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Protein

Transcription initiation factor TFIID subunit 4B

Gene

TAF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.1 PublicationBy similarity3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • NF-kappaB binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4B
Alternative name(s):
Transcription initiation factor TFIID 105 kDa subunit
Short name:
TAF(II)105
Short name:
TAFII-105
Short name:
TAFII105
Gene namesi
Name:TAF4B
Synonyms:TAF2C2, TAFII105
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:11538. TAF4B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Spermatogenic failure 13 (SPGF13)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.
See also OMIM:615841

Organism-specific databases

MalaCardsiTAF4B.
MIMi615841. phenotype.
Orphaneti399805. Male infertility with azoospermia or oligozoospermia due to single gene mutation.
PharmGKBiPA36313.

Polymorphism and mutation databases

BioMutaiTAF4B.
DMDMi119370533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Transcription initiation factor TFIID subunit 4BPRO_0000118872Add
BLAST

Post-translational modificationi

Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ92750.
PaxDbiQ92750.
PRIDEiQ92750.

PTM databases

iPTMnetiQ92750.
PhosphoSiteiQ92750.

Expressioni

Tissue specificityi

Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.2 Publications

Gene expression databases

BgeeiQ92750.
CleanExiHS_TAF4B.
ExpressionAtlasiQ92750. baseline and differential.
GenevisibleiQ92750. HS.

Organism-specific databases

HPAiHPA028937.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription.3 Publications

GO - Molecular functioni

  • NF-kappaB binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112738. 11 interactions.
IntActiQ92750. 4 interactions.
STRINGi9606.ENSP00000269142.

Structurei

3D structure databases

ProteinModelPortaliQ92750.
SMRiQ92750. Positions 252-343, 654-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini256 – 35398TAFHPROSITE-ProRule annotationAdd
BLAST
Domaini653 – 70250Histone-foldAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni511 – 53323Required for interaction with P65/RELAAdd
BLAST
Regioni830 – 86233Required for interaction with TAF12Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili722 – 78766Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi516 – 55641Nuclear export signalAdd
BLAST

Sequence similaritiesi

Belongs to the TAF4 family.Curated
Contains 1 histone-fold domain.Curated
Contains 1 TAFH (NHR1) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2341. Eukaryota.
ENOG410XQS6. LUCA.
GeneTreeiENSGT00390000011620.
HOGENOMiHOG000154500.
HOVERGENiHBG103779.
InParanoidiQ92750.
KOiK03129.
OrthoDBiEOG7327N5.
PhylomeDBiQ92750.
TreeFamiTF316520.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007900. TAF4.
IPR003894. TAFH_NHR1.
[Graphical view]
PfamiPF05236. TAF4. 1 hit.
PF07531. TAFH. 1 hit.
[Graphical view]
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51119. TAFH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92750-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAGLTEPAG AAPPAAVSAS GTVTMAPAGA LPVRVESTPV ALGAVTKAPV
60 70 80 90 100
SVCVEPTASQ PLRSPVGTLV TKVAPVSAPP KVSSGPRLPA PQIVAVKAPN
110 120 130 140 150
TTTIQFPANL QLPPGTVLIK SNSGPLMLVS PQQTVTRAET TSNITSRPAV
160 170 180 190 200
PANPQTVKIC TVPNSSSQLI KKVAVTPVKK LAQIGTTVVT TVPKPSSVQS
210 220 230 240 250
VAVPTSVVTV TPGKPLNTVT TLKPSSLGAS STPSNEPNLK AENSAAVQIN
260 270 280 290 300
LSPTMLENVK KCKNFLAMLI KLACSGSQSP EMGQNVKKLV EQLLDAKIEA
310 320 330 340 350
EEFTRKLYVE LKSSPQPHLV PFLKKSVVAL RQLLPNSQSF IQQCVQQTSS
360 370 380 390 400
DMVIATCTTT VTTSPVVTTT VSSSQSEKSI IVSGATAPRT VSVQTLNPLA
410 420 430 440 450
GPVGAKAGVV TLHSVGPTAA TGGTTAGTGL LQTSKPLVTS VANTVTTVSL
460 470 480 490 500
QPEKPVVSGT AVTLSLPAVT FGETSGAAIC LPSVKPVVSS AGTTSDKPVI
510 520 530 540 550
GTPVQIKLAQ PGPVLSQPAG IPQAVQVKQL VVQQPSGGNE KQVTTISHSS
560 570 580 590 600
TLTIQKCGQK TMPVNTIIPT SQFPPASILK QITLPGNKIL SLQASPTQKN
610 620 630 640 650
RIKENVTSCF RDEDDINDVT SMAGVNLNEE NACILATNSE LVGTLIQSCK
660 670 680 690 700
DEPFLFIGAL QKRILDIGKK HDITELNSDA VNLISQATQE RLRGLLEKLT
710 720 730 740 750
AIAQHRMTTY KASENYILCS DTRSQLKFLE KLDQLEKQRK DLEEREMLLK
760 770 780 790 800
AAKSRSNKED PEQLRLKQKA KELQQLELAQ IQHRDANLTA LAAIGPRKKR
810 820 830 840 850
PLESGIEGLK DNLLASGTSS LTATKQLHRP RITRICLRDL IFCMEQEREM
860
KYSRALYLAL LK
Length:862
Mass (Da):91,091
Last modified:December 12, 2006 - v2
Checksum:iE380D5CD2445AC11
GO
Isoform 2 (identifier: Q92750-2) [UniParc]FASTAAdd to basket

