Q92782 (DPF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein neuro-d4

Alternative name(s):
BRG1-associated factor 45B
Short name=BAF45B
D4, zinc and double PHD fingers family 1

Gene names

Name:	DPF1
Synonyms:	BAF45B, NEUD4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth By similarity.
Subunit structure	Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin By similarity.
Subcellular location	Cytoplasm Potential. Nucleus Potential.
Sequence similarities	Belongs to the requiem/DPF family. Contains 2 PHD-type zinc fingers.
Caution	It is uncertain whether Met-1 or Met-28 is the initiator.
Sequence caution	The sequence AAC50685.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAI25154.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence EAW56763.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Neurogenesis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	induction of apoptosis Traceable author statement Ref.5. Source: ProtInc nervous system development Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nBAF complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q92782-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92782-2)

The sequence of this isoform differs from the canonical sequence as follows:
     226-226: K → ICGKRYKNRPGLSYHYTHTHLAEEEGEENAERHALPFHRKNNHKQ
     321-330: Missing.

Isoform 3 (identifier: Q92782-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.
     226-226: K → ICGKRYKNRPGLSYHYTHTHLAEEEGEENAERHALPFHRKNNHKQ
     321-330: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Zinc finger protein neuro-d4		PRO_0000168145
Regions
	Zinc finger	254 – 311	58	PHD-type 1
	Zinc finger	308 – 368	61	PHD-type 2
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform 3.		VSP_040224
	Alternative sequence	226	1	K → ICGKRYKNRPGLSYHYTHTH LAEEEGEENAERHALPFHRK NNHKQ in isoform 2 and isoform 3.		VSP_040225
	Alternative sequence	321 – 330	10	Missing in isoform 2 and isoform 3.		VSP_040226
Experimental info
	Sequence conflict	25	1	G → T in AAI25154. Ref.4
	Sequence conflict	34	1	G → S in AAC50685. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: C3550A83807B4FDB	FASTA	380	42,502
	10 20 30 40 50 60 MGGLSARPTA GRTDPAGTCW GQDPGSKMAT VIPGPLSLGE DFYREAIEHC RSYNARLCAE 70 80 90 100 110 120 RSLRLPFLDS QTGVAQNNCY IWMEKTHRGP GLAPGQIYTY PARCWRKKRR LNILEDPRLR 130 140 150 160 170 180 PCEYKIDCEA PLKKEGGLPE GPVLEALLCA ETGEKKIELK EEETIMDCQK QQLLEFPHDL 190 200 210 220 230 240 EVEDLEDDIP RRKNRAKGKA YGIGGLRKRQ DTASLEDRDK PYVCDKFYKE LAWVPEAQRK 250 260 270 280 290 300 HTAKKAPDGT VIPNGYCDFC LGGSKKTGCP EDLISCADCG RSGHPSCLQF TVNMTAAVRT 310 320 330 340 350 360 YRWQCIECKS CSLCGTSEND GASWAGLTPQ DQLLFCDDCD RGYHMYCLSP PMAEPPEGSW 370 380 SCHLCLRHLK EKASAYITLT
	Isoform 2 [UniParc]. Checksum: 343C21142565D5FF	FASTA	414	46,785
	10 20 30 40 50 60 MGGLSARPTA GRTDPAGTCW GQDPGSKMAT VIPGPLSLGE DFYREAIEHC RSYNARLCAE 70 80 90 100 110 120 RSLRLPFLDS QTGVAQNNCY IWMEKTHRGP GLAPGQIYTY PARCWRKKRR LNILEDPRLR 130 140 150 160 170 180 PCEYKIDCEA PLKKEGGLPE GPVLEALLCA ETGEKKIELK EEETIMDCQK QQLLEFPHDL 190 200 210 220 230 240 EVEDLEDDIP RRKNRAKGKA YGIGGLRKRQ DTASLEDRDK PYVCDICGKR YKNRPGLSYH 250 260 270 280 290 300 YTHTHLAEEE GEENAERHAL PFHRKNNHKQ FYKELAWVPE AQRKHTAKKA PDGTVIPNGY 310 320 330 340 350 360 CDFCLGGSKK TGCPEDLISC ADCGRSGHPS CLQFTVNMTA AVRTYRWQCI ECKSCSLCGT 370 380 390 400 410 SENDDQLLFC DDCDRGYHMY CLSPPMAEPP EGSWSCHLCL RHLKEKASAY ITLT
	Isoform 3 [UniParc]. Checksum: A3E86C6D5C87F240	FASTA	332	37,869
	10 20 30 40 50 60 MEKTHRGPGL APGQIYTYPA RCWRKKRRLN ILEDPRLRPC EYKIDCEAPL KKEGGLPEGP 70 80 90 100 110 120 VLEALLCAET GEKKIELKEE ETIMDCQKQQ LLEFPHDLEV EDLEDDIPRR KNRAKGKAYG 130 140 150 160 170 180 IGGLRKRQDT ASLEDRDKPY VCDICGKRYK NRPGLSYHYT HTHLAEEEGE ENAERHALPF 190 200 210 220 230 240 HRKNNHKQFY KELAWVPEAQ RKHTAKKAPD GTVIPNGYCD FCLGGSKKTG CPEDLISCAD 250 260 270 280 290 300 CGRSGHPSCL QFTVNMTAAV RTYRWQCIEC KSCSLCGTSE NDDQLLFCDD CDRGYHMYCL 310 320 330 SPPMAEPPEG SWSCHLCLRH LKEKASAYIT LT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-184 (ISOFORM 1). Tissue: Amygdala and Cerebellum.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-380 (ISOFORM 2).
[5]	"The d4 gene family in the human genome." Chestkov A.V., Baka I.D., Kost M.V., Georgiev G.P., Buchman V.L. Genomics 36:174-177(1996) [PubMed: 8812431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-380 (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK094632 mRNA. Translation: BAG52900.1. DA076421 mRNA. No translation available. AC011479 Genomic DNA. No translation available. CH471126 Genomic DNA. Translation: EAW56763.1. Sequence problems. BC125153 mRNA. Translation: AAI25154.1. Different initiation. U43843 mRNA. Translation: AAC50685.1. Different initiation.
IPI	IPI00216452. IPI00291761. IPI00642063.
RefSeq	NP_001128627.1. NM_001135155.1. NP_001128628.1. NM_001135156.1. NP_004638.2. NM_004647.2.
UniGene	Hs.631576.
3D structure databases
ProteinModelPortal	Q92782.
SMR	Q92782. Positions 256-374.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92782. 1 interaction.
MINT	MINT-4302537.
STRING	Q92782.
PTM databases
PhosphoSite	Q92782.
Polymorphism databases
DMDM	2500145.
Proteomic databases
PRIDE	Q92782.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355526; ENSP00000347716; ENSG00000011332. ENST00000412732; ENSP00000412098; ENSG00000011332. ENST00000414789; ENSP00000391884; ENSG00000011332. ENST00000420980; ENSP00000397354; ENSG00000011332. ENST00000434076; ENSP00000413691; ENSG00000011332. ENST00000437720; ENSP00000394981; ENSG00000011332. ENST00000444314; ENSP00000400336; ENSG00000011332. ENST00000456296; ENSP00000411569; ENSG00000011332.
GeneID	8193.
KEGG	hsa:8193.
UCSC	uc002ohl.3. human. uc002ohm.3. human. uc002ohn.3. human.
Organism-specific databases
CTD	8193.
GeneCards	GC19M038701.
HGNC	HGNC:20225. DPF1.
MIM	601670. gene.
neXtProt	NX_Q92782.
PharmGKB	PA134879894.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271547.
GeneTree	ENSGT00530000063194.
HOGENOM	HOG000217918.
HOVERGEN	HBG004475.
InParanoid	Q92782.
OMA	CEYKIDC.
OrthoDB	EOG4T4CX1.
Gene expression databases
ArrayExpress	Q08AJ0.
Bgee	Q92782.
CleanEx	HS_DPF1.
Genevestigator	Q92782.
GermOnline	ENSG00000011332. Homo sapiens.
Family and domain databases
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 2 hits.
InterPro	IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF00628. PHD. 1 hit. [Graphical view]
SMART	SM00249. PHD. 2 hits. SM00184. RING. 2 hits. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	30890.
SOURCE	Search...

Entry information

Entry name

DPF1_HUMAN

Accession

Primary (citable) accession number: Q92782
Secondary accession number(s): B3KSY8, Q08AJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	May 16, 2012
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.