Q92664 (TF3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor IIIA
Short name=TFIIIA

Gene names

Name:

GTF3A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Contains 9 C2H2-type zinc fingers.
Caution	It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.
Sequence caution	The sequence BAA06988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA06988.1 differs from that shown. Reason: Frameshift at positions 319 and 330.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gene expression Traceable author statement. Source: Reactome rRNA transcription Traceable author statement Ref.4. Source: ProtInc regulation of transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase III promoter Traceable author statement. Source: Reactome
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome nucleus Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92664-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92664-2)

The sequence of this isoform differs from the canonical sequence as follows:
     164-188: Missing.
     189-190: YV → LC

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Transcription factor IIIA		PRO_0000047080
Regions
	Zinc finger	40 – 64	25	C2H2-type 1
	Zinc finger	70 – 94	25	C2H2-type 2
	Zinc finger	100 – 125	26	C2H2-type 3
	Zinc finger	132 – 154	23	C2H2-type 4; atypical
	Zinc finger	162 – 186	25	C2H2-type 5
	Zinc finger	189 – 213	25	C2H2-type 6
	Zinc finger	217 – 239	23	C2H2-type 7
	Zinc finger	246 – 271	26	C2H2-type 8
	Zinc finger	277 – 301	25	C2H2-type 9
Natural variations
	Alternative sequence	164 – 188	25	Missing in isoform 2.		VSP_031525
	Alternative sequence	189 – 190	2	YV → LC in isoform 2.		VSP_031526
	Natural variant	245	1	V → L. Corresponds to variant rs7323 [ dbSNP | Ensembl ].		VAR_014824
Experimental info
	Sequence conflict	156	1	H → N in BAA06988. Ref.1
	Sequence conflict	319	1	R → G in BAA06988. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 261EBDCC550130B9	FASTA	365	41,515
	10 20 30 40 50 60 MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH 70 80 90 100 110 120 LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF VCAANGCDQK FNTKSNLKKH 130 140 150 160 170 180 FERKHENQQK QYICSFEDCK KTFKKHQQLK IHQCQHTNEP LFKCTQEGCG KHFASPSKLK 190 200 210 220 230 240 RHAKAHEGYV CQKGCSFVAK TWTELLKHVR ETHKEEILCE VCRKTFKRKD YLKQHMKTHA 250 260 270 280 290 300 PERDVCRCPR EGCGRTYTTV FNLQSHILSF HEESRPFVCE HAGCGKTFAM KQSLTRHAVV 310 320 330 340 350 360 HDPDKKKMKL KVKKSREKRS LASHLSGYIP PKRKQGQGLS LCQNGESPNC VEDKMLSTVA VLTLG
	Isoform 2 [UniParc]. Checksum: EAECD6B619A6BC7C	FASTA	340	38,779
	10 20 30 40 50 60 MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH 70 80 90 100 110 120 LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF VCAANGCDQK FNTKSNLKKH 130 140 150 160 170 180 FERKHENQQK QYICSFEDCK KTFKKHQQLK IHQCQHTNEP LFKLCCQKGC SFVAKTWTEL 190 200 210 220 230 240 LKHVRETHKE EILCEVCRKT FKRKDYLKQH MKTHAPERDV CRCPREGCGR TYTTVFNLQS 250 260 270 280 290 300 HILSFHEESR PFVCEHAGCG KTFAMKQSLT RHAVVHDPDK KKMKLKVKKS REKRSLASHL 310 320 330 340 SGYIPPKRKQ GQGLSLCQNG ESPNCVEDKM LSTVAVLTLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA." Arakawa H., Nagase H., Hayashi N., Ogawa M., Nagata M., Fujiwara T., Takahashi E., Shin S., Nakamura Y. Cytogenet. Cell Genet. 70:235-238(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA." Drew P.D., Nagle J.W., Canning R.D., Ozato K., Biddison W.E., Becker K.G. Gene 159:215-218(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-365 (ISOFORMS 1 AND 2). Tissue: Brain.
[4]	"Purification and characterization of human transcription factor IIIA." Moorefield B., Roeder R.G. J. Biol. Chem. 269:20857-20865(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D32257 mRNA. Translation: BAA06988.1. Sequence problems. AL137059 Genomic DNA. Translation: CAX15101.1. U20272 mRNA. Translation: AAA75623.1. U14134 mRNA. Translation: AAA21873.1.
PIR	I38937.
RefSeq	NP_002088.2. NM_002097.2.
UniGene	Hs.445977.
3D structure databases
ProteinModelPortal	Q92664.
SMR	Q92664. Positions 34-350.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	109226. 6 interactions.
IntAct	Q92664. 6 interactions.
STRING	9606.ENSP00000370532.
PTM databases
PhosphoSite	Q92664.
Polymorphism databases
DMDM	172045838.
Proteomic databases
PaxDb	Q92664.
PRIDE	Q92664.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381140; ENSP00000370532; ENSG00000122034.
GeneID	2971.
KEGG	hsa:2971.
UCSC	uc001ure.2. human. [Q92664-1]
Organism-specific databases
CTD	2971.
GeneCards	GC13P027998.
H-InvDB	HIX0011192. HIX0130586.
HGNC	HGNC:4662. GTF3A.
HPA	HPA007990.
MIM	600860. gene.
neXtProt	NX_Q92664.
PharmGKB	PA29048.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000232112.
HOVERGEN	HBG017915.
InParanoid	Q92664.
KO	K09191.
PhylomeDB	Q92664.
TreeFam	TF333011.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q92664.
Bgee	Q92664.
CleanEx	HS_GTF3A.
Genevestigator	Q92664.
Family and domain databases
Gene3D	3.30.160.60. 8 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00096. zf-C2H2. 4 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 9 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 8 hits. PS50157. ZINC_FINGER_C2H2_2. 8 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GTF3A. human.
GeneWiki	GTF3A.
GenomeRNAi	2971.
NextBio	11783.
PRO	Q92664.
SOURCE	Search...

Entry information

Entry name

TF3A_HUMAN

Accession

Primary (citable) accession number: Q92664
Secondary accession number(s): B7ZBK5, Q12963, Q13097

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 26, 2008
Last modified:	April 16, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.