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Protein

USP6 N-terminal-like protein

Gene

USP6NL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex.3 Publications

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • Rab GTPase binding Source: UniProtKB

GO - Biological processi

  • activation of GTPase activity Source: GO_Central
  • Golgi organization Source: UniProtKB
  • intracellular protein transport Source: GO_Central
  • plasma membrane to endosome transport Source: UniProtKB
  • regulation of Golgi organization Source: UniProtKB
  • regulation of vesicle fusion Source: GO_Central
  • retrograde transport, plasma membrane to Golgi Source: UniProtKB
  • virion assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148429-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
SignaLinkiQ92738.

Names & Taxonomyi

Protein namesi
Recommended name:
USP6 N-terminal-like protein
Alternative name(s):
Related to the N-terminus of tre
Short name:
RN-tre
Gene namesi
Name:USP6NL
Synonyms:KIAA0019
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:16858. USP6NL.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: UniProtKB
  • cytosol Source: Reactome
  • endomembrane system Source: GO_Central
  • plasma membrane Source: UniProtKB
  • trans-Golgi network membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106R → A: Loss of GAP activity on RAB5A. 1 Publication1
Mutagenesisi147D → A: Loss of GAP activity on RAB5A. 1 Publication1
Mutagenesisi150R → A: Loss of GAP activity on RAB5A. 2 Publications1

Organism-specific databases

DisGeNETi9712.
OpenTargetsiENSG00000148429.
PharmGKBiPA134955568.

Polymorphism and mutation databases

BioMutaiUSP6NL.
DMDMi50897492.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080561 – 828USP6 N-terminal-like proteinAdd BLAST828

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei391PhosphoserineCombined sources1
Modified residuei396PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei546PhosphoserineBy similarity1
Modified residuei549PhosphoserineBy similarity1
Modified residuei582PhosphotyrosineCombined sources1
Modified residuei585PhosphoserineCombined sources1
Modified residuei642PhosphoserineCombined sources1
Modified residuei655PhosphoserineCombined sources1
Modified residuei659PhosphoserineCombined sources1
Modified residuei676PhosphoserineBy similarity1
Modified residuei680PhosphoserineCombined sources1
Modified residuei716PhosphoserineCombined sources1
Modified residuei729PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ92738.
MaxQBiQ92738.
PaxDbiQ92738.
PeptideAtlasiQ92738.
PRIDEiQ92738.

PTM databases

iPTMnetiQ92738.
PhosphoSitePlusiQ92738.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000148429.
CleanExiHS_USP6NL.
ExpressionAtlasiQ92738. baseline and differential.
GenevisibleiQ92738. HS.

Organism-specific databases

HPAiHPA039196.

Interactioni

Subunit structurei

Interacts with EPS8.1 Publication

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115063. 18 interactors.
IntActiQ92738. 10 interactors.
MINTiMINT-1518588.
STRINGi9606.ENSP00000277575.

Structurei

3D structure databases

ProteinModelPortaliQ92738.
SMRiQ92738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 292Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST193

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1102. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00860000133698.
HOGENOMiHOG000139916.
HOVERGENiHBG059159.
InParanoidiQ92738.
KOiK20133.
PhylomeDBiQ92738.
TreeFamiTF318099.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92738-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSDQDVALK LAQERAEIVA KYDRGREGAE IEPWEDADYL VYKVTDRFGF
60 70 80 90 100
LHEEELPDHN VAVERQKHLE IERTTKWLKM LKGWEKYKNT EKFHRRIYKG
110 120 130 140 150
IPLQLRGEVW ALLLEIPKMK EETRDLYSKL KHRARGCSPD IRQIDLDVNR
160 170 180 190 200
TFRDHIMFRD RYGVKQQSLF HVLAAYSIYN TEVGYCQGMS QITALLLMYM
210 220 230 240 250
NEEDAFWALV KLFSGPKHAM HGFFVQGFPK LLRFQEHHEK ILNKFLSKLK
260 270 280 290 300
QHLDSQEIYT SFYTMKWFFQ CFLDRTPFTL NLRIWDIYIF EGERVLTAMS
310 320 330 340 350
YTILKLHKKH LMKLSMEELV EFFQETLAKD FFFEDDFVIE QLQISMTELK
360 370 380 390 400
RAKLDLPEPG KEDEYPKKPL GQLPPELQSW GVHHLSNGQR SVGRPSPLAS
410 420 430 440 450
GRRESGAPHR RHEHSPHPQS RTGTPERAQP PRRKSVEEES KKLKDEADFQ
460 470 480 490 500
RKLPSGPQDS SRQYNHAAAN QNSNATSNIR KEFVPKWNKP SDVSATERTA
510 520 530 540 550
KYTMEGKGRA AHPALAVTVP GPAEVRVSNV RPKMKALDAE DGKRGSTASQ
560 570 580 590 600
YDNVPGPELD SGASVEEALE RAYSQSPRHA LYPPSPRKHA EPSSSPSKVS
610 620 630 640 650
NKFTFKVQPP SHARYPSQLD GEARGLAHPP SYSNPPVYHG NSPKHFPTAN
660 670 680 690 700
SSFASPQFSP GTQLNPSRRP HGSTLSVSAS PEKSYSRPSP LVLPSSRIEV
710 720 730 740 750
LPVDTGAGGY SGNSGSPKNG KLIIPPVDYL PDNRTWSEVS YTYRPETQGQ
760 770 780 790 800
SWTRDASRGN LPKYSAFQLA PFQDHGLPAV SVDSPVRYKA SPAAEDASPS
810 820
GYPYSGPPPP AYHYRNRDGL SIQESVLL
Length:828
Mass (Da):94,104
Last modified:July 30, 2004 - v3
Checksum:iA5E3DBF0FEE04E78
GO
Isoform 2 (identifier: Q92738-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MN → MRTEAVVNSQGQTDYLTKD

Show »
Length:845
Mass (Da):95,997
Checksum:i3C174D986C902346
GO

Sequence cautioni

The sequence BAA02807 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti622E → D in BAF85633 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0408501 – 2MN → MRTEAVVNSQGQTDYLTKD in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449916 mRNA. Translation: BAH16659.1.
D13644 mRNA. Translation: BAA02807.2. Different initiation.
AK292944 mRNA. Translation: BAF85633.1.
AL512631, AL590416 Genomic DNA. Translation: CAH70484.1.
AL590416, AL512631 Genomic DNA. Translation: CAI16966.1.
CH471072 Genomic DNA. Translation: EAW86344.1.
BC042943 mRNA. Translation: AAH42943.1.
CF121539 mRNA. No translation available.
CCDSiCCDS44357.1. [Q92738-2]
CCDS53492.1. [Q92738-1]
RefSeqiNP_001073960.1. NM_001080491.3. [Q92738-2]
NP_055503.1. NM_014688.3. [Q92738-1]
UniGeneiHs.498661.
Hs.688100.

Genome annotation databases

EnsembliENST00000277575; ENSP00000277575; ENSG00000148429. [Q92738-2]
ENST00000609104; ENSP00000476462; ENSG00000148429. [Q92738-1]
GeneIDi9712.
KEGGihsa:9712.
UCSCiuc001iks.1. human. [Q92738-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449916 mRNA. Translation: BAH16659.1.
D13644 mRNA. Translation: BAA02807.2. Different initiation.
AK292944 mRNA. Translation: BAF85633.1.
AL512631, AL590416 Genomic DNA. Translation: CAH70484.1.
AL590416, AL512631 Genomic DNA. Translation: CAI16966.1.
CH471072 Genomic DNA. Translation: EAW86344.1.
BC042943 mRNA. Translation: AAH42943.1.
CF121539 mRNA. No translation available.
CCDSiCCDS44357.1. [Q92738-2]
CCDS53492.1. [Q92738-1]
RefSeqiNP_001073960.1. NM_001080491.3. [Q92738-2]
NP_055503.1. NM_014688.3. [Q92738-1]
UniGeneiHs.498661.
Hs.688100.

3D structure databases

ProteinModelPortaliQ92738.
SMRiQ92738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115063. 18 interactors.
IntActiQ92738. 10 interactors.
MINTiMINT-1518588.
STRINGi9606.ENSP00000277575.

PTM databases

iPTMnetiQ92738.
PhosphoSitePlusiQ92738.

Polymorphism and mutation databases

BioMutaiUSP6NL.
DMDMi50897492.

Proteomic databases

EPDiQ92738.
MaxQBiQ92738.
PaxDbiQ92738.
PeptideAtlasiQ92738.
PRIDEiQ92738.

Protocols and materials databases

DNASUi9712.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000277575; ENSP00000277575; ENSG00000148429. [Q92738-2]
ENST00000609104; ENSP00000476462; ENSG00000148429. [Q92738-1]
GeneIDi9712.
KEGGihsa:9712.
UCSCiuc001iks.1. human. [Q92738-1]

Organism-specific databases

CTDi9712.
DisGeNETi9712.
GeneCardsiUSP6NL.
HGNCiHGNC:16858. USP6NL.
HPAiHPA039196.
MIMi605405. gene.
neXtProtiNX_Q92738.
OpenTargetsiENSG00000148429.
PharmGKBiPA134955568.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1102. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00860000133698.
HOGENOMiHOG000139916.
HOVERGENiHBG059159.
InParanoidiQ92738.
KOiK20133.
PhylomeDBiQ92738.
TreeFamiTF318099.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000148429-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
SignaLinkiQ92738.

Miscellaneous databases

ChiTaRSiUSP6NL. human.
GeneWikiiUSP6NL.
GenomeRNAii9712.
PROiQ92738.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148429.
CleanExiHS_USP6NL.
ExpressionAtlasiQ92738. baseline and differential.
GenevisibleiQ92738. HS.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUS6NL_HUMAN
AccessioniPrimary (citable) accession number: Q92738
Secondary accession number(s): A8KA79
, Q15400, Q5VV10, Q7L0K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 30, 2004
Last sequence update: July 30, 2004
Last modified: November 30, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.