Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q92738 (US6NL_HUMAN)

Last modified January 19, 2010. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    USP6 N-terminal-like protein
Alternative name(s):
    Related to the N-terminus of tre
      Short name=RN-tre
Gene names
Name: USP6NL
Synonyms: KIAA0019
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a GTPase-activating protein for RAB5A. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Ref.4

Subunit structure

Interacts with EPS8. Ref.1

Tissue specificity

Widely expressed. Ref.1

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Sequence similarities

Contains 1 Rab-GAP TBC domain.

Ontologies

Keywords
   Molecular functionGTPase activation
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processregulation of Rab GTPase activity

Inferred from electronic annotation. Source: InterPro

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionRab GTPase activator activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 828828USP6 N-terminal-like protein
PRO_0000208056

Regions

Domain100 – 292193Rab-GAP TBC

Amino acid modifications

Modified residue3911Phosphoserine Ref.8
Modified residue3961Phosphoserine Ref.8
Modified residue5461Phosphoserine By similarity
Modified residue5821Phosphotyrosine Ref.5
Modified residue5851Phosphoserine Ref.5
Modified residue6171Phosphoserine Ref.7
Modified residue6731Phosphoserine By similarity
Modified residue6801Phosphoserine Ref.6
Modified residue7101Phosphotyrosine Ref.5
Modified residue7161Phosphoserine Ref.5 Ref.8 Ref.9 Ref.10
Modified residue7291Phosphotyrosine Ref.5

Experimental info

Mutagenesis1061R → A: Loss of GAP activity on RAB5A. Ref.4
Mutagenesis1471D → A: Loss of GAP activity on RAB5A. Ref.4
Mutagenesis1501R → A: Loss of GAP activity on RAB5A. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q92738-1 [UniParc].

Last modified July 30, 2004. Version 3.
Checksum: A5E3DBF0FEE04E78

FASTA82894,104
        10         20         30         40         50         60 
MNSDQDVALK LAQERAEIVA KYDRGREGAE IEPWEDADYL VYKVTDRFGF LHEEELPDHN 

        70         80         90        100        110        120 
VAVERQKHLE IERTTKWLKM LKGWEKYKNT EKFHRRIYKG IPLQLRGEVW ALLLEIPKMK 

       130        140        150        160        170        180 
EETRDLYSKL KHRARGCSPD IRQIDLDVNR TFRDHIMFRD RYGVKQQSLF HVLAAYSIYN 

       190        200        210        220        230        240 
TEVGYCQGMS QITALLLMYM NEEDAFWALV KLFSGPKHAM HGFFVQGFPK LLRFQEHHEK 

       250        260        270        280        290        300 
ILNKFLSKLK QHLDSQEIYT SFYTMKWFFQ CFLDRTPFTL NLRIWDIYIF EGERVLTAMS 

       310        320        330        340        350        360 
YTILKLHKKH LMKLSMEELV EFFQETLAKD FFFEDDFVIE QLQISMTELK RAKLDLPEPG 

       370        380        390        400        410        420 
KEDEYPKKPL GQLPPELQSW GVHHLSNGQR SVGRPSPLAS GRRESGAPHR RHEHSPHPQS 

       430        440        450        460        470        480 
RTGTPERAQP PRRKSVEEES KKLKDEADFQ RKLPSGPQDS SRQYNHAAAN QNSNATSNIR 

       490        500        510        520        530        540 
KEFVPKWNKP SDVSATERTA KYTMEGKGRA AHPALAVTVP GPAEVRVSNV RPKMKALDAE 

       550        560        570        580        590        600 
DGKRGSTASQ YDNVPGPELD SGASVEEALE RAYSQSPRHA LYPPSPRKHA EPSSSPSKVS 

       610        620        630        640        650        660 
NKFTFKVQPP SHARYPSQLD GEARGLAHPP SYSNPPVYHG NSPKHFPTAN SSFASPQFSP 

       670        680        690        700        710        720 
GTQLNPSRRP HGSTLSVSAS PEKSYSRPSP LVLPSSRIEV LPVDTGAGGY SGNSGSPKNG 

       730        740        750        760        770        780 
KLIIPPVDYL PDNRTWSEVS YTYRPETQGQ SWTRDASRGN LPKYSAFQLA PFQDHGLPAV 

       790        800        810        820 
SVDSPVRYKA SPAAEDASPS GYPYSGPPPP AYHYRNRDGL SIQESVLL 

« Hide

References

« Hide 'large scale' references
[1]"RN-tre identifies a family of tre-related proteins displaying a novel potential protein binding domain."
Wong W.T., Seki N., Nagase T., Nomura N., Robbins K.C., Di Fiore P.P., Matoskova B.
Oncogene 12:2563-2571(1996) [PubMed: 8700515] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH EPS8.
[2]"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1."
Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S.
DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone marrow.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5."
Lanzetti L., Rybin V., Malabarba M.G., Christoforidis S., Scita G., Zerial M., Di Fiore P.P.
Nature 408:374-377(2000) [PubMed: 11099046] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ARG-106; ASP-147 AND ARG-150.
[5]"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-582; SER-585; TYR-710; SER-716 AND TYR-729, MASS SPECTROMETRY.
Tissue: T-cell.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, MASS SPECTROMETRY.
Tissue: Epithelium.
[7]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, MASS SPECTROMETRY.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-396 AND SER-716, MASS SPECTROMETRY.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, MASS SPECTROMETRY.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13644 mRNA. Translation: BAA02807.2. Different initiation.
BC042943 mRNA. Translation: AAH42943.1.
IPIIPI00921527.
RefSeqNP_001073960.1.
NP_055503.1.
UniGeneHs.498661

3D structure databases

SMRQ92738. Positions 70-326.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ92738.

PTM databases

PhosphoSiteQ92738.

Proteomic databases

PRIDEQ92738.

Genome annotation databases

EnsemblENST00000379237; ENSP00000368539; ENSG00000148429; Homo sapiens. [Genome view]
GeneID9712.
KEGGhsa:9712.
UCSCuc001ikt.2. human.

Organism-specific databases

CTD9712.
GeneCardsGC10M011542.
H-InvDBHIX0008637.
HGNCHGNC:16858. USP6NL.
MIM605405. gene.
PharmGKBPA134955568.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG08583.
HOVERGENQ92738.
InParanoidQ92738.
PhylomeDBQ92738.

Gene expression databases

ArrayExpressQ92738.
BgeeQ92738.
CleanExHS_USP6NL.
GenevestigatorQ92738.
GermOnlineENSG00000148429. Homo sapiens.

Family and domain databases

InterProIPR000195. RabGAP_TBC.
[Graphical view]
PfamPF00566. TBC. 1 hit.
[Graphical view]
SMARTSM00164. TBC. 1 hit.
[Graphical view]
PROSITEPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameUS6NL_HUMAN
AccessionPrimary (citable) accession number: Q92738
Secondary accession number(s): Q15400, Q7L0K9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 30, 2004
Last sequence update: July 30, 2004
Last modified: January 19, 2010
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents