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Q92664 (TF3A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor IIIA

Short name=TFIIIA
Gene names
Name:GTF3A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.

Subcellular location

Nucleus.

Tissue specificity

Ubiquitous.

Sequence similarities

Contains 9 C2H2-type zinc fingers.

Caution

It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.

Sequence caution

The sequence BAA06988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAA06988.1 differs from that shown. Reason: Frameshift at positions 319 and 330.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q92664-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q92664-2)

The sequence of this isoform differs from the canonical sequence as follows:
     164-188: Missing.
     189-190: YV → LC

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Transcription factor IIIA
PRO_0000047080

Regions

Zinc finger40 – 6425C2H2-type 1
Zinc finger70 – 9425C2H2-type 2
Zinc finger100 – 12526C2H2-type 3
Zinc finger132 – 15423C2H2-type 4; atypical
Zinc finger162 – 18625C2H2-type 5
Zinc finger189 – 21325C2H2-type 6
Zinc finger217 – 23923C2H2-type 7
Zinc finger246 – 27126C2H2-type 8
Zinc finger277 – 30125C2H2-type 9

Natural variations

Alternative sequence164 – 18825Missing in isoform 2.
VSP_031525
Alternative sequence189 – 1902YV → LC in isoform 2.
VSP_031526
Natural variant2451V → L.
Corresponds to variant rs7323 [ dbSNP | Ensembl ].
VAR_014824

Experimental info

Sequence conflict1561H → N in BAA06988. Ref.1
Sequence conflict3191R → G in BAA06988. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 26, 2008. Version 3.
Checksum: 261EBDCC550130B9

FASTA36541,515
        10         20         30         40         50         60 
MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN YSKAWKLDAH 

        70         80         90        100        110        120 
LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF VCAANGCDQK FNTKSNLKKH 

       130        140        150        160        170        180 
FERKHENQQK QYICSFEDCK KTFKKHQQLK IHQCQHTNEP LFKCTQEGCG KHFASPSKLK 

       190        200        210        220        230        240 
RHAKAHEGYV CQKGCSFVAK TWTELLKHVR ETHKEEILCE VCRKTFKRKD YLKQHMKTHA 

       250        260        270        280        290        300 
PERDVCRCPR EGCGRTYTTV FNLQSHILSF HEESRPFVCE HAGCGKTFAM KQSLTRHAVV 

       310        320        330        340        350        360 
HDPDKKKMKL KVKKSREKRS LASHLSGYIP PKRKQGQGLS LCQNGESPNC VEDKMLSTVA 


VLTLG 

« Hide

Isoform 2 [UniParc].

Checksum: EAECD6B619A6BC7C
Show »

FASTA34038,779

References

« Hide 'large scale' references
[1]"Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA."
Arakawa H., Nagase H., Hayashi N., Ogawa M., Nagata M., Fujiwara T., Takahashi E., Shin S., Nakamura Y.
Cytogenet. Cell Genet. 70:235-238(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Fetal brain.
[2]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA."
Drew P.D., Nagle J.W., Canning R.D., Ozato K., Biddison W.E., Becker K.G.
Gene 159:215-218(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-365 (ISOFORMS 1 AND 2).
Tissue: Brain.
[4]"Purification and characterization of human transcription factor IIIA."
Moorefield B., Roeder R.G.
J. Biol. Chem. 269:20857-20865(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D32257 mRNA. Translation: BAA06988.1. Sequence problems.
AL137059 Genomic DNA. Translation: CAX15101.1.
U20272 mRNA. Translation: AAA75623.1.
U14134 mRNA. Translation: AAA21873.1.
CCDSCCDS45019.1. [Q92664-1]
PIRI38937.
RefSeqNP_002088.2. NM_002097.2. [Q92664-1]
UniGeneHs.445977.

3D structure databases

ProteinModelPortalQ92664.
SMRQ92664. Positions 38-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109226. 6 interactions.
IntActQ92664. 6 interactions.
STRING9606.ENSP00000370532.

PTM databases

PhosphoSiteQ92664.

Polymorphism databases

DMDM172045838.

Proteomic databases

MaxQBQ92664.
PaxDbQ92664.
PRIDEQ92664.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000381140; ENSP00000370532; ENSG00000122034.
GeneID2971.
KEGGhsa:2971.
UCSCuc001ure.2. human. [Q92664-1]

Organism-specific databases

CTD2971.
GeneCardsGC13P027998.
H-InvDBHIX0011192.
HIX0130586.
HGNCHGNC:4662. GTF3A.
HPAHPA007990.
MIM600860. gene.
neXtProtNX_Q92664.
PharmGKBPA29048.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000232112.
HOVERGENHBG017915.
InParanoidQ92664.
KOK09191.
PhylomeDBQ92664.
TreeFamTF333011.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ92664.
BgeeQ92664.
CleanExHS_GTF3A.
GenevestigatorQ92664.

Family and domain databases

Gene3D3.30.160.60. 8 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGTF3A. human.
GeneWikiGTF3A.
GenomeRNAi2971.
NextBio11783.
PROQ92664.
SOURCESearch...

Entry information

Entry nameTF3A_HUMAN
AccessionPrimary (citable) accession number: Q92664
Secondary accession number(s): B7ZBK5, Q12963, Q13097
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: July 9, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM