Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor IIIA

Gene

GTF3A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri40 – 6425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri70 – 9425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri100 – 12526C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 18625C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri189 – 21325C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri217 – 23923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 27126C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri277 – 30125C2H2-type 9PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIA
Short name:
TFIIIA
Gene namesi
Name:GTF3A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:4662. GTF3A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29048.

Polymorphism and mutation databases

BioMutaiGTF3A.
DMDMi172045838.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Transcription factor IIIAPRO_0000047080Add
BLAST

Proteomic databases

MaxQBiQ92664.
PaxDbiQ92664.
PRIDEiQ92664.

PTM databases

PhosphoSiteiQ92664.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiQ92664.
CleanExiHS_GTF3A.
ExpressionAtlasiQ92664. baseline and differential.
GenevestigatoriQ92664.

Organism-specific databases

HPAiHPA007990.

Interactioni

Protein-protein interaction databases

BioGridi109226. 5 interactions.
IntActiQ92664. 6 interactions.
STRINGi9606.ENSP00000370532.

Structurei

3D structure databases

ProteinModelPortaliQ92664.
SMRiQ92664. Positions 38-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri40 – 6425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri70 – 9425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri100 – 12526C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15423C2H2-type 4; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 18625C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri189 – 21325C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri217 – 23923C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri246 – 27126C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri277 – 30125C2H2-type 9PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000232112.
HOVERGENiHBG017915.
InParanoidiQ92664.
KOiK09191.
PhylomeDBiQ92664.
TreeFamiTF333011.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92664-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPPAVVAES VSSLTIADAF IAAGESSAPT PPRPALPRRF ICSFPDCSAN
60 70 80 90 100
YSKAWKLDAH LCKHTGERPF VCDYEGCGKA FIRDYHLSRH ILTHTGEKPF
110 120 130 140 150
VCAANGCDQK FNTKSNLKKH FERKHENQQK QYICSFEDCK KTFKKHQQLK
160 170 180 190 200
IHQCQHTNEP LFKCTQEGCG KHFASPSKLK RHAKAHEGYV CQKGCSFVAK
210 220 230 240 250
TWTELLKHVR ETHKEEILCE VCRKTFKRKD YLKQHMKTHA PERDVCRCPR
260 270 280 290 300
EGCGRTYTTV FNLQSHILSF HEESRPFVCE HAGCGKTFAM KQSLTRHAVV
310 320 330 340 350
HDPDKKKMKL KVKKSREKRS LASHLSGYIP PKRKQGQGLS LCQNGESPNC
360
VEDKMLSTVA VLTLG
Length:365
Mass (Da):41,515
Last modified:February 26, 2008 - v3
Checksum:i261EBDCC550130B9
GO
Isoform 2 (identifier: Q92664-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-188: Missing.
     189-190: YV → LC

Show »
Length:340
Mass (Da):38,779
Checksum:iEAECD6B619A6BC7C
GO

Sequence cautioni

The sequence BAA06988.1 differs from that shown. Reason: Frameshift at positions 319 and 330. Curated
The sequence BAA06988.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561H → N in BAA06988 (PubMed:7789179).Curated
Sequence conflicti319 – 3191R → G in BAA06988 (PubMed:7789179).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti245 – 2451V → L.
Corresponds to variant rs7323 [ dbSNP | Ensembl ].
VAR_014824

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei164 – 18825Missing in isoform 2. 1 PublicationVSP_031525Add
BLAST
Alternative sequencei189 – 1902YV → LC in isoform 2. 1 PublicationVSP_031526

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32257 mRNA. Translation: BAA06988.1. Sequence problems.
AL137059 Genomic DNA. Translation: CAX15101.1.
U20272 mRNA. Translation: AAA75623.1.
U14134 mRNA. Translation: AAA21873.1.
CCDSiCCDS45019.1. [Q92664-1]
PIRiI38937.
RefSeqiNP_002088.2. NM_002097.2. [Q92664-1]
UniGeneiHs.445977.

Genome annotation databases

EnsembliENST00000381140; ENSP00000370532; ENSG00000122034.
GeneIDi2971.
KEGGihsa:2971.
UCSCiuc001ure.2. human. [Q92664-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32257 mRNA. Translation: BAA06988.1. Sequence problems.
AL137059 Genomic DNA. Translation: CAX15101.1.
U20272 mRNA. Translation: AAA75623.1.
U14134 mRNA. Translation: AAA21873.1.
CCDSiCCDS45019.1. [Q92664-1]
PIRiI38937.
RefSeqiNP_002088.2. NM_002097.2. [Q92664-1]
UniGeneiHs.445977.

3D structure databases

ProteinModelPortaliQ92664.
SMRiQ92664. Positions 38-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109226. 5 interactions.
IntActiQ92664. 6 interactions.
STRINGi9606.ENSP00000370532.

PTM databases

PhosphoSiteiQ92664.

Polymorphism and mutation databases

BioMutaiGTF3A.
DMDMi172045838.

Proteomic databases

MaxQBiQ92664.
PaxDbiQ92664.
PRIDEiQ92664.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000381140; ENSP00000370532; ENSG00000122034.
GeneIDi2971.
KEGGihsa:2971.
UCSCiuc001ure.2. human. [Q92664-1]

Organism-specific databases

CTDi2971.
GeneCardsiGC13P027998.
H-InvDBHIX0011192.
HIX0130586.
HGNCiHGNC:4662. GTF3A.
HPAiHPA007990.
MIMi600860. gene.
neXtProtiNX_Q92664.
PharmGKBiPA29048.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000232112.
HOVERGENiHBG017915.
InParanoidiQ92664.
KOiK09191.
PhylomeDBiQ92664.
TreeFamiTF333011.

Enzyme and pathway databases

ReactomeiREACT_22339. RNA Polymerase III Abortive And Retractive Initiation.
REACT_347. RNA Polymerase III Transcription Initiation From Type 1 Promoter.

Miscellaneous databases

ChiTaRSiGTF3A. human.
GeneWikiiGTF3A.
GenomeRNAii2971.
NextBioi11783.
PROiQ92664.
SOURCEiSearch...

Gene expression databases

BgeeiQ92664.
CleanExiHS_GTF3A.
ExpressionAtlasiQ92664. baseline and differential.
GenevestigatoriQ92664.

Family and domain databases

Gene3Di3.30.160.60. 8 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 4 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA."
    Arakawa H., Nagase H., Hayashi N., Ogawa M., Nagata M., Fujiwara T., Takahashi E., Shin S., Nakamura Y.
    Cytogenet. Cell Genet. 70:235-238(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  2. "The DNA sequence and analysis of human chromosome 13."
    Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
    Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Cloning and expression analysis of a human cDNA homologous to Xenopus TFIIIA."
    Drew P.D., Nagle J.W., Canning R.D., Ozato K., Biddison W.E., Becker K.G.
    Gene 159:215-218(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-365 (ISOFORMS 1 AND 2).
    Tissue: Brain.
  4. "Purification and characterization of human transcription factor IIIA."
    Moorefield B., Roeder R.G.
    J. Biol. Chem. 269:20857-20865(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTF3A_HUMAN
AccessioniPrimary (citable) accession number: Q92664
Secondary accession number(s): B7ZBK5, Q12963, Q13097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 26, 2008
Last modified: May 27, 2015
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.