Also known as: TAFII105-E9

The sequence of this isoform differs from the canonical sequence as follows:
     612-618: DEDDIND → STFICVY
     619-862: Missing.

Note: May be due to exon inclusion. No experimental confirmation available. Unphosphorylated form.
Show »
Length:618
Mass (Da):63,364
Checksum:i0EA429488262537F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti490 – 4967SAGTTSD → FCWDHIC in CAA70499 (PubMed:8858156).Curated
Sequence conflicti523 – 5308QAVQVKQL → TGSSSKQLFSLFH in CAA70499 (PubMed:8858156).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti249 – 2491I → V.
Corresponds to variant rs16942219 [ dbSNP | Ensembl ].
VAR_052259
Natural varianti539 – 5391N → S.
Corresponds to variant rs12963653 [ dbSNP | Ensembl ].
VAR_061836

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei612 – 6187DEDDIND → STFICVY in isoform 2. 1 PublicationVSP_022025
Alternative sequencei619 – 862244Missing in isoform 2. 1 PublicationVSP_022026Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY888048 mRNA. Translation: AAX14897.1.
AY888049 mRNA. Translation: AAX14898.1.
Y09321 mRNA. Translation: CAA70499.1.
CCDSiCCDS42421.1. [Q92750-1]
RefSeqiNP_001280654.1. NM_001293725.1.
NP_005631.1. NM_005640.2. [Q92750-1]
UniGeneiHs.369519.

Genome annotation databases

EnsembliENST00000269142; ENSP00000269142; ENSG00000141384. [Q92750-1]
ENST00000418698; ENSP00000389365; ENSG00000141384. [Q92750-2]
GeneIDi6875.
KEGGihsa:6875.
UCSCiuc002kvu.5. human. [Q92750-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY888048 mRNA. Translation: AAX14897.1.
AY888049 mRNA. Translation: AAX14898.1.
Y09321 mRNA. Translation: CAA70499.1.
CCDSiCCDS42421.1. [Q92750-1]
RefSeqiNP_001280654.1. NM_001293725.1.
NP_005631.1. NM_005640.2. [Q92750-1]
UniGeneiHs.369519.

3D structure databases

ProteinModelPortaliQ92750.
SMRiQ92750. Positions 252-343, 654-700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112738. 11 interactions.
IntActiQ92750. 4 interactions.
STRINGi9606.ENSP00000269142.

