Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q92643 (GPI8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GPI-anchor transamidase

Short name=GPI transamidase
EC=3.-.-.-
Alternative name(s):
GPI8 homolog
Short name=hGPI8
Phosphatidylinositol-glycan biosynthesis class K protein
Short name=PIG-K
Gene names
Name:PIGK
Synonyms:GPI8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein.

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subunit structure

Forms a complex with PIGT, PIGS, PIGU and GAA1. Ref.7 Ref.8

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.8.

Post-translational modification

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.

Sequence similarities

Belongs to the peptidase C13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.8
Chain28 – 395368GPI-anchor transamidase
PRO_0000026529

Regions

Topological domain28 – 367340Lumenal Potential
Transmembrane368 – 38821Helical; Potential
Topological domain389 – 3957Cytoplasmic Potential
Region311 – 32111Essential for GPI attachment

Sites

Active site1641 Ref.7
Active site2061 Ref.7

Amino acid modifications

Disulfide bond92Interchain (with C-182 in PIGT) Ref.9

Natural variations

Natural variant161T → A.
Corresponds to variant rs12723684 [ dbSNP | Ensembl ].
VAR_051518

Experimental info

Mutagenesis921C → A: Partial loss of activity. Ref.7 Ref.9
Mutagenesis921C → S: Decrease in activity. Ref.7 Ref.9
Mutagenesis1641H → A: Loss of activity. Ref.7
Mutagenesis2061C → A: Loss of activity. Ref.7
Mutagenesis311 – 39585Missing: Loss of activity. Ref.7
Sequence conflict1 – 44MAVT → SLHEA Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q92643 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: AF706DDDAD13EFB2

FASTA39545,252
        10         20         30         40         50         60 
MAVTDSLSRA ATVLATVLLL SFGSVAASHI EDQAEQFFRS GHTNNWAVLV CTSRFWFNYR 

        70         80         90        100        110        120 
HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA TVFSHKNMEL NVYGDDVEVD 

       130        140        150        160        170        180 
YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL SDDRSNILIY MTGHGGNGFL KFQDSEEITN 

       190        200        210        220        230        240 
IELADAFEQM WQKRRYNELL FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA 

       250        260        270        280        290        300 
IGVHLMDRYT FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI 

       310        320        330        340        350        360 
TDFFGSVRKV EITTETIKLQ QDSEIMESSY KEDQMDEKLM EPLKYAEQLP VAQIIHQKPK 

       370        380        390 
LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF 

« Hide

References

« Hide 'large scale' references
[1]"Yeast Gpi8p is essential for GPI anchor attachment onto proteins."
Benghezal M., Benachour A., Rusconi S., Aebi M., Conzelmann A.
EMBO J. 15:6575-6583(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The affected gene underlying the class K glycosylphosphatidylinositol (GPI) surface protein defect codes for the GPI transamidase."
Yu J., Nagarajan S., Knez J.J., Udenfriend S., Chen R., Medof M.E.
Proc. Natl. Acad. Sci. U.S.A. 94:12580-12585(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cerebellum.
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[7]"Gaa1p and gpi8p are components of a glycosylphosphatidylinositol (GPI) transamidase that mediates attachment of GPI to proteins."
Ohishi K., Inoue N., Maeda Y., Takeda J., Riezman H., Kinoshita T.
Mol. Biol. Cell 11:1523-1533(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, ACTIVE SITE, MUTAGENESIS OF CYS-92; HIS-164; CYS-206 AND 311-GLU--PHE-395.
[8]"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8."
Ohishi K., Inoue N., Kinoshita T.
EMBO J. 20:4088-4098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-47, SUBUNIT, SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.
[9]"Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge."
Ohishi K., Nagamune K., Maeda Y., Kinoshita T.
J. Biol. Chem. 278:13959-13967(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BOND FORMATION WITH PIGT, MUTAGENESIS OF CYS-92.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y07596 mRNA. Translation: CAA68871.1.
AF022913 mRNA. Translation: AAB81597.1.
AK313015 mRNA. Translation: BAG35850.1.
AL035409, AC093433, AC113935 Genomic DNA. Translation: CAI21819.1.
CH471059 Genomic DNA. Translation: EAX06380.1.
BC020737 mRNA. Translation: AAH20737.1.
CCDSCCDS674.1.
RefSeqNP_005473.1. NM_005482.2.
UniGeneHs.178305.

3D structure databases

ProteinModelPortalQ92643.
SMRQ92643. Positions 43-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115343. 9 interactions.
IntActQ92643. 1 interaction.
MINTMINT-2813159.
STRING9606.ENSP00000359848.

Protein family/group databases

MEROPSC13.005.

PTM databases

PhosphoSiteQ92643.

Polymorphism databases

DMDM22001630.

Proteomic databases

MaxQBQ92643.
PaxDbQ92643.
PRIDEQ92643.

Protocols and materials databases

DNASU10026.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370812; ENSP00000359848; ENSG00000142892.
GeneID10026.
KEGGhsa:10026.
UCSCuc001dhk.3. human.

Organism-specific databases

CTD10026.
GeneCardsGC01M077554.
HGNCHGNC:8965. PIGK.
HPAHPA056383.
HPA057040.
MIM605087. gene.
neXtProtNX_Q92643.
PharmGKBPA33296.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5206.
HOGENOMHOG000204398.
HOVERGENHBG027488.
InParanoidQ92643.
KOK05290.
OMANVLSMYR.
OrthoDBEOG70W3DZ.
PhylomeDBQ92643.
TreeFamTF300848.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.
UniPathwayUPA00196.

Gene expression databases

ArrayExpressQ92643.
BgeeQ92643.
CleanExHS_PIGK.
GenevestigatorQ92643.

Family and domain databases

InterProIPR028361. GPI_transamidase.
IPR001096. Peptidase_C13.
[Graphical view]
PANTHERPTHR12000. PTHR12000. 1 hit.
PfamPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFPIRSF500138. GPI8. 1 hit.
PIRSF019663. Legumain. 1 hit.
PRINTSPR00776. HEMOGLOBNASE.
ProtoNetSearch...

Other

ChiTaRSPIGK. human.
GeneWikiPIGK.
GenomeRNAi10026.
NextBio37889.
PROQ92643.
SOURCESearch...

Entry information

Entry nameGPI8_HUMAN
AccessionPrimary (citable) accession number: Q92643
Secondary accession number(s): B2R7K3, O14822, Q5TG77
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM