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Protein

DNA repair and recombination protein RAD54-like

Gene

RAD54L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi183 – 190ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • annealing helicase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW

GO - Biological processi

  • chromosome organization Source: Ensembl
  • determination of adult lifespan Source: Ensembl
  • DNA recombination Source: ProtInc
  • DNA repair Source: ProtInc
  • double-strand break repair via homologous recombination Source: Ensembl
  • meiotic nuclear division Source: ProtInc
  • response to drug Source: Ensembl
  • response to ionizing radiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000085999-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD54-like (EC:3.6.4.-)
Alternative name(s):
RAD54 homolog
Short name:
hHR54
Short name:
hRAD54
Gene namesi
Name:RAD54L
Synonyms:RAD54A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9826. RAD54L.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi189K → R: Unable to rescue the MMS-sensitive phenotype of S cerevisiae rad54delta cells. 1 Publication1

Organism-specific databases

DisGeNETi8438.
MalaCardsiRAD54L.
OpenTargetsiENSG00000085999.
PharmGKBiPA34181.

Chemistry databases

ChEMBLiCHEMBL2146297.

Polymorphism and mutation databases

BioMutaiRAD54L.
DMDMi51316508.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743371 – 747DNA repair and recombination protein RAD54-likeAdd BLAST747

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ92698.
MaxQBiQ92698.
PaxDbiQ92698.
PeptideAtlasiQ92698.
PRIDEiQ92698.

PTM databases

iPTMnetiQ92698.
PhosphoSitePlusiQ92698.

Expressioni

Inductioni

Expression increases approximately 3-fold in late G1 phase compared to other phases of the cell cycle.

Gene expression databases

BgeeiENSG00000085999.
CleanExiHS_RAD54L.
ExpressionAtlasiQ92698. baseline and differential.
GenevisibleiQ92698. HS.

Organism-specific databases

HPAiHPA028954.
HPA051537.

Interactioni

Subunit structurei

Interacts (via N-terminus) with RAD51.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TNKS2Q9H2K23EBI-5333483,EBI-4398527
WDYHV1Q96HA83EBI-5333483,EBI-741158

Protein-protein interaction databases

BioGridi114018. 7 interactors.
DIPiDIP-48628N.
IntActiQ92698. 7 interactors.
STRINGi9606.ENSP00000361043.

Chemistry databases

BindingDBiQ92698.

Structurei

3D structure databases

ProteinModelPortaliQ92698.
SMRiQ92698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 345Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini500 – 653Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 9Required for chromatin remodeling, strand pairing activities and coupling of ATPase activityBy similarity8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 299DEGH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0390. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074619.
HOGENOMiHOG000204521.
HOVERGENiHBG058654.
InParanoidiQ92698.
KOiK10875.
OMAiVTRPVII.
OrthoDBiEOG091G01Z3.
PhylomeDBiQ92698.
TreeFamiTF101224.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRSLAPSQL AKRKPEGRSC DDEDWQPGLV TPRKRKSSSE TQIQECFLSP
60 70 80 90 100
FRKPLSQLTN QPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL
110 120 130 140 150
KRAGVRRALH DPLEKDALVL YEPPPLSAHD QLKLDKEKLP VHVVVDPILS
160 170 180 190 200
KVLRPHQREG VKFLWECVTS RRIPGSHGCI MADEMGLGKT LQCITLMWTL
210 220 230 240 250
LRQSPECKPE IDKAVVVSPS SLVKNWYNEV GKWLGGRIQP LAIDGGSKDE
260 270 280 290 300
IDQKLEGFMN QRGARVSSPI LIISYETFRL HVGVLQKGSV GLVICDEGHR
310 320 330 340 350
LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA
360 370 380 390 400
HEFKKHFELP ILKGRDAAAS EADRQLGEER LRELTSIVNR CLIRRTSDIL
410 420 430 440 450
SKYLPVKIEQ VVCCRLTPLQ TELYKRFLRQ AKPAEELLEG KMSVSSLSSI
460 470 480 490 500
TSLKKLCNHP ALIYDKCVEE EDGFVGALDL FPPGYSSKAL EPQLSGKMLV
510 520 530 540 550
LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRARRYLY VRLDGTMSIK
560 570 580 590 600
KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND
610 620 630 640 650
EQAMARVWRD GQKKTCYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ
660 670 680 690 700
DVERHFSLGE LKELFILDEA SLSDTHDRLH CRRCVNSRQI RPPPDGSDCT
710 720 730 740
SDLAGWNHCT DKWGLRDEVL QAAWDAASTA ITFVFHQRSH EEQRGLR
Length:747
Mass (Da):84,352
Last modified:August 16, 2004 - v2
Checksum:i718100974CA6687B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti738R → H in CAA66379 (PubMed:8805304).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05536321D → G.1 PublicationCorresponds to variant rs28363192dbSNPEnsembl.1
Natural variantiVAR_01955963P → H in a colon adenocarcinoma sample. 1 PublicationCorresponds to variant rs121908688dbSNPEnsembl.1
Natural variantiVAR_05536474I → M.1 PublicationCorresponds to variant rs28363209dbSNPEnsembl.1
Natural variantiVAR_019560151K → E.1 PublicationCorresponds to variant rs2295466dbSNPEnsembl.1
Natural variantiVAR_055365202R → C.1 PublicationCorresponds to variant rs28363218dbSNPEnsembl.1
Natural variantiVAR_019561325G → R in a breast cancer sample; invasive ductal. 1 PublicationCorresponds to variant rs121908690dbSNPEnsembl.1
Natural variantiVAR_055366380R → Q.1 PublicationCorresponds to variant rs28363234dbSNPEnsembl.1
Natural variantiVAR_019562444V → E in a non-Hodgkin lymphoma sample. 1 PublicationCorresponds to variant rs121908689dbSNPEnsembl.1
Natural variantiVAR_055367534R → C.1 PublicationCorresponds to variant rs28363240dbSNPEnsembl.1
Natural variantiVAR_055368583I → T.1 PublicationCorresponds to variant rs28363243dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97795 mRNA. Translation: CAA66379.1.
AY623117 Genomic DNA. Translation: AAT38113.1.
AL121602 Genomic DNA. Translation: CAI22117.1.
BC121059 mRNA. Translation: AAI21060.1.
BC121060 mRNA. Translation: AAI21061.1.
CCDSiCCDS532.1.
RefSeqiNP_001136020.1. NM_001142548.1.
NP_003570.2. NM_003579.3.
UniGeneiHs.642042.

Genome annotation databases

EnsembliENST00000371975; ENSP00000361043; ENSG00000085999.
ENST00000442598; ENSP00000396113; ENSG00000085999.
GeneIDi8438.
KEGGihsa:8438.
UCSCiuc001cpl.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97795 mRNA. Translation: CAA66379.1.
AY623117 Genomic DNA. Translation: AAT38113.1.
AL121602 Genomic DNA. Translation: CAI22117.1.
BC121059 mRNA. Translation: AAI21060.1.
BC121060 mRNA. Translation: AAI21061.1.
CCDSiCCDS532.1.
RefSeqiNP_001136020.1. NM_001142548.1.
NP_003570.2. NM_003579.3.
UniGeneiHs.642042.

3D structure databases

ProteinModelPortaliQ92698.
SMRiQ92698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114018. 7 interactors.
DIPiDIP-48628N.
IntActiQ92698. 7 interactors.
STRINGi9606.ENSP00000361043.

Chemistry databases

BindingDBiQ92698.
ChEMBLiCHEMBL2146297.

PTM databases

iPTMnetiQ92698.
PhosphoSitePlusiQ92698.

Polymorphism and mutation databases

BioMutaiRAD54L.
DMDMi51316508.

Proteomic databases

EPDiQ92698.
MaxQBiQ92698.
PaxDbiQ92698.
PeptideAtlasiQ92698.
PRIDEiQ92698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371975; ENSP00000361043; ENSG00000085999.
ENST00000442598; ENSP00000396113; ENSG00000085999.
GeneIDi8438.
KEGGihsa:8438.
UCSCiuc001cpl.3. human.

Organism-specific databases

CTDi8438.
DisGeNETi8438.
GeneCardsiRAD54L.
HGNCiHGNC:9826. RAD54L.
HPAiHPA028954.
HPA051537.
MalaCardsiRAD54L.
MIMi603615. gene.
neXtProtiNX_Q92698.
OpenTargetsiENSG00000085999.
PharmGKBiPA34181.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0390. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00550000074619.
HOGENOMiHOG000204521.
HOVERGENiHBG058654.
InParanoidiQ92698.
KOiK10875.
OMAiVTRPVII.
OrthoDBiEOG091G01Z3.
PhylomeDBiQ92698.
TreeFamiTF101224.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000085999-MONOMER.

Miscellaneous databases

ChiTaRSiRAD54L. human.
GeneWikiiDNA_repair_and_recombination_protein_RAD54-like.
GenomeRNAii8438.
PROiQ92698.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000085999.
CleanExiHS_RAD54L.
ExpressionAtlasiQ92698. baseline and differential.
GenevisibleiQ92698. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD54_HUMAN
AccessioniPrimary (citable) accession number: Q92698
Secondary accession number(s): Q5TE31, Q6IUY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.