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Protein

GPI-anchor transamidase

Gene

PIGK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1641 Publication1
Active sitei2061 Publication1

GO - Molecular functioni

  • cysteine-type peptidase activity Source: UniProtKB-KW
  • GPI-anchor transamidase activity Source: UniProtKB
  • protein disulfide isomerase activity Source: UniProtKB

GO - Biological processi

  • attachment of GPI anchor to protein Source: UniProtKB
  • protein localization to cell surface Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142892-MONOMER.
ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.
UniPathwayiUPA00196.

Protein family/group databases

MEROPSiC13.005.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI-anchor transamidase (EC:3.-.-.-)
Short name:
GPI transamidase
Alternative name(s):
GPI8 homolog
Short name:
hGPI8
Phosphatidylinositol-glycan biosynthesis class K protein
Short name:
PIG-K
Gene namesi
Name:PIGK
Synonyms:GPI8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:8965. PIGK.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 367LumenalSequence analysisAdd BLAST340
Transmembranei368 – 388HelicalSequence analysisAdd BLAST21
Topological domaini389 – 395CytoplasmicSequence analysis7

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: Reactome
  • GPI-anchor transamidase complex Source: UniProtKB
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92C → A: Partial loss of activity. 2 Publications1
Mutagenesisi92C → S: Decrease in activity. 2 Publications1
Mutagenesisi164H → A: Loss of activity. 1 Publication1
Mutagenesisi206C → A: Loss of activity. 1 Publication1
Mutagenesisi311 – 395Missing : Loss of activity. 1 PublicationAdd BLAST85

Organism-specific databases

DisGeNETi10026.
OpenTargetsiENSG00000142892.
PharmGKBiPA33296.

Polymorphism and mutation databases

BioMutaiPIGK.
DMDMi22001630.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Combined sources1 PublicationAdd BLAST27
ChainiPRO_000002652928 – 395GPI-anchor transamidaseAdd BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi92Interchain (with C-182 in PIGT)1 Publication

Post-translational modificationi

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ92643.
MaxQBiQ92643.
PaxDbiQ92643.
PeptideAtlasiQ92643.
PRIDEiQ92643.

PTM databases

iPTMnetiQ92643.
PhosphoSitePlusiQ92643.

Expressioni

Gene expression databases

BgeeiENSG00000142892.
CleanExiHS_PIGK.
ExpressionAtlasiQ92643. baseline and differential.
GenevisibleiQ92643. HS.

Organism-specific databases

HPAiHPA056383.
HPA057040.

Interactioni

Subunit structurei

Forms a complex with PIGT, PIGS, PIGU and GAA1.2 Publications

Protein-protein interaction databases

BioGridi115343. 12 interactors.
IntActiQ92643. 4 interactors.
MINTiMINT-2813159.
STRINGi9606.ENSP00000359848.

Structurei

3D structure databases

ProteinModelPortaliQ92643.
SMRiQ92643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni311 – 321Essential for GPI attachmentAdd BLAST11

Sequence similaritiesi

Belongs to the peptidase C13 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1349. Eukaryota.
COG5206. LUCA.
GeneTreeiENSGT00530000063391.
HOGENOMiHOG000204398.
HOVERGENiHBG027488.
InParanoidiQ92643.
KOiK05290.
OMAiMACNHRN.
OrthoDBiEOG091G0BL9.
PhylomeDBiQ92643.
TreeFamiTF300848.

Family and domain databases

InterProiIPR028361. GPI_transamidase.
IPR001096. Peptidase_C13.
[Graphical view]
PANTHERiPTHR12000. PTHR12000. 1 hit.
PTHR12000:SF1. PTHR12000:SF1. 1 hit.
PfamiPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFiPIRSF500138. GPI8. 1 hit.
PIRSF019663. Legumain. 1 hit.
PRINTSiPR00776. HEMOGLOBNASE.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92643-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVTDSLSRA ATVLATVLLL SFGSVAASHI EDQAEQFFRS GHTNNWAVLV
60 70 80 90 100
CTSRFWFNYR HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA
110 120 130 140 150
TVFSHKNMEL NVYGDDVEVD YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL
160 170 180 190 200
SDDRSNILIY MTGHGGNGFL KFQDSEEITN IELADAFEQM WQKRRYNELL
210 220 230 240 250
FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA IGVHLMDRYT
260 270 280 290 300
FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI
310 320 330 340 350
TDFFGSVRKV EITTETIKLQ QDSEIMESSY KEDQMDEKLM EPLKYAEQLP
360 370 380 390
VAQIIHQKPK LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF
Length:395
Mass (Da):45,252
Last modified:July 11, 2002 - v2
Checksum:iAF706DDDAD13EFB2
GO
Isoform 2 (identifier: Q92643-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-125: Missing.

