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Protein

Signal transducing adapter molecule 1

Gene

STAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

  • autophagy Source: ParkinsonsUK-UCL
  • endosomal transport Source: Reactome
  • intracellular protein transport Source: InterPro
  • multivesicular body assembly Source: ParkinsonsUK-UCL
  • negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
  • positive regulation of exosomal secretion Source: UniProtKB
  • regulation of extracellular exosome assembly Source: UniProtKB
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136738-MONOMER.
ReactomeiR-HSA-182971. EGFR downregulation.
R-HSA-5689901. Metalloprotease DUBs.
R-HSA-6807004. Negative regulation of MET activity.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SignaLinkiQ92783.
SIGNORiQ92783.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 1
Short name:
STAM-1
Gene namesi
Name:STAM
Synonyms:STAM1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:11357. STAM.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • early endosome membrane Source: UniProtKB-SubCell
  • ESCRT-0 complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8027.
OpenTargetsiENSG00000136738.
PharmGKBiPA36179.

Polymorphism and mutation databases

BioMutaiSTAM.
DMDMi71153545.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001901452 – 540Signal transducing adapter molecule 1Add BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineCombined sources1
Modified residuei198Phosphotyrosine1 Publication1
Modified residuei381Phosphotyrosine1 Publication1
Modified residuei384Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ92783.
MaxQBiQ92783.
PaxDbiQ92783.
PeptideAtlasiQ92783.
PRIDEiQ92783.

PTM databases

iPTMnetiQ92783.
PhosphoSitePlusiQ92783.
SwissPalmiQ92783.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000136738.
CleanExiHS_STAM.
ExpressionAtlasiQ92783. baseline and differential.
GenevisibleiQ92783. HS.

Organism-specific databases

HPAiCAB034399.
HPA043882.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS (PubMed:19278655). Probably part of a complex at least composed of HSG, STAM and EPS15. Interacts with STAMBP/AMSH. Interacts with PDGFRB.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LCP2Q130943EBI-752333,EBI-346946
STAMBPO956307EBI-752333,EBI-396676
UBBP0CG539EBI-752333,EBI-5333021From a different organism.
UBCP0CG482EBI-752333,EBI-3390054

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi113722. 65 interactors.
DIPiDIP-37761N.
IntActiQ92783. 16 interactors.
MINTiMINT-734093.
STRINGi9606.ENSP00000366746.

Structurei

Secondary structure

1540
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 16Combined sources7
Beta strandi21 – 23Combined sources3
Helixi26 – 36Combined sources11
Helixi42 – 54Combined sources13
Helixi59 – 75Combined sources17
Helixi78 – 84Combined sources7
Helixi87 – 99Combined sources13
Helixi102 – 119Combined sources18
Helixi123 – 125Combined sources3
Helixi127 – 137Combined sources11
Beta strandi213 – 219Combined sources7
Beta strandi236 – 244Combined sources9
Beta strandi247 – 251Combined sources5
Beta strandi256 – 258Combined sources3
Beta strandi264 – 266Combined sources3
Helixi304 – 315Combined sources12
Helixi327 – 375Combined sources49

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L0ANMR-A207-267[»]
3F1IX-ray2.30C/S301-377[»]
3LDZX-ray2.60A/B/C/D4-143[»]
ProteinModelPortaliQ92783.
SMRiQ92783.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92783.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 143VHSPROSITE-ProRule annotationAdd BLAST128
Domaini171 – 190UIMPROSITE-ProRule annotationAdd BLAST20
Domaini210 – 269SH3PROSITE-ProRule annotationAdd BLAST60
Domaini370 – 387ITAMAdd BLAST18

Domaini

The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.1 Publication

Sequence similaritiesi

Belongs to the STAM family.Curated
Contains 1 ITAM domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00860000133797.
HOGENOMiHOG000231952.
HOVERGENiHBG053175.
InParanoidiQ92783.
KOiK04705.
OMAiPDVTIYQ.
OrthoDBiEOG091G0H1Y.
PhylomeDBiQ92783.
TreeFamiTF315007.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92783-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLFATNPFD QDVEKATSEM NTAEDWGLIL DICDKVGQSR TGPKDCLRSI
60 70 80 90 100
MRRVNHKDPH VAMQALTLLG ACVSNCGKIF HLEVCSRDFA SEVSNVLNKG
110 120 130 140 150
HPKVCEKLKA LMVEWTDEFK NDPQLSLISA MIKNLKEQGV TFPAIGSQAA
160 170 180 190 200
EQAKASPALV AKDPGTVANK KEEEDLAKAI ELSLKEQRQQ STTLSTLYPS
210 220 230 240 250
TSSLLTNHQH EGRKVRAIYD FEAAEDNELT FKAGEIITVL DDSDPNWWKG
260 270 280 290 300
ETHQGIGLFP SNFVTADLTA EPEMIKTEKK TVQFSDDVQV ETIEPEPEPA
310 320 330 340 350
FIDEDKMDQL LQMLQSTDPS DDQPDLPELL HLEAMCHQMG PLIDEKLEDI
360 370 380 390 400
DRKHSELSEL NVKVMEALSL YTKLMNEDPM YSMYAKLQNQ PYYMQSSGVS
410 420 430 440 450
GSQVYAGPPP SGAYLVAGNA QMSHLQSYSL PPEQLSSLSQ AVVPPSANPA
460 470 480 490 500
LPSQQTQAAY PNTMVSSVQG NTYPSQAPVY SPPPAATAAA ATADVTLYQN
510 520 530 540
AGPNMPQVPN YNLTSSTLPQ PGGSQQPPQP QQPYSQKALL
Length:540
Mass (Da):59,180
Last modified:January 23, 2007 - v3
Checksum:i8D6F07FAE538F36F
GO
Isoform 2 (identifier: Q92783-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-403: YYMQSSGVSGSQ → GSGPTIRKPSPS
     404-540: Missing.

Note: No experimental confirmation available.
Show »
Length:403
Mass (Da):44,972
Checksum:i91E858BF4FADC8B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti267D → Y in AAH30586 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036348212G → D in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014846392 – 403YYMQS…VSGSQ → GSGPTIRKPSPS in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_014847404 – 540Missing in isoform 2. 1 PublicationAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43899 mRNA. Translation: AAC50734.1.
EF445033 Genomic DNA. Translation: ACA06077.1.
EF445033 Genomic DNA. Translation: ACA06078.1.
CH471072 Genomic DNA. Translation: EAW86207.1.
CH471072 Genomic DNA. Translation: EAW86209.1.
BC030586 mRNA. Translation: AAH30586.1.
CCDSiCCDS7122.1. [Q92783-1]
PIRiJC4916.
RefSeqiNP_003464.1. NM_003473.3. [Q92783-1]
UniGeneiHs.335391.

Genome annotation databases

EnsembliENST00000377524; ENSP00000366746; ENSG00000136738. [Q92783-1]
GeneIDi8027.
KEGGihsa:8027.
UCSCiuc001ipj.3. human. [Q92783-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43899 mRNA. Translation: AAC50734.1.
EF445033 Genomic DNA. Translation: ACA06077.1.
EF445033 Genomic DNA. Translation: ACA06078.1.
CH471072 Genomic DNA. Translation: EAW86207.1.
CH471072 Genomic DNA. Translation: EAW86209.1.
BC030586 mRNA. Translation: AAH30586.1.
CCDSiCCDS7122.1. [Q92783-1]
PIRiJC4916.
RefSeqiNP_003464.1. NM_003473.3. [Q92783-1]
UniGeneiHs.335391.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L0ANMR-A207-267[»]
3F1IX-ray2.30C/S301-377[»]
3LDZX-ray2.60A/B/C/D4-143[»]
ProteinModelPortaliQ92783.
SMRiQ92783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113722. 65 interactors.
DIPiDIP-37761N.
IntActiQ92783. 16 interactors.
MINTiMINT-734093.
STRINGi9606.ENSP00000366746.

PTM databases

iPTMnetiQ92783.
PhosphoSitePlusiQ92783.
SwissPalmiQ92783.

Polymorphism and mutation databases

BioMutaiSTAM.
DMDMi71153545.

Proteomic databases

EPDiQ92783.
MaxQBiQ92783.
PaxDbiQ92783.
PeptideAtlasiQ92783.
PRIDEiQ92783.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377524; ENSP00000366746; ENSG00000136738. [Q92783-1]
GeneIDi8027.
KEGGihsa:8027.
UCSCiuc001ipj.3. human. [Q92783-1]

Organism-specific databases

CTDi8027.
DisGeNETi8027.
GeneCardsiSTAM.
HGNCiHGNC:11357. STAM.
HPAiCAB034399.
HPA043882.
MIMi601899. gene.
neXtProtiNX_Q92783.
OpenTargetsiENSG00000136738.
PharmGKBiPA36179.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00860000133797.
HOGENOMiHOG000231952.
HOVERGENiHBG053175.
InParanoidiQ92783.
KOiK04705.
OMAiPDVTIYQ.
OrthoDBiEOG091G0H1Y.
PhylomeDBiQ92783.
TreeFamiTF315007.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136738-MONOMER.
ReactomeiR-HSA-182971. EGFR downregulation.
R-HSA-5689901. Metalloprotease DUBs.
R-HSA-6807004. Negative regulation of MET activity.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.
R-HSA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SignaLinkiQ92783.
SIGNORiQ92783.

Miscellaneous databases

EvolutionaryTraceiQ92783.
GeneWikiiSignal_transducing_adaptor_molecule.
GenomeRNAii8027.
PROiQ92783.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136738.
CleanExiHS_STAM.
ExpressionAtlasiQ92783. baseline and differential.
GenevisibleiQ92783. HS.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF02809. UIM. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTAM1_HUMAN
AccessioniPrimary (citable) accession number: Q92783
Secondary accession number(s): B0YJ99, D3DRU5, Q8N6D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.