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Protein

Zinc finger protein 516

Gene

ZNF516

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that binds to the promoter and activates the transcription of genes promoting brown adipose tissue (BAT) differentiation. Among brown adipose tissue-specific genes, binds the proximal region of the promoter of the UCP1 gene to activate its transcription and thereby regulate thermogenesis (By similarity). May also play a role in the cellular response to replication stress (PubMed:23446422).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri62 – 8423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19724C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri200 – 22324C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri276 – 29823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri335 – 35723C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri515 – 53723C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri760 – 78324C2H2-type 9; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1098 – 112023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 516Imported
Gene namesi
Name:ZNF516Imported
Synonyms:KIAA0222Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:28990. ZNF516.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134992707.

Polymorphism and mutation databases

BioMutaiZNF516.
DMDMi14548318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11631163Zinc finger protein 516PRO_0000047634Add
BLAST

Proteomic databases

EPDiQ92618.
MaxQBiQ92618.
PaxDbiQ92618.
PRIDEiQ92618.

PTM databases

iPTMnetiQ92618.
PhosphoSiteiQ92618.

Expressioni

Gene expression databases

BgeeiQ92618.
CleanExiHS_ZNF516.
ExpressionAtlasiQ92618. baseline and differential.
GenevisibleiQ92618. HS.

Organism-specific databases

HPAiHPA015792.

Interactioni

Subunit structurei

Interacts with PRDM16; the interaction is direct and may play a role in the transcription of brown adipose tissue-specific genes.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115016. 9 interactions.
IntActiQ92618. 5 interactions.
MINTiMINT-1728539.
STRINGi9606.ENSP00000394757.

Structurei

3D structure databases

ProteinModelPortaliQ92618.
SMRiQ92618. Positions 34-84, 172-357, 508-546, 786-823, 1090-1135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 431431Mediates promoter DNA-binding and activation of transcriptionBy similarityAdd
BLAST

Sequence similaritiesi

Contains 10 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri62 – 8423C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri174 – 19724C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri200 – 22324C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri248 – 27023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri276 – 29823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri335 – 35723C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri515 – 53723C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri760 – 78324C2H2-type 9; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1098 – 112023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063100.
HOGENOMiHOG000155800.
HOVERGENiHBG079384.
InParanoidiQ92618.
OMAiKYGAWDE.
OrthoDBiEOG76471T.
PhylomeDBiQ92618.
TreeFamiTF332241.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRNREAEME LRRGPSPTRA GRGHEVDGDK ATCHTCCICG KSFPFQSSLS
60 70 80 90 100
QHMRKHTGEK PYKCPYCDHR ASQKGNLKIH IRSHRTGTLI QGHEPEAGEA
110 120 130 140 150
PLGEMRASEG LDACASPTKS ASACNRLLNG ASQADGARVL NGASQADSGR
160 170 180 190 200
VLLRSSKKGA EGSACAPGEA KAAVQCSFCK SQFERKKDLE LHVHQAHKPF
210 220 230 240 250
KCRLCSYATL REESLLSHIE RDHITAQGPG SGEACVENGK PELSPGEFPC
260 270 280 290 300
EVCGQAFSQT WFLKAHMKKH RGSFDHGCHI CGRRFKEPWF LKNHMKAHGP
310 320 330 340 350
KTGSKNRPKS ELDPIATINN VVQEEVIVAG LSLYEVCAKC GNLFTNLDSL
360 370 380 390 400
NAHNAIHRRV EASRTRAPAE EGAEGPSDTK QFFLQCLNLR PSAAGDSCPG
410 420 430 440 450
TQAGRRVAEL DPVNSYQAWQ LATRGKVAEP AEYLKYGAWD EALAGDVAFD
460 470 480 490 500
KDRREYVLVS QEKRKREQDA PAAQGPPRKR ASGPGDPAPA GHLDPRSAAR
510 520 530 540 550
PNRRAAATTG QGKSSECFEC GKIFRTYHQM VLHSRVHRRA RRERDSDGDR
560 570 580 590 600
AARARCGSLS EGDSASQPSS PGSACAAADS PGSGLADEAA EDSGEEGAPE
610 620 630 640 650
PAPGGQPRRC CFSEEVTSTE LSSGDQSHKM GDNASERDTG ESKAGIAASV
660 670 680 690 700
SILENSSRET SRRQEQHRFS MDLKMPAFHP KQEVPVPGDG VEFPSSTGAE
710 720 730 740 750
GQTGHPAEKL SDLHNKEHSG GGKRALAPDL MPLDLSARST RDDPSNKETA
760 770 780 790 800
SSLQAALVVH PCPYCSHKTY YPEVLWMHKR IWHRVSCNSV APPWIQPNGY
810 820 830 840 850
KSIRSNLVFL SRSGRTGPPP ALGGKECQPL LLARFTRTQV PGGMPGSKSG
860 870 880 890 900
SSPLGVVTKA ASMPKNKESH SGGPCALWAP GPDGYRQTKP CHGQEPHGAA
910 920 930 940 950
TQGPLAKPRQ EASSKPVPAP GGGGFSRSAT PTPTVIARAG AQPSANSKPV
960 970 980 990 1000
EKFGVPPAGA GFAPTNKHSA PDSLKAKFSA QPQGPPPAKG EGGAPPLPPR
1010 1020 1030 1040 1050
EPPSKAAQEL RTLATCAAGS RGDAALQAQP GVAGAPPVLH SIKQEPVAEG
1060 1070 1080 1090 1100
HEKRLDILNI FKTYIPKDFA TLYQGWGVSG PGLEHRGTLR TQARPGEFVC
1110 1120 1130 1140 1150
IECGKSFHQP GHLRAHMRAH SVVFESDGPR GSEVHTTSAD APKQGRDHSN
1160
TGTVQTVPLR KGT
Length:1,163
Mass (Da):124,289
Last modified:February 1, 1997 - v1
Checksum:i9D6DDDE2D70236A4
GO

Sequence cautioni

The sequence BAA13211.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41N → S.
Corresponds to variant rs3752097 [ dbSNP | Ensembl ].
VAR_052852
Natural varianti239 – 2391G → S.
Corresponds to variant rs12961584 [ dbSNP | Ensembl ].
VAR_052853

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86975 mRNA. Translation: BAA13211.2. Different initiation.
CCDSiCCDS74234.1.
RefSeqiNP_055458.1. NM_014643.3.
XP_011524571.1. XM_011526269.1.
XP_011524572.1. XM_011526270.1.
XP_011524573.1. XM_011526271.1.
XP_011524574.1. XM_011526272.1.
XP_011524575.1. XM_011526273.1.
XP_011524576.1. XM_011526274.1.
XP_011524577.1. XM_011526275.1.
UniGeneiHs.436973.

Genome annotation databases

EnsembliENST00000443185; ENSP00000394757; ENSG00000101493.
GeneIDi9658.
KEGGihsa:9658.
UCSCiuc032hhr.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86975 mRNA. Translation: BAA13211.2. Different initiation.
CCDSiCCDS74234.1.
RefSeqiNP_055458.1. NM_014643.3.
XP_011524571.1. XM_011526269.1.
XP_011524572.1. XM_011526270.1.
XP_011524573.1. XM_011526271.1.
XP_011524574.1. XM_011526272.1.
XP_011524575.1. XM_011526273.1.
XP_011524576.1. XM_011526274.1.
XP_011524577.1. XM_011526275.1.
UniGeneiHs.436973.

3D structure databases

ProteinModelPortaliQ92618.
SMRiQ92618. Positions 34-84, 172-357, 508-546, 786-823, 1090-1135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115016. 9 interactions.
IntActiQ92618. 5 interactions.
MINTiMINT-1728539.
STRINGi9606.ENSP00000394757.

PTM databases

iPTMnetiQ92618.
PhosphoSiteiQ92618.

Polymorphism and mutation databases

BioMutaiZNF516.
DMDMi14548318.

Proteomic databases

EPDiQ92618.
MaxQBiQ92618.
PaxDbiQ92618.
PRIDEiQ92618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000443185; ENSP00000394757; ENSG00000101493.
GeneIDi9658.
KEGGihsa:9658.
UCSCiuc032hhr.2. human.

Organism-specific databases

CTDi9658.
GeneCardsiZNF516.
HGNCiHGNC:28990. ZNF516.
HPAiHPA015792.
MIMi615114. gene.
neXtProtiNX_Q92618.
PharmGKBiPA134992707.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063100.
HOGENOMiHOG000155800.
HOVERGENiHBG079384.
InParanoidiQ92618.
OMAiKYGAWDE.
OrthoDBiEOG76471T.
PhylomeDBiQ92618.
TreeFamiTF332241.

Miscellaneous databases

ChiTaRSiZNF516. human.
GenomeRNAii9658.
PROiQ92618.
SOURCEiSearch...

Gene expression databases

BgeeiQ92618.
CleanExiHS_ZNF516.
ExpressionAtlasiQ92618. baseline and differential.
GenevisibleiQ92618. HS.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain."
    Nagase T., Seki N., Ishikawa K., Ohira M., Kawarabayasi Y., Ohara O., Tanaka A., Kotani H., Miyajima N., Nomura N.
    DNA Res. 3:321-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Bone marrow.
  2. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  3. Cited for: FUNCTION.
  4. "Cold-inducible Zfp516 activates UCP1 transcription to promote browning of white fat and development of brown fat."
    Dempersmier J., Sambeat A., Gulyaeva O., Paul S.M., Hudak C.S., Raposo H.F., Kwan H.Y., Kang C., Wong R.H., Sul H.S.
    Mol. Cell 57:235-246(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRDM16.

Entry informationi

Entry nameiZN516_HUMAN
AccessioniPrimary (citable) accession number: Q92618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.