PTM databases

iPTMnetiQ92750.
PhosphoSiteiQ92750.

Polymorphism and mutation databases

BioMutaiTAF4B.
DMDMi119370533.

Proteomic databases

MaxQBiQ92750.
PaxDbiQ92750.
PRIDEiQ92750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000269142; ENSP00000269142; ENSG00000141384. [Q92750-1]
ENST00000418698; ENSP00000389365; ENSG00000141384. [Q92750-2]
GeneIDi6875.
KEGGihsa:6875.
UCSCiuc002kvu.5. human. [Q92750-1]

Organism-specific databases

CTDi6875.
GeneCardsiTAF4B.
HGNCiHGNC:11538. TAF4B.
HPAiHPA028937.
MalaCardsiTAF4B.
MIMi601689. gene.
615841. phenotype.
neXtProtiNX_Q92750.
Orphaneti399805. Male infertility with azoospermia or oligozoospermia due to single gene mutation.
PharmGKBiPA36313.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2341. Eukaryota.
ENOG410XQS6. LUCA.
GeneTreeiENSGT00390000011620.
HOGENOMiHOG000154500.
HOVERGENiHBG103779.
InParanoidiQ92750.
KOiK03129.
OrthoDBiEOG7327N5.
PhylomeDBiQ92750.
TreeFamiTF316520.

Enzyme and pathway databases

ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

GeneWikiiTAF4B.
GenomeRNAii6875.
PROiQ92750.
SOURCEiSearch...

Gene expression databases

BgeeiQ92750.
CleanExiHS_TAF4B.
ExpressionAtlasiQ92750. baseline and differential.
GenevisibleiQ92750. HS.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007900. TAF4.
IPR003894. TAFH_NHR1.
[Graphical view]
PfamiPF05236. TAF4. 1 hit.
PF07531. TAFH. 1 hit.
[Graphical view]
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51119. TAFH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cyclic AMP-dependent modification of gonad-selective TAF(II)105 in a human ovarian granulosa cell line."
    Wu Y., Lu Y., Hu Y., Li R.
    J. Cell. Biochem. 96:751-759(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Ovarian carcinoma.
  2. "Human TAFII 105 is a cell type-specific TFIID subunit related to hTAFII130."
    Dikstein R., Zhou S., Tjian R.
    Cell 87:137-146(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 67-862 (ISOFORM 1), PROTEIN SEQUENCE OF 81-85; 242-253; 281-290; 302-310; 377-388; 473-479; 507-522; 580-587; 675-689; 697-712 AND 746-800, TISSUE SPECIFICITY.
  3. "Specific interaction of TAFII105 with OCA-B is involved in activation of octamer-dependent transcription."
    Wolstein O., Silkov A., Revach M., Dikstein R.
    J. Biol. Chem. 275:16459-16465(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POU2AF1, FUNCTION.
  4. "Interaction of TAFII105 with selected p65/RelA dimers is associated with activation of subset of NF-kappa B genes."
    Yamit-Hezi A., Nir S., Wolstein O., Dikstein R.
    J. Biol. Chem. 275:18180-18187(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH P65/RELA AND REL, FUNCTION.
  5. "A composite nuclear export signal in the TBP-associated factor TAFII105."
    Rashevsky-Finkel A., Silkov A., Dikstein R.
    J. Biol. Chem. 276:44963-44969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL.
  6. Cited for: INTERACTION WITH TAF12 IN A COMPLEX WITH TAF6; TAF9; TAF12 AND TAF4B, DNA-BINDING.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Truncating mutations in TAF4B and ZMYND15 causing recessive azoospermia."
    Ayhan O., Balkan M., Guven A., Hazan R., Atar M., Tok A., Tolun A.
    J. Med. Genet. 51:239-244(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN SPGF13, POSSIBLE FUNCTION.

Entry informationi

Entry nameiTAF4B_HUMAN
AccessioniPrimary (citable) accession number: Q92750
Secondary accession number(s): Q29YA4, Q29YA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 12, 2006
Last modified: June 8, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.