Q92674 (CENPI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein I
Short name=CENP-I

Alternative name(s):
FSH primary response protein 1
Follicle-stimulating hormone primary response protein
Interphase centromere complex protein 19
Leucine-rich primary response protein 1

Gene names

Name:	CENPI
Synonyms:	FSHPRH1, ICEN19, LRPR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone. Ref.4 Ref.6
Subunit structure	Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts with SENP6. Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus. Chromosome › centromere. Note: Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. Ref.4 Ref.6
Induction	By follicle-stimulating hormone (FSH).
Post-translational modification	Sumoylated. Sumoylated form can be polyubiquitinated by RNF4, leading to its degradation. Desumoylation by SENP6 prevents its degradation. Ref.8
Sequence similarities	Belongs to the mis6 family.

Ontologies

Keywords
Cellular component	Centromere Chromosome Nucleus
Coding sequence diversity	Alternative splicing
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CENP-A containing nucleosome assembly at centromere Traceable author statement. Source: Reactome mitotic prometaphase Traceable author statement. Source: Reactome sex differentiation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytosol Traceable author statement. Source: Reactome kinetochore Inferred from direct assay Ref.8. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92674-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92674-2)

The sequence of this isoform differs from the canonical sequence as follows:
     523-756: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	Centromere protein I		PRO_0000087354
Natural variations
	Alternative sequence	523 – 756	234	Missing in isoform 2.		VSP_015797
Experimental info
	Sequence conflict	262 – 263	2	FK → LQ in CAA65884. Ref.1
	Sequence conflict	389	1	Y → H in CAA65884. Ref.1
	Sequence conflict	432	1	F → Y in CAA65884. Ref.1
	Sequence conflict	450	1	C → S in CAA65884. Ref.1
	Sequence conflict	532	1	S → C in CAA65884. Ref.1
	Sequence conflict	576	1	N → D in CAA65884. Ref.1
	Sequence conflict	642	1	H → Y in CAA65884. Ref.1
	Sequence conflict	658	1	G → A in CAA65884. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 2. Checksum: A3439CF1729D7A66	FASTA	756	86,720
	10 20 30 40 50 60 MSPQKRVKNV QAQNRTSQGS SSFQTTLSAW KVKQDPSNSK NISKHGQNNP VGDYEHADDQ 70 80 90 100 110 120 AEEDALQMAV GYFEKGPIKA SQNKDKTLEK HLKTVENVAW KNGLASEEID ILLNIALSGK 130 140 150 160 170 180 FGNAVNTRIL KCMIPATVIS EDSVVKAVSW LCVGKCSGST KVLFYRWLVA MFDFIDRKEQ 190 200 210 220 230 240 INLLYGFFFA SLQDDALCPY VCHLLYLLTK KENVKPFRVR KLLDLQAKMG MQPHLQALLS 250 260 270 280 290 300 LYKFFAPALI SVSLPVRKKI YFKNSENLWK TALLAVKQRN RGPSPEPLKL MLGPANVRPL 310 320 330 340 350 360 KRKWNSLSVI PVLNSSSYTK ECGKKEMSLS DCLNRSGSFP LEQLQSFPQL LQNIHCLELP 370 380 390 400 410 420 SQMGSVLNNS LLLHYINCVR DEPVLLRFYY WLSQTLQEEC IWYKVNNYEH GKEFTNFLDT 430 440 450 460 470 480 IIRAECFLQE GFYSCEAFLY KSLPLWDGLC CRSQFLQLVS WIPFSSFSEV KPLLFDHLAQ 490 500 510 520 530 540 LFFTSTIYFK CSVLQSLKEL LQNWLLWLSM DIHMKPVTNS PLETTLGGSM NSVSKLIHYV 550 560 570 580 590 600 GWLSTTAMRL ESNNTFLLHF ILDFYEKVCD IYINYNLPLV VLFPPGIFYS ALLSLDTSIL 610 620 630 640 650 660 NQLCFIMHRY RKNLTAAKKN ELVQKTKSEF NFSSKTYQEF NHYLTSMVGC LWTSKPFGKG 670 680 690 700 710 720 IYIDPEILEK TGVAEYKNSL NVVHHPSFLS YAVSFLLQES PEERTVNVSS IRGKKWSWYL 730 740 750 DYLFSQGLQG LKLFIRSSVH HSSIPRAEGI NCNNQY
	Isoform 2 [UniParc]. Checksum: BAB3AA874485BD51	FASTA	522	59,813
	10 20 30 40 50 60 MSPQKRVKNV QAQNRTSQGS SSFQTTLSAW KVKQDPSNSK NISKHGQNNP VGDYEHADDQ 70 80 90 100 110 120 AEEDALQMAV GYFEKGPIKA SQNKDKTLEK HLKTVENVAW KNGLASEEID ILLNIALSGK 130 140 150 160 170 180 FGNAVNTRIL KCMIPATVIS EDSVVKAVSW LCVGKCSGST KVLFYRWLVA MFDFIDRKEQ 190 200 210 220 230 240 INLLYGFFFA SLQDDALCPY VCHLLYLLTK KENVKPFRVR KLLDLQAKMG MQPHLQALLS 250 260 270 280 290 300 LYKFFAPALI SVSLPVRKKI YFKNSENLWK TALLAVKQRN RGPSPEPLKL MLGPANVRPL 310 320 330 340 350 360 KRKWNSLSVI PVLNSSSYTK ECGKKEMSLS DCLNRSGSFP LEQLQSFPQL LQNIHCLELP 370 380 390 400 410 420 SQMGSVLNNS LLLHYINCVR DEPVLLRFYY WLSQTLQEEC IWYKVNNYEH GKEFTNFLDT 430 440 450 460 470 480 IIRAECFLQE GFYSCEAFLY KSLPLWDGLC CRSQFLQLVS WIPFSSFSEV KPLLFDHLAQ 490 500 510 520 LFFTSTIYFK CSVLQSLKEL LQNWLLWLSM DIHMKPVTNS PL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and chromosomal location of a human homologue of LRPR1, an FSH primary response gene." Roberts R.G., Kendall E., Vetrie D., Bobrow M. Genomics 37:122-124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	"Human CENP-I specifies localization of CENP-F, MAD1 and MAD2 to kinetochores and is essential for mitosis." Liu S.-T., Hittle J.C., Jablonski S.A., Campbell M.S., Yoda K., Yen T.J. Nat. Cell Biol. 5:341-345(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells." Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K. Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres." Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T. Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPK; CENPN; CENPO; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
[7]	"The human CENP-A centromeric nucleosome-associated complex." Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W. Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPK; CENPL; CENPO; CENPP; CENPQ; CENPR AND CENPS.
[8]	"The SUMO protease SENP6 is essential for inner kinetochore assembly." Mukhopadhyay D., Arnaoutov A., Dasso M. J. Cell Biol. 188:681-692(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUMOYLATION, UBIQUITINATION BY RNF4, DESUMOYLATION BY SENP6, INTERACTION WITH SENP6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97249 mRNA. Translation: CAA65884.1. AL109963 Genomic DNA. Translation: CAB72340.1. AL109963 Genomic DNA. Translation: CAI42268.1. BC012462 mRNA. Translation: AAH12462.1.
IPI	IPI00552142. IPI00647405.
RefSeq	NP_006724.2. NM_006733.2.
UniGene	Hs.348920. Hs.737663.
3D structure databases
ProteinModelPortal	Q92674.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000218507.
PTM databases
PhosphoSite	Q92674.
Polymorphism databases
DMDM	77416860.
Proteomic databases
PaxDb	Q92674.
PRIDE	Q92674.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372926; ENSP00000362017; ENSG00000102384. ENST00000372927; ENSP00000362018; ENSG00000102384. ENST00000423383; ENSP00000399274; ENSG00000102384.
GeneID	2491.
KEGG	hsa:2491.
UCSC	uc004egx.3. human.
Organism-specific databases
CTD	2491.
GeneCards	GC0XP100353.
H-InvDB	HIX0016922.
HGNC	HGNC:3968. CENPI.
HPA	CAB012356.
MIM	300065. gene.
neXtProt	NX_Q92674.
PharmGKB	PA28385.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG16022.
HOGENOM	HOG000049199.
HOVERGEN	HBG080256.
InParanoid	Q92674.
KO	K11501.
OMA	KMGMQPH.
OrthoDB	EOG41RPTB.
PhylomeDB	Q92674.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
ArrayExpress	Q92674.
Bgee	Q92674.
CleanEx	HS_CENPI.
Genevestigator	Q92674.
GermOnline	ENSG00000102384. Homo sapiens.
Family and domain databases
InterPro	IPR012485. Centromere_CenpI. [Graphical view]
PANTHER	PTHR15408. PTHR15408. 1 hit.
Pfam	PF07778. CENP-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	2491.
NextBio	9839.
SOURCE	Search...

Entry information

Entry name

CENPI_HUMAN

Accession

Primary (citable) accession number: Q92674
Secondary accession number(s): Q5JWZ9, Q96ED0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 11, 2005
Last modified:	April 3, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.