Q92625 (ANS1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ankyrin repeat and SAM domain-containing protein 1A

Alternative name(s):
Odin

Gene names

Name:	ANKS1A
Synonyms:	ANKS1, KIAA0229, ODIN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction By similarity. Ref.6
Subunit structure	Interacts with EPHA8; EPHA8 kinase activity-independent but stimulated by EPHA8 ubiquitination By similarity.
Subcellular location	Cytoplasm. Cell projection By similarity. Note: Cytoplasmic before and after growth factor treatment.
Tissue specificity	Widely expressed (at protein level).
Post-translational modification	Phosphorylated on tyrosine residues in response to EGF and PDGF By similarity.
Sequence similarities	Contains 6 ANK repeats. Contains 1 PID domain. Contains 2 SAM (sterile alpha motif) domains.
Sequence caution	The sequence BAA13218.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ephrin receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB neuron remodeling Inferred from sequence or structural similarity. Source: UniProtKB substrate-dependent cell migration Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA neuron projection Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 1134

1133

Ankyrin repeat and SAM domain-containing protein 1A

PRO_0000066921

Regions

Repeat

79 – 108

30

ANK 1

Repeat

112 – 141

30

ANK 2

Repeat

148 – 177

30

ANK 3

Repeat

181 – 210

30

ANK 4

Repeat

214 – 243

30

ANK 5

Repeat

246 – 275

30

ANK 6

Domain

696 – 762

67

SAM 1

Domain

770 – 837

68

SAM 2

Domain

936 – 1091

156

PID

Compositional bias

2 – 55

54

Gly-rich

Amino acid modifications

Modified residue

2

1

N-acetylglycine Ref.5

Modified residue

318

1

Phosphothreonine Ref.8

Modified residue

622

1

Phosphoserine Ref.8

Modified residue

624

1

Phosphoserine By similarity

Modified residue

626

1

Phosphoserine By similarity

Modified residue

628

1

Phosphoserine By similarity

Modified residue

647

1

Phosphoserine Ref.8 Ref.12

Modified residue

661

1

Phosphoserine Ref.8 Ref.10 Ref.12

Modified residue

663

1

Phosphoserine Ref.9 Ref.10 Ref.12

Modified residue

666

1

Phosphoserine By similarity

Modified residue

887

1

Phosphoserine By similarity

Natural variations

Natural variant

355

1

A → D.
Corresponds to variant rs6930932 [ dbSNP | Ensembl ].

VAR_048282

Natural variant

694

1

L → S. Ref.1 Ref.3 Ref.4 Ref.9
Corresponds to variant rs820085 [ dbSNP | Ensembl ].

VAR_021168

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1134

Helix Strand Turn

Details...

Helix

701 – 708

8

Helix

711 – 713

3

Helix

714 – 719

6

Turn

728 – 730

3

Helix

735 – 741

7

Helix

746 – 757

12

Beta strand

759 – 761

3

Sequences

	Sequence		Length	Mass (Da)
	Q92625 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: E519844AD56EE414	FASTA	1,134	123,108
	10 20 30 40 50 60 MGKEQELLEA ARTGHLPAVE KLLSGKRLSS GFGGGGGGGS GGGGGGSGGG GGGLGSSSHP 70 80 90 100 110 120 LSSLLSMWRG PNVNCVDSTG YTPLHHAALN GHKDVVEVLL RNDALTNVAD SKGCYPLHLA 130 140 150 160 170 180 AWKGDAQIVR LLIHQGPSHT RVNEQNNDNE TALHCAAQYG HTEVVKVLLE ELTDPTMRNN 190 200 210 220 230 240 KFETPLDLAA LYGRLEVVKM LLNAHPNLLS CNTKKHTPLH LAARNGHKAV VQVLLDAGMD 250 260 270 280 290 300 SNYQTEMGSA LHEAALFGKT DVVQILLAAG TDVNIKDNHG LTALDTVREL PSQKSQQIAA 310 320 330 340 350 360 LIEDHMTGKR STKEVDKTPP PQPPLISSMD SISQKSQGDV EKAVTELIID FDANAEEEGP 370 380 390 400 410 420 YEALYNAISC HSLDSMASGR SSDQDSTNKE AEAAGVKPAG VRPRERPPPP AKPPPDEEEE 430 440 450 460 470 480 DHIDKKYFPL TASEVLSMRP RIHGSAAREE DEHPYELLLT AETKKVVLVD GKTKDHRRSS 490 500 510 520 530 540 SSRSQDSAEG QDGQVPEQFS GLLHGSSPVC EVGQDPFQLL CTAGQSHPDG SPQQGACHKA 550 560 570 580 590 600 SMQLEETGVH APGASQPSAL DQSKRVGYLT GLPTTNSRSH PETLTHTASP HPGGAEEGDR 610 620 630 640 650 660 SGARSRAPPT SKPKAELKLS RSLSKSDSDL LTCSPTEDAT MGSRSESLSN CSIGKKRLEK 670 680 690 700 710 720 SPSFASEWDE IEKIMSSIGE GIDFSQERQK ISGLRTLEQS VGEWLESIGL QQYESKLLLN 730 740 750 760 770 780 GFDDVHFLGS NVMEEQDLRD IGISDPQHRR KLLQAARSLP KVKALGYDGN SPPSVPSWLD 790 800 810 820 830 840 SLGLQDYVHS FLSSGYSSID TVKNLWELEL VNVLKVQLLG HRKRIIASLA DRPYEEPPQK 850 860 870 880 890 900 PPRFSQLRCQ DLLSQTSSPL SQNDSCTGRS ADLLLPPGDT GRRRHDSLHD PAAPSRAERF 910 920 930 940 950 960 RIQEEHREAK LTLRPPSLAA PYAPVQSWQH QPEKLIFESC GYEANYLGSM LIKDLRGTES 970 980 990 1000 1010 1020 TQDACAKMRK STEHMKKIPT IILSITYKGV KFIDASNKNV IAEHEIRNIS CAAQDPEDLC 1030 1040 1050 1060 1070 1080 TFAYITKDLQ TSHHYCHVFS TVDVNLTYEI ILTLGQAFEV AYQLALQAQK SRATGASAAE 1090 1100 1110 1120 1130 MIETKSSKPV PKPRVGVRKS ALEPPDMDQD AQSHASVSWV VDPKPDSKRS LSTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain." Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N. DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-694. Tissue: Bone marrow.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT SER-694.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-694.
[5]	"Cloning of a novel phosphotyrosine binding domain containing molecule, Odin, involved in signaling by receptor tyrosine kinases." Pandey A., Blagoev B., Kratchmarova I., Fernandez M., Nielsen M., Kristiansen T.Z., Ohara O., Podtelejnikov A.V., Roche S., Lodish H.F., Mann M. Oncogene 21:8029-8036(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 540-564, ACETYLATION AT GLY-2, MASS SPECTROMETRY, CHARACTERIZATION. Tissue: Cervix carcinoma.
[6]	"Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function." Shin J., Gu C., Park E., Park S. Mol. Cell. Biol. 27:8113-8126(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN EFNA5-EPHA8 SIGNALING PATHWAY.
[7]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Platelet.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-318; SER-622; SER-647 AND SER-661, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-663, VARIANT [LARGE SCALE ANALYSIS] SER-694, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-661 AND SER-663, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-647; SER-661 AND SER-663, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D86982 mRNA. Translation: BAA13218.1. Different initiation.
AL138721, AL033520, AL591002 Genomic DNA. Translation: CAI12437.1.
AL591002, AL033520, AL138721 Genomic DNA. Translation: CAI15138.1.
AL033520, AL138721, AL591002 Genomic DNA. Translation: CAI20281.1.
CH471081 Genomic DNA. Translation: EAX03804.1.
BC132832 mRNA. Translation: AAI32833.1.

IPI

IPI00395663.

RefSeq

NP_056060.2. NM_015245.2.

UniGene

Hs.656492.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LMR	NMR	-	A	691-770	[»]

ProteinModelPortal

Q92625.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1493202.

STRING

9606.ENSP00000353518.

PTM databases

PhosphoSite

Q92625.

Polymorphism databases

DMDM

62511243.

Proteomic databases

PaxDb

Q92625.

PeptideAtlas

Q92625.

PRIDE

Q92625.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000360359; ENSP00000353518; ENSG00000064999.

GeneID

23294.

KEGG

hsa:23294.

UCSC

uc003ojx.4. human.

Organism-specific databases

CTD

23294.

GeneCards

GC06P034857.

HGNC

HGNC:20961. ANKS1A.

HPA

HPA036768.
HPA036769.

MIM

608994. gene.

neXtProt

NX_Q92625.

PharmGKB

PA134958476.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0666.

HOGENOM

HOG000033973.

HOVERGEN

HBG050506.

InParanoid

Q92625.

OMA

HLPAVEK.

OrthoDB

EOG4PVNXV.

PhylomeDB

Q92625.

Gene expression databases

ArrayExpress

Q92625.

Bgee

Q92625.

CleanEx

HS_ANKS1A.

Genevestigator

Q92625.

GermOnline

ENSG00000064999. Homo sapiens.

Family and domain databases

Gene3D

1.10.150.50. 2 hits.
1.25.40.20. 4 hits.
2.30.29.30. 1 hit.

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR011993. PH_like_dom.
IPR006020. PTyr_interaction_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
[Graphical view]

Pfam

PF00023. Ank. 5 hits.
PF00640. PID. 1 hit.
PF00536. SAM_1. 2 hits.
[Graphical view]

PRINTS

PR01415. ANKYRIN.

SMART

SM00248. ANK. 6 hits.
SM00462. PTB. 1 hit.
SM00454. SAM. 2 hits.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.
SSF47769. SAM_homology. 2 hits.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 6 hits.
PS01179. PID. 1 hit.
PS50105. SAM_DOMAIN. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ANKS1A. human.

GenomeRNAi

23294.

NextBio

45120.

SOURCE

Search...

Entry information

Entry name

ANS1A_HUMAN

Accession

Primary (citable) accession number: Q92625
Secondary accession number(s): A2RUC1 , Q5JYI9, Q5SYR2, Q86WQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 114 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.