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):36,416
Checksum:i994A9F50C02CAEB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 4MAVT → SLHEA (PubMed:8978684).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05151816T → A.Corresponds to variant rs12723684dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05645750 – 125Missing in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07596 mRNA. Translation: CAA68871.1.
AF022913 mRNA. Translation: AAB81597.1.
AK304340 mRNA. Translation: BAG65185.1.
AK313015 mRNA. Translation: BAG35850.1.
AL035409, AC093433, AC113935 Genomic DNA. Translation: CAI21819.1.
CH471059 Genomic DNA. Translation: EAX06380.1.
BC020737 mRNA. Translation: AAH20737.1.
CCDSiCCDS674.1. [Q92643-1]
RefSeqiNP_005473.1. NM_005482.2. [Q92643-1]
UniGeneiHs.178305.

Genome annotation databases

EnsembliENST00000370812; ENSP00000359848; ENSG00000142892. [Q92643-1]
GeneIDi10026.
KEGGihsa:10026.
UCSCiuc001dhk.4. human. [Q92643-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07596 mRNA. Translation: CAA68871.1.
AF022913 mRNA. Translation: AAB81597.1.
AK304340 mRNA. Translation: BAG65185.1.
AK313015 mRNA. Translation: BAG35850.1.
AL035409, AC093433, AC113935 Genomic DNA. Translation: CAI21819.1.
CH471059 Genomic DNA. Translation: EAX06380.1.
BC020737 mRNA. Translation: AAH20737.1.
CCDSiCCDS674.1. [Q92643-1]
RefSeqiNP_005473.1. NM_005482.2. [Q92643-1]
UniGeneiHs.178305.

3D structure databases

ProteinModelPortaliQ92643.
SMRiQ92643.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115343. 12 interactors.
IntActiQ92643. 4 interactors.
MINTiMINT-2813159.
STRINGi9606.ENSP00000359848.

Protein family/group databases

MEROPSiC13.005.

PTM databases

iPTMnetiQ92643.
PhosphoSitePlusiQ92643.

Polymorphism and mutation databases

BioMutaiPIGK.
DMDMi22001630.

Proteomic databases

EPDiQ92643.
MaxQBiQ92643.
PaxDbiQ92643.
PeptideAtlasiQ92643.
PRIDEiQ92643.

Protocols and materials databases

DNASUi10026.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370812; ENSP00000359848; ENSG00000142892. [Q92643-1]
GeneIDi10026.
KEGGihsa:10026.
UCSCiuc001dhk.4. human. [Q92643-1]

Organism-specific databases

CTDi10026.
DisGeNETi10026.
GeneCardsiPIGK.
HGNCiHGNC:8965. PIGK.
HPAiHPA056383.
HPA057040.
MIMi605087. gene.
neXtProtiNX_Q92643.
OpenTargetsiENSG00000142892.
PharmGKBiPA33296.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1349. Eukaryota.
COG5206. LUCA.
GeneTreeiENSGT00530000063391.
HOGENOMiHOG000204398.
HOVERGENiHBG027488.
InParanoidiQ92643.
KOiK05290.
OMAiMACNHRN.
OrthoDBiEOG091G0BL9.
PhylomeDBiQ92643.
TreeFamiTF300848.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciZFISH:ENSG00000142892-MONOMER.
ReactomeiR-HSA-162791. Attachment of GPI anchor to uPAR.

Miscellaneous databases

ChiTaRSiPIGK. human.
GeneWikiiPIGK.
GenomeRNAii10026.
PROiQ92643.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000142892.
CleanExiHS_PIGK.
ExpressionAtlasiQ92643. baseline and differential.
GenevisibleiQ92643. HS.

Family and domain databases

InterProiIPR028361. GPI_transamidase.
IPR001096. Peptidase_C13.
[Graphical view]
PANTHERiPTHR12000. PTHR12000. 1 hit.
PTHR12000:SF1. PTHR12000:SF1. 1 hit.
PfamiPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFiPIRSF500138. GPI8. 1 hit.
PIRSF019663. Legumain. 1 hit.
PRINTSiPR00776. HEMOGLOBNASE.
ProtoNetiSearch...

Entry informationi

Entry nameiGPI8_HUMAN
AccessioniPrimary (citable) accession number: Q92643
Secondary accession number(s): B2R7K3
, B4E2M3, O14822, Q5TG77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.