Q92772 (CDKL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase-like 2
EC=2.7.11.22

Alternative name(s):
Protein kinase p56 KKIAMRE
Serine/threonine-protein kinase KKIAMRE

Gene names

Name:

CDKL2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Expressed in testis and kidney, and at lower level in brain and lung. Ref.1
Domain	The [NKR]KIAxRE motif seems to be a cyclin-binding region.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sex differentiation Traceable author statement Ref.1. Source: ProtInc signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	centrosome Inferred from direct assay. Source: HPA cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC protein kinase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 493

493

Cyclin-dependent kinase-like 2

PRO_0000085814

Regions

Domain

4 – 287

284

Protein kinase

Nucleotide binding

10 – 18

9

ATP By similarity

Motif

45 – 51

7

[NKR]KIAxRE

Sites

Active site

126

1

Proton acceptor By similarity

Binding site

33

1

ATP By similarity

Natural variations

Natural variant

77

1

Y → S.
Corresponds to variant rs35921414 [ dbSNP | Ensembl ].

VAR_053928

Natural variant

98

1

L → I in an ovarian papillary serous adenocarcinoma sample; somatic mutation. Ref.5

VAR_041987

Natural variant

132

1

I → T.
Corresponds to variant rs17000707 [ dbSNP | Ensembl ].

VAR_053929

Natural variant

149

1

R → Q in an ovarian mucinous carcinoma sample; somatic mutation. Ref.5

VAR_041988

Natural variant

197

1

M → T. Ref.5
Corresponds to variant rs56343717 [ dbSNP | Ensembl ].

VAR_041989

Natural variant

411

1

A → V. Ref.5
Corresponds to variant rs56231363 [ dbSNP | Ensembl ].

VAR_041990

Secondary structure

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493

Helix Strand Turn

Details...

Helix

1 – 3

3

Beta strand

4 – 9

6

Helix

10 – 13

4

Beta strand

16 – 23

8

Turn

24 – 26

3

Beta strand

29 – 37

9

Helix

42 – 57

16

Beta strand

66 – 72

7

Beta strand

75 – 81

7

Beta strand

84 – 86

3

Helix

87 – 93

7

Helix

100 – 119

20

Helix

129 – 131

3

Beta strand

132 – 134

3

Beta strand

140 – 142

3

Helix

147 – 150

4

Helix

154 – 163

10

Helix

165 – 167

3

Helix

170 – 173

4

Helix

182 – 197

16

Helix

207 – 218

12

Helix

223 – 231

9

Helix

233 – 235

3

Helix

249 – 252

4

Helix

258 – 267

10

Helix

272 – 274

3

Helix

278 – 283

6

Helix

285 – 288

4

Helix

289 – 291

3

Helix

292 – 301

10

Sequences

	Sequence		Length	Mass (Da)
	Q92772 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 5CC20A91CBF89EFE	FASTA	493	56,019
	10 20 30 40 50 60 MEKYENLGLV GEGSYGMVMK CRNKDTGRIV AIKKFLESDD DKMVKKIAMR EIKLLKQLRH 70 80 90 100 110 120 ENLVNLLEVC KKKKRWYLVF EFVDHTILDD LELFPNGLDY QVVQKYLFQI INGIGFCHSH 130 140 150 160 170 180 NIIHRDIKPE NILVSQSGVV KLCDFGFART LAAPGEVYTD YVATRWYRAP ELLVGDVKYG 190 200 210 220 230 240 KAVDVWAIGC LVTEMFMGEP LFPGDSDIDQ LYHIMMCLGN LIPRHQELFN KNPVFAGVRL 250 260 270 280 290 300 PEIKEREPLE RRYPKLSEVV IDLAKKCLHI DPDKRPFCAE LLHHDFFQMD GFAERFSQEL 310 320 330 340 350 360 QLKVQKDARN VSLSKKSQNR KKEKEKDDSL VEERKTLVVQ DTNADPKIKD YKLFKIKGSK 370 380 390 400 410 420 IDGEKAEKGN RASNASCLHD SRTSHNKIVP STSLKDCSNV SVDHTRNPSV AIPPLTHNLS 430 440 450 460 470 480 AVAPSINSGM GTETIPIQGY RVDEKTKKCS IPFVKPNRHS PSGIYNINVT TLVSGPPLSD 490 DSGADLPQME HQH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE." Taglienti C.A., Wysk M., Davis R.J. Oncogene 13:2563-2574(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Fetal brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ILE-98; GLN-149; THR-197 AND VAL-411.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U35146 mRNA. Translation: AAC50918.1.
AK312490 mRNA. Translation: BAG35392.1.
CH471057 Genomic DNA. Translation: EAX05740.1.
BC093646 mRNA. Translation: AAH93646.1.
BC093981 mRNA. Translation: AAH93981.1.

IPI

IPI00940400.

RefSeq

NP_003939.1. NM_003948.3.

UniGene

Hs.591698.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4AAA	X-ray	1.53	A	1-308	[»]
4BBM	X-ray	2.00	A/B	1-308	[»]

ProteinModelPortal

Q92772.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000306340.

PTM databases

PhosphoSite

Q92772.

Polymorphism databases

DMDM

74762639.

Proteomic databases

PaxDb

Q92772.

PRIDE

Q92772.

Protocols and materials databases

DNASU

8999.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000429927; ENSP00000412365; ENSG00000138769.

GeneID

8999.

KEGG

hsa:8999.

UCSC

uc003hiq.3. human.

Organism-specific databases

CTD

8999.

GeneCards

GC04M076501.

HGNC

HGNC:1782. CDKL2.

HPA

HPA040672.

MIM

603442. gene.

neXtProt

NX_Q92772.

PharmGKB

PA26318.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0515.

HOVERGEN

HBG080204.

InParanoid

Q92772.

KO

K08824.

OrthoDB

EOG4X0MS0.

PhylomeDB

Q92772.

Enzyme and pathway databases

SignaLink

Q92772.

Gene expression databases

ArrayExpress

Q92772.

Bgee

Q92772.

CleanEx

HS_CDKL2.

Genevestigator

Q92772.

GermOnline

ENSG00000138769. Homo sapiens.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q92772.

ChEMBL

CHEMBL5728.

ChiTaRS

CDKL2. human.

GenomeRNAi

8999.

NextBio

33747.

SOURCE

Search...

Entry information

Entry name

CDKL2_HUMAN

Accession

Primary (citable) accession number: Q92772
Secondary accession number(s): B2R695

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.