.
    CCDSiCCDS7122.1. [Q92783-1]
    PIRiJC4916.
    RefSeqiNP_003464.1. NM_003473.3. [Q92783-1]
    UniGeneiHs.335391.

    Genome annotation databases

    EnsembliENST00000377524; ENSP00000366746; ENSG00000136738. [Q92783-1]
    GeneIDi8027.
    KEGGihsa:8027.
    UCSCiuc001ipj.2. human. [Q92783-1]

    Polymorphism databases

    DMDMi71153545.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43899 mRNA. Translation: AAC50734.1 .
    EF445033 Genomic DNA. Translation: ACA06077.1 .
    EF445033 Genomic DNA. Translation: ACA06078.1 .
    CH471072 Genomic DNA. Translation: EAW86207.1 .
    CH471072 Genomic DNA. Translation: EAW86209.1 .
    BC030586 mRNA. Translation: AAH30586.1 .
    CCDSi CCDS7122.1. [Q92783-1 ]
    PIRi JC4916.
    RefSeqi NP_003464.1. NM_003473.3. [Q92783-1 ]
    UniGenei Hs.335391.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L0A NMR - A 207-267 [» ]
    3F1I X-ray 2.30 C/S 301-377 [» ]
    3LDZ X-ray 2.60 A/B/C/D 4-143 [» ]
    ProteinModelPortali Q92783.
    SMRi Q92783. Positions 4-143, 211-267, 301-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113722. 56 interactions.
    DIPi DIP-37761N.
    IntActi Q92783. 12 interactions.
    MINTi MINT-734093.
    STRINGi 9606.ENSP00000366746.

    PTM databases

    PhosphoSitei Q92783.

    Polymorphism databases

    DMDMi 71153545.

    Proteomic databases

    MaxQBi Q92783.
    PaxDbi Q92783.
    PRIDEi Q92783.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000377524 ; ENSP00000366746 ; ENSG00000136738 . [Q92783-1 ]
    GeneIDi 8027.
    KEGGi hsa:8027.
    UCSCi uc001ipj.2. human. [Q92783-1 ]

    Organism-specific databases

    CTDi 8027.
    GeneCardsi GC10P017686.
    HGNCi HGNC:11357. STAM.
    HPAi CAB034399.
    HPA043882.
    MIMi 601899. gene.
    neXtProti NX_Q92783.
    PharmGKBi PA36179.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG309509.
    HOGENOMi HOG000231952.
    HOVERGENi HBG053175.
    InParanoidi Q92783.
    KOi K04705.
    OMAi YYMQSPG.
    PhylomeDBi Q92783.
    TreeFami TF315007.

    Enzyme and pathway databases

    Reactomei REACT_12484. EGFR downregulation.
    REACT_27258. Endosomal Sorting Complex Required For Transport (ESCRT).
    SignaLinki Q92783.

    Miscellaneous databases

    EvolutionaryTracei Q92783.
    GeneWikii Signal_transducing_adaptor_molecule.
    GenomeRNAii 8027.
    NextBioi 30597.
    PROi Q92783.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q92783.
    Bgeei Q92783.
    CleanExi HS_STAM.
    Genevestigatori Q92783.

    Family and domain databases

    Gene3Di 1.25.40.90. 1 hit.
    InterProi IPR008942. ENTH_VHS.
    IPR001452. SH3_domain.
    IPR003903. Ubiquitin-int_motif.
    IPR002014. VHS.
    IPR018205. VHS_subgr.
    [Graphical view ]
    Pfami PF00018. SH3_1. 1 hit.
    PF02809. UIM. 1 hit.
    PF00790. VHS. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00326. SH3. 1 hit.
    SM00726. UIM. 1 hit.
    SM00288. VHS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    PS50330. UIM. 1 hit.
    PS50179. VHS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a novel signal-transducing adaptor molecule containing an SH3 domain and ITAM."
      Takeshita T., Arita T., Asao H., Tanaka N., Higuchi M., Kuroda H., Kaneko K., Munakata H., Endo Y., Fujita T., Sugamura K.
      Biochem. Biophys. Res. Commun. 225:1035-1039(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 2-15; 110-119; 137-154; 215-223; 233-247 AND 364-373, TISSUE SPECIFICITY.
      Tissue: T-cell.
    2. NHLBI resequencing and genotyping service (RS&G)
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    5. "Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway."
      Steen H., Kuster B., Fernandez M., Pandey A., Mann M.
      J. Biol. Chem. 277:1031-1039(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 189-213, INTERACTION WITH HGS, CLEAVAGE OF INITIATOR METHIONINE, PHOSPHORYLATION AT TYR-198, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth."
      Asao H., Sasaki Y., Arita T., Tanaka N., Endo K., Kasai H., Takeshita T., Endo Y., Fujita T., Sugamura K.
      J. Biol. Chem. 272:32785-32791(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HGS.
    7. "Possible involvement of a novel STAM-associated molecule 'AMSH' in intracellular signal transduction mediated by cytokines."
      Tanaka N., Kaneko K., Asao H., Kasai H., Endo Y., Fujita T., Takeshita T., Sugamura K.
      J. Biol. Chem. 274:19129-19135(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STAMBP.
    8. "STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes."
      Bache K.G., Raiborg C., Mehlum A., Stenmark H.
      J. Biol. Chem. 278:12513-12521(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HGS, IDENTIFICATION IN A COMPLEX WITH HGS AND EPS15.
    9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Mutation of tyrosine residue 857 in the PDGF beta-receptor affects cell proliferation but not migration."
      Wardega P., Heldin C.H., Lennartsson J.
      Cell. Signal. 22:1363-1368(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, INTERACTION WITH PDGFRB.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo."
      Ren X., Hurley J.H.
      EMBO J. 29:1045-1054(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 4-143 IN COMPLEX WITH UBIQUITIN, DOMAIN VHS, SUBUNIT.
    14. Cited for: VARIANT [LARGE SCALE ANALYSIS] ASP-212.

    Entry informationi

    Entry nameiSTAM1_HUMAN
    AccessioniPrimary (citable) accession number: Q92783
    Secondary accession number(s): B0YJ99, D3DRU5, Q8N6D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3