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Q92664

- TF3A_HUMAN

UniProt

Q92664 - TF3A_HUMAN

Protein

Transcription factor IIIA

Gene

GTF3A

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri40 – 6425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri70 – 9425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri100 – 12526C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri132 – 15423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri162 – 18625C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri189 – 21325C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri217 – 23923C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri246 – 27126C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri277 – 30125C2H2-type 9PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. gene expression Source: Reactome
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. rRNA transcription Source: ProtInc
    4. transcription from RNA polymerase III promoter Source: Reactome

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor IIIA
    Short name:
    TFIIIA
    Gene namesi
    Name:GTF3A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:4662. GTF3A.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: Reactome
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29048.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Transcription factor IIIAPRO_0000047080Add
    BLAST

    Proteomic databases

    MaxQBiQ92664.
    PaxDbiQ92664.
    PRIDEiQ92664.

    PTM databases

    PhosphoSiteiQ92664.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiQ92664.
    BgeeiQ92664.
    CleanExiHS_GTF3A.
    GenevestigatoriQ92664.

    Organism-specific databases

    HPAiHPA007990.

    Interactioni

    Protein-protein interaction databases

    BioGridi109226. 6 interactions.
    IntActiQ92664. 6 interactions.
    STRINGi9606.ENSP00000370532.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92664.
    SMRiQ92664. Positions 38-330.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri40 – 6425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri70 – 9425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri100 – 12526C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri132 – 15423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri162 – 18625C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri189 – 21325C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri217 – 23923C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri246 – 27126C2H2-type 8PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri277 – 30125C2H2-type 9PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000232112.
    HOVERGENiHBG017915.
    InParanoidiQ92664.
    KOiK09191.
    PhylomeDBiQ92664.
    TreeFamiTF333011.

    Family and domain databases

    Gene3Di3.30.160.60. 8 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 4 hits.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 9 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
    PS50157. ZINC_FINGER_C2H2_2. 8 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q92664-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN    50
    YSKAWKLDAH LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF 100
    VCAANGCDQK FNTKSNLKKH FERKHENQQK QYICSFEDCK KTFKKHQQLK 150
    IHQCQHTNEP LFKCTQEGCG KHFASPSKLK RHAKAHEGYV CQKGCSFVAK 200
    TWTELLKHVR ETHKEEILCE VCRKTFKRKD YLKQHMKTHA PERDVCRCPR 250
    EGCGRTYTTV FNLQSHILSF HEESRPFVCE HAGCGKTFAM KQSLTRHAVV 300
    HDPDKKKMKL KVKKSREKRS LASHLSGYIP PKRKQGQGLS LCQNGESPNC 350
    VEDKMLSTVA VLTLG 365
    Length:365
    Mass (Da):41,515
    Last modified:February 26, 2008 - v3
    Checksum:i261EBDCC550130B9
    GO
    Isoform 2 (identifier: Q92664-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         164-188: Missing.
         189-190: YV → LC

    Show »
    Length:340
    Mass (Da):38,779
    Checksum:iEAECD6B619A6BC7C
    GO

    Sequence cautioni

    The sequence BAA06988.1 differs from that shown. Reason: Frameshift at positions 319 and 330.
    The sequence BAA06988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1561H → N in BAA06988. (PubMed:7789179)Curated
    Sequence conflicti319 – 3191R → G in BAA06988. (PubMed:7789179)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti245 – 2451V → L.
    Corresponds to variant rs7323 [ dbSNP | Ensembl ].
    VAR_014824

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei164 – 18825Missing in isoform 2. 1 PublicationVSP_031525Add
    BLAST
    Alternative sequencei189 – 1902YV → LC in isoform 2. 1 PublicationVSP_031526

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32257 mRNA. Translation: BAA06988.1. Sequence problems.
    AL137059 Genomic DNA. Translation: CAX15101.1.
    U20272 mRNA. Translation: AAA75623.1.
    U14134 mRNA. Translation: AAA21873.1.
    CCDSiCCDS45019.1. [Q92664-1]
    PIRiI38937.
    RefSeqiNP_002088.2. NM_002097.2. [Q92664-1]
    UniGeneiHs.445977.

    Genome annotation databases

    EnsembliENST00000381140; ENSP00000370532; ENSG00000122034.
    GeneIDi2971.
    KEGGihsa:2971.
    UCSCiuc001ure.2. human. [Q92664-1]

    Polymorphism databases

    DMDMi172045838.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32257 mRNA. Translation: BAA06988.1 . Sequence problems.
    AL137059 Genomic DNA. Translation: CAX15101.1 .
    U20272 mRNA. Translation: AAA75623.1 .
    U14134 mRNA. Translation: AAA21873.1 .
    CCDSi CCDS45019.1. [Q92664-1 ]
    PIRi I38937.
    RefSeqi NP_002088.2. NM_002097.2. [Q92664-1 ]
    UniGenei Hs.445977.

    3D structure databases

    ProteinModelPortali Q92664.
    SMRi Q92664. Positions 38-330.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109226. 6 interactions.
    IntActi Q92664. 6 interactions.
    STRINGi 9606.ENSP00000370532.

    PTM databases

    PhosphoSitei Q92664.

    Polymorphism databases

    DMDMi 172045838.

    Proteomic databases

    MaxQBi Q92664.
    PaxDbi Q92664.
    PRIDEi Q92664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000381140 ; ENSP00000370532 ; ENSG00000122034 .
    GeneIDi 2971.
    KEGGi hsa:2971.
    UCSCi uc001ure.2. human. [Q92664-1 ]

    Organism-specific databases

    CTDi 2971.
    GeneCardsi GC13P027998.
    H-InvDB HIX0011192.
    HIX0130586.
    HGNCi HGNC:4662. GTF3A.
    HPAi HPA007990.
    MIMi 600860. gene.
    neXtProti NX_Q92664.
    PharmGKBi PA29048.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000232112.
    HOVERGENi HBG017915.
    InParanoidi Q92664.
    KOi K09191.
    PhylomeDBi Q92664.
    TreeFami TF333011.

    Enzyme and pathway databases

    Reactomei REACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
    REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

    Miscellaneous databases

    ChiTaRSi GTF3A. human.
    GeneWikii GTF3A.
    GenomeRNAii 2971.
    NextBioi 11783.
    PROi Q92664.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q92664.
    Bgeei Q92664.
    CleanExi HS_GTF3A.
    Genevestigatori Q92664.

    Family and domain databases

    Gene3Di 3.30.160.60. 8 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 4 hits.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 9 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 8 hits.
    PS50157. ZINC_FINGER_C2H2_2. 8 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA."
      Arakawa H., Nagase H., Hayashi N., Ogawa M., Nagata M., Fujiwara T., Takahashi E., Shin S., Nakamura Y.
      Cytogenet. Cell Genet. 70:235-238(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Fetal brain.
    2. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA."
      Drew P.D., Nagle J.W., Canning R.D., Ozato K., Biddison W.E., Becker K.G.
      Gene 159:215-218(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-365 (ISOFORMS 1 AND 2).
      Tissue: Brain.
    4. "Purification and characterization of human transcription factor IIIA."
      Moorefield B., Roeder R.G.
      J. Biol. Chem. 269:20857-20865(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTF3A_HUMAN
    AccessioniPrimary (citable) accession number: Q92664
    Secondary accession number(s): B7ZBK5, Q12963, Q13097
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3