Q92738 (US6NL_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 88.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: USP6 N-terminal-like protein Alternative name(s): Related to the N-terminus of tre Short name=RN-tre | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 828 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as a GTPase-activating protein for RAB5A. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Ref.9 |
| Subunit structure | Interacts with EPS8. Ref.1 |
| Tissue specificity | Widely expressed. Ref.1 |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 |
| Sequence similarities | Contains 1 Rab-GAP TBC domain. |
| Sequence caution | The sequence BAA02807.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Molecular function | GTPase activation |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Cellular component | intracellular Inferred from electronic annotation. Source: InterPro |
| Molecular function | Rab GTPase activator activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q92738-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q92738-2) The sequence of this isoform differs from the canonical sequence as follows: 1-2: MN → MRTEAVVNSQGQTDYLTKD |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 828 | 828 | USP6 N-terminal-like protein | PRO_0000208056 | |||||
Regions | |||||||||
| Domain | 100 – 292 | 193 | Rab-GAP TBC | ||||||
Amino acid modifications | |||||||||
| Modified residue | 391 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 396 | 1 | Phosphoserine Ref.13 | ||||||
| Modified residue | 546 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 582 | 1 | Phosphotyrosine Ref.10 | ||||||
| Modified residue | 585 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 617 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 673 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 680 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 710 | 1 | Phosphotyrosine Ref.10 | ||||||
| Modified residue | 716 | 1 | Phosphoserine Ref.10 Ref.13 Ref.14 Ref.15 | ||||||
| Modified residue | 729 | 1 | Phosphotyrosine Ref.10 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 2 | 2 | MN → MRTEAVVNSQGQTDYLTKD in isoform 2. | VSP_040850 | |||||
Experimental info | |||||||||
| Mutagenesis | 106 | 1 | R → A: Loss of GAP activity on RAB5A. Ref.9 | ||||||
| Mutagenesis | 147 | 1 | D → A: Loss of GAP activity on RAB5A. Ref.9 | ||||||
| Mutagenesis | 150 | 1 | R → A: Loss of GAP activity on RAB5A. Ref.9 | ||||||
| Sequence conflict | 622 | 1 | E → D in BAF85633. Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain." Wong W.T., Seki N., Nagase T., Nomura N., Robbins K.C., Di Fiore P.P., Matoskova B. Oncogene 12:2563-2571(1996) [PubMed: 8700515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH EPS8. |
| [2] | "Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity." Ishibashi K., Kanno E., Itoh T., Fukuda M. Genes Cells 14:41-52(2009) [PubMed: 19077034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [3] | "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow. |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea. |
| [5] | "The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. Rogers J.Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis. |
| [8] | "Normalization and subtraction: two approaches to facilitate gene discovery." Bonaldo M.F., Lennon G., Soares M.B. Genome Res. 6:791-806(1996) [PubMed: 8889548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-202 (ISOFORM 2). |
| [9] | "The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5." Lanzetti L., Rybin V., Malabarba M.G., Christoforidis S., Scita G., Zerial M., Di Fiore P.P. Nature 408:374-377(2000) [PubMed: 11099046] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-106; ASP-147 AND ARG-150. |
| [10] | "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-582; SER-585; TYR-710; SER-716 AND TYR-729, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [11] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [12] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [13] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-396 AND SER-716, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [14] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [15] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB449916 mRNA. Translation: BAH16659.1. D13644 mRNA. Translation: BAA02807.2. Different initiation. AK292944 mRNA. Translation: BAF85633.1. AL512631, AL590416 Genomic DNA. Translation: CAH70484.1. AL590416, AL512631 Genomic DNA. Translation: CAI16966.1. CH471072 Genomic DNA. Translation: EAW86344.1. BC042943 mRNA. Translation: AAH42943.1. CF121539 mRNA. No translation available. |
| IPI | IPI00400875. IPI00921527. |
| RefSeq | NP_001073960.1. NM_001080491.2. NP_055503.1. NM_014688.2. |
| UniGene | Hs.498661. |
3D structure databases | |
| ProteinModelPortal | Q92738. |
| SMR | Q92738. Positions 72-341. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q92738. |
PTM databases | |
| PhosphoSite | Q92738. |
Polymorphism databases | |
| DMDM | 50897492. |
Proteomic databases | |
| PRIDE | Q92738. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000379237; ENSP00000368539; ENSG00000148429. |
| GeneID | 9712. |
| KEGG | hsa:9712. |
| UCSC | uc001ikt.2. human. |
Organism-specific databases | |
| CTD | 9712. |
| GeneCards | GC10M011542. |
| H-InvDB | HIX0008637. |
| HGNC | HGNC:16858. USP6NL. |
| HPA | HPA039196. |
| MIM | 605405. gene. |
| neXtProt | NX_Q92738. |
| PharmGKB | PA134955568. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG08583. |
| GeneTree | ENSGT00550000074322. |
| HOVERGEN | HBG059159. |
| InParanoid | Q92738. |
| OMA | HGNSPKH. |
| OrthoDB | EOG4NKBVT. |
| PhylomeDB | Q92738. |
Gene expression databases | |
| ArrayExpress | Q92738. |
| Bgee | Q92738. |
| CleanEx | HS_USP6NL. |
| Genevestigator | Q92738. |
| GermOnline | ENSG00000148429. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000195. RabGAP/TBC_dom. [Graphical view] |
| Pfam | PF00566. TBC. 1 hit. [Graphical view] |
| SMART | SM00164. TBC. 1 hit. [Graphical view] |
| SUPFAM | SSF47923. RabGAP_TBC. 2 hits. |
| PROSITE | PS50086. TBC_RABGAP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| SOURCE | Search... |
Entry information
| Entry name | US6NL_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q92738 Secondary accession number(s): A8KA79 Q7L0K9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 10 Human chromosome 10: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with