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Protein

Acidic leucine-rich nuclear phosphoprotein 32 family member B

Gene

ANP32B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.By similarity1 Publication

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • RNA polymerase binding Source: UniProtKB

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • histone exchange Source: GO_Central
  • inner ear development Source: Ensembl
  • negative regulation of cell differentiation Source: UniProtKB
  • nucleosome assembly Source: UniProtKB
  • palate development Source: Ensembl
  • positive regulation of protein export from nucleus Source: UniProtKB
  • vasculature development Source: Ensembl
  • ventricular system development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136938-MONOMER.
SIGNORiQ92688.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic leucine-rich nuclear phosphoprotein 32 family member B
Alternative name(s):
Acidic protein rich in leucines
Putative HLA-DR-associated protein I-2
Short name:
PHAPI2
Silver-stainable protein SSP29
Gene namesi
Name:ANP32B
Synonyms:APRIL, PHAPI2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:16677. ANP32B.

Subcellular locationi

Isoform 1 :
  • Nucleus

  • Note: Accumulates in the nuclei at the S phase.By similarity
Isoform 2 :
  • Cytoplasm

  • Note: Lacks a nuclear localization signal.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10541.
OpenTargetsiENSG00000136938.
PharmGKBiPA24812.

Polymorphism and mutation databases

BioMutaiANP32B.
DMDMi26390818.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001375951 – 251Acidic leucine-rich nuclear phosphoprotein 32 family member BAdd BLAST251

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86N6-acetyllysineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei244PhosphothreonineCombined sources1

Post-translational modificationi

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ92688.
MaxQBiQ92688.
PaxDbiQ92688.
PeptideAtlasiQ92688.
PRIDEiQ92688.
TopDownProteomicsiQ92688-1. [Q92688-1]
Q92688-2. [Q92688-2]

PTM databases

iPTMnetiQ92688.
PhosphoSitePlusiQ92688.
SwissPalmiQ92688.

Miscellaneous databases

PMAP-CutDBQ92688.

Expressioni

Tissue specificityi

Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta.1 Publication

Gene expression databases

BgeeiENSG00000136938.
CleanExiHS_ANP32B.
GenevisibleiQ92688. HS.

Organism-specific databases

HPAiHPA063133.
HPA067561.

Interactioni

Subunit structurei

Monomer. Interacts with histones H3 and H4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KPNA1P522947EBI-762428,EBI-358383
KPNA5O151313EBI-762428,EBI-540602
MEOX2A4D1273EBI-762428,EBI-10172134
SDCBPO005605EBI-762428,EBI-727004

GO - Molecular functioni

  • histone binding Source: UniProtKB
  • RNA polymerase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi115795. 54 interactors.
IntActiQ92688. 34 interactors.
STRINGi9606.ENSP00000345848.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Helixi16 – 18Combined sources3
Beta strandi20 – 23Combined sources4
Helixi39 – 43Combined sources5
Beta strandi46 – 52Combined sources7
Beta strandi68 – 73Combined sources6
Helixi82 – 86Combined sources5
Beta strandi92 – 94Combined sources3
Beta strandi96 – 99Combined sources4
Helixi104 – 109Combined sources6
Beta strandi117 – 119Combined sources3
Helixi124 – 126Combined sources3
Helixi131 – 136Combined sources6
Beta strandi157 – 159Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ELLNMR-A1-161[»]
2RR6NMR-A1-161[»]
ProteinModelPortaliQ92688.
SMRiQ92688.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92688.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati16 – 40LRR 11 PublicationAdd BLAST25
Repeati43 – 64LRR 21 PublicationAdd BLAST22
Repeati65 – 84LRR 31 PublicationAdd BLAST20
Repeati89 – 110LRR 41 PublicationAdd BLAST22
Domaini123 – 161LRRCTAdd BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi146 – 251Asp/Glu-rich (highly acidic)Add BLAST106

Domaini

Histone binding is mediated by the concave surface of the LRR region.

Sequence similaritiesi

Belongs to the ANP32 family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiQ92688.
KOiK18647.
OMAiLINVGLM.
OrthoDBiEOG091G0R8Z.
PhylomeDBiQ92688.
TreeFamiTF317206.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92688-1) [UniParc]FASTAAdd to basket
Also known as: Anp32b1, PHAPI2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDMKRRIHLE LRNRTPAAVR ELVLDNCKSN DGKIEGLTAE FVNLEFLSLI
60 70 80 90 100
NVGLISVSNL PKLPKLKKLE LSENRIFGGL DMLAEKLPNL THLNLSGNKL
110 120 130 140 150
KDISTLEPLK KLECLKSLDL FNCEVTNLND YRESVFKLLP QLTYLDGYDR
160 170 180 190 200
EDQEAPDSDA EVDGVDEEEE DEEGEDEEDE DDEDGEEEEF DEEDDEDEDV
210 220 230 240 250
EGDEDDDEVS EEEEEFGLDE EDEDEDEDEE EEEGGKGEKR KRETDDEGED

D
Length:251
Mass (Da):28,788
Last modified:February 1, 1997 - v1
Checksum:i93A1AADF8EDE7D53
GO
Isoform 2 (identifier: Q92688-2) [UniParc]FASTAAdd to basket
Also known as: Anp32b2, PHAPI2b

The sequence of this isoform differs from the canonical sequence as follows:
     196-251: Missing.

Note: No canonical donor splice site.
Show »
Length:195
Mass (Da):22,277
Checksum:iC504A0E604F16F73
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 2Missing in CAA69265 (PubMed:9473664).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019304196 – 251Missing in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07569 mRNA. Translation: CAA68855.1.
U70439 mRNA. Translation: AAB37579.1.
Y07969 mRNA. Translation: CAA69265.1.
Y07570 mRNA. Translation: CAA68856.1.
AK313733 mRNA. Translation: BAG36474.1.
AL354726 Genomic DNA. Translation: CAI15260.1.
CH471105 Genomic DNA. Translation: EAW58865.1.
BC013003 mRNA. Translation: AAH13003.1.
BC019658 mRNA. Translation: AAH19658.1.
CCDSiCCDS6732.1. [Q92688-1]
RefSeqiNP_006392.1. NM_006401.2. [Q92688-1]
UniGeneiHs.730654.

Genome annotation databases

EnsembliENST00000339399; ENSP00000345848; ENSG00000136938. [Q92688-1]
GeneIDi10541.
KEGGihsa:10541.
UCSCiuc004aya.4. human. [Q92688-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07569 mRNA. Translation: CAA68855.1.
U70439 mRNA. Translation: AAB37579.1.
Y07969 mRNA. Translation: CAA69265.1.
Y07570 mRNA. Translation: CAA68856.1.
AK313733 mRNA. Translation: BAG36474.1.
AL354726 Genomic DNA. Translation: CAI15260.1.
CH471105 Genomic DNA. Translation: EAW58865.1.
BC013003 mRNA. Translation: AAH13003.1.
BC019658 mRNA. Translation: AAH19658.1.
CCDSiCCDS6732.1. [Q92688-1]
RefSeqiNP_006392.1. NM_006401.2. [Q92688-1]
UniGeneiHs.730654.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ELLNMR-A1-161[»]
2RR6NMR-A1-161[»]
ProteinModelPortaliQ92688.
SMRiQ92688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115795. 54 interactors.
IntActiQ92688. 34 interactors.
STRINGi9606.ENSP00000345848.

PTM databases

iPTMnetiQ92688.
PhosphoSitePlusiQ92688.
SwissPalmiQ92688.

Polymorphism and mutation databases

BioMutaiANP32B.
DMDMi26390818.

Proteomic databases

EPDiQ92688.
MaxQBiQ92688.
PaxDbiQ92688.
PeptideAtlasiQ92688.
PRIDEiQ92688.
TopDownProteomicsiQ92688-1. [Q92688-1]
Q92688-2. [Q92688-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339399; ENSP00000345848; ENSG00000136938. [Q92688-1]
GeneIDi10541.
KEGGihsa:10541.
UCSCiuc004aya.4. human. [Q92688-1]

Organism-specific databases

CTDi10541.
DisGeNETi10541.
GeneCardsiANP32B.
HGNCiHGNC:16677. ANP32B.
HPAiHPA063133.
HPA067561.
neXtProtiNX_Q92688.
OpenTargetsiENSG00000136938.
PharmGKBiPA24812.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiQ92688.
KOiK18647.
OMAiLINVGLM.
OrthoDBiEOG091G0R8Z.
PhylomeDBiQ92688.
TreeFamiTF317206.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136938-MONOMER.
SIGNORiQ92688.

Miscellaneous databases

ChiTaRSiANP32B. human.
EvolutionaryTraceiQ92688.
GeneWikiiANP32B.
GenomeRNAii10541.
PMAP-CutDBQ92688.
PROiQ92688.

Gene expression databases

BgeeiENSG00000136938.
CleanExiHS_ANP32B.
GenevisibleiQ92688. HS.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAN32B_HUMAN
AccessioniPrimary (citable) accession number: Q92688
Secondary accession number(s): B2R9C7
, O00655, P78458, P78459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.