Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q92626 (PXDN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxidasin homolog

EC=1.11.1.7
Alternative name(s):
Melanoma-associated antigen MG50
Vascular peroxidase 1
p53-responsive gene 2 protein
Gene names
Name:PXDN
Synonyms:KIAA0230, MG50, PRG2, VPO, VPO1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Displays low peroxidase activity and is likely to participate in H2O2 metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. Ref.3 Ref.9

Catalytic activity

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O. Ref.3 Ref.9

Cofactor

Binds 1 calcium ion per subunit By similarity. Ref.3

Binds 1 heme B (iron-protoporphyrin IX) group covalently per subunit. Ref.3

Subcellular location

Secretedextracellular spaceextracellular matrix. Note: Enriched in the peritubular space of fibrotic kidneys. Ref.8 Ref.9

Tissue specificity

Expressed at higher levels in heart, lung, ovary, spleen, intestine and placenta, and at lower levels in liver, colon, pancreas, kidney, thymus, skeletal muscle and prostate. Expressed in tumors such as melanoma, breast cancer, ovarian cancer and glioblastoma. A shorter form probably lacking the signal sequence is found in testis and in EB1 cells undergoing p53/TP53-dependent apoptosis. Ref.1 Ref.2 Ref.3 Ref.9

Developmental stage

Expressed in fetal liver and spleen. Ref.2

Induction

By TGFB1 in fibroblasts and up-regulated in apoptotic cells. Ref.1 Ref.9

Sequence similarities

Belongs to the peroxidase family. XPO subfamily.

Contains 4 Ig-like C2-type (immunoglobulin-like) domains.

Contains 4 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 LRRNT domain.

Contains 1 VWFC domain.

Biophysicochemical properties

Kinetic parameters:

KM=0.15 mM for H2O2 Ref.3

Sequence caution

The sequence AAF06354.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA13219.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processHydrogen peroxide
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainImmunoglobulin domain
Leucine-rich repeat
Repeat
Signal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
Peroxidase
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processextracellular matrix organization

Inferred from direct assay Ref.9. Source: UniProtKB

hydrogen peroxide catabolic process

Inferred from direct assay Ref.3. Source: UniProtKB

immune response

Non-traceable author statement Ref.2. Source: UniProtKB

negative regulation of cytokine-mediated signaling pathway

Non-traceable author statement Ref.2. Source: GOC

oxidation-reduction process

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentendoplasmic reticulum

Inferred from direct assay Ref.9. Source: UniProtKB

extracellular matrix

Inferred from direct assay Ref.9. Source: UniProtKB

extracellular space

Inferred from direct assay Ref.3. Source: UniProtKB

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionextracellular matrix structural constituent

Inferred from direct assay Ref.9. Source: UniProtKB

heme binding

Inferred from direct assay Ref.3. Source: UniProtKB

interleukin-1 receptor antagonist activity

Non-traceable author statement Ref.2. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

peroxidase activity

Inferred from direct assay Ref.3Ref.9. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q92626-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q92626-2)

The sequence of this isoform differs from the canonical sequence as follows:
     703-727: YHYNDLVSPQYLNLIANLSGCTAHR → QCQSLFFLLHGLSNGVEHASVKSHS
     728-1479: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 14791453Peroxidasin homolog
PRO_0000319619

Regions

Domain27 – 6337LRRNT
Repeat87 – 10822LRR 1
Repeat111 – 13222LRR 2
Repeat135 – 15622LRR 3
Repeat159 – 18022LRR 4
Domain192 – 24554LRRCT
Domain246 – 33287Ig-like C2-type 1
Domain342 – 42887Ig-like C2-type 2
Domain433 – 52088Ig-like C2-type 3
Domain521 – 61090Ig-like C2-type 4
Domain1413 – 147159VWFC

Sites

Active site8271Proton acceptor By similarity
Metal binding8281Calcium By similarity
Metal binding9071Calcium By similarity
Metal binding9091Calcium; via carbonyl oxygen By similarity
Metal binding9111Calcium By similarity
Metal binding9131Calcium By similarity
Metal binding10741Iron (heme axial ligand) By similarity
Binding site8261Heme (covalent; via 2 links) By similarity
Binding site9801Heme (covalent; via 2 links) By similarity
Site9771Transition state stabilizer By similarity

Amino acid modifications

Modified residue11761Phosphotyrosine Ref.7
Modified residue11801Phosphoserine Ref.7
Glycosylation6401N-linked (GlcNAc...) Potential
Glycosylation6991N-linked (GlcNAc...) Potential
Glycosylation7191N-linked (GlcNAc...) Potential
Glycosylation7311N-linked (GlcNAc...) Potential
Glycosylation8651N-linked (GlcNAc...) Potential
Glycosylation11781N-linked (GlcNAc...) Ref.10
Glycosylation12801N-linked (GlcNAc...) Potential
Glycosylation13681N-linked (GlcNAc...) Potential
Glycosylation14251N-linked (GlcNAc...) Potential
Disulfide bond267 ↔ 317 By similarity
Disulfide bond363 ↔ 412 By similarity
Disulfide bond454 ↔ 502 By similarity
Disulfide bond546 ↔ 594 By similarity
Disulfide bond732 ↔ 748 By similarity
Disulfide bond847 ↔ 857 By similarity
Disulfide bond851 ↔ 875 By similarity
Disulfide bond959 ↔ 970 By similarity
Disulfide bond1177 ↔ 1234 By similarity
Disulfide bond1275 ↔ 1301 By similarity

Natural variations

Alternative sequence703 – 72725YHYND…CTAHR → QCQSLFFLLHGLSNGVEHAS VKSHS in isoform 2.
VSP_031516
Alternative sequence728 – 1479752Missing in isoform 2.
VSP_031517
Natural variant11981R → Q.
Corresponds to variant rs6723697 [ dbSNP | Ensembl ].
VAR_050487
Natural variant12611Q → R.
Corresponds to variant rs6723697 [ dbSNP | Ensembl ].
VAR_039048

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 7A75F533D89C6AAD

FASTA1,479165,275
        10         20         30         40         50         60 
MAKRSRGPGR RCLLALVLFC AWGTLAVVAQ KPGAGCPSRC LCFRTTVRCM HLLLEAVPAV 

        70         80         90        100        110        120 
APQTSILDLR FNRIREIQPG AFRRLRNLNT LLLNNNQIKR IPSGAFEDLE NLKYLYLYKN 

       130        140        150        160        170        180 
EIQSIDRQAF KGLASLEQLY LHFNQIETLD PDSFQHLPKL ERLFLHNNRI THLVPGTFNH 

       190        200        210        220        230        240 
LESMKRLRLD SNTLHCDCEI LWLADLLKTY AESGNAQAAA ICEYPRRIQG RSVATITPEE 

       250        260        270        280        290        300 
LNCERPRITS EPQDADVTSG NTVYFTCRAE GNPKPEIIWL RNNNELSMKT DSRLNLLDDG 

       310        320        330        340        350        360 
TLMIQNTQET DQGIYQCMAK NVAGEVKTQE VTLRYFGSPA RPTFVIQPQN TEVLVGESVT 

       370        380        390        400        410        420 
LECSATGHPP PRISWTRGDR TPLPVDPRVN ITPSGGLYIQ NVVQGDSGEY ACSATNNIDS 

       430        440        450        460        470        480 
VHATAFIIVQ ALPQFTVTPQ DRVVIEGQTV DFQCEAKGNP PPVIAWTKGG SQLSVDRRHL 

       490        500        510        520        530        540 
VLSSGTLRIS GVALHDQGQY ECQAVNIIGS QKVVAHLTVQ PRVTPVFASI PSDTTVEVGA 

       550        560        570        580        590        600 
NVQLPCSSQG EPEPAITWNK DGVQVTESGK FHISPEGFLT INDVGPADAG RYECVARNTI 

       610        620        630        640        650        660 
GSASVSMVLS VNVPDVSRNG DPFVATSIVE AIATVDRAIN STRTHLFDSR PRSPNDLLAL 

       670        680        690        700        710        720 
FRYPRDPYTV EQARAGEIFE RTLQLIQEHV QHGLMVDLNG TSYHYNDLVS PQYLNLIANL 

       730        740        750        760        770        780 
SGCTAHRRVN NCSDMCFHQK YRTHDGTCNN LQHPMWGASL TAFERLLKSV YENGFNTPRG 

       790        800        810        820        830        840 
INPHRLYNGH ALPMPRLVST TLIGTETVTP DEQFTHMLMQ WGQFLDHDLD STVVALSQAR 

       850        860        870        880        890        900 
FSDGQHCSNV CSNDPPCFSV MIPPNDSRAR SGARCMFFVR SSPVCGSGMT SLLMNSVYPR 

       910        920        930        940        950        960 
EQINQLTSYI DASNVYGSTE HEARSIRDLA SHRGLLRQGI VQRSGKPLLP FATGPPTECM 

       970        980        990       1000       1010       1020 
RDENESPIPC FLAGDHRANE QLGLTSMHTL WFREHNRIAT ELLKLNPHWD GDTIYYETRK 

      1030       1040       1050       1060       1070       1080 
IVGAEIQHIT YQHWLPKILG EVGMRTLGEY HGYDPGINAG IFNAFATAAF RFGHTLVNPL 

      1090       1100       1110       1120       1130       1140 
LYRLDENFQP IAQDHLPLHK AFFSPFRIVN EGGIDPLLRG LFGVAGKMRV PSQLLNTELT 

      1150       1160       1170       1180       1190       1200 
ERLFSMAHTV ALDLAAINIQ RGRDHGIPPY HDYRVYCNLS AAHTFEDLKN EIKNPEIREK 

      1210       1220       1230       1240       1250       1260 
LKRLYGSTLN IDLFPALVVE DLVPGSRLGP TLMCLLSTQF KRLRDGDRLW YENPGVFSPA 

      1270       1280       1290       1300       1310       1320 
QLTQIKQTSL ARILCDNADN ITRVQSDVFR VAEFPHGYGS CDEIPRVDLR VWQDCCEDCR 

      1330       1340       1350       1360       1370       1380 
TRGQFNAFSY HFRGRRSLEF SYQEDKPTKK TRPRKIPSVG RQGEHLSNST SAFSTRSDAS 

      1390       1400       1410       1420       1430       1440 
GTNDFREFVL EMQKTITDLR TQIKKLESRL STTECVDAGG ESHANNTKWK KDACTICECK 

      1450       1460       1470 
DGQVTCFVEA CPPATCAVPV NIPGACCPVC LQKRAEEKP 

« Hide

Isoform 2 [UniParc].

Checksum: 16391EA8B7F61878
Show »

FASTA72780,329

References

« Hide 'large scale' references
[1]"Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene."
Horikoshi N., Cong J., Kley N., Shenk T.
Biochem. Biophys. Res. Commun. 261:864-869(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, TISSUE SPECIFICITY.
[2]"A novel melanoma gene (MG50) encoding the interleukin 1 receptor antagonist and six epitopes recognized by human cytolytic T lymphocytes."
Mitchell M.S., Kan-Mitchell J., Minev B., Edman C., Deans R.J.
Cancer Res. 60:6448-6456(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]"Identification and characterization of VPO1, a new animal heme-containing peroxidase."
Cheng G., Salerno J.C., Cao Z., Pagano P.J., Lambeth J.D.
Free Radic. Biol. Med. 45:1682-1694(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), 3D-STRUCTURE MODELING, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, HEME COFACTOR, TISSUE SPECIFICITY.
[4]"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."
Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N.
DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-1479 (ISOFORM 2).
Tissue: Chondrosarcoma.
[7]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1176 AND SER-1180, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]Cheng G.
Unpublished observations (FEB-2008)
Cited for: SUBCELLULAR LOCATION.
[9]"Peroxidasin is secreted and incorporated into the extracellular matrix of myofibroblasts and fibrotic kidney."
Peterfi Z., Donko A., Orient A., Sum A., Prokai A., Molnar B., Vereb Z., Rajnavolgyi E., Kovacs K.J., Muller V., Szabo A.J., Geiszt M.
Am. J. Pathol. 175:725-735(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY TGFB1.
[10]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1178.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF200348 mRNA. Translation: AAF06354.1. Different initiation.
EF090903 mRNA. Translation: ABO25865.1.
D86983 mRNA. Translation: BAA13219.1. Different initiation.
CH471053 Genomic DNA. Translation: EAX01084.1.
CH471053 Genomic DNA. Translation: EAX01085.1.
BC098579 mRNA. Translation: AAH98579.1.
RefSeqNP_036425.1. NM_012293.1.
UniGeneHs.332197.

3D structure databases

ProteinModelPortalQ92626.
SMRQ92626. Positions 33-676, 738-1314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113596. 4 interactions.
IntActQ92626. 1 interaction.
MINTMINT-4931004.
STRING9606.ENSP00000252804.

Protein family/group databases

PeroxiBase3355. HsPxd01.

PTM databases

PhosphoSiteQ92626.

Polymorphism databases

DMDM172045828.

Proteomic databases

PaxDbQ92626.
PRIDEQ92626.

Protocols and materials databases

DNASU7837.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252804; ENSP00000252804; ENSG00000130508. [Q92626-1]
GeneID7837.
KEGGhsa:7837.
UCSCuc002qxa.3. human. [Q92626-1]
uc002qxb.1. human. [Q92626-2]

Organism-specific databases

CTD7837.
GeneCardsGC02M001635.
HGNCHGNC:14966. PXDN.
HPAHPA012375.
MIM605158. gene.
neXtProtNX_Q92626.
Orphanet289499. Congenital cataract microcornea with corneal opacity.
PharmGKBPA128394535.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG262194.
HOGENOMHOG000016084.
HOVERGENHBG108312.
InParanoidQ92626.
OMAEPVITWN.
OrthoDBEOG7D2FD6.
PhylomeDBQ92626.
TreeFamTF314316.

Gene expression databases

ArrayExpressQ92626.
BgeeQ92626.
CleanExHS_PRG2.
HS_PXDN.
GenevestigatorQ92626.

Family and domain databases

Gene3D1.10.640.10. 2 hits.
2.60.40.10. 4 hits.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR010255. Haem_peroxidase.
IPR002007. Haem_peroxidase_animal.
IPR019791. Haem_peroxidase_animal_subgr.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR001007. VWF_C.
[Graphical view]
PfamPF03098. An_peroxidase. 1 hit.
PF07679. I-set. 4 hits.
PF00093. VWC. 1 hit.
[Graphical view]
PRINTSPR00457. ANPEROXIDASE.
SMARTSM00408. IGc2. 4 hits.
SM00369. LRR_TYP. 4 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF48113. SSF48113. 1 hit.
PROSITEPS50835. IG_LIKE. 4 hits.
PS51450. LRR. 5 hits.
PS50292. PEROXIDASE_3. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPXDN. human.
GeneWikiPXDN.
GenomeRNAi7837.
NextBio30238.
PROQ92626.
SOURCESearch...

Entry information

Entry namePXDN_HUMAN
AccessionPrimary (citable) accession number: Q92626
Secondary accession number(s): A8QM65, D6W4Y0, Q4KMG2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM