Reviewed, UniProtKB/Swiss-Prot Q92664 (TF3A_HUMAN)

Names and origin

Protein names

Recommended name:
    Transcription factor IIIA
      Short name=Factor A
      Short name=TFIIIA

Gene names

Name:

GTF3A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Contains 9 C2H2-type zinc fingers.
Caution	It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.
Sequence caution	The sequence BAA06988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA06988.1 differs from that shown. Reason: Frameshift at positions 319 and 330.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding RNA-binding Zinc
Gene Ontology (GO)
Biological process	rRNA transcription Ref.3 Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase III promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase III transcription factor activity Ref.3 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABL1	P00519	1	EBI-1752104,EBI-375543
FYN	P06241	1	EBI-1752104,EBI-515315

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q92664-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q92664-2)

The sequence of this isoform differs from the canonical sequence as follows:
     164-188: Missing.
     189-190: YV → LC

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Transcription factor IIIA		PRO_0000047080
Regions
	Zinc finger	40 – 64	25	C2H2-type 1
	Zinc finger	70 – 94	25	C2H2-type 2
	Zinc finger	100 – 125	26	C2H2-type 3
	Zinc finger	132 – 154	23	C2H2-type 4; atypical
	Zinc finger	162 – 186	25	C2H2-type 5
	Zinc finger	189 – 213	25	C2H2-type 6
	Zinc finger	217 – 239	23	C2H2-type 7
	Zinc finger	246 – 271	26	C2H2-type 8
	Zinc finger	277 – 301	25	C2H2-type 9
Natural variations
	Alternative sequence	164 – 188	25	Missing in isoform 2.		VSP_031525
	Alternative sequence	189 – 190	2	YV → LC in isoform 2.		VSP_031526
	Natural variant	245	1	V → L: dbSNP rs7323.		VAR_014824
Experimental info
	Sequence conflict	156	1	H → N in BAA06988. Ref.1
	Sequence conflict	319	1	R → G in BAA06988. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 261EBDCC550130B9	FASTA	365	41,515
	10 20 30 40 50 60 MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH 70 80 90 100 110 120 LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF VCAANGCDQK FNTKSNLKKH 130 140 150 160 170 180 FERKHENQQK QYICSFEDCK KTFKKHQQLK IHQCQHTNEP LFKCTQEGCG KHFASPSKLK 190 200 210 220 230 240 RHAKAHEGYV CQKGCSFVAK TWTELLKHVR ETHKEEILCE VCRKTFKRKD YLKQHMKTHA 250 260 270 280 290 300 PERDVCRCPR EGCGRTYTTV FNLQSHILSF HEESRPFVCE HAGCGKTFAM KQSLTRHAVV 310 320 330 340 350 360 HDPDKKKMKL KVKKSREKRS LASHLSGYIP PKRKQGQGLS LCQNGESPNC VEDKMLSTVA VLTLG
	Isoform 2 [UniParc]. Checksum: EAECD6B619A6BC7C		340	38,779
	10 20 30 40 50 60 MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH 70 80 90 100 110 120 LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF VCAANGCDQK FNTKSNLKKH 130 140 150 160 170 180 FERKHENQQK QYICSFEDCK KTFKKHQQLK IHQCQHTNEP LFKLCCQKGC SFVAKTWTEL 190 200 210 220 230 240 LKHVRETHKE EILCEVCRKT FKRKDYLKQH MKTHAPERDV CRCPREGCGR TYTTVFNLQS 250 260 270 280 290 300 HILSFHEESR PFVCEHAGCG KTFAMKQSLT RHAVVHDPDK KKMKLKVKKS REKRSLASHL 310 320 330 340 SGYIPPKRKQ GQGLSLCQNG ESPNCVEDKM LSTVAVLTLG

References

[1]	"Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA." Arakawa H., Nagase H., Hayashi N., Ogawa M., Nagata M., Fujiwara T., Takahashi E., Shin S., Nakamura Y. Cytogenet. Cell Genet. 70:235-238(1995) [PubMed: 7789179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA." Drew P.D., Nagle J.W., Canning R.D., Ozato K., Biddison W.E., Becker K.G. Gene 159:215-218(1995) [PubMed: 7622052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-365 (ISOFORMS 1 AND 2). Tissue: Brain.
[3]	"Purification and characterization of human transcription factor IIIA." Moorefield B., Roeder R.G. J. Biol. Chem. 269:20857-20865(1994) [PubMed: 8063702] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D32257 mRNA. Translation: BAA06988.1. Sequence problems. U20272 mRNA. Translation: AAA75623.1. U14134 mRNA. Translation: AAA21873.1.
PIR	I38937.
RefSeq	NP_002088.2.
UniGene	Hs.445977
3D structure databases
HSSP	HSSP built from PDB template 1TF3 based on UniProtKB P03001.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q92664.
Genome annotation databases
Ensembl	ENSG00000122034. Homo sapiens. [Contig view]
GeneID	2971.
KEGG	hsa:2971.
Organism-specific databases
H-InvDB	HIX0011192. HIX0059188.
HGNC	HGNC:4662. GTF3A.
MIM	600860. gene.
PharmGKB	PA29048.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q92664.
HOVERGEN	Q92664.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
CleanEx	HS_GTF3A.
GermOnline	ENSG00000122034. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 6 hits.
Pfam	PF00096. zf-C2H2. 8 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00355. ZnF_C2H2. 9 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 8 hits. PS50157. ZINC_FINGER_C2H2_2. 8 hits. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q92664.
NextBio	11783.
SOURCE	Search...

Entry information

Entry name

TF3A_HUMAN

Accession

Primary (citable) accession number: Q92664
Secondary accession number(s): Q12963, Q13097

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 26, 2008
Last modified:	November 25, 2008
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)